Click here to switch to the map view.

The map label for this gene is proS

Identifier: 23098021

GI number: 23098021

Start: 595508

End: 596944

Strand: Direct

Name: proS

Synonym: OB0566

Alternate gene names: 23098021

Gene position: 595508-596944 (Clockwise)

Preceding gene: 23098020

Following gene: 23098022

Centisome position: 16.4

GC content: 38.48

Gene sequence:

>1437_bases
GTGGGAAAGAAAAATAAACAGTTCGTAGAAAAAATTACTGCGATGGAAGATGATTTTGCACAATGGTATACCGACGTTGT
AAAGCAAGCAGACTTGGTAGACTATGGACAAGTTAGAGGAACAATGATTATAAAACCGTATGGTTATGCAATTTGGGAAA
ATATCAAGAATGAATTGGATCGTATGTTTAAAGAAACGGGACATACAAATGTTGCCTTTCCTTTATTTATACCAGAAAGT
TTGCTTCAAAAAGAGAAGGATCATGTAGAAGGATTTGCTCCTGAAGTTGCATGGGTAACCCATGGTGGAGAAGAAGAATT
GACCGAACGTATTGCGGTTCGTCCAACATCAGAAGTGCTTTTCTGTGATTACTATTCCAATAATATTCATTCGTATCGTG
ATCTTCCAAAACTTTATAATCAATGGGGAAATGTAGTTCGTTGGGAAAAGACAACACGTCCATTCTTACGTTCTTCTGAA
TTCCATTGGCAAGAAGGTCATACTGCACATGCAACAGATGAAGAAGCAACCGAAGAAACAAATCGCATGTTAGAAACCTA
TGCAAAACTTGTAGAAGAGTATTTAGCTGTCCCAGTCCTAAAAGGAAGAAAGACAGATAAAGAAAAATTCGCTGGTGCAA
AATTCACGCTTACGATTGAAGCATTGATGCATGACGGGAAAGCTTTGCAATCAGGAACGTCTCATCATTTTGGAACCGGA
TTTGCAGAAGCGTTTGATATTAATTATCTTGATAAAGATGGCAAGAGTCAATTTGTTCACCAAACTTCTTGGGGATTATC
GACGCGTATTATGGGTGCTTTAATTATGGTACATGGAGATAATCGTGGACTTGTTGTTCCTCCAAAAATTGCTCCAACCC
AGGCGATGATTGTTCCGATTGCCCAACATAAAGAAGGCGTACTTGATAAAGCTTACGAACTACGTGATAAATTAGCTAAG
GTTGCGCGTGTGGATATTGACGGCAGCGATAAGATGCCAGGGTGGAAGTTTAATGAGTATGAAATGAAGGGTATCCCTGT
TCGTGTAGAGATGGGTCCAAAAGATATCGAAAAAGAACAGGTTGTACTTGTACGACGTGATACGGGTGAGAAAGAGTTCG
TACCAGTAGCAGAAGTAGAAACTCGTTTAGTTGAATTGCTTGATGAGGTTCAATCGAATTTATATCAACGTGCACTAGAT
CATCGAAATGAGATGACTACTGTTGCAAAAGATATGGATGAATTTAAAGAGAAAATAGAAGAAGGCGGATTTATTAAAGC
AATGTGGTGTGGAGATGTAAGTTGTGAGGAATCCATTAAAGAAGAAACAACCGCAACTTCTCGTTGCATTCCTTATGAAG
AAGAAAAAGTAGCAGATACTTGTGTATGCTGTGGTAAAAAAGCAAAAGAATTAGTATATTGGGCTAGAGCATACTAA

Upstream 100 bases:

>100_bases
ATATACTGGGCTTTGATTGGAAGGGAAAGTGTCGTCTTCGAATTTTATACAAGATCATATTATGAAATTGATTCAAATAG
ATGAAAATGAGGTGTAGGAA

Downstream 100 bases:

