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The map label for this gene is psuK [H]

Identifier: 229918100

GI number: 229918100

Start: 2332353

End: 2333279

Strand: Reverse

Name: psuK [H]

Synonym: EAT1b_2380

Alternate gene names: 229918100

Gene position: 2333279-2332353 (Counterclockwise)

Preceding gene: 229918101

Following gene: 229918099

Centisome position: 77.78

GC content: 49.95

Gene sequence:

>927_bases
ATGAATACGATTGCAGTCATCGGCAAGGTGTTTGTCGATATTAAAGGGACTTCCTTCGCACCGCTCCACAAAGATGCGAA
GAATGTCGGCGACATCACTTTCTCCAATGGCGGAACAGGTCGAAACGTTGCTCAAAACTTAGGTGTGCTCGGTAACGATG
TCCGCTTCGTCTCGACCGTCACGAACGACCAAATCGGTGTCGGCGTCCTCGAAGAATTACGTTGCTTGAATGTAAACGTC
GACGGGGTCGACATGCTTGACGATAATGGTATGGGCATGTGGCTCGCCGTAATGGACAACAACGGCGATCTTCAAACATC
TATTTCGAAGCAACCGGACGAAGCCCGTATGGAAGAAGCGATTTTACGTCAAATCGATACGGTCTTCGAAGAGAGCGACG
CGGTCGCCATCGACTTAGACTTATCGGTCAACGTCTTGAACGAGACGATCGAACTATGCCGTGAAATGGATTTACCGCTT
TATGGTGTCTGTGGCCACCTATCCGTCATCGAACGAAACCGTCATTTGCTTCAAGGATTCACAGGATTCATCTGCAGTCG
TGAGGAAGCGGAAATTTTATCCGACATGTCGATCGTCACAGTAGACGATGCCCTTCGTGTCGCGGAAGTACTCGCCATGA
AAGGTGCCCCCCTCACCATCGTCACAATGAGTGAGCTTGGTGCCGTGTATGTCGACCTTCGTACGAACGAACAAGGTCAC
GTCCCGACGACGAAAGTGAAAGTCGCAGACTCCACGGGAGCTGGTGACTCCTTCTTCTCCGCTGTCATTTCTGAACTTAT
GAAACAACACTCGATTGAAGAGGCGCTTCGATTAGGTATGCGTGTCGCAGGAAAAGTCATCGCTTCGCATGAGAACGGAT
TGACGCAAGAGATGTACGCATCATTAGAACAACCTACGCAAGAATAA

Upstream 100 bases:

>100_bases
GGAATCGATGATTGAAACAATATGTCGATTTCGTTATAATTGAAACAATGTGTCTACAGACTGAAACTGATCGAAAGATT
GACTAAAGGTGGTACAAGAT

Downstream 100 bases:

>100_bases
ATAGAACGCATCGGCGAATCGTCGATGCGTTTTGTGCAAAATCACAGGAACGAAAGGAACGATTTCATTGGTTCGAACGA
AATTAAGAAGCGCGATTATT

Product: PfkB domain protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MNTIAVIGKVFVDIKGTSFAPLHKDAKNVGDITFSNGGTGRNVAQNLGVLGNDVRFVSTVTNDQIGVGVLEELRCLNVNV
DGVDMLDDNGMGMWLAVMDNNGDLQTSISKQPDEARMEEAILRQIDTVFEESDAVAIDLDLSVNVLNETIELCREMDLPL
YGVCGHLSVIERNRHLLQGFTGFICSREEAEILSDMSIVTVDDALRVAEVLAMKGAPLTIVTMSELGAVYVDLRTNEQGH
VPTTKVKVADSTGAGDSFFSAVISELMKQHSIEEALRLGMRVAGKVIASHENGLTQEMYASLEQPTQE

Sequences:

>Translated_308_residues
MNTIAVIGKVFVDIKGTSFAPLHKDAKNVGDITFSNGGTGRNVAQNLGVLGNDVRFVSTVTNDQIGVGVLEELRCLNVNV
DGVDMLDDNGMGMWLAVMDNNGDLQTSISKQPDEARMEEAILRQIDTVFEESDAVAIDLDLSVNVLNETIELCREMDLPL
YGVCGHLSVIERNRHLLQGFTGFICSREEAEILSDMSIVTVDDALRVAEVLAMKGAPLTIVTMSELGAVYVDLRTNEQGH
VPTTKVKVADSTGAGDSFFSAVISELMKQHSIEEALRLGMRVAGKVIASHENGLTQEMYASLEQPTQE
>Mature_308_residues
MNTIAVIGKVFVDIKGTSFAPLHKDAKNVGDITFSNGGTGRNVAQNLGVLGNDVRFVSTVTNDQIGVGVLEELRCLNVNV
DGVDMLDDNGMGMWLAVMDNNGDLQTSISKQPDEARMEEAILRQIDTVFEESDAVAIDLDLSVNVLNETIELCREMDLPL
YGVCGHLSVIERNRHLLQGFTGFICSREEAEILSDMSIVTVDDALRVAEVLAMKGAPLTIVTMSELGAVYVDLRTNEQGH
VPTTKVKVADSTGAGDSFFSAVISELMKQHSIEEALRLGMRVAGKVIASHENGLTQEMYASLEQPTQE

Specific function: Catalyzes the phosphorylation of pseudouridine to pseudouridine 5'-phosphate (PsiMP) [H]

COG id: COG0524

COG function: function code G; Sugar kinases, ribokinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family [H]

Homologues:

Organism=Homo sapiens, GI11545855, Length=227, Percent_Identity=25.9911894273128, Blast_Score=69, Evalue=7e-12,
Organism=Escherichia coli, GI1788491, Length=275, Percent_Identity=25.8181818181818, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1788484, Length=284, Percent_Identity=24.6478873239437, Blast_Score=80, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24583886, Length=260, Percent_Identity=26.5384615384615, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011611
- InterPro:   IPR002173 [H]

Pfam domain/function: PF00294 PfkB [H]

EC number: =2.7.1.83 [H]

Molecular weight: Translated: 33374; Mature: 33374

Theoretical pI: Translated: 4.22; Mature: 4.22

Prosite motif: PS00584 PFKB_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTIAVIGKVFVDIKGTSFAPLHKDAKNVGDITFSNGGTGRNVAQNLGVLGNDVRFVSTV
CCHHHEEEEEEEEECCCCCCCCHHHHHCCCCEEECCCCCCHHHHHHCCCCCCCEEEEEEE
TNDQIGVGVLEELRCLNVNVDGVDMLDDNGMGMWLAVMDNNGDLQTSISKQPDEARMEEA
CCCCCCHHHHHHHHEEECCCCCEEEECCCCCEEEEEEEECCCCEEECCCCCCHHHHHHHH
ILRQIDTVFEESDAVAIDLDLSVNVLNETIELCREMDLPLYGVCGHLSVIERNRHLLQGF
HHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
TGFICSREEAEILSDMSIVTVDDALRVAEVLAMKGAPLTIVTMSELGAVYVDLRTNEQGH
HHHHCCCHHHHHHHCCEEEEHHHHHHHHHHHHHCCCCEEEEEHHHCCEEEEEEECCCCCC
VPTTKVKVADSTGAGDSFFSAVISELMKQHSIEEALRLGMRVAGKVIASHENGLTQEMYA
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
SLEQPTQE
HHCCCCCC
>Mature Secondary Structure
MNTIAVIGKVFVDIKGTSFAPLHKDAKNVGDITFSNGGTGRNVAQNLGVLGNDVRFVSTV
CCHHHEEEEEEEEECCCCCCCCHHHHHCCCCEEECCCCCCHHHHHHCCCCCCCEEEEEEE
TNDQIGVGVLEELRCLNVNVDGVDMLDDNGMGMWLAVMDNNGDLQTSISKQPDEARMEEA
CCCCCCHHHHHHHHEEECCCCCEEEECCCCCEEEEEEEECCCCEEECCCCCCHHHHHHHH
ILRQIDTVFEESDAVAIDLDLSVNVLNETIELCREMDLPLYGVCGHLSVIERNRHLLQGF
HHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
TGFICSREEAEILSDMSIVTVDDALRVAEVLAMKGAPLTIVTMSELGAVYVDLRTNEQGH
HHHHCCCHHHHHHHCCEEEEHHHHHHHHHHHHHCCCCEEEEEHHHCCEEEEEEECCCCCC
VPTTKVKVADSTGAGDSFFSAVISELMKQHSIEEALRLGMRVAGKVIASHENGLTQEMYA
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
SLEQPTQE
HHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 3076173 [H]