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The map label for this gene is rlmH

Identifier: 229220841

GI number: 229220841

Start: 5200159

End: 5200638

Strand: Reverse

Name: rlmH

Synonym: BAS5312

Alternate gene names: 229220841

Gene position: 5200638-5200159 (Counterclockwise)

Preceding gene: 49188297

Following gene: 49188295

Centisome position: 99.46

GC content: 32.92

Gene sequence:

>480_bases
GTGAATATCTCGATTATTTCAATTGGGAAATTAAAAGAAAAATATTTAAAACAAGGTATAGCAGAATACTTAAAACGATT
ATCTGCATACGCAAAAGTAGAAGTAATTGAATTGCCAGATGAAAAGGCACCAGAAAATTTAAGTGAAGCAGAAATGCTAA
TTGTAAAAGAAAAAGAAGGTATACGTATACTAGATAAAATTTCTGATGATACGCATGTCATTGCGTTAGCGATAGAAGGA
AAGCAAAAATCATCAGAAGAATTTGCAGTAAGCTTAGATCGTCTTGCTACATATGGAAAAAGTAAAGTTGCCTTCGTGAT
TGGTGGATCACTTGGACTAAGTTCAGAAGTAATGAAGCGTTCAAATGAATCTCTTTCTTTTTCAAAGATGACATTACCAC
ACCAATTAATGCGATTAGTATTGCTTGAGCAAGTGTATAGAGCATTTCGTATTAATCGTGGAGAACCGTATCATAAGTAA

Upstream 100 bases:

>100_bases
ATGTTGTATCGAACACAGATTCTCACACAATATGTGGGTAACAATTGTGGATATGTGGATAAGTTATGTGGATAACATGT
TTGTAAGGTGGGGAATACGT

Downstream 100 bases:

>100_bases
AATTACTTTTTGCATATAAAAAGAGTAAAAAAGACCATTGGACAAAAATCACCGATGGTCTTTTACACATTAATAGGTGT
TATATAGATTTCCTAAAATA

Product: rRNA large subunit methyltransferase

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MNISIISIGKLKEKYLKQGIAEYLKRLSAYAKVEVIELPDEKAPENLSEAEMLIVKEKEGIRILDKISDDTHVIALAIEG
KQKSSEEFAVSLDRLATYGKSKVAFVIGGSLGLSSEVMKRSNESLSFSKMTLPHQLMRLVLLEQVYRAFRINRGEPYHK

Sequences:

>Translated_159_residues
MNISIISIGKLKEKYLKQGIAEYLKRLSAYAKVEVIELPDEKAPENLSEAEMLIVKEKEGIRILDKISDDTHVIALAIEG
KQKSSEEFAVSLDRLATYGKSKVAFVIGGSLGLSSEVMKRSNESLSFSKMTLPHQLMRLVLLEQVYRAFRINRGEPYHK
>Mature_159_residues
MNISIISIGKLKEKYLKQGIAEYLKRLSAYAKVEVIELPDEKAPENLSEAEMLIVKEKEGIRILDKISDDTHVIALAIEG
KQKSSEEFAVSLDRLATYGKSKVAFVIGGSLGLSSEVMKRSNESLSFSKMTLPHQLMRLVLLEQVYRAFRINRGEPYHK

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family

Homologues:

Organism=Escherichia coli, GI1786855, Length=159, Percent_Identity=31.4465408805031, Blast_Score=93, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMH_BACAC (C3LGR6)

Other databases:

- EMBL:   CP001215
- RefSeq:   YP_002818237.1
- ProteinModelPortal:   C3LGR6
- SMR:   C3LGR6
- EnsemblBacteria:   EBBACT00000118800
- GeneID:   7784641
- GenomeReviews:   CP001215_GR
- KEGG:   bah:BAMEG_5756
- GeneTree:   EBGT00050000003035
- ProtClustDB:   PRK00103
- GO:   GO:0005737
- HAMAP:   MF_00658
- InterPro:   IPR016051
- InterPro:   IPR003742
- PIRSF:   PIRSF004505
- TIGRFAMs:   TIGR00246

Pfam domain/function: PF02590 SPOUT_MTase

EC number: NA

Molecular weight: Translated: 17927; Mature: 17927

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNISIISIGKLKEKYLKQGIAEYLKRLSAYAKVEVIELPDEKAPENLSEAEMLIVKEKEG
CCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCEEEEEECCCC
IRILDKISDDTHVIALAIEGKQKSSEEFAVSLDRLATYGKSKVAFVIGGSLGLSSEVMKR
CEEEECCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHC
SNESLSFSKMTLPHQLMRLVLLEQVYRAFRINRGEPYHK
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNISIISIGKLKEKYLKQGIAEYLKRLSAYAKVEVIELPDEKAPENLSEAEMLIVKEKEG
CCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCEEEEEECCCC
IRILDKISDDTHVIALAIEGKQKSSEEFAVSLDRLATYGKSKVAFVIGGSLGLSSEVMKR
CEEEECCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHC
SNESLSFSKMTLPHQLMRLVLLEQVYRAFRINRGEPYHK
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA