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The map label for this gene is hemL1

Identifier: 229918140

GI number: 229918140

Start: 2370770

End: 2372068

Strand: Reverse

Name: hemL1

Synonym: EAT1b_2420

Alternate gene names: 229918140

Gene position: 2372068-2370770 (Counterclockwise)

Preceding gene: 229918142

Following gene: 229918139

Centisome position: 79.07

GC content: 50.96

Gene sequence:

>1299_bases
ATGACTACTTCATCAAATCGTCAGTCATCCGATGCATTATACCAAGATGCACTCACTCACATCGTCGGAGGTGTCAACAG
TCCATCTCGCTCATTTAAAGCGGTCGGTGGCGGCGCCCCTGTTTATATGGAACGCGGCGAAGGGGCCTATTTTTATGATG
TAGACGGCAATCGTTACATCGATTATTTGGCGGCATACGGTCCAATCATTACCGGCCATGCCCATCCACACATTCACGAA
GCGCTTATGAACGCTTCTTCAAAAGGTTTATTATACGGGACGCCACACCGTCTCGAGGTTGAATTTGCGAAGAAACTAAA
ACAAGCCATCCCTTCGATGGATAAGGTACGATTCACCAACTCCGGAACAGAAGCCGTCATGACGACGGTACGAGTCGCCC
GTGCTTACACTGGTCGTGAACTCGTTGTGAAATTCAGCGGTTGTTACCACGGACATTCGGATCTCATGCTGATTGCAGCA
GGTAGTGGTCCAGCGACGCTCGGTTCTCCTGACTCGGCAGGTGTGACGAAAGCCACTGCCAAAGAAGTCATTACCGTCCC
GTTTAACGACGTCGAAGCGTATCGCGAGATGATGAAAGAATGGGGAGACCAGGTCGCTTGTGTGCTCGTCGAACCGATTG
TCGGGAACTTCGGAATCGTTGAACCGAAGCCAGGTTTCCTCCAAGCTGTCAACGACATCACACATGAACACGGCGCACTC
GTCATCTATGATGAAGTCATCACGGCATTCCGCTTCACATACGGAAGTGCCCAACAAGTGTATGGCATCGAACCAGATAT
GACGGCACTTGGTAAAATCATCGGTGGTGGTCTACCAATCGGTGCTTACGGCGGGAAGGCTGAAATCATGGAGACGGTCG
CCCCACTTGGACCTGCATATCAAGCCGGTACAATGGCCGGTAACCCGGCCTCGATGGCGACAGGAATCGCTTGCCTAGAA
GTGCTCGAGCAACCTGGTGTTTATGAGAAATTAGACGCACTCGGTGCGAAACTGGAAGCCGGCATCCGCGAGGCGGCAGA
AAAGCATGATGTCACGATCACGCTCAACCGCTTAAAAGGTGCCCTTACCGTTTACTTCACGGATGAAATCGTCACCGACT
ATGAAGGTGCGGAAAAGTCAGACGGTGAGAAGTTCGGACGTTTCTTCAAGTTGATGCTTGAGAATGGCGTGAACTTGGCT
CCATCAAAATATGAAGCCTGGTTCTTGACGACAGAGCATACGGAACAAGATATCGAAGAGACAATCGAAGCGGTCAATCG
CTCGTTTGCCCAATTGTAA

Upstream 100 bases:

>100_bases
AAATAGTGTACTGATTTCTCAACTACTCGTCAAACACTGAGCTTATGCGGTTGACGGCTATGATACAATAAGTGCGAATT
TGAATAAGGAGGCAATACAT

Downstream 100 bases:

>100_bases
GAAGGAGATGCGCAAGCATCTCTTTTTTTCGTCTAAAAGGGGACATCTTCAAAAAAAGTGTCTCTTTTTACTTAGTACCG
TCCCCCATTACAGTAGAACC

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA 1; Glutamate-1-semialdehyde aminotransferase 1; GSA-AT 1

