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The map label for this gene is folC [H]

Identifier: 229918367

GI number: 229918367

Start: 2620439

End: 2621641

Strand: Direct

Name: folC [H]

Synonym: EAT1b_2652

Alternate gene names: 229918367

Gene position: 2620439-2621641 (Clockwise)

Preceding gene: 229918365

Following gene: 229918368

Centisome position: 87.35

GC content: 51.37

Gene sequence:

>1203_bases
ATGAAGACGAGACAAGATGTATTCGATTGGTTGAGTGGACAGCTTCGCTTCGGGATCAAACCGGGTCTTGAGCGGATGAA
CTGGATGATTGAGCGGTTAGGGTTAGAAGAACGCCCGTCCATCCATGTGGCCGGTACGAACGGAAAAGGGTCGACCGTCG
CGTTTTTACGGGCGATGGCGATGGAGAACGGCTTATCCGTCGGCACATTTACCTCCCCTTATATCATTCAGTTTGAGGAG
CGGATCATGTTGAACGAGGAACCGATTCCGGAAGACGCGCTCGTCGCCGTGGCGAATCGAGTCAAAGCGTGTGCGGACGA
TGCGCCAGAGACGTTGACGGAGTTCGAGCTCTTGACGATGATGGCATGGTGCTATTTTGATGACACACGTCCAGACTTGA
TCATTTGGGAAGTCGGTCTCGGGGGACGACTGGATTCGACCAATGTGCTGACGCATCCACTTGCGACGATTGTGACATCA
ATTGGACATGACCATCAAGGGATTCTCGGCGATACGCTTGAAGAGATTGCGATGGAGAAATACGGCATTATGAAACGCGG
GGTGCCGATGTTCCATAGTATCGGTGAGGAAAAATTGGTTGAGTTGTTGCATCAAAAAGGGGACGACGTAGGTGCTTCCA
TTCATGATGTTCGTCCACTCGTGAAGAAGATTGTGGACGGGGCAGAGACCGTCGTGACGTATGAGGATATGTCATATGTC
GTGCTCGGATTGACAGGACATCATCAAGCATACAACGGGGCGTGCGCCGTGGCGGTTGCGCGTCACCTCGGATGGAGTGA
TGACGCCATCCGAAGAGGGATTGAGCGAGCACAACATCCGGGACGGTATGAGCTCGTCTCCAAACGACCACGTATCATTT
TAGATGGGGCACATAATCAAGAAGGCATCGAGGCGTTAGTATTACAAGCCAAACAAGAGACAGACGTGACGGTGCTGACG
GCGATTTTAGAAGATAAAGACCGTGACACGATGATTCACGCGCTTCAAGAAATCGGAGACGTGTATGAAACGACATTCGA
CTTTCTGCGTGCGAGAACGAGAACGCAGCTAGAGAAAGATGGGGCGCGATTTGTCGAATGGAAAGCGTGGTTACAGAATT
GGATCGAGTCGCCAACGAGCGAAACGCTGATTGTGACCGGGTCGTTGTATTTCATCAGTGAAGTGAGACGTTTCTTAAGC
TAA

Upstream 100 bases:

>100_bases
CTCCTTCTTCGCTTCGAGTATAGCACAGACTGTTTTCCATAACGGCTGTCGTGAATTTTGATAAGATGAAACAGAAGTGT
AAACAGTAGGAGTGTGGAAG

Downstream 100 bases:

>100_bases
AACATTTACCTGATGTCAAAGAAATAGATGTATATTTTTTCAAGTGGGTGGGATAATTAGTAGTGAAGACTAAAAATAGG
GAGGACTATACATTGCGTAA

Product: FolC bifunctional protein

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MKTRQDVFDWLSGQLRFGIKPGLERMNWMIERLGLEERPSIHVAGTNGKGSTVAFLRAMAMENGLSVGTFTSPYIIQFEE
RIMLNEEPIPEDALVAVANRVKACADDAPETLTEFELLTMMAWCYFDDTRPDLIIWEVGLGGRLDSTNVLTHPLATIVTS
IGHDHQGILGDTLEEIAMEKYGIMKRGVPMFHSIGEEKLVELLHQKGDDVGASIHDVRPLVKKIVDGAETVVTYEDMSYV
VLGLTGHHQAYNGACAVAVARHLGWSDDAIRRGIERAQHPGRYELVSKRPRIILDGAHNQEGIEALVLQAKQETDVTVLT
AILEDKDRDTMIHALQEIGDVYETTFDFLRARTRTQLEKDGARFVEWKAWLQNWIESPTSETLIVTGSLYFISEVRRFLS

Sequences:

>Translated_400_residues
MKTRQDVFDWLSGQLRFGIKPGLERMNWMIERLGLEERPSIHVAGTNGKGSTVAFLRAMAMENGLSVGTFTSPYIIQFEE
RIMLNEEPIPEDALVAVANRVKACADDAPETLTEFELLTMMAWCYFDDTRPDLIIWEVGLGGRLDSTNVLTHPLATIVTS
IGHDHQGILGDTLEEIAMEKYGIMKRGVPMFHSIGEEKLVELLHQKGDDVGASIHDVRPLVKKIVDGAETVVTYEDMSYV
VLGLTGHHQAYNGACAVAVARHLGWSDDAIRRGIERAQHPGRYELVSKRPRIILDGAHNQEGIEALVLQAKQETDVTVLT
AILEDKDRDTMIHALQEIGDVYETTFDFLRARTRTQLEKDGARFVEWKAWLQNWIESPTSETLIVTGSLYFISEVRRFLS
>Mature_400_residues
MKTRQDVFDWLSGQLRFGIKPGLERMNWMIERLGLEERPSIHVAGTNGKGSTVAFLRAMAMENGLSVGTFTSPYIIQFEE
RIMLNEEPIPEDALVAVANRVKACADDAPETLTEFELLTMMAWCYFDDTRPDLIIWEVGLGGRLDSTNVLTHPLATIVTS
IGHDHQGILGDTLEEIAMEKYGIMKRGVPMFHSIGEEKLVELLHQKGDDVGASIHDVRPLVKKIVDGAETVVTYEDMSYV
VLGLTGHHQAYNGACAVAVARHLGWSDDAIRRGIERAQHPGRYELVSKRPRIILDGAHNQEGIEALVLQAKQETDVTVLT
AILEDKDRDTMIHALQEIGDVYETTFDFLRARTRTQLEKDGARFVEWKAWLQNWIESPTSETLIVTGSLYFISEVRRFLS

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=324, Percent_Identity=31.4814814814815, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI66932990, Length=324, Percent_Identity=31.4814814814815, Blast_Score=144, Evalue=2e-34,
Organism=Escherichia coli, GI1788654, Length=392, Percent_Identity=31.1224489795918, Blast_Score=145, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17553150, Length=335, Percent_Identity=28.6567164179104, Blast_Score=128, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17553148, Length=335, Percent_Identity=28.6567164179104, Blast_Score=128, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI71984923, Length=335, Percent_Identity=28.6567164179104, Blast_Score=127, Evalue=7e-30,
Organism=Saccharomyces cerevisiae, GI6323760, Length=429, Percent_Identity=27.2727272727273, Blast_Score=142, Evalue=8e-35,
Organism=Saccharomyces cerevisiae, GI6324815, Length=298, Percent_Identity=27.5167785234899, Blast_Score=117, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24641571, Length=298, Percent_Identity=29.1946308724832, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24581568, Length=288, Percent_Identity=26.0416666666667, Blast_Score=101, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 44963; Mature: 44963

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTRQDVFDWLSGQLRFGIKPGLERMNWMIERLGLEERPSIHVAGTNGKGSTVAFLRAMA
CCCHHHHHHHHCCCEEECCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHH
MENGLSVGTFTSPYIIQFEERIMLNEEPIPEDALVAVANRVKACADDAPETLTEFELLTM
HHCCCCCCCCCCCEEEEEHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
MAWCYFDDTRPDLIIWEVGLGGRLDSTNVLTHPLATIVTSIGHDHQGILGDTLEEIAMEK
HHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
YGIMKRGVPMFHSIGEEKLVELLHQKGDDVGASIHDVRPLVKKIVDGAETVVTYEDMSYV
HHHHHCCCHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHEEEEECCCCEE
VLGLTGHHQAYNGACAVAVARHLGWSDDAIRRGIERAQHPGRYELVSKRPRIILDGAHNQ
EEEECCCCHHHCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHCCCCCEEEECCCCH
EGIEALVLQAKQETDVTVLTAILEDKDRDTMIHALQEIGDVYETTFDFLRARTRTQLEKD
HHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GARFVEWKAWLQNWIESPTSETLIVTGSLYFISEVRRFLS
CHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHC
>Mature Secondary Structure
MKTRQDVFDWLSGQLRFGIKPGLERMNWMIERLGLEERPSIHVAGTNGKGSTVAFLRAMA
CCCHHHHHHHHCCCEEECCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHH
MENGLSVGTFTSPYIIQFEERIMLNEEPIPEDALVAVANRVKACADDAPETLTEFELLTM
HHCCCCCCCCCCCEEEEEHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
MAWCYFDDTRPDLIIWEVGLGGRLDSTNVLTHPLATIVTSIGHDHQGILGDTLEEIAMEK
HHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
YGIMKRGVPMFHSIGEEKLVELLHQKGDDVGASIHDVRPLVKKIVDGAETVVTYEDMSYV
HHHHHCCCHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHEEEEECCCCEE
VLGLTGHHQAYNGACAVAVARHLGWSDDAIRRGIERAQHPGRYELVSKRPRIILDGAHNQ
EEEECCCCHHHCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHCCCCCEEEECCCCH
EGIEALVLQAKQETDVTVLTAILEDKDRDTMIHALQEIGDVYETTFDFLRARTRTQLEKD
HHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GARFVEWKAWLQNWIESPTSETLIVTGSLYFISEVRRFLS
CHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]