>100_bases
GCTTTTGTAAATTAAAGTTGTGGCTTGGATAAAAGTATTATTGTCCCAGCCACAACTTTTTAATTGTCTAGCTGTGATAA
ACGGGCGCTTGCGCTTTTGT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MGKKNKQFVEKITAMEDDFAQWYTDVVKQADLVDYGQVRGTMIIKPYGYAIWENIKNELDRMFKETGHTNVAFPLFIPES
LLQKEKDHVEGFAPEVAWVTHGGEEELTERIAVRPTSEVLFCDYYSNNIHSYRDLPKLYNQWGNVVRWEKTTRPFLRSSE
FHWQEGHTAHATDEEATEETNRMLETYAKLVEEYLAVPVLKGRKTDKEKFAGAKFTLTIEALMHDGKALQSGTSHHFGTG
FAEAFDINYLDKDGKSQFVHQTSWGLSTRIMGALIMVHGDNRGLVVPPKIAPTQAMIVPIAQHKEGVLDKAYELRDKLAK
VARVDIDGSDKMPGWKFNEYEMKGIPVRVEMGPKDIEKEQVVLVRRDTGEKEFVPVAEVETRLVELLDEVQSNLYQRALD
HRNEMTTVAKDMDEFKEKIEEGGFIKAMWCGDVSCEESIKEETTATSRCIPYEEEKVADTCVCCGKKAKELVYWARAY

Sequences:

>Translated_478_residues
MGKKNKQFVEKITAMEDDFAQWYTDVVKQADLVDYGQVRGTMIIKPYGYAIWENIKNELDRMFKETGHTNVAFPLFIPES
LLQKEKDHVEGFAPEVAWVTHGGEEELTERIAVRPTSEVLFCDYYSNNIHSYRDLPKLYNQWGNVVRWEKTTRPFLRSSE
FHWQEGHTAHATDEEATEETNRMLETYAKLVEEYLAVPVLKGRKTDKEKFAGAKFTLTIEALMHDGKALQSGTSHHFGTG
FAEAFDINYLDKDGKSQFVHQTSWGLSTRIMGALIMVHGDNRGLVVPPKIAPTQAMIVPIAQHKEGVLDKAYELRDKLAK
VARVDIDGSDKMPGWKFNEYEMKGIPVRVEMGPKDIEKEQVVLVRRDTGEKEFVPVAEVETRLVELLDEVQSNLYQRALD
HRNEMTTVAKDMDEFKEKIEEGGFIKAMWCGDVSCEESIKEETTATSRCIPYEEEKVADTCVCCGKKAKELVYWARAY
>Mature_477_residues
GKKNKQFVEKITAMEDDFAQWYTDVVKQADLVDYGQVRGTMIIKPYGYAIWENIKNELDRMFKETGHTNVAFPLFIPESL
LQKEKDHVEGFAPEVAWVTHGGEEELTERIAVRPTSEVLFCDYYSNNIHSYRDLPKLYNQWGNVVRWEKTTRPFLRSSEF
HWQEGHTAHATDEEATEETNRMLETYAKLVEEYLAVPVLKGRKTDKEKFAGAKFTLTIEALMHDGKALQSGTSHHFGTGF
AEAFDINYLDKDGKSQFVHQTSWGLSTRIMGALIMVHGDNRGLVVPPKIAPTQAMIVPIAQHKEGVLDKAYELRDKLAKV
ARVDIDGSDKMPGWKFNEYEMKGIPVRVEMGPKDIEKEQVVLVRRDTGEKEFVPVAEVETRLVELLDEVQSNLYQRALDH
RNEMTTVAKDMDEFKEKIEEGGFIKAMWCGDVSCEESIKEETTATSRCIPYEEEKVADTCVCCGKKAKELVYWARAY

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro)

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily

Homologues:

Organism=Homo sapiens, GI62241042, Length=492, Percent_Identity=44.5121951219512, Blast_Score=429, Evalue=1e-120,
Organism=Escherichia coli, GI1786392, Length=165, Percent_Identity=28.4848484848485, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71991342, Length=489, Percent_Identity=39.8773006134969, Blast_Score=393, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI71991346, Length=357, Percent_Identity=44.2577030812325, Blast_Score=339, Evalue=2e-93,
Organism=Saccharomyces cerevisiae, GI6321808, Length=495, Percent_Identity=42.8282828282828, Blast_Score=422, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24649468, Length=493, Percent_Identity=40.5679513184584, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24649466, Length=493, Percent_Identity=40.5679513184584, Blast_Score=390, Evalue=1e-109,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_OCEIH (Q8ESQ5)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_691487.1
- HSSP:   O26708
- ProteinModelPortal:   Q8ESQ5
- SMR:   Q8ESQ5
- GeneID:   1015888
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB0566
- NMPDR:   fig|221109.1.peg.569
- HOGENOM:   HBG334108
- OMA:   PGWKFNE
- ProtClustDB:   PRK08661
- BioCyc:   OIHE221109:OB0566-MONOMER
- BRENDA:   6.1.1.15
- GO:   GO:0005737
- HAMAP:   MF_01571
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004499
- InterPro:   IPR017449
- InterPro:   IPR016061
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.30.110.30
- PANTHER:   PTHR11451:SF6
- PRINTS:   PR01046
- SMART:   SM00946
- TIGRFAMs:   TIGR00408

Pfam domain/function: PF03129 HGTP_anticodon; PF09180 ProRS-C_1; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd; SSF64586 Pro-tRNA_synth_II_C

EC number: =6.1.1.15

Molecular weight: Translated: 54751; Mature: 54619

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKKNKQFVEKITAMEDDFAQWYTDVVKQADLVDYGQVRGTMIIKPYGYAIWENIKNELD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCEEEECCCCHHHHHHHHHHHH
RMFKETGHTNVAFPLFIPESLLQKEKDHVEGFAPEVAWVTHGGEEELTERIAVRPTSEVL
HHHHHCCCCCEEEEEECCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHCCCCCCEE
FCDYYSNNIHSYRDLPKLYNQWGNVVRWEKTTRPFLRSSEFHWQEGHTAHATDEEATEET
EEEECCCCCHHHHHHHHHHHHHCCEEEECHHCCHHHCCCCCCCCCCCCCCCCCHHHHHHH
NRMLETYAKLVEEYLAVPVLKGRKTDKEKFAGAKFTLTIEALMHDGKALQSGTSHHFGTG
HHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCEEEEEHHHHHCCCHHHHCCCCCCCCCC
FAEAFDINYLDKDGKSQFVHQTSWGLSTRIMGALIMVHGDNRGLVVPPKIAPTQAMIVPI
CHHHEECCCCCCCCCHHHHHHHCCCHHHHHEEEEEEEEECCCCEEECCCCCCCCEEEEEH
AQHKEGVLDKAYELRDKLAKVARVDIDGSDKMPGWKFNEYEMKGIPVRVEMGPKDIEKEQ
HHHHCHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCEEECCCEEEEECCCCCCCCCE
VVLVRRDTGEKEFVPVAEVETRLVELLDEVQSNLYQRALDHRNEMTTVAKDMDEFKEKIE
EEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EGGFIKAMWCGDVSCEESIKEETTATSRCIPYEEEKVADTCVCCGKKAKELVYWARAY
CCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCC
>Mature Secondary Structure 
GKKNKQFVEKITAMEDDFAQWYTDVVKQADLVDYGQVRGTMIIKPYGYAIWENIKNELD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCEEEECCCCHHHHHHHHHHHH
RMFKETGHTNVAFPLFIPESLLQKEKDHVEGFAPEVAWVTHGGEEELTERIAVRPTSEVL
HHHHHCCCCCEEEEEECCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHCCCCCCEE
FCDYYSNNIHSYRDLPKLYNQWGNVVRWEKTTRPFLRSSEFHWQEGHTAHATDEEATEET
EEEECCCCCHHHHHHHHHHHHHCCEEEECHHCCHHHCCCCCCCCCCCCCCCCCHHHHHHH
NRMLETYAKLVEEYLAVPVLKGRKTDKEKFAGAKFTLTIEALMHDGKALQSGTSHHFGTG
HHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCEEEEEHHHHHCCCHHHHCCCCCCCCCC
FAEAFDINYLDKDGKSQFVHQTSWGLSTRIMGALIMVHGDNRGLVVPPKIAPTQAMIVPI
CHHHEECCCCCCCCCHHHHHHHCCCHHHHHEEEEEEEEECCCCEEECCCCCCCCEEEEEH
AQHKEGVLDKAYELRDKLAKVARVDIDGSDKMPGWKFNEYEMKGIPVRVEMGPKDIEKEQ
HHHHCHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCEEECCCEEEEECCCCCCCCCE
VVLVRRDTGEKEFVPVAEVETRLVELLDEVQSNLYQRALDHRNEMTTVAKDMDEFKEKIE
EEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EGGFIKAMWCGDVSCEESIKEETTATSRCIPYEEEKVADTCVCCGKKAKELVYWARAY
CCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376