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The map label for this gene is pbpD [H]

Identifier: 229918152

GI number: 229918152

Start: 2386815

End: 2388605

Strand: Reverse

Name: pbpD [H]

Synonym: EAT1b_2432

Alternate gene names: 229918152

Gene position: 2388605-2386815 (Counterclockwise)

Preceding gene: 229918157

Following gene: 229918149

Centisome position: 79.62

GC content: 49.64

Gene sequence:

>1791_bases
ATGAGAGCTTGGATTGGAACCTTATTTTTTGCTGGACTTTTGGCCGCTTCCTTGTCACTTGCCACTGCATTGAAAGAAGA
GATTGACCAGGCAGATGAAATTCTTTCCTGGGGACAAGAGCTGGTATCGGCACAACCACTCTCGTCAAAGCATGCCGTCA
CACTCGCATACAACGGTGGAGAACAGCAGATTTACTTGACTGGCCGCAGAAATGACGTGTCTCCGAAAGCGTTGCCCGAA
CATATTCAAGATGCGATCACGACCATCGAGGACCGTAAATTCAATGCCCATCCCGGTTATGATTTGAGCGGGATTGCCCG
AGCATTTATCAATAACGCGAACGAGACATCTGTCCAAGGTGGGAGTACAATCACGCAACAGTTGGCACGGAACGTGTATC
TCACACATGAACGGACATATGAACGCAAAGTGAAAGAGTTATTGCTTGCGCGAGCGCTAGAAGAAAATTATAGCAAGAAA
GAAATTTTAACAGCTTACAGCAACGTCATTTTTTTTGGACATAATGTATATGGCATTCAATCTGCAGCCGATACATACTT
TTCAAGACCGCTCGACGACTTGAATTGGAATCAACTCGCTTACTTGTTGACGGTGCCGAATAACCCGTCACTTTATGATC
CGCTCAATCCATCCGACGCCTTTTTCGAGCGAAAGGACCGAATCTTGACGCAACTGTTTGAAGCGGGTGTTCTCTCGAAG
ACTGCCTACGATCAGTATCATGGCCTCAAGCTACAAGCGTCGTTTCGCAGTCAATCGATTCAATATCCGGACTACATGGA
TACTGCCGTCCGCGAAGCGATTCAATTGGTACGGTCACGCTACGACTTAGACGATGCCGAGGCGAGAGATTATTTGTCCA
CGCATGAAGCGACAATCGAGACTTATTTATCAACCGGGAAGCAGAAACAAGCAAAACAAGTGATGAGTCAACTTCCTCCC
GGCCTGAACGGGGGCTATACGGAACTCACACCTCAAGGGAGAATCGTGGCCATGGTCGACTCTGAGATGAAGCAGGTCGG
TGAGTTCAACCGAGCGACCCAGGCGTACCGTCAACCAGGTTCCGCAATCAAACCGATCCTCGTGTTCGCCCCATTCATCG
AAGAGACGGGAGCGACACTAGACACGGTACTCGACGGTCGTCCGACTTGTTACGGTTCTTATTGTCCCGGAAATAGTCAC
GACCGTGTGTTAGGCGATGTCTTATTGAAAGATGCGGTCGCTTTCTCGTATAACACCCCTGCCCTCGCCGCCTTCTCAGC
AATCGATCAGGAACGCGCGTACAAGACGGCAGACATGATGTTCAGTCAATGGGATGAACAGGACGAGAACTTAGCGAGTG
CGCTTGGTGGTCTCACACAAGGCGTCTCCCCAAAAGAACTGGCCGCCGCCTATACCCCGTTCCTAAACAAAGGTGAGTAT
ACATCCGGACATACAATCAAACAAATCACATTCAAGTCTGGTGAACCAACGTTGACTCCGATTTTAACGGAAGAGACAGT
CTGGTCTCCGGAAACGATCAGTGAGATGAAAAAAGCGTTATCGACCGTCACCTCATACGGTACAGGTCGCTATGCGAATC
AGCTGAAACGTGAGTTTGAGTATCTCGGAGGAAAGACCGGAACAACGAACGACAATAAAGATATGTGGTATGTCGGACTG
ACCGATCGCACCATCACCGCCATCTGGCTCGGTGCCGATACGCCCCGTCCGTTCCCGGAACTCGCCAACCGTGCGTATCC
GGCGATTGTTTGGACACAGGCACATCAATAA

Upstream 100 bases:

>100_bases
CTTTCCTAAAGTATTCCTTCTCGAATTATTATATGATAACGTTTACATTTTTATTTTTTTGTAAATTGGACGATATAATG
TATAGAGAGAGGTGAAATAG

Downstream 100 bases:

>100_bases
AAGGAGTCAGCCACTGAAAAGTGGACTGACTCCTTTGCTTATGTTCGACGTTCAATCTGAAGTCGATAGGCTCCCCAATG
CTCCTTTAACTCGTCGTGAT

Product: glycosyl transferase family 51

Products: NA

Alternate protein names: PBP 4 [H]

Number of amino acids: Translated: 596; Mature: 596

Protein sequence:

>596_residues
MRAWIGTLFFAGLLAASLSLATALKEEIDQADEILSWGQELVSAQPLSSKHAVTLAYNGGEQQIYLTGRRNDVSPKALPE
HIQDAITTIEDRKFNAHPGYDLSGIARAFINNANETSVQGGSTITQQLARNVYLTHERTYERKVKELLLARALEENYSKK
EILTAYSNVIFFGHNVYGIQSAADTYFSRPLDDLNWNQLAYLLTVPNNPSLYDPLNPSDAFFERKDRILTQLFEAGVLSK
TAYDQYHGLKLQASFRSQSIQYPDYMDTAVREAIQLVRSRYDLDDAEARDYLSTHEATIETYLSTGKQKQAKQVMSQLPP
GLNGGYTELTPQGRIVAMVDSEMKQVGEFNRATQAYRQPGSAIKPILVFAPFIEETGATLDTVLDGRPTCYGSYCPGNSH
DRVLGDVLLKDAVAFSYNTPALAAFSAIDQERAYKTADMMFSQWDEQDENLASALGGLTQGVSPKELAAAYTPFLNKGEY
TSGHTIKQITFKSGEPTLTPILTEETVWSPETISEMKKALSTVTSYGTGRYANQLKREFEYLGGKTGTTNDNKDMWYVGL
TDRTITAIWLGADTPRPFPELANRAYPAIVWTQAHQ

Sequences:

>Translated_596_residues
MRAWIGTLFFAGLLAASLSLATALKEEIDQADEILSWGQELVSAQPLSSKHAVTLAYNGGEQQIYLTGRRNDVSPKALPE
HIQDAITTIEDRKFNAHPGYDLSGIARAFINNANETSVQGGSTITQQLARNVYLTHERTYERKVKELLLARALEENYSKK
EILTAYSNVIFFGHNVYGIQSAADTYFSRPLDDLNWNQLAYLLTVPNNPSLYDPLNPSDAFFERKDRILTQLFEAGVLSK
TAYDQYHGLKLQASFRSQSIQYPDYMDTAVREAIQLVRSRYDLDDAEARDYLSTHEATIETYLSTGKQKQAKQVMSQLPP
GLNGGYTELTPQGRIVAMVDSEMKQVGEFNRATQAYRQPGSAIKPILVFAPFIEETGATLDTVLDGRPTCYGSYCPGNSH
DRVLGDVLLKDAVAFSYNTPALAAFSAIDQERAYKTADMMFSQWDEQDENLASALGGLTQGVSPKELAAAYTPFLNKGEY
TSGHTIKQITFKSGEPTLTPILTEETVWSPETISEMKKALSTVTSYGTGRYANQLKREFEYLGGKTGTTNDNKDMWYVGL
TDRTITAIWLGADTPRPFPELANRAYPAIVWTQAHQ
>Mature_596_residues
MRAWIGTLFFAGLLAASLSLATALKEEIDQADEILSWGQELVSAQPLSSKHAVTLAYNGGEQQIYLTGRRNDVSPKALPE
HIQDAITTIEDRKFNAHPGYDLSGIARAFINNANETSVQGGSTITQQLARNVYLTHERTYERKVKELLLARALEENYSKK
EILTAYSNVIFFGHNVYGIQSAADTYFSRPLDDLNWNQLAYLLTVPNNPSLYDPLNPSDAFFERKDRILTQLFEAGVLSK
TAYDQYHGLKLQASFRSQSIQYPDYMDTAVREAIQLVRSRYDLDDAEARDYLSTHEATIETYLSTGKQKQAKQVMSQLPP
GLNGGYTELTPQGRIVAMVDSEMKQVGEFNRATQAYRQPGSAIKPILVFAPFIEETGATLDTVLDGRPTCYGSYCPGNSH
DRVLGDVLLKDAVAFSYNTPALAAFSAIDQERAYKTADMMFSQWDEQDENLASALGGLTQGVSPKELAAAYTPFLNKGEY
TSGHTIKQITFKSGEPTLTPILTEETVWSPETISEMKKALSTVTSYGTGRYANQLKREFEYLGGKTGTTNDNKDMWYVGL
TDRTITAIWLGADTPRPFPELANRAYPAIVWTQAHQ

Specific function: Cell Wall Formation. Synthesis Of Cross-Linked Peptidoglycan From The Lipid Intermediates. The Enzyme Has A Penicillin-Insensitive Transglycosylase N-Terminal Domain (Formation Of Linear Glycan Strands) And A Penicillin-Sensitive Transpeptidase C-Terminal

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=543, Percent_Identity=27.255985267035, Blast_Score=156, Evalue=4e-39,
Organism=Escherichia coli, GI87082258, Length=216, Percent_Identity=34.7222222222222, Blast_Score=132, Evalue=6e-32,
Organism=Escherichia coli, GI1788867, Length=516, Percent_Identity=26.5503875968992, Blast_Score=130, Evalue=3e-31,
Organism=Escherichia coli, GI1789601, Length=153, Percent_Identity=37.9084967320261, Blast_Score=107, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 66332; Mature: 66332

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAWIGTLFFAGLLAASLSLATALKEEIDQADEILSWGQELVSAQPLSSKHAVTLAYNGG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECC
EQQIYLTGRRNDVSPKALPEHIQDAITTIEDRKFNAHPGYDLSGIARAFINNANETSVQG
CEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCC
GSTITQQLARNVYLTHERTYERKVKELLLARALEENYSKKEILTAYSNVIFFGHNVYGIQ
CHHHHHHHHHCEEECCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCEEEECCCEEECH
SAADTYFSRPLDDLNWNQLAYLLTVPNNPSLYDPLNPSDAFFERKDRILTQLFEAGVLSK
HHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
TAYDQYHGLKLQASFRSQSIQYPDYMDTAVREAIQLVRSRYDLDDAEARDYLSTHEATIE
HHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
TYLSTGKQKQAKQVMSQLPPGLNGGYTELTPQGRIVAMVDSEMKQVGEFNRATQAYRQPG
HHHHCCCHHHHHHHHHHCCCCCCCCCCEECCCCCEEEEECHHHHHHHHHHHHHHHHHCCC
SAIKPILVFAPFIEETGATLDTVLDGRPTCYGSYCPGNSHDRVLGDVLLKDAVAFSYNTP
CCCCHHHHEEHHHHHHCCHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCC
ALAAFSAIDQERAYKTADMMFSQWDEQDENLASALGGLTQGVSPKELAAAYTPFLNKGEY
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCC
TSGHTIKQITFKSGEPTLTPILTEETVWSPETISEMKKALSTVTSYGTGRYANQLKREFE
CCCCEEEEEEECCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
YLGGKTGTTNDNKDMWYVGLTDRTITAIWLGADTPRPFPELANRAYPAIVWTQAHQ
HHCCCCCCCCCCCCEEEEEECCCEEEEEEECCCCCCCCHHHHCCCCCEEEEECCCC
>Mature Secondary Structure
MRAWIGTLFFAGLLAASLSLATALKEEIDQADEILSWGQELVSAQPLSSKHAVTLAYNGG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECC
EQQIYLTGRRNDVSPKALPEHIQDAITTIEDRKFNAHPGYDLSGIARAFINNANETSVQG
CEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCC
GSTITQQLARNVYLTHERTYERKVKELLLARALEENYSKKEILTAYSNVIFFGHNVYGIQ
CHHHHHHHHHCEEECCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCEEEECCCEEECH
SAADTYFSRPLDDLNWNQLAYLLTVPNNPSLYDPLNPSDAFFERKDRILTQLFEAGVLSK
HHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
TAYDQYHGLKLQASFRSQSIQYPDYMDTAVREAIQLVRSRYDLDDAEARDYLSTHEATIE
HHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
TYLSTGKQKQAKQVMSQLPPGLNGGYTELTPQGRIVAMVDSEMKQVGEFNRATQAYRQPG
HHHHCCCHHHHHHHHHHCCCCCCCCCCEECCCCCEEEEECHHHHHHHHHHHHHHHHHCCC
SAIKPILVFAPFIEETGATLDTVLDGRPTCYGSYCPGNSHDRVLGDVLLKDAVAFSYNTP
CCCCHHHHEEHHHHHHCCHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCC
ALAAFSAIDQERAYKTADMMFSQWDEQDENLASALGGLTQGVSPKELAAAYTPFLNKGEY
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCC
TSGHTIKQITFKSGEPTLTPILTEETVWSPETISEMKKALSTVTSYGTGRYANQLKREFE
CCCCEEEEEEECCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
YLGGKTGTTNDNKDMWYVGLTDRTITAIWLGADTPRPFPELANRAYPAIVWTQAHQ
HHCCCCCCCCCCCCEEEEEECCCEEEEEEECCCCCCCCHHHHCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7961491; 9274030; 9384377 [H]