Click here to switch to the map view.

The map label for this gene is gldA [H]

Identifier: 23097778

GI number: 23097778

Start: 349040

End: 350149

Strand: Direct

Name: gldA [H]

Synonym: OB0323

Alternate gene names: 23097778

Gene position: 349040-350149 (Clockwise)

Preceding gene: 23097777

Following gene: 23097779

Centisome position: 9.61

GC content: 37.3

Gene sequence:

>1110_bases
TTGAGTGAACGAATTTTTATAAGTCCAGCAAAATATGTACAAGGAAAGAATGCAATCCAACGAATTGGCGAATACGTTAA
AGATATTGGTAATCAAGCAATTGTTATTGCAGATGAAAATGTATGGGAAATAGCAGCGAATGACGTTGTAAAGAATTTAA
AAGAAGAGAGTATTGAAGTAGAAGAAGTAGTATTTAACGGAGAAGCTTCCAAAGCTGAGATGGAGCGAATTTCTAAGATT
GCAGATGAATCTAATTCTACTGTAGTTGTAGGGGTTGGTGGTGGTAAAACACTAGATACCGCAAAAGCTGTTTCTGATGA
AGTAAAAGGTCAGACGGTGATTGTACCAACAACAGCTTCAACAGATGCTCCAACTAGTGCGCTTTCTGTAGTTTATTCCG
ATGATGGTGTGTTTGAAGGGTATCGTTTTTATGATAAAAACCCAGACTTAGTAATTGTAGATACAAAAATTGTTGCAGGA
GCTCCTAAACGTTTCTTAGCATCAGGAATTGCTGATGCATTAGCCACTTGGGTAGAAGCACGGAGTGTGATTGAAAATAA
AGGTAAAACAATGGCTGGTGGAATAACCACGATAGCAGGACAAGCTATTGCAGAAAAATGTGAAGAAACGCTATTTAACT
ATGCGGAATTGGCATATGAATCAGTGAAAGCAAAAGCAGTAACACCTGCTCTAGAGAATGTTGTTGAAGCAAATACATTA
TTAAGTGGACTTGGATTTGAAAGTGGTGGACTAGGTGCAGCACATGCGATCCATAATGGTTTTACTGCCTTAGAAGGTGA
AATTCATCACTTAACTCATGGTGAGAAAGTTGCTTTTGGTACCTTAGTTCAATTGGCATTAGAGGAGCGCTCAAAAGAAG
AAATCGAACGATATATTAAATTATATATCGCATTAGACCTTCCAGTAACATTAGAAGATATCAAGCTGAAAGATGCAACT
CGTGAAGATATTATGAAAGTTGCAGAAGCAGCGACTGTTGATGAAGAAACAATTCATGAAGCATTTAATGTTACTGCGGA
TGATGTTGCAGATGCTATCTATGCTGCAGATCAATACTCTAGATCGTATAAAGAAAAACATCAGAAGTAA

Upstream 100 bases:

>100_bases
TAAAGATGCACTCTTTAAATTTTATGAATTAAATTTGTATTACATGGGAATATATAAATAGACATGTATTAATAGACATA
GGTATAAAGGAGTGTACACA

Downstream 100 bases:

>100_bases
TAATTATTTAGAAAAGGCATATCAACACTAATAGTTGGTATGCTTTTTTAATGTCATTTATTTTCTTTTTGTATAGTGAA
ACTTTTCTCTTATAAAAACC

Product: glycerol dehydrogenase

Products: NA

Alternate protein names: GDH; GLDH; GlyDH [H]

Number of amino acids: Translated: 369; Mature: 368

Protein sequence:

>369_residues
MSERIFISPAKYVQGKNAIQRIGEYVKDIGNQAIVIADENVWEIAANDVVKNLKEESIEVEEVVFNGEASKAEMERISKI
ADESNSTVVVGVGGGKTLDTAKAVSDEVKGQTVIVPTTASTDAPTSALSVVYSDDGVFEGYRFYDKNPDLVIVDTKIVAG
APKRFLASGIADALATWVEARSVIENKGKTMAGGITTIAGQAIAEKCEETLFNYAELAYESVKAKAVTPALENVVEANTL
LSGLGFESGGLGAAHAIHNGFTALEGEIHHLTHGEKVAFGTLVQLALEERSKEEIERYIKLYIALDLPVTLEDIKLKDAT
REDIMKVAEAATVDEETIHEAFNVTADDVADAIYAADQYSRSYKEKHQK

Sequences:

>Translated_369_residues
MSERIFISPAKYVQGKNAIQRIGEYVKDIGNQAIVIADENVWEIAANDVVKNLKEESIEVEEVVFNGEASKAEMERISKI
ADESNSTVVVGVGGGKTLDTAKAVSDEVKGQTVIVPTTASTDAPTSALSVVYSDDGVFEGYRFYDKNPDLVIVDTKIVAG
APKRFLASGIADALATWVEARSVIENKGKTMAGGITTIAGQAIAEKCEETLFNYAELAYESVKAKAVTPALENVVEANTL
LSGLGFESGGLGAAHAIHNGFTALEGEIHHLTHGEKVAFGTLVQLALEERSKEEIERYIKLYIALDLPVTLEDIKLKDAT
REDIMKVAEAATVDEETIHEAFNVTADDVADAIYAADQYSRSYKEKHQK
>Mature_368_residues
SERIFISPAKYVQGKNAIQRIGEYVKDIGNQAIVIADENVWEIAANDVVKNLKEESIEVEEVVFNGEASKAEMERISKIA
DESNSTVVVGVGGGKTLDTAKAVSDEVKGQTVIVPTTASTDAPTSALSVVYSDDGVFEGYRFYDKNPDLVIVDTKIVAGA
PKRFLASGIADALATWVEARSVIENKGKTMAGGITTIAGQAIAEKCEETLFNYAELAYESVKAKAVTPALENVVEANTLL
SGLGFESGGLGAAHAIHNGFTALEGEIHHLTHGEKVAFGTLVQLALEERSKEEIERYIKLYIALDLPVTLEDIKLKDATR
EDIMKVAEAATVDEETIHEAFNVTADDVADAIYAADQYSRSYKEKHQK

Specific function: Catalyzes the NAD-dependent oxidation of glycerol to dihydroxyacetone (glycerone). Allows microorganisms to utilize glycerol as a source of carbon under anaerobic conditions. Is also able to use various diols as substrates, such as propan-1,2-diol, butan-

COG id: COG0371

COG function: function code C; Glycerol dehydrogenase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI87082352, Length=365, Percent_Identity=49.041095890411, Blast_Score=337, Evalue=5e-94,
Organism=Escherichia coli, GI1786815, Length=341, Percent_Identity=29.3255131964809, Blast_Score=123, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211
- InterPro:   IPR016205 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: =1.1.1.6 [H]

Molecular weight: Translated: 39833; Mature: 39702

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: PS00913 ADH_IRON_1 ; PS00060 ADH_IRON_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSERIFISPAKYVQGKNAIQRIGEYVKDIGNQAIVIADENVWEIAANDVVKNLKEESIEV
CCCCEEECHHHHHCCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHCCCH
EEVVFNGEASKAEMERISKIADESNSTVVVGVGGGKTLDTAKAVSDEVKGQTVIVPTTAS
HHHEECCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHCCCEEEEECCCC
TDAPTSALSVVYSDDGVFEGYRFYDKNPDLVIVDTKIVAGAPKRFLASGIADALATWVEA
CCCCCHHEEEEECCCCCCCCEEEECCCCCEEEEECCEECCCCHHHHHHHHHHHHHHHHHH
RSVIENKGKTMAGGITTIAGQAIAEKCEETLFNYAELAYESVKAKAVTPALENVVEANTL
HHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSGLGFESGGLGAAHAIHNGFTALEGEIHHLTHGEKVAFGTLVQLALEERSKEEIERYIK
HHHCCCCCCCCCHHHHHHCCCHHHCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LYIALDLPVTLEDIKLKDATREDIMKVAEAATVDEETIHEAFNVTADDVADAIYAADQYS
HHEEECCCEEHHHCEECCCCHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHH
RSYKEKHQK
HHHHHHCCC
>Mature Secondary Structure 
SERIFISPAKYVQGKNAIQRIGEYVKDIGNQAIVIADENVWEIAANDVVKNLKEESIEV
CCCEEECHHHHHCCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHCCCH
EEVVFNGEASKAEMERISKIADESNSTVVVGVGGGKTLDTAKAVSDEVKGQTVIVPTTAS
HHHEECCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHCCCEEEEECCCC
TDAPTSALSVVYSDDGVFEGYRFYDKNPDLVIVDTKIVAGAPKRFLASGIADALATWVEA
CCCCCHHEEEEECCCCCCCCEEEECCCCCEEEEECCEECCCCHHHHHHHHHHHHHHHHHH
RSVIENKGKTMAGGITTIAGQAIAEKCEETLFNYAELAYESVKAKAVTPALENVVEANTL
HHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSGLGFESGGLGAAHAIHNGFTALEGEIHHLTHGEKVAFGTLVQLALEERSKEEIERYIK
HHHCCCCCCCCCHHHHHHCCCHHHCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LYIALDLPVTLEDIKLKDATREDIMKVAEAATVDEETIHEAFNVTADDVADAIYAADQYS
HHEEECCCEEHHHCEECCCCHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHH
RSYKEKHQK
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1339360 [H]