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The map label for this gene is lutB

Identifier: 23097826

GI number: 23097826

Start: 395724

End: 397160

Strand: Direct

Name: lutB

Synonym: OB0371

Alternate gene names: 23097826

Gene position: 395724-397160 (Clockwise)

Preceding gene: 23097825

Following gene: 23097827

Centisome position: 10.9

GC content: 41.27

Gene sequence:

>1437_bases
ATGAGCGTAAAAATTGGTGAGATTTCCTATAAAGAGCGAATTGATAAGGGAATTGATAACGACTTTATGCGTCAAGCCGT
TTCTTCGGCACAAGGGAGATTTCGTAAGGGGCGTCTTTCTCAAGCAGAAGAATTAGGAAATTGGGAAGATTGGCGTCAGC
TAGGTTCAGAGATTCGAACCCACACGTTAGAAAACATCGATTATTATCTTCACCAATTGAGTGAACAAGTAGAAAAACGT
GGAGGGAACGTATTTTTCGCACAAACTGCTGAAGAAGCCAATGAATATATCGAAAATGTTGTGAAAAAGAAACAAGCGAA
AAAAGTTGTCAAAACAAAATCCATGGTTACCGAAGAAATCGGATTGAATGAAGCACTAGAAAAATCAGGAGCAGAAGTAG
TTGAATCGGATTTAGGGGAATGGATCCTACAATTAGATGAGGATCCTCCATCACATATTGTTACACCTGCTTTGCATAAA
AACCGTCAACAAATTCGCGAAACATTTGCAGATAAACGTGGATATGATAAAACAGATTCACCAGAAGAACTGGCAGCCTT
TGCCCGTGAGCAACTACGTCAAGATTTTCTAACTGCTGATGTAGGAATAACCGGCTGTAACTTTGCGATTGCAGAATCAG
GAGCAATAACGTTAGTAACCAATGAAGGAAATGCAGAAATGGTTACTTCATTACCAGATACACAGATTACAGTGATGGGT
ATGGAGCGATTAGTACCGACGTGGGAAGACATGGAAGTACTAGTTAGTTTATTAACACGTGCAGCAGTTGGACAAAAACT
AACTAGCTACATTACAGCCTATACCGGAGCTAGATTAGAAGAAGAGATAGATGGGCCGGATGACTACCATCTAGTAATTG
TTGATAATGGCAGATCCAAGATATTAGGAACAGAATTCCAATCTGCGCTACATTGTATTCGTTGCGCTGCTTGTATTAAT
GTATGCCCGGTGTATCGTCATGTAGGTGGTCACGCGTATAACTCTATTTATCCAGGTCCTATCGGTGCCGTATTAACACC
ACTTCTAGATGGATATGACGATCATAAGGAACTGCCGTATGCTTCTTCTCTTTGTGCAGCTTGTACAGAAGCTTGTCCGG
TGAAGATTCCATTGCATGAACAATTAATCCGTCATCGAGAAATTATTGTAGAGAAGGAGAAGAAATCTCCAGTATCTGAG
AAAATTATGATGAAAGGTTTTGCGCAATGGGCTTCGAACCCAGCAGCTTATAAGTTAAGTACCAAGGTGGCCAGAACCGC
ATTAAAACCATGGACGAAAGATGAGTTTGTAGAAAATGGACCAGGGCCTTTAAAAGGCTGGACGGATGTACGTGATTTCC
CTGCTCCTGGTAAACAATCTTTCCGTGCATGGTGGAGTGAACGCGCGAAGGGAGAAAAGCAAAATGGCAATTCATAA

Upstream 100 bases:

>100_bases
GCTTAATGAACATCGGTGGAAGAATGCGTCGTGAAGGAAAAGATGTAAAGGTCGTTCATATTACAGAAATACTAAATACG
CATCGTGAAGGAGGAATAGC

Downstream 100 bases:

>100_bases
TCGAGATAAATTTTTAAGTAATTTAGCTGCAAACCTTGGCCGTCCTCGGAAAACAGAAGGTGTAGAAAGACCGGAATATA
GTGTGCAACCACAATATGAA

Product: iron-sulfur protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MSVKIGEISYKERIDKGIDNDFMRQAVSSAQGRFRKGRLSQAEELGNWEDWRQLGSEIRTHTLENIDYYLHQLSEQVEKR
GGNVFFAQTAEEANEYIENVVKKKQAKKVVKTKSMVTEEIGLNEALEKSGAEVVESDLGEWILQLDEDPPSHIVTPALHK
NRQQIRETFADKRGYDKTDSPEELAAFAREQLRQDFLTADVGITGCNFAIAESGAITLVTNEGNAEMVTSLPDTQITVMG
MERLVPTWEDMEVLVSLLTRAAVGQKLTSYITAYTGARLEEEIDGPDDYHLVIVDNGRSKILGTEFQSALHCIRCAACIN
VCPVYRHVGGHAYNSIYPGPIGAVLTPLLDGYDDHKELPYASSLCAACTEACPVKIPLHEQLIRHREIIVEKEKKSPVSE
KIMMKGFAQWASNPAAYKLSTKVARTALKPWTKDEFVENGPGPLKGWTDVRDFPAPGKQSFRAWWSERAKGEKQNGNS

Sequences:

>Translated_478_residues
MSVKIGEISYKERIDKGIDNDFMRQAVSSAQGRFRKGRLSQAEELGNWEDWRQLGSEIRTHTLENIDYYLHQLSEQVEKR
GGNVFFAQTAEEANEYIENVVKKKQAKKVVKTKSMVTEEIGLNEALEKSGAEVVESDLGEWILQLDEDPPSHIVTPALHK
NRQQIRETFADKRGYDKTDSPEELAAFAREQLRQDFLTADVGITGCNFAIAESGAITLVTNEGNAEMVTSLPDTQITVMG
MERLVPTWEDMEVLVSLLTRAAVGQKLTSYITAYTGARLEEEIDGPDDYHLVIVDNGRSKILGTEFQSALHCIRCAACIN
VCPVYRHVGGHAYNSIYPGPIGAVLTPLLDGYDDHKELPYASSLCAACTEACPVKIPLHEQLIRHREIIVEKEKKSPVSE
KIMMKGFAQWASNPAAYKLSTKVARTALKPWTKDEFVENGPGPLKGWTDVRDFPAPGKQSFRAWWSERAKGEKQNGNS
>Mature_477_residues
SVKIGEISYKERIDKGIDNDFMRQAVSSAQGRFRKGRLSQAEELGNWEDWRQLGSEIRTHTLENIDYYLHQLSEQVEKRG
GNVFFAQTAEEANEYIENVVKKKQAKKVVKTKSMVTEEIGLNEALEKSGAEVVESDLGEWILQLDEDPPSHIVTPALHKN
RQQIRETFADKRGYDKTDSPEELAAFAREQLRQDFLTADVGITGCNFAIAESGAITLVTNEGNAEMVTSLPDTQITVMGM
ERLVPTWEDMEVLVSLLTRAAVGQKLTSYITAYTGARLEEEIDGPDDYHLVIVDNGRSKILGTEFQSALHCIRCAACINV
CPVYRHVGGHAYNSIYPGPIGAVLTPLLDGYDDHKELPYASSLCAACTEACPVKIPLHEQLIRHREIIVEKEKKSPVSEK
IMMKGFAQWASNPAAYKLSTKVARTALKPWTKDEFVENGPGPLKGWTDVRDFPAPGKQSFRAWWSERAKGEKQNGNS

Specific function: Is involved in L-lactate degradation and allows cells to grow with lactate as the sole carbon source. Has probably a role as an electron transporter during oxidation of L-lactate

COG id: COG1139

COG function: function code C; Uncharacterized conserved protein containing a ferredoxin-like domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains

Homologues:

Organism=Escherichia coli, GI1786498, Length=481, Percent_Identity=50.5197505197505, Blast_Score=531, Evalue=1e-152,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LUTB_OCEIH (Q8ET91)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_691292.1
- ProteinModelPortal:   Q8ET91
- GeneID:   1015657
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB0371
- NMPDR:   fig|221109.1.peg.376
- HOGENOM:   HBG297510
- OMA:   DGPEHMY
- BioCyc:   OIHE221109:OB0371-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_02103
- InterPro:   IPR017900
- InterPro:   IPR003741
- InterPro:   IPR004452
- InterPro:   IPR002698
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR022825
- Gene3D:   G3DSA:3.40.50.10420
- Gene3D:   G3DSA:1.10.1060.10
- TIGRFAMs:   TIGR00273

Pfam domain/function: PF02589 DUF162; SSF46548 Helical_ferredxn

EC number: NA

Molecular weight: Translated: 53450; Mature: 53319

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS00198 4FE4S_FER_1; PS51379 4FE4S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVKIGEISYKERIDKGIDNDFMRQAVSSAQGRFRKGRLSQAEELGNWEDWRQLGSEIRT
CCEEECCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHH
HTLENIDYYLHQLSEQVEKRGGNVFFAQTAEEANEYIENVVKKKQAKKVVKTKSMVTEEI
HHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLNEALEKSGAEVVESDLGEWILQLDEDPPSHIVTPALHKNRQQIRETFADKRGYDKTDS
CHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
PEELAAFAREQLRQDFLTADVGITGCNFAIAESGAITLVTNEGNAEMVTSLPDTQITVMG
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEEECCCCCEEEECCCCCEEEEEE
MERLVPTWEDMEVLVSLLTRAAVGQKLTSYITAYTGARLEEEIDGPDDYHLVIVDNGRSK
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCEEEEEEECCCCC
ILGTEFQSALHCIRCAACINVCPVYRHVGGHAYNSIYPGPIGAVLTPLLDGYDDHKELPY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCH
ASSLCAACTEACPVKIPLHEQLIRHREIIVEKEKKSPVSEKIMMKGFAQWASNPAAYKLS
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEHH
TKVARTALKPWTKDEFVENGPGPLKGWTDVRDFPAPGKQSFRAWWSERAKGEKQNGNS
HHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SVKIGEISYKERIDKGIDNDFMRQAVSSAQGRFRKGRLSQAEELGNWEDWRQLGSEIRT
CEEECCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHH
HTLENIDYYLHQLSEQVEKRGGNVFFAQTAEEANEYIENVVKKKQAKKVVKTKSMVTEEI
HHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLNEALEKSGAEVVESDLGEWILQLDEDPPSHIVTPALHKNRQQIRETFADKRGYDKTDS
CHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
PEELAAFAREQLRQDFLTADVGITGCNFAIAESGAITLVTNEGNAEMVTSLPDTQITVMG
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEEECCCCCEEEECCCCCEEEEEE
MERLVPTWEDMEVLVSLLTRAAVGQKLTSYITAYTGARLEEEIDGPDDYHLVIVDNGRSK
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCEEEEEEECCCCC
ILGTEFQSALHCIRCAACINVCPVYRHVGGHAYNSIYPGPIGAVLTPLLDGYDDHKELPY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCH
ASSLCAACTEACPVKIPLHEQLIRHREIIVEKEKKSPVSEKIMMKGFAQWASNPAAYKLS
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEHH
TKVARTALKPWTKDEFVENGPGPLKGWTDVRDFPAPGKQSFRAWWSERAKGEKQNGNS
HHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376