Click here to switch to the map view.

The map label for this gene is murB

Identifier: 23098011

GI number: 23098011

Start: 587739

End: 588662

Strand: Direct

Name: murB

Synonym: OB0556

Alternate gene names: 23098011

Gene position: 587739-588662 (Clockwise)

Preceding gene: 23098010

Following gene: 23098016

Centisome position: 16.19

GC content: 38.64

Gene sequence:

>924_bases
ATGAAAGTCAGCCATCATCTATATGATCAACTATCACGTATCACTTCTGAGGAAAATGTGATGGTCGATGAATTAATCAG
AAATCATACGTATACGAAATTAGGCGGGAAAGCTGATGTATATATAACACCTGAATCGTACAGTGAAGTACAAGATATTA
TTAAATTAGCGAATAAAGAAGATGTCCCGTTTACAATGCTAGGTAATGGGTCCAATCTGATTGTAAAAGACGGCGGAATA
CGCGGGATAGTAATGAATTTACAAAAACTTGCCTCAATTTGGAGGGAAGAAGATAAGATAATTGCGCAGAGCGGAGCGCG
TATTATTGATGCATCTAGAACAGCATTAGCAGAGAGCCTTGCTGGGTTAGAATTTGCTTGTGGAATCCCCGGTTCTGTAG
GTGGAGCACTCTATATGAATGCGGGTGCCTACGGTGGAGAGATAAAAGATGTTTTAGAAAGTACGATTGTGGTGACAAAA
GAAGGGGATATTCGCACATTGACCGCTGTGGAGTTAGACCTTGATTATCGTACCAGTAATATTCCAGATAAGGGATATAT
CGTACTGGAAGCAACCTTTGCATTAAAAAAAGCAAATTCTTCCGATATTAAAGAAGTTATGGATGATTTAACGTATAAAA
GAGAATCAAAGCAACCACTTGAATATCCATCTTGTGGAAGTGTTTTTAAGCGTCCGCCGGGGTATTTTGCAGGGAAGCTA
ATTCAAGATAGTGAGCTACAAGGAACACAAATTGGTGGAGCTGAAGTATCAAAAAAACATGCTGGTTTTATCGTTAATAA
AAACAATGCCAGTGCTACAGAATACATTAATTTGATTCGTCACGTGCAAAAAACAGTTAGCGAAAAGTTTGGTGTTCAGC
TTGAACGTGAAGTAAGGATTATTGGAGAAGATCCGGTAGAATAA

Upstream 100 bases:

>100_bases
TCTTTTTTGTGGCAGGAATCAGATGATTAACTTACAATAGAATAAGATTCAAATGGACGTATCATAAATAGTAATTGAAT
TGTTTAAAGGAATGATAAAA

Downstream 100 bases:

>100_bases
TAAAAAGTGCTATCCGTTTGATTTTAACGGATAGCACTTTTTTCTTGTCTTTTTCGTTTGAAGCGATCGATTTAGCCTCT
AAAACGATGTAAGAATTCTT

Product: UDP-N-acetylenolpyruvoylglucosamine reductase

Products: NA

Alternate protein names: UDP-N-acetylmuramate dehydrogenase

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MKVSHHLYDQLSRITSEENVMVDELIRNHTYTKLGGKADVYITPESYSEVQDIIKLANKEDVPFTMLGNGSNLIVKDGGI
RGIVMNLQKLASIWREEDKIIAQSGARIIDASRTALAESLAGLEFACGIPGSVGGALYMNAGAYGGEIKDVLESTIVVTK
EGDIRTLTAVELDLDYRTSNIPDKGYIVLEATFALKKANSSDIKEVMDDLTYKRESKQPLEYPSCGSVFKRPPGYFAGKL
IQDSELQGTQIGGAEVSKKHAGFIVNKNNASATEYINLIRHVQKTVSEKFGVQLEREVRIIGEDPVE

Sequences:

>Translated_307_residues
MKVSHHLYDQLSRITSEENVMVDELIRNHTYTKLGGKADVYITPESYSEVQDIIKLANKEDVPFTMLGNGSNLIVKDGGI
RGIVMNLQKLASIWREEDKIIAQSGARIIDASRTALAESLAGLEFACGIPGSVGGALYMNAGAYGGEIKDVLESTIVVTK
EGDIRTLTAVELDLDYRTSNIPDKGYIVLEATFALKKANSSDIKEVMDDLTYKRESKQPLEYPSCGSVFKRPPGYFAGKL
IQDSELQGTQIGGAEVSKKHAGFIVNKNNASATEYINLIRHVQKTVSEKFGVQLEREVRIIGEDPVE
>Mature_307_residues
MKVSHHLYDQLSRITSEENVMVDELIRNHTYTKLGGKADVYITPESYSEVQDIIKLANKEDVPFTMLGNGSNLIVKDGGI
RGIVMNLQKLASIWREEDKIIAQSGARIIDASRTALAESLAGLEFACGIPGSVGGALYMNAGAYGGEIKDVLESTIVVTK
EGDIRTLTAVELDLDYRTSNIPDKGYIVLEATFALKKANSSDIKEVMDDLTYKRESKQPLEYPSCGSVFKRPPGYFAGKL
IQDSELQGTQIGGAEVSKKHAGFIVNKNNASATEYINLIRHVQKTVSEKFGVQLEREVRIIGEDPVE

Specific function: Cell wall formation

COG id: COG0812

COG function: function code M; UDP-N-acetylmuramate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain

Homologues:

Organism=Escherichia coli, GI1790407, Length=317, Percent_Identity=28.7066246056782, Blast_Score=93, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURB_OCEIH (Q8ESR4)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_691477.1
- ProteinModelPortal:   Q8ESR4
- SMR:   Q8ESR4
- GeneID:   1015875
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB0556
- NMPDR:   fig|221109.1.peg.559
- HOGENOM:   HBG686573
- OMA:   SKKHAGF
- ProtClustDB:   PRK13905
- BioCyc:   OIHE221109:OB0556-MONOMER
- BRENDA:   1.1.1.158
- GO:   GO:0005737
- HAMAP:   MF_00037
- InterPro:   IPR016169
- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR003170
- InterPro:   IPR011601
- InterPro:   IPR006094
- Gene3D:   G3DSA:3.30.465.10
- Gene3D:   G3DSA:3.30.43.10
- Gene3D:   G3DSA:3.90.78.10
- PANTHER:   PTHR21071
- TIGRFAMs:   TIGR00179

Pfam domain/function: PF01565 FAD_binding_4; PF02873 MurB_C; SSF56176 FAD-binding_2; SSF56194 MurB_C

EC number: =1.1.1.158

Molecular weight: Translated: 33720; Mature: 33720

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS51387 FAD_PCMH

Important sites: ACT_SITE 177-177 ACT_SITE 227-227 ACT_SITE 297-297

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVSHHLYDQLSRITSEENVMVDELIRNHTYTKLGGKADVYITPESYSEVQDIIKLANKE
CCCCHHHHHHHHHHCCCCCHHHHHHHHCCCEEECCCCEEEEECCCHHHHHHHHHHHCCCC
DVPFTMLGNGSNLIVKDGGIRGIVMNLQKLASIWREEDKIIAQSGARIIDASRTALAESL
CCCEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHH
AGLEFACGIPGSVGGALYMNAGAYGGEIKDVLESTIVVTKEGDIRTLTAVELDLDYRTSN
CCCEEECCCCCCCCCEEEEECCCCCCHHHHHHHHEEEEECCCCEEEEEEEEEEECCCCCC
IPDKGYIVLEATFALKKANSSDIKEVMDDLTYKRESKQPLEYPSCGSVFKRPPGYFAGKL
CCCCCEEEEEEEEEHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCHHCCE
IQDSELQGTQIGGAEVSKKHAGFIVNKNNASATEYINLIRHVQKTVSEKFGVQLEREVRI
ECCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEEE
IGEDPVE
ECCCCCC
>Mature Secondary Structure
MKVSHHLYDQLSRITSEENVMVDELIRNHTYTKLGGKADVYITPESYSEVQDIIKLANKE
CCCCHHHHHHHHHHCCCCCHHHHHHHHCCCEEECCCCEEEEECCCHHHHHHHHHHHCCCC
DVPFTMLGNGSNLIVKDGGIRGIVMNLQKLASIWREEDKIIAQSGARIIDASRTALAESL
CCCEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHH
AGLEFACGIPGSVGGALYMNAGAYGGEIKDVLESTIVVTKEGDIRTLTAVELDLDYRTSN
CCCEEECCCCCCCCCEEEEECCCCCCHHHHHHHHEEEEECCCCEEEEEEEEEEECCCCCC
IPDKGYIVLEATFALKKANSSDIKEVMDDLTYKRESKQPLEYPSCGSVFKRPPGYFAGKL
CCCCCEEEEEEEEEHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCHHCCE
IQDSELQGTQIGGAEVSKKHAGFIVNKNNASATEYINLIRHVQKTVSEKFGVQLEREVRI
ECCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEEE
IGEDPVE
ECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376