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The map label for this gene is pdxK [H]

Identifier: 229917724

GI number: 229917724

Start: 1955267

End: 1956091

Strand: Reverse

Name: pdxK [H]

Synonym: EAT1b_2001

Alternate gene names: 229917724

Gene position: 1956091-1955267 (Counterclockwise)

Preceding gene: 229917726

Following gene: 229917723

Centisome position: 65.21

GC content: 50.79

Gene sequence:

>825_bases
ATGACGATGAAAAAAGCATTGACGATTGCCGGTTCTGACACAAGTGGTGGCGCGGGAATCCAAGCCGACCTCAAAACGAT
GGAAGAACTCGGTGTGTATGGTATGACAGCCTTGACGGTCATCGTGGCACAAGACCCACATAACGCATGGAATCACGAAG
TCTTCCCAATCGACACGACCTTGATTGAGAAGCAAATCGATACGGTGCTCGCTGGAATTGGCGTCGATGCCGTGAAGACA
GGTATGCTTCCGACACCTGAAATCATCGAACTCGCCGCACGGAAAATCAAAGAATACGGCATCCAGAATGCCGTCGTCGA
CCCAGTCATGGCATGTAAAGGTGCGGATGAGATCCTCGACCCAAACGTCGCGGTCGCGATGCGTCAACACCTCGTTCCAG
TCGCAAAAATTATCACACCGAACTTATTCGAGGCGCGTATGCTCGCTGGCCTCGATAAAACACCTGCAACGATGGACGAA
ATCAAAGAAGCCGCTCGTTTAATCCACGAACTCGGCGCAGAGATCGTCATCGTCAAAGTCGGTGGCAAACTCGGATTTGA
TACCGCATTCGACGTCTTGTACGACGGAAACGAATTCCGCCTTCTCGAAAGTGAAAAAATCGAGCCTGCGTTCACACACG
GCGGCGGATGTACGTTCTCGTCTGCCATCGCGGCCTCACTCGCAAACGGTCATACGGTCGAAGAAGCGATTGAAATCGGA
AAAGAATTCATCACAGAAGCTGTTCGTCATTCATTCCGTCTCAACCAATACGTTGGACCGACGAACCATACCGGATACCG
TAAAAAAGTAGCAACACAATCGTAA

Upstream 100 bases:

>100_bases
CTGAAAAGTTATGAACACGTCTCATAATCCTTGCATTTGTCGGAATAGGAAAAAGTCGTTATAGTGAAACTATCCAAACA
GACAAAGGGGATGGACGATT

Downstream 100 bases:

>100_bases
ACAGATGACCCGGAACTACGCGTTTCGGGTCATCCTTTCCATTGGAGGGACAGACGTGAAAGCGATTCAACCTGATCACA
TTCAGCACTATTTAGACAAT

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: PN/PL/PM kinase; Pyridoxal kinase; Pyridoxamine kinase; Vitamin B6 kinase [H]

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MTMKKALTIAGSDTSGGAGIQADLKTMEELGVYGMTALTVIVAQDPHNAWNHEVFPIDTTLIEKQIDTVLAGIGVDAVKT
GMLPTPEIIELAARKIKEYGIQNAVVDPVMACKGADEILDPNVAVAMRQHLVPVAKIITPNLFEARMLAGLDKTPATMDE
IKEAARLIHELGAEIVIVKVGGKLGFDTAFDVLYDGNEFRLLESEKIEPAFTHGGGCTFSSAIAASLANGHTVEEAIEIG
KEFITEAVRHSFRLNQYVGPTNHTGYRKKVATQS

Sequences:

>Translated_274_residues
MTMKKALTIAGSDTSGGAGIQADLKTMEELGVYGMTALTVIVAQDPHNAWNHEVFPIDTTLIEKQIDTVLAGIGVDAVKT
GMLPTPEIIELAARKIKEYGIQNAVVDPVMACKGADEILDPNVAVAMRQHLVPVAKIITPNLFEARMLAGLDKTPATMDE
IKEAARLIHELGAEIVIVKVGGKLGFDTAFDVLYDGNEFRLLESEKIEPAFTHGGGCTFSSAIAASLANGHTVEEAIEIG
KEFITEAVRHSFRLNQYVGPTNHTGYRKKVATQS
>Mature_273_residues
TMKKALTIAGSDTSGGAGIQADLKTMEELGVYGMTALTVIVAQDPHNAWNHEVFPIDTTLIEKQIDTVLAGIGVDAVKTG
MLPTPEIIELAARKIKEYGIQNAVVDPVMACKGADEILDPNVAVAMRQHLVPVAKIITPNLFEARMLAGLDKTPATMDEI
KEAARLIHELGAEIVIVKVGGKLGFDTAFDVLYDGNEFRLLESEKIEPAFTHGGGCTFSSAIAASLANGHTVEEAIEIGK
EFITEAVRHSFRLNQYVGPTNHTGYRKKVATQS

Specific function: Phosphorylates B6 vitamers; functions in a salvage pathway. Uses pyridoxal, pyridoxine, and pyridoxamine as substrates. Can also use hydroxymethylpyrimidine (HMP) as substrate [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=266, Percent_Identity=36.0902255639098, Blast_Score=157, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6325378, Length=269, Percent_Identity=30.4832713754647, Blast_Score=122, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6324997, Length=276, Percent_Identity=31.8840579710145, Blast_Score=104, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6324517, Length=276, Percent_Identity=30.0724637681159, Blast_Score=95, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.35 [H]

Molecular weight: Translated: 29426; Mature: 29295

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMKKALTIAGSDTSGGAGIQADLKTMEELGVYGMTALTVIVAQDPHNAWNHEVFPIDTT
CCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCHHHHHEEEEEECCCCCCCCCEEECCHHH
LIEKQIDTVLAGIGVDAVKTGMLPTPEIIELAARKIKEYGIQNAVVDPVMACKGADEILD
HHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHC
PNVAVAMRQHLVPVAKIITPNLFEARMLAGLDKTPATMDEIKEAARLIHELGAEIVIVKV
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEE
GGKLGFDTAFDVLYDGNEFRLLESEKIEPAFTHGGGCTFSSAIAASLANGHTVEEAIEIG
CCCCCCCHHHHHEECCCEEEEEECCCCCCCEECCCCCHHHHHHHHHHCCCCHHHHHHHHH
KEFITEAVRHSFRLNQYVGPTNHTGYRKKVATQS
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCC
>Mature Secondary Structure 
TMKKALTIAGSDTSGGAGIQADLKTMEELGVYGMTALTVIVAQDPHNAWNHEVFPIDTT
CCCCEEEEECCCCCCCCCCHHHHHHHHHHCCHHHHHEEEEEECCCCCCCCCEEECCHHH
LIEKQIDTVLAGIGVDAVKTGMLPTPEIIELAARKIKEYGIQNAVVDPVMACKGADEILD
HHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHC
PNVAVAMRQHLVPVAKIITPNLFEARMLAGLDKTPATMDEIKEAARLIHELGAEIVIVKV
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEE
GGKLGFDTAFDVLYDGNEFRLLESEKIEPAFTHGGGCTFSSAIAASLANGHTVEEAIEIG
CCCCCCCHHHHHEECCCEEEEEECCCCCCCEECCCCCHHHHHHHHHHCCCCHHHHHHHHH
KEFITEAVRHSFRLNQYVGPTNHTGYRKKVATQS
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]