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MTTSSNRQSSDALYQDALTHIVGGVNSPSRSFKAVGGGAPVYMERGEGAYFYDVDGNRYIDYLAAYGPIITGHAHPHIHE
ALMNASSKGLLYGTPHRLEVEFAKKLKQAIPSMDKVRFTNSGTEAVMTTVRVARAYTGRELVVKFSGCYHGHSDLMLIAA
GSGPATLGSPDSAGVTKATAKEVITVPFNDVEAYREMMKEWGDQVACVLVEPIVGNFGIVEPKPGFLQAVNDITHEHGAL
VIYDEVITAFRFTYGSAQQVYGIEPDMTALGKIIGGGLPIGAYGGKAEIMETVAPLGPAYQAGTMAGNPASMATGIACLE
VLEQPGVYEKLDALGAKLEAGIREAAEKHDVTITLNRLKGALTVYFTDEIVTDYEGAEKSDGEKFGRFFKLMLENGVNLA
PSKYEAWFLTTEHTEQDIEETIEAVNRSFAQL

Sequences:

>Translated_432_residues
MTTSSNRQSSDALYQDALTHIVGGVNSPSRSFKAVGGGAPVYMERGEGAYFYDVDGNRYIDYLAAYGPIITGHAHPHIHE
ALMNASSKGLLYGTPHRLEVEFAKKLKQAIPSMDKVRFTNSGTEAVMTTVRVARAYTGRELVVKFSGCYHGHSDLMLIAA
GSGPATLGSPDSAGVTKATAKEVITVPFNDVEAYREMMKEWGDQVACVLVEPIVGNFGIVEPKPGFLQAVNDITHEHGAL
VIYDEVITAFRFTYGSAQQVYGIEPDMTALGKIIGGGLPIGAYGGKAEIMETVAPLGPAYQAGTMAGNPASMATGIACLE
VLEQPGVYEKLDALGAKLEAGIREAAEKHDVTITLNRLKGALTVYFTDEIVTDYEGAEKSDGEKFGRFFKLMLENGVNLA
PSKYEAWFLTTEHTEQDIEETIEAVNRSFAQL
>Mature_431_residues
TTSSNRQSSDALYQDALTHIVGGVNSPSRSFKAVGGGAPVYMERGEGAYFYDVDGNRYIDYLAAYGPIITGHAHPHIHEA
LMNASSKGLLYGTPHRLEVEFAKKLKQAIPSMDKVRFTNSGTEAVMTTVRVARAYTGRELVVKFSGCYHGHSDLMLIAAG
SGPATLGSPDSAGVTKATAKEVITVPFNDVEAYREMMKEWGDQVACVLVEPIVGNFGIVEPKPGFLQAVNDITHEHGALV
IYDEVITAFRFTYGSAQQVYGIEPDMTALGKIIGGGLPIGAYGGKAEIMETVAPLGPAYQAGTMAGNPASMATGIACLEV
LEQPGVYEKLDALGAKLEAGIREAAEKHDVTITLNRLKGALTVYFTDEIVTDYEGAEKSDGEKFGRFFKLMLENGVNLAP
SKYEAWFLTTEHTEQDIEETIEAVNRSFAQL

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily

Homologues:

Organism=Homo sapiens, GI4557809, Length=406, Percent_Identity=29.3103448275862, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI37574042, Length=352, Percent_Identity=28.125, Blast_Score=108, Evalue=7e-24,
Organism=Homo sapiens, GI13994255, Length=331, Percent_Identity=26.2839879154079, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI24119277, Length=337, Percent_Identity=26.7062314540059, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI226442705, Length=346, Percent_Identity=27.4566473988439, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI226442709, Length=311, Percent_Identity=28.9389067524116, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI284507298, Length=324, Percent_Identity=27.7777777777778, Blast_Score=90, Evalue=4e-18,
Organism=Escherichia coli, GI1786349, Length=426, Percent_Identity=47.6525821596244, Blast_Score=440, Evalue=1e-125,
Organism=Escherichia coli, GI1789016, Length=334, Percent_Identity=30.2395209580838, Blast_Score=148, Evalue=5e-37,
Organism=Escherichia coli, GI145693181, Length=373, Percent_Identity=30.2949061662198, Blast_Score=131, Evalue=9e-32,
Organism=Escherichia coli, GI1788044, Length=316, Percent_Identity=31.9620253164557, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1787560, Length=362, Percent_Identity=29.5580110497238, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1789759, Length=319, Percent_Identity=29.4670846394984, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI1786991, Length=424, Percent_Identity=24.7641509433962, Blast_Score=82, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI25144271, Length=319, Percent_Identity=30.7210031347962, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI71992977, Length=353, Percent_Identity=26.9121813031161, Blast_Score=108, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI32564660, Length=369, Percent_Identity=27.3712737127371, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI25144274, Length=161, Percent_Identity=31.6770186335404, Blast_Score=82, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6323470, Length=412, Percent_Identity=25.4854368932039, Blast_Score=110, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6324432, Length=341, Percent_Identity=24.633431085044, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21357415, Length=352, Percent_Identity=27.5568181818182, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI161085790, Length=351, Percent_Identity=27.9202279202279, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI28574759, Length=351, Percent_Identity=27.9202279202279, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI21356575, Length=326, Percent_Identity=28.2208588957055, Blast_Score=105, Evalue=8e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSA1_EXISA (C4L367)

Other databases:

- EMBL:   CP001615
- RefSeq:   YP_002886786.1
- ProteinModelPortal:   C4L367
- GeneID:   7869977
- GenomeReviews:   CP001615_GR
- KEGG:   eat:EAT1b_2420
- OMA:   VENYEQA
- ProtClustDB:   PRK12389
- GO:   GO:0005737
- HAMAP:   MF_00375
- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11986
- TIGRFAMs:   TIGR00713

Pfam domain/function: PF00202 Aminotran_3; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =5.4.3.8

Molecular weight: Translated: 46541; Mature: 46410

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTSSNRQSSDALYQDALTHIVGGVNSPSRSFKAVGGGAPVYMERGEGAYFYDVDGNRYI
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCEEEECCCCEEEEECCCCHHH
DYLAAYGPIITGHAHPHIHEALMNASSKGLLYGTPHRLEVEFAKKLKQAIPSMDKVRFTN
HHHHHHCCEEECCCCHHHHHHHHCCCCCCCEECCCCCEEHHHHHHHHHHCCCCHHEEECC
SGTEAVMTTVRVARAYTGRELVVKFSGCYHGHSDLMLIAAGSGPATLGSPDSAGVTKATA
CCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHCC
KEVITVPFNDVEAYREMMKEWGDQVACVLVEPIVGNFGIVEPKPGFLQAVNDITHEHGAL
CCEEEECCCHHHHHHHHHHHHCCCEEEEEEECHHCCCCCCCCCCHHHHHHHHHHHHCCCE
VIYDEVITAFRFTYGSAQQVYGIEPDMTALGKIIGGGLPIGAYGGKAEIMETVAPLGPAY
EEHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCC
QAGTMAGNPASMATGIACLEVLEQPGVYEKLDALGAKLEAGIREAAEKHDVTITLNRLKG
CCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCC
ALTVYFTDEIVTDYEGAEKSDGEKFGRFFKLMLENGVNLAPSKYEAWFLTTEHTEQDIEE
EEEEEEEHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHH
TIEAVNRSFAQL
HHHHHHHHHHCC
>Mature Secondary Structure 
TTSSNRQSSDALYQDALTHIVGGVNSPSRSFKAVGGGAPVYMERGEGAYFYDVDGNRYI
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCEEEECCCCEEEEECCCCHHH
DYLAAYGPIITGHAHPHIHEALMNASSKGLLYGTPHRLEVEFAKKLKQAIPSMDKVRFTN
HHHHHHCCEEECCCCHHHHHHHHCCCCCCCEECCCCCEEHHHHHHHHHHCCCCHHEEECC
SGTEAVMTTVRVARAYTGRELVVKFSGCYHGHSDLMLIAAGSGPATLGSPDSAGVTKATA
CCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHCC
KEVITVPFNDVEAYREMMKEWGDQVACVLVEPIVGNFGIVEPKPGFLQAVNDITHEHGAL
CCEEEECCCHHHHHHHHHHHHCCCEEEEEEECHHCCCCCCCCCCHHHHHHHHHHHHCCCE
VIYDEVITAFRFTYGSAQQVYGIEPDMTALGKIIGGGLPIGAYGGKAEIMETVAPLGPAY
EEHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCC
QAGTMAGNPASMATGIACLEVLEQPGVYEKLDALGAKLEAGIREAAEKHDVTITLNRLKG
CCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCC
ALTVYFTDEIVTDYEGAEKSDGEKFGRFFKLMLENGVNLAPSKYEAWFLTTEHTEQDIEE
EEEEEEEHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHH
TIEAVNRSFAQL
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA