BacMap

Definition	Oceanobacillus iheyensis HTE831, complete genome.
Accession	NC_004193
Length	3,630,528

Click here to switch to the map view.

The map label for this gene is yqhE [C]

Identifier: 23097990

GI number: 23097990

Start: 568547

End: 569371

Strand: Direct

Name: yqhE [C]

Synonym: OB0535

Alternate gene names: 23097990

Gene position: 568547-569371 (Clockwise)

Preceding gene: 23097988

Following gene: 23097992

Centisome position: 15.66

GC content: 33.7

Gene sequence:

>825_bases
ATGGAATATGTTACTTTAAATAATGGCTTAAAAATGCCTCAATTAGGTTTTGGAGTTTGGAAAGTTCCAAATGAAGAAGT
CGTTTCACCAGTAGAACATGCATTAAAAGTAGGTTATCGTTCAATAGATACAGCAAAAGTATATGGAAATGAAGTAGGGG
TAGGAGAAGCTATCTCTAATAGCGGTATACCGCGTGAGCAATTGTTTATAACTACCAAAGTGTGGAATAGTGATCATGGG
TATGATAATACATTAAAAGCGTTCGATGAAAGTTTAGAAAGGTTAGGTTTAGACTACGTAGATCTTTACTTAATCCATTG
GCCAACTCCAGAATTTGACCAATATGTAGAAACTTATAAAGCTTTAGAAACATTATATAAAGACGGTAAAGTTAAAGCAA
TTGGTGTTTGTAATTTTGATATTGAGTATTTAGAAAGAATACTTAATGAATGTGAAATAGTACCGGTACTTAATCAAGTA
GAATGTCATCCTTATCTACAACAAAAAGAACTTCGGGATTTTTGTGAGAAACACAATATCTACCTAGAGTCGTATAGCCC
TCTAATGAATGGTAAAGAGGTACTTCAAAGTGAAGAACTACAAAACTTAGCAGAAAGAAAAGGTAAAACTGTTGCTCAAA
CAATTCTACGTTGGCATCTGCAATCAGGAATGATAGTTATTCCTAAGTCAGTGACACCTAACCGTATCGAAGAAAATCTA
GATGTATTTGATTTTGAACTTACGGATACTGAAATGCAGGAGATTAACGAATTGGATAGGAATTTAAGAAGCGGTGCAGT
ACCAAGTGAAATGAATGTAAGATAA

Upstream 100 bases:

>100_bases
AAAATTTATGAAAGATTTGATATTATAAGCTAATAAATCGTATGAAACTATCAAATAAAGTATAATTATTGATAGATTAA
GAGAAAGGGATGAGAAGACG

Downstream 100 bases:

>100_bases
ATTGTAATACGAATAATAAATAATTCATGTCCTGATATTACTTGAATCATTAAATAACACCTTCGAACTGATACATTCAA
ACGATGCAATCATTCGAAGG

Product: oxidoreductase

Products: 2-keto-L-gulonate; NADP [C]

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MEYVTLNNGLKMPQLGFGVWKVPNEEVVSPVEHALKVGYRSIDTAKVYGNEVGVGEAISNSGIPREQLFITTKVWNSDHG
YDNTLKAFDESLERLGLDYVDLYLIHWPTPEFDQYVETYKALETLYKDGKVKAIGVCNFDIEYLERILNECEIVPVLNQV
ECHPYLQQKELRDFCEKHNIYLESYSPLMNGKEVLQSEELQNLAERKGKTVAQTILRWHLQSGMIVIPKSVTPNRIEENL
DVFDFELTDTEMQEINELDRNLRSGAVPSEMNVR

Sequences:

>Translated_274_residues
MEYVTLNNGLKMPQLGFGVWKVPNEEVVSPVEHALKVGYRSIDTAKVYGNEVGVGEAISNSGIPREQLFITTKVWNSDHG
YDNTLKAFDESLERLGLDYVDLYLIHWPTPEFDQYVETYKALETLYKDGKVKAIGVCNFDIEYLERILNECEIVPVLNQV
ECHPYLQQKELRDFCEKHNIYLESYSPLMNGKEVLQSEELQNLAERKGKTVAQTILRWHLQSGMIVIPKSVTPNRIEENL
DVFDFELTDTEMQEINELDRNLRSGAVPSEMNVR
>Mature_274_residues
MEYVTLNNGLKMPQLGFGVWKVPNEEVVSPVEHALKVGYRSIDTAKVYGNEVGVGEAISNSGIPREQLFITTKVWNSDHG
YDNTLKAFDESLERLGLDYVDLYLIHWPTPEFDQYVETYKALETLYKDGKVKAIGVCNFDIEYLERILNECEIVPVLNQV
ECHPYLQQKELRDFCEKHNIYLESYSPLMNGKEVLQSEELQNLAERKGKTVAQTILRWHLQSGMIVIPKSVTPNRIEENL
DVFDFELTDTEMQEINELDRNLRSGAVPSEMNVR

Specific function: Reduction [C]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI24497577, Length=292, Percent_Identity=39.7260273972603, Blast_Score=220, Evalue=8e-58,
Organism=Homo sapiens, GI5174391, Length=292, Percent_Identity=39.7260273972603, Blast_Score=220, Evalue=8e-58,
Organism=Homo sapiens, GI223468663, Length=298, Percent_Identity=39.9328859060403, Blast_Score=208, Evalue=4e-54,
Organism=Homo sapiens, GI310109922, Length=279, Percent_Identity=40.1433691756272, Blast_Score=195, Evalue=3e-50,
Organism=Homo sapiens, GI93277124, Length=307, Percent_Identity=36.1563517915309, Blast_Score=192, Evalue=2e-49,
Organism=Homo sapiens, GI45446745, Length=297, Percent_Identity=39.0572390572391, Blast_Score=190, Evalue=9e-49,
Organism=Homo sapiens, GI4503285, Length=297, Percent_Identity=39.0572390572391, Blast_Score=190, Evalue=9e-49,
Organism=Homo sapiens, GI310109920, Length=297, Percent_Identity=38.7205387205387, Blast_Score=190, Evalue=1e-48,
Organism=Homo sapiens, GI5453543, Length=297, Percent_Identity=38.7205387205387, Blast_Score=188, Evalue=4e-48,
Organism=Homo sapiens, GI4502049, Length=297, Percent_Identity=40.4040404040404, Blast_Score=187, Evalue=9e-48,
Organism=Homo sapiens, GI24497583, Length=296, Percent_Identity=37.8378378378378, Blast_Score=181, Evalue=6e-46,
Organism=Homo sapiens, GI5174695, Length=298, Percent_Identity=35.9060402684564, Blast_Score=180, Evalue=1e-45,
Organism=Homo sapiens, GI291291012, Length=272, Percent_Identity=38.2352941176471, Blast_Score=177, Evalue=6e-45,
Organism=Homo sapiens, GI24497585, Length=297, Percent_Identity=38.047138047138, Blast_Score=176, Evalue=1e-44,
Organism=Homo sapiens, GI300116273, Length=277, Percent_Identity=35.0180505415162, Blast_Score=156, Evalue=2e-38,
Organism=Homo sapiens, GI300116271, Length=278, Percent_Identity=34.5323741007194, Blast_Score=151, Evalue=7e-37,
Organism=Homo sapiens, GI310109924, Length=154, Percent_Identity=40.9090909090909, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI207028673, Length=113, Percent_Identity=42.4778761061947, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI310109926, Length=112, Percent_Identity=41.9642857142857, Blast_Score=89, Evalue=3e-18,
Organism=Escherichia coli, GI87082198, Length=270, Percent_Identity=50.7407407407407, Blast_Score=295, Evalue=3e-81,
Organism=Escherichia coli, GI1786400, Length=256, Percent_Identity=37.890625, Blast_Score=185, Evalue=2e-48,
Organism=Escherichia coli, GI1788081, Length=272, Percent_Identity=27.2058823529412, Blast_Score=110, Evalue=7e-26,
Organism=Escherichia coli, GI1788070, Length=316, Percent_Identity=25.9493670886076, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI48994888, Length=163, Percent_Identity=29.4478527607362, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI87081735, Length=291, Percent_Identity=24.7422680412371, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17537077, Length=295, Percent_Identity=39.6610169491525, Blast_Score=206, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI17537075, Length=295, Percent_Identity=38.9830508474576, Blast_Score=201, Evalue=3e-52,
Organism=Caenorhabditis elegans, GI17564128, Length=300, Percent_Identity=35.6666666666667, Blast_Score=192, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI17550248, Length=291, Percent_Identity=37.8006872852234, Blast_Score=186, Evalue=8e-48,
Organism=Caenorhabditis elegans, GI71998625, Length=264, Percent_Identity=38.2575757575758, Blast_Score=186, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI17566692, Length=289, Percent_Identity=36.6782006920415, Blast_Score=177, Evalue=4e-45,
Organism=Caenorhabditis elegans, GI17537079, Length=297, Percent_Identity=33.3333333333333, Blast_Score=177, Evalue=7e-45,
Organism=Caenorhabditis elegans, GI17561298, Length=271, Percent_Identity=34.3173431734317, Blast_Score=174, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI17538386, Length=271, Percent_Identity=36.5313653136531, Blast_Score=173, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17562292, Length=274, Percent_Identity=35.4014598540146, Blast_Score=156, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17552492, Length=270, Percent_Identity=31.1111111111111, Blast_Score=151, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI17561300, Length=272, Percent_Identity=33.0882352941176, Blast_Score=146, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17550246, Length=79, Percent_Identity=45.5696202531646, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324694, Length=286, Percent_Identity=38.4615384615385, Blast_Score=202, Evalue=5e-53,
Organism=Saccharomyces cerevisiae, GI6319625, Length=294, Percent_Identity=42.5170068027211, Blast_Score=201, Evalue=7e-53,
Organism=Saccharomyces cerevisiae, GI6320576, Length=284, Percent_Identity=38.0281690140845, Blast_Score=198, Evalue=8e-52,
Organism=Saccharomyces cerevisiae, GI6321896, Length=307, Percent_Identity=34.8534201954397, Blast_Score=182, Evalue=7e-47,
Organism=Saccharomyces cerevisiae, GI6322556, Length=267, Percent_Identity=34.0823970037453, Blast_Score=176, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6320079, Length=282, Percent_Identity=37.2340425531915, Blast_Score=159, Evalue=6e-40,
Organism=Drosophila melanogaster, GI24644950, Length=304, Percent_Identity=39.4736842105263, Blast_Score=214, Evalue=7e-56,
Organism=Drosophila melanogaster, GI24663317, Length=279, Percent_Identity=43.3691756272401, Blast_Score=212, Evalue=2e-55,
Organism=Drosophila melanogaster, GI221378297, Length=324, Percent_Identity=37.6543209876543, Blast_Score=208, Evalue=4e-54,
Organism=Drosophila melanogaster, GI24662789, Length=292, Percent_Identity=38.3561643835616, Blast_Score=204, Evalue=5e-53,
Organism=Drosophila melanogaster, GI20129731, Length=286, Percent_Identity=39.1608391608392, Blast_Score=201, Evalue=5e-52,
Organism=Drosophila melanogaster, GI24657054, Length=284, Percent_Identity=38.7323943661972, Blast_Score=187, Evalue=7e-48,
Organism=Drosophila melanogaster, GI45553081, Length=290, Percent_Identity=37.5862068965517, Blast_Score=186, Evalue=2e-47,
Organism=Drosophila melanogaster, GI24662781, Length=291, Percent_Identity=41.2371134020619, Blast_Score=181, Evalue=3e-46,
Organism=Drosophila melanogaster, GI21356425, Length=277, Percent_Identity=36.4620938628159, Blast_Score=178, Evalue=4e-45,
Organism=Drosophila melanogaster, GI24662785, Length=291, Percent_Identity=40.2061855670103, Blast_Score=172, Evalue=2e-43,
Organism=Drosophila melanogaster, GI281366140, Length=257, Percent_Identity=36.9649805447471, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24646155, Length=172, Percent_Identity=34.8837209302326, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: NA

Molecular weight: Translated: 31552; Mature: 31552

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00062 ALDOKETO_REDUCTASE_2 ; PS00063 ALDOKETO_REDUCTASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYVTLNNGLKMPQLGFGVWKVPNEEVVSPVEHALKVGYRSIDTAKVYGNEVGVGEAISN
CCEEEECCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCHHHHC
SGIPREQLFITTKVWNSDHGYDNTLKAFDESLERLGLDYVDLYLIHWPTPEFDQYVETYK
CCCCHHHEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHH
ALETLYKDGKVKAIGVCNFDIEYLERILNECEIVPVLNQVECHPYLQQKELRDFCEKHNI
HHHHHHCCCCEEEEEEECCCHHHHHHHHHHCCEEECCCHHCCCHHHHHHHHHHHHHHCCE
YLESYSPLMNGKEVLQSEELQNLAERKGKTVAQTILRWHLQSGMIVIPKSVTPNRIEENL
EEECCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEECCCCHHHHHCCC
DVFDFELTDTEMQEINELDRNLRSGAVPSEMNVR
CEEEEEECHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MEYVTLNNGLKMPQLGFGVWKVPNEEVVSPVEHALKVGYRSIDTAKVYGNEVGVGEAISN
CCEEEECCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCHHHHC
SGIPREQLFITTKVWNSDHGYDNTLKAFDESLERLGLDYVDLYLIHWPTPEFDQYVETYK
CCCCHHHEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHH
ALETLYKDGKVKAIGVCNFDIEYLERILNECEIVPVLNQVECHPYLQQKELRDFCEKHNI
HHHHHHCCCCEEEEEEECCCHHHHHHHHHHCCEEECCCHHCCCHHHHHHHHHHHHHHCCE
YLESYSPLMNGKEVLQSEELQNLAERKGKTVAQTILRWHLQSGMIVIPKSVTPNRIEENL
EEECCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEECCCCHHHHHCCC
DVFDFELTDTEMQEINELDRNLRSGAVPSEMNVR
CEEEEEECHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2,5-diketo-D-gluconate; NADPH [C]

Specific reaction: 2,5-diketo-D-gluconate + NADPH = 2-keto-L-gulonate + NADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA