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The map label for this gene is btuB [H]

Identifier: 229220642

GI number: 229220642

Start: 4391024

End: 4392919

Strand: Reverse

Name: btuB [H]

Synonym: YPO3910

Alternate gene names: 229220642

Gene position: 4392919-4391024 (Counterclockwise)

Preceding gene: 218930912

Following gene: 218930909

Centisome position: 94.4

GC content: 46.52

Gene sequence:

>1896_bases
ATGATAATTAAAAATACAATGACAATTAAAAAATATACGCTGCTCACAGCCCTTTCTGTGACGGCATTTTCTGGCTGGGC
GCAGGGTAATAACACCACGGACAACAATGATGAAATGGTTGTAACAGCCAACCGTTTTCCACAACCGAAAAGTAGTGTAT
TAGCGCCTGTCGATGTAGTCACTCGTGCCGATATTGATCGCTGGCAATCTACAAATATAAATGATGTCTTACGCCGCTTA
CCGGGTGTTGATATTGCTCAGGATGGCGGTATGGGCCAGCGCAGTTCTTTATTTATCCGGGGCACTAATTCTAGCCATGT
CTTGGTTCTGATCGATGGCGTCCGCCTCAATCAAGCGGGGATTACCGGCGCATCAGATCTCAGTCAAATCCCTATTTCAT
TGGTACAGCGCATCGAATACATTCGTGGTCCACGTTCTGCCGTCTATGGTTCAGATGCCATTGGAGGGGTGATTAATATC
CTGACTGGACGGGATAAACCAGGAACCACACTTTCTGCTGGGTTGGGTTCAAACGGTTATCAAACCTATGATGGTTCCAC
TCAACAGAAACTGGGTGAAGACACGACGGTGACATTGGCGGGAAATTACACTTATAGCAAAGGGTATGATGTGGTTGCTG
GTATGCCGGGAGCAGGTGGACCACGTCAGCCTGACCGGGATGGTTTCATGGGGAAAATGCTGTGGGCGGGCTTAGAACAT
CAGTTTAACGAGCAGTTTAATGGTTTTGCCCGAGTATATGGTTTCGATAACCGCAGTGATTATGACGGGTATACCAACTA
TAGCAATCCATTGGCGTTAATCGATACCCGTAAACTCTCCAGCCGTACCTATGACACGGGACTACGCTACAAGAATGGCA
TTTATGCTTCCCAATTTATTGCCAGTTATAACCGAACTAAAGATTATAATTACAGCCCACTTTTCGGCCAGCATGATATT
ACGGCCTCGTTAGATGAAGCTGAGCAATATAACTTGCAATGGGGTAACACCTTCCAGTTAACGAATGGCATGATCAGTGC
GGGTGCGGATTGGCAAGAGCAAAGAACCGAACGTAAAAGCAGTAACCAGAATACTACGGCCGATTTTACGCAGCACAACA
CGGGCATCTATTTGACAGGCCAACAGCAAATCAGCGACGTTACATTAGAGGGGGCTGTACGCAGTGACGATAACTCGCAG
TTTGGGTGGCACTCAACTTGGCAGACCAGCGCCGGTTGGGAATTTATTGATGGCTACCGCTTGATTGGTTCTTACGGCAC
AGCCTATAAGGCACCTAATCTGATGCAGCTTTATAGCGCTTACGGTGGTAATGCCAATCTGAAACCGGAAGAAAGTAAGC
AATGGGAAGGTGGCGTCGAAGGATTAACGGGGCCGCTGACTTGGCGTCTATCTGCTTATCGTAACGATATTGATCAGCTT
ATCGATTACAGCAATCTGACCAACGGTTATTTCAATATCAATAAAGCCACGATTAAAGGTGTTGAATGGACAGGTTCGTT
TGATACTGGGCCGTTATCACATCAAGTGACGCTGGAATATCTGGACCCACGTAATGCAGATACCCACGAAATTCTGGTGC
GCCGAGCCAAGCAACAAGTAAAATATCAGTTGGATTGGCAGGTAGCTGATTTGGATTGGTCTGTTACCTACCAATATCTT
GGTCAGCGTTATGACAAAGACTACAGCACTTATCCAGAAGAAACGGTTGAACTCGGTGGTGTGAGCCTTTGGGATCTCGC
TGTTTCGTATCCGGTCACATCTCATCTCACGGTTCGTGGTAGAATTGCCAACCTGTTTGATAAAGATTATGAGATGGTTT
ATGGCTACCAAACCCCAGGACGCGAATACTACTTCACGGGAAGCTATAACTTCTAA

Upstream 100 bases:

>100_bases
CCCGAAGACCTGCCGGTATTACGTCGCAATATTCGTGGCCGTCGCGTACTACCGGCCATGGCACTGCGGCATCCGCCAAT
TGAGTTTGGATGCTCTCTTA

Downstream 100 bases:

>100_bases
GGCTGACTCTCCTCCTCGCCCGACAGCACTGATTTTTGATTCGGGTGTTGGCGGGCTGTCTGTCTATCAAGAGATTCGGC
AACTGTTGCCGGATCTCCAC

Product: vitamin B12/cobalamin outer membrane transporter

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 631; Mature: 631

Protein sequence:

>631_residues
MIIKNTMTIKKYTLLTALSVTAFSGWAQGNNTTDNNDEMVVTANRFPQPKSSVLAPVDVVTRADIDRWQSTNINDVLRRL
PGVDIAQDGGMGQRSSLFIRGTNSSHVLVLIDGVRLNQAGITGASDLSQIPISLVQRIEYIRGPRSAVYGSDAIGGVINI
LTGRDKPGTTLSAGLGSNGYQTYDGSTQQKLGEDTTVTLAGNYTYSKGYDVVAGMPGAGGPRQPDRDGFMGKMLWAGLEH
QFNEQFNGFARVYGFDNRSDYDGYTNYSNPLALIDTRKLSSRTYDTGLRYKNGIYASQFIASYNRTKDYNYSPLFGQHDI
TASLDEAEQYNLQWGNTFQLTNGMISAGADWQEQRTERKSSNQNTTADFTQHNTGIYLTGQQQISDVTLEGAVRSDDNSQ
FGWHSTWQTSAGWEFIDGYRLIGSYGTAYKAPNLMQLYSAYGGNANLKPEESKQWEGGVEGLTGPLTWRLSAYRNDIDQL
IDYSNLTNGYFNINKATIKGVEWTGSFDTGPLSHQVTLEYLDPRNADTHEILVRRAKQQVKYQLDWQVADLDWSVTYQYL
GQRYDKDYSTYPEETVELGGVSLWDLAVSYPVTSHLTVRGRIANLFDKDYEMVYGYQTPGREYYFTGSYNF

Sequences:

>Translated_631_residues
MIIKNTMTIKKYTLLTALSVTAFSGWAQGNNTTDNNDEMVVTANRFPQPKSSVLAPVDVVTRADIDRWQSTNINDVLRRL
PGVDIAQDGGMGQRSSLFIRGTNSSHVLVLIDGVRLNQAGITGASDLSQIPISLVQRIEYIRGPRSAVYGSDAIGGVINI
LTGRDKPGTTLSAGLGSNGYQTYDGSTQQKLGEDTTVTLAGNYTYSKGYDVVAGMPGAGGPRQPDRDGFMGKMLWAGLEH
QFNEQFNGFARVYGFDNRSDYDGYTNYSNPLALIDTRKLSSRTYDTGLRYKNGIYASQFIASYNRTKDYNYSPLFGQHDI
TASLDEAEQYNLQWGNTFQLTNGMISAGADWQEQRTERKSSNQNTTADFTQHNTGIYLTGQQQISDVTLEGAVRSDDNSQ
FGWHSTWQTSAGWEFIDGYRLIGSYGTAYKAPNLMQLYSAYGGNANLKPEESKQWEGGVEGLTGPLTWRLSAYRNDIDQL
IDYSNLTNGYFNINKATIKGVEWTGSFDTGPLSHQVTLEYLDPRNADTHEILVRRAKQQVKYQLDWQVADLDWSVTYQYL
GQRYDKDYSTYPEETVELGGVSLWDLAVSYPVTSHLTVRGRIANLFDKDYEMVYGYQTPGREYYFTGSYNF
>Mature_631_residues
MIIKNTMTIKKYTLLTALSVTAFSGWAQGNNTTDNNDEMVVTANRFPQPKSSVLAPVDVVTRADIDRWQSTNINDVLRRL
PGVDIAQDGGMGQRSSLFIRGTNSSHVLVLIDGVRLNQAGITGASDLSQIPISLVQRIEYIRGPRSAVYGSDAIGGVINI
LTGRDKPGTTLSAGLGSNGYQTYDGSTQQKLGEDTTVTLAGNYTYSKGYDVVAGMPGAGGPRQPDRDGFMGKMLWAGLEH
QFNEQFNGFARVYGFDNRSDYDGYTNYSNPLALIDTRKLSSRTYDTGLRYKNGIYASQFIASYNRTKDYNYSPLFGQHDI
TASLDEAEQYNLQWGNTFQLTNGMISAGADWQEQRTERKSSNQNTTADFTQHNTGIYLTGQQQISDVTLEGAVRSDDNSQ
FGWHSTWQTSAGWEFIDGYRLIGSYGTAYKAPNLMQLYSAYGGNANLKPEESKQWEGGVEGLTGPLTWRLSAYRNDIDQL
IDYSNLTNGYFNINKATIKGVEWTGSFDTGPLSHQVTLEYLDPRNADTHEILVRRAKQQVKYQLDWQVADLDWSVTYQYL
GQRYDKDYSTYPEETVELGGVSLWDLAVSYPVTSHLTVRGRIANLFDKDYEMVYGYQTPGREYYFTGSYNF

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=623, Percent_Identity=65.008025682183, Blast_Score=805, Evalue=0.0,
Organism=Escherichia coli, GI1788478, Length=679, Percent_Identity=26.3622974963181, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1786798, Length=774, Percent_Identity=24.6770025839793, Blast_Score=108, Evalue=7e-25,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010101
- InterPro:   IPR010917 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 70510; Mature: 70510

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS01156 TONB_DEPENDENT_REC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIKNTMTIKKYTLLTALSVTAFSGWAQGNNTTDNNDEMVVTANRFPQPKSSVLAPVDVV
CEECCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCHHHCCCCHHHH
TRADIDRWQSTNINDVLRRLPGVDIAQDGGMGQRSSLFIRGTNSSHVLVLIDGVRLNQAG
HHCCHHHHCCCCHHHHHHHCCCCCEECCCCCCCCCEEEEEECCCCEEEEEEECEEECCCC
ITGASDLSQIPISLVQRIEYIRGPRSAVYGSDAIGGVINILTGRDKPGTTLSAGLGSNGY
CCCCCHHHHHHHHHHHHHHHHCCCCHHCCCCCHHCCCEEEEECCCCCCCEEEECCCCCCC
QTYDGSTQQKLGEDTTVTLAGNYTYSKGYDVVAGMPGAGGPRQPDRDGFMGKMLWAGLEH
CCCCCCHHHHCCCCCEEEEEECEEECCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHH
QFNEQFNGFARVYGFDNRSDYDGYTNYSNPLALIDTRKLSSRTYDTGLRYKNGIYASQFI
HHHHHCCCEEEEEECCCCCCCCCCCCCCCCEEEEECHHHCCCCHHCCCCCCCCCHHHHHH
ASYNRTKDYNYSPLFGQHDITASLDEAEQYNLQWGNTFQLTNGMISAGADWQEQRTERKS
HHHCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCEEEECCCEEECCCCHHHHHHHHHC
SNQNTTADFTQHNTGIYLTGQQQISDVTLEGAVRSDDNSQFGWHSTWQTSAGWEFIDGYR
CCCCCCCEEEECCCCEEEECCHHCCCEEEECEEECCCCCCCCCCCCCCCCCCCEECCCEE
LIGSYGTAYKAPNLMQLYSAYGGNANLKPEESKQWEGGVEGLTGPLTWRLSAYRNDIDQL
EECCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHH
IDYSNLTNGYFNINKATIKGVEWTGSFDTGPLSHQVTLEYLDPRNADTHEILVRRAKQQV
HHHCCCCCCEEEEEEEEEEEEEEECCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHC
KYQLDWQVADLDWSVTYQYLGQRYDKDYSTYPEETVELGGVSLWDLAVSYPVTSHLTVRG
EEEEEEEEECCCHHHHHHHHHHHHCCCCCCCCHHHHHHCCEEEEHEEECCCCCCEEEEEE
RIANLFDKDYEMVYGYQTPGREYYFTGSYNF
HHHHHHCCCCEEEEEECCCCCEEEEECCCCC
>Mature Secondary Structure
MIIKNTMTIKKYTLLTALSVTAFSGWAQGNNTTDNNDEMVVTANRFPQPKSSVLAPVDVV
CEECCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCHHHCCCCHHHH
TRADIDRWQSTNINDVLRRLPGVDIAQDGGMGQRSSLFIRGTNSSHVLVLIDGVRLNQAG
HHCCHHHHCCCCHHHHHHHCCCCCEECCCCCCCCCEEEEEECCCCEEEEEEECEEECCCC
ITGASDLSQIPISLVQRIEYIRGPRSAVYGSDAIGGVINILTGRDKPGTTLSAGLGSNGY
CCCCCHHHHHHHHHHHHHHHHCCCCHHCCCCCHHCCCEEEEECCCCCCCEEEECCCCCCC
QTYDGSTQQKLGEDTTVTLAGNYTYSKGYDVVAGMPGAGGPRQPDRDGFMGKMLWAGLEH
CCCCCCHHHHCCCCCEEEEEECEEECCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHH
QFNEQFNGFARVYGFDNRSDYDGYTNYSNPLALIDTRKLSSRTYDTGLRYKNGIYASQFI
HHHHHCCCEEEEEECCCCCCCCCCCCCCCCEEEEECHHHCCCCHHCCCCCCCCCHHHHHH
ASYNRTKDYNYSPLFGQHDITASLDEAEQYNLQWGNTFQLTNGMISAGADWQEQRTERKS
HHHCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCEEEECCCEEECCCCHHHHHHHHHC
SNQNTTADFTQHNTGIYLTGQQQISDVTLEGAVRSDDNSQFGWHSTWQTSAGWEFIDGYR
CCCCCCCEEEECCCCEEEECCHHCCCEEEECEEECCCCCCCCCCCCCCCCCCCEECCCEE
LIGSYGTAYKAPNLMQLYSAYGGNANLKPEESKQWEGGVEGLTGPLTWRLSAYRNDIDQL
EECCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHH
IDYSNLTNGYFNINKATIKGVEWTGSFDTGPLSHQVTLEYLDPRNADTHEILVRRAKQQV
HHHCCCCCCEEEEEEEEEEEEEEECCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHC
KYQLDWQVADLDWSVTYQYLGQRYDKDYSTYPEETVELGGVSLWDLAVSYPVTSHLTVRG
EEEEEEEEECCCHHHHHHHHHHHHCCCCCCCCHHHHHHCCEEEEHEEECCCCCCEEEEEE
RIANLFDKDYEMVYGYQTPGREYYFTGSYNF
HHHHHHCCCCEEEEEECCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA