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The map label for this gene is yfiY [H]

Identifier: 229917828

GI number: 229917828

Start: 2058635

End: 2059567

Strand: Reverse

Name: yfiY [H]

Synonym: EAT1b_2106

Alternate gene names: 229917828

Gene position: 2059567-2058635 (Counterclockwise)

Preceding gene: 229917834

Following gene: 229917827

Centisome position: 68.65

GC content: 52.52

Gene sequence:

>933_bases
ATGAAAAAATGGATGCTCGCGTTACTTACGCTTTGCTTAACCGTCGTCCTCGCTGCTTGCGGCGGGTCAGACGATACAGA
GAACGATACAAGTTCAGAAACGGCGACTCGCTCGATTGAACACGCGATGGGGACAGCGGACGTACCTGAAAACCCGGAAC
GCGTCGTCGTCTTGACGAATGAAGGGACAGAGGCCCTTCTCGCCCTCGGCGTCAAACCAGTCGGAGCCGTCAAATCATGG
AACGGCGACCCGTGGTACCCGCACATTGAGTCCGAGATGACAGACGTCACGGAAGTCGGGACAGAAAGCGAAGTCAATCT
AGAGGCCATCGCAAAATTGAAACCCGATCTCATCATCGGAACGAAACTTCGTCAAGAGAACATCTATGACAAGTTAAACG
CCATCGCACCGACGGTCATGTCAGAGACGCTCAAAGGTGATTGGCAAGAGAACTTCACCCTTTATGCGAACGCACTCAAC
CTAGAAGAGGAAGGCAATCAAGCGTTGGCCGACTACGAACAGCACGTCGAAGATACGAAAGCAGAACTCGGAGACGCCGT
GAATAAAGAACTCTCGGTCGTCCGTTTCCTTCCTGGTGAGTCACGCATCTACTTCAATGACAGCTTCTCCGGCGTCATCC
TTAACGATGTCGGCATCAAACGTCCAGCGTCACAAGATAAAGCGGAATTCGCTGAACCAGTCGCAATGGAACGAATCCCA
GAGATGGCGGGCGACCACGTCGTCTACTTCACGTATGGTGGGGCCGACGGGTCGAAGACAGCCGAAGAATGGCAATCGAA
CCAGTTGTGGAAAGACCTCGATGCTGTTAAAGCAGGGGAAGTGACAGAAGTCAGTGATGACATCTGGAACACGTCGGGTG
GCGTCTTGTCTGCCAACCAAGTGCTCGATGAACTCGTCGACATTCTAAAATAA

Upstream 100 bases:

>100_bases
TACCATAGCAAATATTACGTTCCAGAAAAAGGGGTTGACAGTGAGAATCATTATCATTTATAGTCAGGTAGGAAAATCAC
CTACATAGAGGAGAATGGTA

Downstream 100 bases:

>100_bases
ATCAAAAGCGCGCCAATTGAATTGGTGCGCTTTTTCATACACTCATATGGTAAAATTTACATATCAACTCATAAGGAGCT
TTTTCTATGCAACGTACACT

Product: periplasmic binding protein

Products: ADP; phosphate; iron dicitrate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MKKWMLALLTLCLTVVLAACGGSDDTENDTSSETATRSIEHAMGTADVPENPERVVVLTNEGTEALLALGVKPVGAVKSW
NGDPWYPHIESEMTDVTEVGTESEVNLEAIAKLKPDLIIGTKLRQENIYDKLNAIAPTVMSETLKGDWQENFTLYANALN
LEEEGNQALADYEQHVEDTKAELGDAVNKELSVVRFLPGESRIYFNDSFSGVILNDVGIKRPASQDKAEFAEPVAMERIP
EMAGDHVVYFTYGGADGSKTAEEWQSNQLWKDLDAVKAGEVTEVSDDIWNTSGGVLSANQVLDELVDILK

Sequences:

>Translated_310_residues
MKKWMLALLTLCLTVVLAACGGSDDTENDTSSETATRSIEHAMGTADVPENPERVVVLTNEGTEALLALGVKPVGAVKSW
NGDPWYPHIESEMTDVTEVGTESEVNLEAIAKLKPDLIIGTKLRQENIYDKLNAIAPTVMSETLKGDWQENFTLYANALN
LEEEGNQALADYEQHVEDTKAELGDAVNKELSVVRFLPGESRIYFNDSFSGVILNDVGIKRPASQDKAEFAEPVAMERIP
EMAGDHVVYFTYGGADGSKTAEEWQSNQLWKDLDAVKAGEVTEVSDDIWNTSGGVLSANQVLDELVDILK
>Mature_310_residues
MKKWMLALLTLCLTVVLAACGGSDDTENDTSSETATRSIEHAMGTADVPENPERVVVLTNEGTEALLALGVKPVGAVKSW
NGDPWYPHIESEMTDVTEVGTESEVNLEAIAKLKPDLIIGTKLRQENIYDKLNAIAPTVMSETLKGDWQENFTLYANALN
LEEEGNQALADYEQHVEDTKAELGDAVNKELSVVRFLPGESRIYFNDSFSGVILNDVGIKRPASQDKAEFAEPVAMERIP
EMAGDHVVYFTYGGADGSKTAEEWQSNQLWKDLDAVKAGEVTEVSDDIWNTSGGVLSANQVLDELVDILK

Specific function: Part of the ABC transporter complex yfiYZ/yfhA/yusV involved in import of the iron-hydroxamate siderophores schizokinen, arthrobactin and corprogen. Binds the siderophores and delivers them to the surface of yfiZ/yfhA (Probable) [H]

COG id: COG0614

COG function: function code P; ABC-type Fe3+-hydroxamate transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable). Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe/B12 periplasmic-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87082411, Length=280, Percent_Identity=26.0714285714286, Blast_Score=92, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002491 [H]

Pfam domain/function: PF01497 Peripla_BP_2 [H]

EC number: NA

Molecular weight: Translated: 33882; Mature: 33882

Theoretical pI: Translated: 4.03; Mature: 4.03

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS50983 FE_B12_PBP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKWMLALLTLCLTVVLAACGGSDDTENDTSSETATRSIEHAMGTADVPENPERVVVLTN
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEC
EGTEALLALGVKPVGAVKSWNGDPWYPHIESEMTDVTEVGTESEVNLEAIAKLKPDLIIG
CCCHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHCCCCEEEE
TKLRQENIYDKLNAIAPTVMSETLKGDWQENFTLYANALNLEEEGNQALADYEQHVEDTK
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCHHCCCHHHHHHHHHHHHHH
AELGDAVNKELSVVRFLPGESRIYFNDSFSGVILNDVGIKRPASQDKAEFAEPVAMERIP
HHHHHHHHHHCEEEEECCCCCEEEECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHH
EMAGDHVVYFTYGGADGSKTAEEWQSNQLWKDLDAVKAGEVTEVSDDIWNTSGGVLSANQ
HHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCEEEHHH
VLDELVDILK
HHHHHHHHHC
>Mature Secondary Structure
MKKWMLALLTLCLTVVLAACGGSDDTENDTSSETATRSIEHAMGTADVPENPERVVVLTN
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEC
EGTEALLALGVKPVGAVKSWNGDPWYPHIESEMTDVTEVGTESEVNLEAIAKLKPDLIIG
CCCHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHCCCCEEEE
TKLRQENIYDKLNAIAPTVMSETLKGDWQENFTLYANALNLEEEGNQALADYEQHVEDTK
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCHHCCCHHHHHHHHHHHHHH
AELGDAVNKELSVVRFLPGESRIYFNDSFSGVILNDVGIKRPASQDKAEFAEPVAMERIP
HHHHHHHHHHCEEEEECCCCCEEEECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHH
EMAGDHVVYFTYGGADGSKTAEEWQSNQLWKDLDAVKAGEVTEVSDDIWNTSGGVLSANQ
HHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCEEEHHH
VLDELVDILK
HHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; iron dicitrate [Periplasm]; H2O [C]

Specific reaction: ATP + iron dicitrate [Periplasm] + H2O = ADP + phosphate + iron dicitrate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8946165; 9384377 [H]