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The map label for this gene is amtB [C]

Identifier: 229918181

GI number: 229918181

Start: 2416054

End: 2417352

Strand: Reverse

Name: amtB [C]

Synonym: EAT1b_2461

Alternate gene names: 229918181

Gene position: 2417352-2416054 (Counterclockwise)

Preceding gene: 229918199

Following gene: 229918178

Centisome position: 80.58

GC content: 53.5

Gene sequence:

>1299_bases
ATGGAAGACATTCTCTATTATATGGACACGCTGTATGTACTCATCGCTGCATTGCTCGTATTGACGATGCAATTAGGTTT
CGGATTACTCGAGACAGGGACGCTTCGGGCGAAGAACGCCGGTCACGTCGCGGTCAAACAAATTATCAGCCTCTCTGTGG
CGGCACTCGCTTTTTGGGCGGTCGGGTTTGGCCTCTCGTTCGGTGACGGGACGACTTGGTTTGGGACAGAAGGATTCTTC
TTAAGCGGATCGTTCACAAGCCTCGACTGGGCGAACGTCTCAATCGACATCAAATTCTTATTCCAGTTATCATTCGTTGC
AGTTTCTCTCGCCATCGCGTGGGGTGGATTTGCAGAACGGGCTAAGCTTTCTGCCTACGTTCTGTTCGGTATCTTCTTCG
TTGCCATCCTCTATCCAATCGTCGCTCGCTCAGTATGGGCTGGTGGAATGCTCGGATCAATGGGGATGCAAGACTTCGCA
GGTTCGGCGGTCGTTCACCTTCAAGGTGGGATGGCGGCTCTCATGGCAACAATCATTTTGAAACCACGTCGTAAAACGGA
ATCACTCGCTGGTCATAACCATGTGTTAACAGTCGTGGGTGGTCTCGTCCTTTGGCTTTGCTGGTTCGGCTTTAACGCCG
GTTCGACAATGTCGGTCGCTGACGGATTCTTCGGTTACGTCGCATTGACAACGATGCTCGCCACAGCAGCTGGTGCGCTC
GGTGCTCTGGCCATCGTCATGCTCCATCGGAAGACGGCAGATATCCCGACAATCGTCAACGGAGTACTCGGGGCGCTCGT
CGCTATCACGGCAGCATGTGCGTTCGTCGAACCGTGGGCAGCCGTCGTCATTGGGGCACTCGCATCGGTCGCAACATATG
GAACGAGCATCTTGATGGCGAAGTATGTGGATGACCCGCTCTGCGCCTTCTCCGTACACGGAGTCGCTGGAATCATCGGA
ACACTCGCACTCGGCTTCTTCGCATCTCCTCGACTCGTTGAGATCACAGGGATCGGTTCTGCCGGACTCTTCTACGGTGG
CGGATTGACACAGCTCGGTGTACAGACACTCGGTGTGTTGATCGCGATGGTCATCGCCGGCGGTGGAAGTTACGCCTTCT
TGAAACTTCTCGATGTCACAATCGGACTCCGTGTCACAGAAGAACAAGAAGATGTCGGCCTCGACATCGCAGAACATGGT
GTATCTGCATACGGTGAAGTCGAAGAAACAACTGAGAAACCAGGACGCCTCCCGAAGATTCTCGGTTCGAGCGTATCGGT
CATGTCACCTAAAAAATAA

Upstream 100 bases:

>100_bases
CTATGTCAGATAATCTGACAAAATGCAATCCAGCATGCAATTTTCGTTTTACACTGAAGTCAACTTTTGGAAAGCACCTA
AGAAAAAGGAGGGCATGAAC

Downstream 100 bases:

>100_bases
TCTACTCACGTGAGAAGGACATCCTGTGCGGGATGTCCTTCTTTTTATTTCAATCGATTCAAGTAATAGCGTGTGACCAT
GACAGACAAGACCAATCCGA

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MEDILYYMDTLYVLIAALLVLTMQLGFGLLETGTLRAKNAGHVAVKQIISLSVAALAFWAVGFGLSFGDGTTWFGTEGFF
LSGSFTSLDWANVSIDIKFLFQLSFVAVSLAIAWGGFAERAKLSAYVLFGIFFVAILYPIVARSVWAGGMLGSMGMQDFA
GSAVVHLQGGMAALMATIILKPRRKTESLAGHNHVLTVVGGLVLWLCWFGFNAGSTMSVADGFFGYVALTTMLATAAGAL
GALAIVMLHRKTADIPTIVNGVLGALVAITAACAFVEPWAAVVIGALASVATYGTSILMAKYVDDPLCAFSVHGVAGIIG
TLALGFFASPRLVEITGIGSAGLFYGGGLTQLGVQTLGVLIAMVIAGGGSYAFLKLLDVTIGLRVTEEQEDVGLDIAEHG
VSAYGEVEETTEKPGRLPKILGSSVSVMSPKK

Sequences:

>Translated_432_residues
MEDILYYMDTLYVLIAALLVLTMQLGFGLLETGTLRAKNAGHVAVKQIISLSVAALAFWAVGFGLSFGDGTTWFGTEGFF
LSGSFTSLDWANVSIDIKFLFQLSFVAVSLAIAWGGFAERAKLSAYVLFGIFFVAILYPIVARSVWAGGMLGSMGMQDFA
GSAVVHLQGGMAALMATIILKPRRKTESLAGHNHVLTVVGGLVLWLCWFGFNAGSTMSVADGFFGYVALTTMLATAAGAL
GALAIVMLHRKTADIPTIVNGVLGALVAITAACAFVEPWAAVVIGALASVATYGTSILMAKYVDDPLCAFSVHGVAGIIG
TLALGFFASPRLVEITGIGSAGLFYGGGLTQLGVQTLGVLIAMVIAGGGSYAFLKLLDVTIGLRVTEEQEDVGLDIAEHG
VSAYGEVEETTEKPGRLPKILGSSVSVMSPKK
>Mature_432_residues
MEDILYYMDTLYVLIAALLVLTMQLGFGLLETGTLRAKNAGHVAVKQIISLSVAALAFWAVGFGLSFGDGTTWFGTEGFF
LSGSFTSLDWANVSIDIKFLFQLSFVAVSLAIAWGGFAERAKLSAYVLFGIFFVAILYPIVARSVWAGGMLGSMGMQDFA
GSAVVHLQGGMAALMATIILKPRRKTESLAGHNHVLTVVGGLVLWLCWFGFNAGSTMSVADGFFGYVALTTMLATAAGAL
GALAIVMLHRKTADIPTIVNGVLGALVAITAACAFVEPWAAVVIGALASVATYGTSILMAKYVDDPLCAFSVHGVAGIIG
TLALGFFASPRLVEITGIGSAGLFYGGGLTQLGVQTLGVLIAMVIAGGGSYAFLKLLDVTIGLRVTEEQEDVGLDIAEHG
VSAYGEVEETTEKPGRLPKILGSSVSVMSPKK

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=405, Percent_Identity=32.0987654320988, Blast_Score=175, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI32564042, Length=442, Percent_Identity=33.2579185520362, Blast_Score=213, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI133931100, Length=436, Percent_Identity=30.5045871559633, Blast_Score=189, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI17550182, Length=431, Percent_Identity=30.3944315545244, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17550180, Length=429, Percent_Identity=30.0699300699301, Blast_Score=159, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6324187, Length=448, Percent_Identity=29.9107142857143, Blast_Score=120, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6325396, Length=414, Percent_Identity=26.3285024154589, Blast_Score=110, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6321559, Length=419, Percent_Identity=24.8210023866348, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI161078314, Length=408, Percent_Identity=36.2745098039216, Blast_Score=200, Evalue=2e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 45052; Mature: 45052

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEDILYYMDTLYVLIAALLVLTMQLGFGLLETGTLRAKNAGHVAVKQIISLSVAALAFWA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCEECCCCCHHHHHHHHHHHHHHHHHHH
VGFGLSFGDGTTWFGTEGFFLSGSFTSLDWANVSIDIKFLFQLSFVAVSLAIAWGGFAER
HHHCCCCCCCCCEECCCCEEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCHHHH
AKLSAYVLFGIFFVAILYPIVARSVWAGGMLGSMGMQDFAGSAVVHLQGGMAALMATIIL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHCCCEEEEECCCHHHHHHHHHH
KPRRKTESLAGHNHVLTVVGGLVLWLCWFGFNAGSTMSVADGFFGYVALTTMLATAAGAL
CCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
GALAIVMLHRKTADIPTIVNGVLGALVAITAACAFVEPWAAVVIGALASVATYGTSILMA
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KYVDDPLCAFSVHGVAGIIGTLALGFFASPRLVEITGIGSAGLFYGGGLTQLGVQTLGVL
HHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHCCCCHHHHHHHHHHHH
IAMVIAGGGSYAFLKLLDVTIGLRVTEEQEDVGLDIAEHGVSAYGEVEETTEKPGRLPKI
HHHHHHCCCCHHHHHHHHHHHCCEEECCHHHCCCCHHHHCCHHCCCHHHHHCCCCCCHHH
LGSSVSVMSPKK
HCCCCCCCCCCC
>Mature Secondary Structure
MEDILYYMDTLYVLIAALLVLTMQLGFGLLETGTLRAKNAGHVAVKQIISLSVAALAFWA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCEECCCCCHHHHHHHHHHHHHHHHHHH
VGFGLSFGDGTTWFGTEGFFLSGSFTSLDWANVSIDIKFLFQLSFVAVSLAIAWGGFAER
HHHCCCCCCCCCEECCCCEEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCHHHH
AKLSAYVLFGIFFVAILYPIVARSVWAGGMLGSMGMQDFAGSAVVHLQGGMAALMATIIL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHCCCEEEEECCCHHHHHHHHHH
KPRRKTESLAGHNHVLTVVGGLVLWLCWFGFNAGSTMSVADGFFGYVALTTMLATAAGAL
CCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
GALAIVMLHRKTADIPTIVNGVLGALVAITAACAFVEPWAAVVIGALASVATYGTSILMA
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KYVDDPLCAFSVHGVAGIIGTLALGFFASPRLVEITGIGSAGLFYGGGLTQLGVQTLGVL
HHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHCCCCHHHHHHHHHHHH
IAMVIAGGGSYAFLKLLDVTIGLRVTEEQEDVGLDIAEHGVSAYGEVEETTEKPGRLPKI
HHHHHHCCCCHHHHHHHHHHHCCEEECCHHHCCCCHHHHCCHHCCCHHHHHCCCCCCHHH
LGSSVSVMSPKK
HCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]