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The map label for this gene is gsiB [H]

Identifier: 23098228

GI number: 23098228

Start: 826208

End: 827800

Strand: Direct

Name: gsiB [H]

Synonym: OB0773

Alternate gene names: 23098228

Gene position: 826208-827800 (Clockwise)

Preceding gene: 23098227

Following gene: 23098229

Centisome position: 22.76

GC content: 39.61

Gene sequence:

>1593_bases
ATGAAATCGCGTAAAGTTTTAGTCGGTTTTATCATAACACTTGTGTTTAGTTTATTGCTTGCTGCATGTGCCAGTGAGCC
AGAAGAAAATAGTGATAGTAACTCAGAAAAAAGTACGAGTCAGGGTGGGGACTTAGTAATTGCTAGTCAAGCAGATGCGG
TATCATTAGATCCTCATGCAACAAACGATACACCTTCTGCAAATGTTCGCATTAATATATTTGATAATTTGGTTACACAA
AATGAAGATATGGAGCTTCAGCCTTCTCTAGCAGAAAGTTGGGAGCAAGTAAATGATACAACATGGGAATTTAAGTTGCG
AGAAGACGTCACTTTTCATGACGGATCTGTCTTCAATTCAAATGTAGTGAAAGCAAATATAGAACGTATACTAGATCCTG
ACATAGGGTCACCAGTAGCATTTATGTACGATATGATAACAGAGGTACAAGTGGTTGATGATTATACGGTACGCTTTATC
ACTGAATATCCTTTTGCTCCGTTGCCAGCTCATTTAGCACATCCGGGTGGACAGATGGTGTCGCAAGAATTAATTGAAGA
GGATTATGCAGCAATGGAGAATGGGGAAGAACCAAGCAGTGTAATTAATTCAAACCCAGTGGGTACTGGTCCTTTTACAT
TTGAGAATTGGCAAACTGGTGAATCCGTGAAGCTAATGAAAAATGAGGATTACTGGGATAAACCAGCAATGCTTAATTCT
GTTACCTTTAAAGTAGTTTCAGAGGATTTAACTCGAATCTCTGAATTATCTACGGGTGATTCTCATATTATTACTCCTTT
AAGCCCATCTGATGTTGCAGAAGTTGAAAATACAGATGGAATGAATGTGCAACGCCAGGAAAGTTCTTCTTTAGCTTATA
TAGGTTTCAATATGGAAAAGGAACCATTTGATGATGTGCGTGTACGACAAGCAATTTCGATGGCTATCAACAAAGAAGAA
ATCATTAACGGAATTTATGAAGGTGCTGGCATTCCAGCCAAGGGCCCACTAGCTCCTGGGATTTTTGGATATGATGAGCA
ATTAAATGGTTTGGAATACAATGTTGAAGAGGCAAAATCTTTATTAGAAGAAGCGGGTTATCCCGATGGATTCTCTGCGA
CGATCTGGACGAATGATGATCGTCAACGGATTGATACAGCAACAAATGTTCAAGCTCAACTTGCGGAAATCGGAATCGAC
TTAGAGGTAGAAACGGTTGAATGGGGGGCTATGCTGGATCAAACTGCTAAGGGCGAACATGAGATGATGGTATTTGGTTG
GACGACTGTTACCGGCGATGCAGATAATGGCATGTATCCACTATTCCATTCTGAAAATGTTGGATCACCAGGAAATCGCA
CATTTACAGTAGATAAAGAATTGGATTCTTACTTGGAAGAAGCACGTCAAACAGCTGACCCTGAAAAGCGTCTAGATTTA
TATAGTAAGGCACAGGAAAAGCTAGTTGAGCTTTCTCCATTTGTGTACTTACTTCATCAAGAATATTTACTTGGAGTTCG
TGATGAGGTGAAGGGCCTTACCCAGTTGCCAACGCAGTTATTGCAATTAAAGAATGTTTCTTTAGAAGATTAA

Upstream 100 bases:

>100_bases
TCCGATTTAATTGCAACAAAAGAACTAGTGAAAGCATTTATATTTAACTTAAATGAAGATACAAAATTTACATTTTAATA
CATAAATGGGGGAAATCAGC

Downstream 100 bases:

>100_bases
ATAATATAAAGTCTGATGTCCACTGGTTGGATATTGGGCTTTTTTAAATGGGTTAGTATTAAGCAACTTATTTTAATAAC
AGAACAATATATTGTTGTAA

Product: oligopeptide ABC transporter oligopeptide-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 530; Mature: 530

Protein sequence:

>530_residues
MKSRKVLVGFIITLVFSLLLAACASEPEENSDSNSEKSTSQGGDLVIASQADAVSLDPHATNDTPSANVRINIFDNLVTQ
NEDMELQPSLAESWEQVNDTTWEFKLREDVTFHDGSVFNSNVVKANIERILDPDIGSPVAFMYDMITEVQVVDDYTVRFI
TEYPFAPLPAHLAHPGGQMVSQELIEEDYAAMENGEEPSSVINSNPVGTGPFTFENWQTGESVKLMKNEDYWDKPAMLNS
VTFKVVSEDLTRISELSTGDSHIITPLSPSDVAEVENTDGMNVQRQESSSLAYIGFNMEKEPFDDVRVRQAISMAINKEE
IINGIYEGAGIPAKGPLAPGIFGYDEQLNGLEYNVEEAKSLLEEAGYPDGFSATIWTNDDRQRIDTATNVQAQLAEIGID
LEVETVEWGAMLDQTAKGEHEMMVFGWTTVTGDADNGMYPLFHSENVGSPGNRTFTVDKELDSYLEEARQTADPEKRLDL
YSKAQEKLVELSPFVYLLHQEYLLGVRDEVKGLTQLPTQLLQLKNVSLED

Sequences:

>Translated_530_residues
MKSRKVLVGFIITLVFSLLLAACASEPEENSDSNSEKSTSQGGDLVIASQADAVSLDPHATNDTPSANVRINIFDNLVTQ
NEDMELQPSLAESWEQVNDTTWEFKLREDVTFHDGSVFNSNVVKANIERILDPDIGSPVAFMYDMITEVQVVDDYTVRFI
TEYPFAPLPAHLAHPGGQMVSQELIEEDYAAMENGEEPSSVINSNPVGTGPFTFENWQTGESVKLMKNEDYWDKPAMLNS
VTFKVVSEDLTRISELSTGDSHIITPLSPSDVAEVENTDGMNVQRQESSSLAYIGFNMEKEPFDDVRVRQAISMAINKEE
IINGIYEGAGIPAKGPLAPGIFGYDEQLNGLEYNVEEAKSLLEEAGYPDGFSATIWTNDDRQRIDTATNVQAQLAEIGID
LEVETVEWGAMLDQTAKGEHEMMVFGWTTVTGDADNGMYPLFHSENVGSPGNRTFTVDKELDSYLEEARQTADPEKRLDL
YSKAQEKLVELSPFVYLLHQEYLLGVRDEVKGLTQLPTQLLQLKNVSLED
>Mature_530_residues
MKSRKVLVGFIITLVFSLLLAACASEPEENSDSNSEKSTSQGGDLVIASQADAVSLDPHATNDTPSANVRINIFDNLVTQ
NEDMELQPSLAESWEQVNDTTWEFKLREDVTFHDGSVFNSNVVKANIERILDPDIGSPVAFMYDMITEVQVVDDYTVRFI
TEYPFAPLPAHLAHPGGQMVSQELIEEDYAAMENGEEPSSVINSNPVGTGPFTFENWQTGESVKLMKNEDYWDKPAMLNS
VTFKVVSEDLTRISELSTGDSHIITPLSPSDVAEVENTDGMNVQRQESSSLAYIGFNMEKEPFDDVRVRQAISMAINKEE
IINGIYEGAGIPAKGPLAPGIFGYDEQLNGLEYNVEEAKSLLEEAGYPDGFSATIWTNDDRQRIDTATNVQAQLAEIGID
LEVETVEWGAMLDQTAKGEHEMMVFGWTTVTGDADNGMYPLFHSENVGSPGNRTFTVDKELDSYLEEARQTADPEKRLDL
YSKAQEKLVELSPFVYLLHQEYLLGVRDEVKGLTQLPTQLLQLKNVSLED

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=534, Percent_Identity=30.1498127340824, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI1789966, Length=519, Percent_Identity=29.8651252408478, Blast_Score=244, Evalue=1e-65,
Organism=Escherichia coli, GI1787762, Length=492, Percent_Identity=28.6585365853659, Blast_Score=212, Evalue=6e-56,
Organism=Escherichia coli, GI1787551, Length=456, Percent_Identity=26.3157894736842, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1787495, Length=505, Percent_Identity=24.1584158415842, Blast_Score=141, Evalue=8e-35,
Organism=Escherichia coli, GI1789397, Length=496, Percent_Identity=23.991935483871, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI1789887, Length=300, Percent_Identity=25.3333333333333, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI87081878, Length=523, Percent_Identity=23.3269598470363, Blast_Score=102, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 58829; Mature: 58829

Theoretical pI: Translated: 3.96; Mature: 3.96

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSRKVLVGFIITLVFSLLLAACASEPEENSDSNSEKSTSQGGDLVIASQADAVSLDPHA
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCEEECCCCC
TNDTPSANVRINIFDNLVTQNEDMELQPSLAESWEQVNDTTWEFKLREDVTFHDGSVFNS
CCCCCCCEEEEEEEHHHHCCCCCCCCCCHHHHHHHHHCCCEEEEEEECCCEECCCCCCCC
NVVKANIERILDPDIGSPVAFMYDMITEVQVVDDYTVRFITEYPFAPLPAHLAHPGGQMV
CHHHHHHHHHCCCCCCCHHHHHHHHHHHEEEECCCEEEEEECCCCCCCCHHHCCCCHHHH
SQELIEEDYAAMENGEEPSSVINSNPVGTGPFTFENWQTGESVKLMKNEDYWDKPAMLNS
HHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCEECCCCCCCCEEEEECCCCCCCCHHHHH
VTFKVVSEDLTRISELSTGDSHIITPLSPSDVAEVENTDGMNVQRQESSSLAYIGFNMEK
HHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHCCCCCCCCEEECCCCCEEEEECCCCC
EPFDDVRVRQAISMAINKEEIINGIYEGAGIPAKGPLAPGIFGYDEQLNGLEYNVEEAKS
CCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCCCEECHHHHHH
LLEEAGYPDGFSATIWTNDDRQRIDTATNVQAQLAEIGIDLEVETVEWGAMLDQTAKGEH
HHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHCCCCCC
EMMVFGWTTVTGDADNGMYPLFHSENVGSPGNRTFTVDKELDSYLEEARQTADPEKRLDL
EEEEEEEEEEECCCCCCEECEEECCCCCCCCCEEEEECHHHHHHHHHHHHCCCHHHHHHH
YSKAQEKLVELSPFVYLLHQEYLLGVRDEVKGLTQLPTQLLQLKNVSLED
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKSRKVLVGFIITLVFSLLLAACASEPEENSDSNSEKSTSQGGDLVIASQADAVSLDPHA
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCEEECCCCC
TNDTPSANVRINIFDNLVTQNEDMELQPSLAESWEQVNDTTWEFKLREDVTFHDGSVFNS
CCCCCCCEEEEEEEHHHHCCCCCCCCCCHHHHHHHHHCCCEEEEEEECCCEECCCCCCCC
NVVKANIERILDPDIGSPVAFMYDMITEVQVVDDYTVRFITEYPFAPLPAHLAHPGGQMV
CHHHHHHHHHCCCCCCCHHHHHHHHHHHEEEECCCEEEEEECCCCCCCCHHHCCCCHHHH
SQELIEEDYAAMENGEEPSSVINSNPVGTGPFTFENWQTGESVKLMKNEDYWDKPAMLNS
HHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCEECCCCCCCCEEEEECCCCCCCCHHHHH
VTFKVVSEDLTRISELSTGDSHIITPLSPSDVAEVENTDGMNVQRQESSSLAYIGFNMEK
HHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHCCCCCCCCEEECCCCCEEEEECCCCC
EPFDDVRVRQAISMAINKEEIINGIYEGAGIPAKGPLAPGIFGYDEQLNGLEYNVEEAKS
CCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCCCEECHHHHHH
LLEEAGYPDGFSATIWTNDDRQRIDTATNVQAQLAEIGIDLEVETVEWGAMLDQTAKGEH
HHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHCCCCCC
EMMVFGWTTVTGDADNGMYPLFHSENVGSPGNRTFTVDKELDSYLEEARQTADPEKRLDL
EEEEEEEEEEECCCCCCEECEEECCCCCCCCCEEEEECHHHHHHHHHHHHCCCHHHHHHH
YSKAQEKLVELSPFVYLLHQEYLLGVRDEVKGLTQLPTQLLQLKNVSLED
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA