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The map label for this gene is hemL2

Identifier: 229918361

GI number: 229918361

Start: 2612414

End: 2613718

Strand: Direct

Name: hemL2

Synonym: EAT1b_2646

Alternate gene names: 229918361

Gene position: 2612414-2613718 (Clockwise)

Preceding gene: 229918360

Following gene: 229918362

Centisome position: 87.08

GC content: 55.71

Gene sequence:

>1305_bases
ATGACATATAACCAAACACGCTCACAAGAAGCGTTCGAACAAGCCCGCCCGCTCATGCCAGGCGGTGTCAACAGTCCGGT
CCGTGCCTACAAATCGGTCGGCATGACGCCAATCTTCGCCGAACGCGGTGAAGGCTCACGCGTCTATGACATCGACGGCA
ACGAGTATATCGATTACGTCCTCTCATGGGGACCACTCATCCTCGGTCACCGTGACCCGGTCGTCACGAAAGCGATTCAA
GAACAAGCCGAAAAAGGCTGGACGTTCGGGACGCCGACCGAGCTCGAGACGAAGATGGCAGAGCTCGTCATCGACCGCGT
CCCATCAATCGAGATGGTCCGTATGGTCAACTCGGGTACGGAAGCGACGATGGCGGCACTTCGCCTCGCACGCGGCTACA
CCGGGAAGACAAAGATTTTGAAATTCGAAGGATGTTACCATGGACATGGCGACTCGCTTCTCATCAAAGCCGGTTCTGGT
GTCGCAACGCTCGGTCTCCCGGACTCACCTGGTGTACCGAAAGAACTCGCGAGCCATACGCTCACGGTGCCTTACAATGA
CTTGGACGCAGTCCGCGTCGCGTTTGAGAAATACGGAGACGACCTCGCTGGTGTCATCGTCGAACCGGCCGCAGGAAACA
TGGGCTTCGTGCCACCACAACCAGGATTCCTTGAAGGACTTCGTGAGATCACAGAACAAAACGGTGCGCTTCTTATCTTT
GATGAGGTCATGACGGGCTTCCGTGTCGGATACAACTGTGCCCAAGGGTACTTCGGTGTCACACCGGACCTCACATGTCT
CGGTAAGGTCATCGGCGGCGGACTTCCGGTCGGCGCTTACGGCGGGAAACGTGAAATCATGGAACAGATCGCACCGCAAG
GTCCGATCTATCAAGCAGGGACGCTCTCGGGTAACCCACTTGCGATGATAGCCGGTTACACGACACTCTCGCAACTCAAA
CCGGAGCACTACGAGGAGTTCGAGCGTAAGGCAGACCGTCTCGCGGAAGGCTTCACGCAGGCCGGTGAGAAATATAACAT
CCCGCACGACACGAACCGTGCCGGCTCGATGTTCGGTTTCTTCTTCACGAACGAGAAAGTAACGAACTTCGAGAAAGCGA
AATCGGCGAACCTCGAGATGTTCCGTTCGTACTATCAGAAGATGGCGGCGCGTGGCGTATTCTTGCCACCGTCACAGTTT
GAAGGATTGTTCCTCTCGACGGCTCATACGGATGAGGATATCGAGAAGACGATCGCAGCGGTCGAGGCGACGTTCAAGGA
GCTTCAAGAAGAGTTTTCACTCTAA

Upstream 100 bases:

>100_bases
TCGTCATGGAGACGATGGTCGGATTCAAACGTGCCGGTGCCGATCTCATCGTCACGTATTTCGCAAAAGACGTCTGTCGC
TATTTGGAAGGAGACTACGC

Downstream 100 bases:

>100_bases
TCTTGACTGCTGACGTTTCTGTCGCTACAATTATTTAACAGATATGATGAATGGCATTGATGAGGAGTAGTACGCATCCT
CCATTCCTTTTAGAGACGCG

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA 2; Glutamate-1-semialdehyde aminotransferase 2; GSA-AT 2

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MTYNQTRSQEAFEQARPLMPGGVNSPVRAYKSVGMTPIFAERGEGSRVYDIDGNEYIDYVLSWGPLILGHRDPVVTKAIQ
EQAEKGWTFGTPTELETKMAELVIDRVPSIEMVRMVNSGTEATMAALRLARGYTGKTKILKFEGCYHGHGDSLLIKAGSG
VATLGLPDSPGVPKELASHTLTVPYNDLDAVRVAFEKYGDDLAGVIVEPAAGNMGFVPPQPGFLEGLREITEQNGALLIF
DEVMTGFRVGYNCAQGYFGVTPDLTCLGKVIGGGLPVGAYGGKREIMEQIAPQGPIYQAGTLSGNPLAMIAGYTTLSQLK
PEHYEEFERKADRLAEGFTQAGEKYNIPHDTNRAGSMFGFFFTNEKVTNFEKAKSANLEMFRSYYQKMAARGVFLPPSQF
EGLFLSTAHTDEDIEKTIAAVEATFKELQEEFSL

Sequences:

>Translated_434_residues
MTYNQTRSQEAFEQARPLMPGGVNSPVRAYKSVGMTPIFAERGEGSRVYDIDGNEYIDYVLSWGPLILGHRDPVVTKAIQ
EQAEKGWTFGTPTELETKMAELVIDRVPSIEMVRMVNSGTEATMAALRLARGYTGKTKILKFEGCYHGHGDSLLIKAGSG
VATLGLPDSPGVPKELASHTLTVPYNDLDAVRVAFEKYGDDLAGVIVEPAAGNMGFVPPQPGFLEGLREITEQNGALLIF
DEVMTGFRVGYNCAQGYFGVTPDLTCLGKVIGGGLPVGAYGGKREIMEQIAPQGPIYQAGTLSGNPLAMIAGYTTLSQLK
PEHYEEFERKADRLAEGFTQAGEKYNIPHDTNRAGSMFGFFFTNEKVTNFEKAKSANLEMFRSYYQKMAARGVFLPPSQF
EGLFLSTAHTDEDIEKTIAAVEATFKELQEEFSL
>Mature_433_residues
TYNQTRSQEAFEQARPLMPGGVNSPVRAYKSVGMTPIFAERGEGSRVYDIDGNEYIDYVLSWGPLILGHRDPVVTKAIQE
QAEKGWTFGTPTELETKMAELVIDRVPSIEMVRMVNSGTEATMAALRLARGYTGKTKILKFEGCYHGHGDSLLIKAGSGV
ATLGLPDSPGVPKELASHTLTVPYNDLDAVRVAFEKYGDDLAGVIVEPAAGNMGFVPPQPGFLEGLREITEQNGALLIFD
EVMTGFRVGYNCAQGYFGVTPDLTCLGKVIGGGLPVGAYGGKREIMEQIAPQGPIYQAGTLSGNPLAMIAGYTTLSQLKP
EHYEEFERKADRLAEGFTQAGEKYNIPHDTNRAGSMFGFFFTNEKVTNFEKAKSANLEMFRSYYQKMAARGVFLPPSQFE
GLFLSTAHTDEDIEKTIAAVEATFKELQEEFSL

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily

Homologues:

Organism=Homo sapiens, GI4557809, Length=331, Percent_Identity=31.7220543806647, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI13994255, Length=373, Percent_Identity=26.0053619302949, Blast_Score=109, Evalue=6e-24,
Organism=Homo sapiens, GI284507298, Length=250, Percent_Identity=34, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI24119277, Length=425, Percent_Identity=23.5294117647059, Blast_Score=93, Evalue=6e-19,
Organism=Homo sapiens, GI37574042, Length=380, Percent_Identity=25, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI226442709, Length=349, Percent_Identity=25.214899713467, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI226442705, Length=380, Percent_Identity=24.4736842105263, Blast_Score=79, Evalue=9e-15,
Organism=Escherichia coli, GI1786349, Length=426, Percent_Identity=56.1032863849765, Blast_Score=512, Evalue=1e-146,
Organism=Escherichia coli, GI145693181, Length=356, Percent_Identity=32.8651685393258, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI1789759, Length=341, Percent_Identity=31.9648093841642, Blast_Score=151, Evalue=9e-38,
Organism=Escherichia coli, GI1788044, Length=343, Percent_Identity=32.9446064139942, Blast_Score=148, Evalue=6e-37,
Organism=Escherichia coli, GI1789016, Length=358, Percent_Identity=29.3296089385475, Blast_Score=142, Evalue=6e-35,
Organism=Escherichia coli, GI1787560, Length=376, Percent_Identity=27.3936170212766, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI1786991, Length=327, Percent_Identity=26.9113149847095, Blast_Score=85, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI25144271, Length=295, Percent_Identity=29.8305084745763, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI71992977, Length=366, Percent_Identity=27.0491803278689, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI32564660, Length=351, Percent_Identity=27.9202279202279, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI25144274, Length=147, Percent_Identity=36.734693877551, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324432, Length=362, Percent_Identity=26.7955801104972, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6323470, Length=319, Percent_Identity=28.5266457680251, Blast_Score=97, Evalue=4e-21,
Organism=Drosophila melanogaster, GI21357415, Length=303, Percent_Identity=27.3927392739274, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSA2_EXISA (C4L4J9)

Other databases:

- EMBL:   CP001615
- RefSeq:   YP_002887007.1
- ProteinModelPortal:   C4L4J9
- GeneID:   7869659
- GenomeReviews:   CP001615_GR
- KEGG:   eat:EAT1b_2646
- OMA:   VGCFGGK
- ProtClustDB:   PRK00062
- GO:   GO:0005737
- HAMAP:   MF_00375
- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11986
- TIGRFAMs:   TIGR00713

Pfam domain/function: PF00202 Aminotran_3; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =5.4.3.8

Molecular weight: Translated: 47444; Mature: 47313

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYNQTRSQEAFEQARPLMPGGVNSPVRAYKSVGMTPIFAERGEGSRVYDIDGNEYIDYV
CCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCEEEEECCCCCEEEECCCCHHHHHH
LSWGPLILGHRDPVVTKAIQEQAEKGWTFGTPTELETKMAELVIDRVPSIEMVRMVNSGT
HHCCCEEECCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCH
EATMAALRLARGYTGKTKILKFEGCYHGHGDSLLIKAGSGVATLGLPDSPGVPKELASHT
HHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEECCCCEEEECCCCCCCCCHHHHCCE
LTVPYNDLDAVRVAFEKYGDDLAGVIVEPAAGNMGFVPPQPGFLEGLREITEQNGALLIF
EECCCCCHHHHHHHHHHHCCCEECEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEE
DEVMTGFRVGYNCAQGYFGVTPDLTCLGKVIGGGLPVGAYGGKREIMEQIAPQGPIYQAG
HHHHHHHHHCHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECC
TLSGNPLAMIAGYTTLSQLKPEHYEEFERKADRLAEGFTQAGEKYNIPHDTNRAGSMFGF
CCCCCCEEEEECHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEE
FFTNEKVTNFEKAKSANLEMFRSYYQKMAARGVFLPPSQFEGLFLSTAHTDEDIEKTIAA
EEECCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHHH
VEATFKELQEEFSL
HHHHHHHHHHHHCC
>Mature Secondary Structure 
TYNQTRSQEAFEQARPLMPGGVNSPVRAYKSVGMTPIFAERGEGSRVYDIDGNEYIDYV
CCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCEEEEECCCCCEEEECCCCHHHHHH
LSWGPLILGHRDPVVTKAIQEQAEKGWTFGTPTELETKMAELVIDRVPSIEMVRMVNSGT
HHCCCEEECCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCH
EATMAALRLARGYTGKTKILKFEGCYHGHGDSLLIKAGSGVATLGLPDSPGVPKELASHT
HHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEECCCCEEEECCCCCCCCCHHHHCCE
LTVPYNDLDAVRVAFEKYGDDLAGVIVEPAAGNMGFVPPQPGFLEGLREITEQNGALLIF
EECCCCCHHHHHHHHHHHCCCEECEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEE
DEVMTGFRVGYNCAQGYFGVTPDLTCLGKVIGGGLPVGAYGGKREIMEQIAPQGPIYQAG
HHHHHHHHHCHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECC
TLSGNPLAMIAGYTTLSQLKPEHYEEFERKADRLAEGFTQAGEKYNIPHDTNRAGSMFGF
CCCCCCEEEEECHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEE
FFTNEKVTNFEKAKSANLEMFRSYYQKMAARGVFLPPSQFEGLFLSTAHTDEDIEKTIAA
EEECCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHHH
VEATFKELQEEFSL
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA