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The map label for this gene is ppnK

Identifier: 23098676

GI number: 23098676

Start: 1270716

End: 1271519

Strand: Direct

Name: ppnK

Synonym: OB1221

Alternate gene names: 23098676

Gene position: 1270716-1271519 (Clockwise)

Preceding gene: 23098675

Following gene: 23098677

Centisome position: 35.0

GC content: 36.69

Gene sequence:

>804_bases
ATGAACTTTAAAATTGTTTCAAAAGGTGATGACCGTTCTGAAAAAATAAAAGCAATGATGCGTCAATATTTATCAGAATT
TGGATTGACTTATGATAAAGAGACCCCAGATTTAGTAATTTCCGTTGGGGGAGACGGTACATTCCTTGAAGCATTTCATC
GCTATGTTCATCGATTAGAAGACACTGCTTTTATTGGAATACATACTGGTCATTTAGGATTTTATACAGATTGGACACCT
AAAGATGTGGAAAGACTGATTATAGAGATTGCAAAAACGCCATTTCAAACCGTTGAATACCCTTTATTAGAAGTCATTAT
TAGAGCGAAAGCAGGCGGGAAAGAAGATCGTATTCTTGCTTTAAATGAAGCGATGATAAAAACAGCGGACGGTTCTTCCG
TTGTATTTGATGTAGAAATAAAAGGCGAGCATTTTGAAACATTTCGCGGAGACGGAATCTGTATATCCACACCTTCAGGA
AGCACAGCATACAATAAAGCTCTCGGAGGGGCGATTTTGCATCCATCATTAGAAGCAATTCAAATTACGGAGAATGCATC
CATTAATAATCGTGTTTTCCGTACTATTGGTTCGCCGCTTATACTACCAAAACATCATACTTGTTTCTTAAAACCAATGG
TAGATAGCAGTTTTCTTATTCAAATTGATCATTTTACAAAGAATTATCAAAATGTAAAATCGATACAGTGTAGAGTAGCT
AAAGAGAAGGTGCGGTTTGCTCGTTTTAAGCAATTCCCATTCTGGAACAGAGTTAGAGATTCCTTTGTATCGGAGGAAGG
GTAA

Upstream 100 bases:

>100_bases
AGCCTTTAAATTAGATGAAGAAATGTCTAAAATTAGAAGTGAAATTCATGAAGCGCAAAGAGTGTTTCATCGTAAATAAT
AGTTGAAGGAGAAATAATAC

Downstream 100 bases:

>100_bases
TGTGATTTTACGGTGGGAAATCAAGAAAAATGAGCAAGGAATGTTGATTAAAACCTATTTACAACAAATTCATCATTTTT
CTAGGAGATTACTTATTTCT

Product: inorganic polyphosphate/ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase 1

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MNFKIVSKGDDRSEKIKAMMRQYLSEFGLTYDKETPDLVISVGGDGTFLEAFHRYVHRLEDTAFIGIHTGHLGFYTDWTP
KDVERLIIEIAKTPFQTVEYPLLEVIIRAKAGGKEDRILALNEAMIKTADGSSVVFDVEIKGEHFETFRGDGICISTPSG
STAYNKALGGAILHPSLEAIQITENASINNRVFRTIGSPLILPKHHTCFLKPMVDSSFLIQIDHFTKNYQNVKSIQCRVA
KEKVRFARFKQFPFWNRVRDSFVSEEG

Sequences:

>Translated_267_residues
MNFKIVSKGDDRSEKIKAMMRQYLSEFGLTYDKETPDLVISVGGDGTFLEAFHRYVHRLEDTAFIGIHTGHLGFYTDWTP
KDVERLIIEIAKTPFQTVEYPLLEVIIRAKAGGKEDRILALNEAMIKTADGSSVVFDVEIKGEHFETFRGDGICISTPSG
STAYNKALGGAILHPSLEAIQITENASINNRVFRTIGSPLILPKHHTCFLKPMVDSSFLIQIDHFTKNYQNVKSIQCRVA
KEKVRFARFKQFPFWNRVRDSFVSEEG
>Mature_267_residues
MNFKIVSKGDDRSEKIKAMMRQYLSEFGLTYDKETPDLVISVGGDGTFLEAFHRYVHRLEDTAFIGIHTGHLGFYTDWTP
KDVERLIIEIAKTPFQTVEYPLLEVIIRAKAGGKEDRILALNEAMIKTADGSSVVFDVEIKGEHFETFRGDGICISTPSG
STAYNKALGGAILHPSLEAIQITENASINNRVFRTIGSPLILPKHHTCFLKPMVDSSFLIQIDHFTKNYQNVKSIQCRVA
KEKVRFARFKQFPFWNRVRDSFVSEEG

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family

Homologues:

Organism=Escherichia coli, GI1788968, Length=173, Percent_Identity=34.1040462427746, Blast_Score=77, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6325068, Length=168, Percent_Identity=27.3809523809524, Blast_Score=66, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPNK1_OCEIH (Q8ERS9)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_692142.1
- ProteinModelPortal:   Q8ERS9
- SMR:   Q8ERS9
- GeneID:   1017393
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB1221
- NMPDR:   fig|221109.1.peg.1220
- HOGENOM:   HBG314020
- OMA:   PSIRAIQ
- ProtClustDB:   PRK04885
- BioCyc:   OIHE221109:OB1221-MONOMER
- BRENDA:   2.7.1.23
- GO:   GO:0005737
- HAMAP:   MF_00361
- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504
- Gene3D:   G3DSA:2.60.200.30
- Gene3D:   G3DSA:3.40.50.10330
- PANTHER:   PTHR20275

Pfam domain/function: PF01513 NAD_kinase; SSF111331 ATP-NAD_kinase_PpnK-typ

EC number: =2.7.1.23

Molecular weight: Translated: 30240; Mature: 30240

Theoretical pI: Translated: 7.69; Mature: 7.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFKIVSKGDDRSEKIKAMMRQYLSEFGLTYDKETPDLVISVGGDGTFLEAFHRYVHRLE
CCEEEECCCCCHHHHHHHHHHHHHHHHCCEECCCCCCEEEEECCCCHHHHHHHHHHHHHC
DTAFIGIHTGHLGFYTDWTPKDVERLIIEIAKTPFQTVEYPLLEVIIRAKAGGKEDRILA
CCEEEEEEECCCCEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCEEE
LNEAMIKTADGSSVVFDVEIKGEHFETFRGDGICISTPSGSTAYNKALGGAILHPSLEAI
ECHHHEEECCCCEEEEEEEECCCHHHHCCCCCEEEECCCCCCHHHHHCCCEEECCCCEEE
QITENASINNRVFRTIGSPLILPKHHTCFLKPMVDSSFLIQIDHFTKNYQNVKSIQCRVA
EEECCCCCCHHHHHHCCCCEEECCCCEEEEEECCCCEEEEEEECHHCCHHHHHHHHHHHH
KEKVRFARFKQFPFWNRVRDSFVSEEG
HHHHHHHHHHCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MNFKIVSKGDDRSEKIKAMMRQYLSEFGLTYDKETPDLVISVGGDGTFLEAFHRYVHRLE
CCEEEECCCCCHHHHHHHHHHHHHHHHCCEECCCCCCEEEEECCCCHHHHHHHHHHHHHC
DTAFIGIHTGHLGFYTDWTPKDVERLIIEIAKTPFQTVEYPLLEVIIRAKAGGKEDRILA
CCEEEEEEECCCCEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCEEE
LNEAMIKTADGSSVVFDVEIKGEHFETFRGDGICISTPSGSTAYNKALGGAILHPSLEAI
ECHHHEEECCCCEEEEEEEECCCHHHHCCCCCEEEECCCCCCHHHHHCCCEEECCCCEEE
QITENASINNRVFRTIGSPLILPKHHTCFLKPMVDSSFLIQIDHFTKNYQNVKSIQCRVA
EEECCCCCCHHHHHHCCCCEEECCCCEEEEEECCCCEEEEEEECHHCCHHHHHHHHHHHH
KEKVRFARFKQFPFWNRVRDSFVSEEG
HHHHHHHHHHCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376