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The map label for this gene is acoC [H]

Identifier: 227827163

GI number: 227827163

Start: 795519

End: 796703

Strand: Direct

Name: acoC [H]

Synonym: M1425_0843

Alternate gene names: 227827163

Gene position: 795519-796703 (Clockwise)

Preceding gene: 227827162

Following gene: 227827170

Centisome position: 30.49

GC content: 35.95

Gene sequence:

>1185_bases
ATGGGCAAAGAAGTATTAATGCCAAAGTTAGGTTTAACAATGACTAAAGGGAAAATAGTTCAATGGAAGAAAAAAGAAGG
AGATAGAGTTCAAGAAGGAGAGGACTTAGTAATAATAGAAACTGAGAAAATAACAACTGCAGTAAAAGCAGCAGCTAGCG
GTATCTTACTTAAGATTTACGCCAAAGAAGGTGAAGAGGTACCTGTTGGTCAAATAATAGCGTACATAGGTGAAATTGGA
GAAAAACCCCCATCCTTATCTACTAAACCCACTTTGGTTAGTGAACAGCAACAAGGGCAACCAACTCGAATTGAAGAGGC
TAAGGCAATAAGTGAAGTAAGAGCTTCACCTAGAGCTAGAAGGCTTGCTAAAGAAAAAGGAATAGATCTAGTTAAAATAA
AGGGGAGTGGACCTGGAGGTATGATAACAGAAGATGATGTAATTAGAGAGTTAGAAAATATTGAAAAAGGAGTGAAATTC
ACTGCCACTGGTCTTAGAGTTAAAGAAGTGATACCAATGACTGCAATAAGGCAAGAAATAAGTAGAAGAATGGTTCAGAG
CCTTCAGACAATGGCCCAAGTGACACTAAATATTGAGATTAATGCAAATTCGTTAGTTAAAATAAAAAATGAAGTAGAGT
CTAAGTATAGCATGAAAATTACTTATACAGACGTTCTAGTTAAAGTAGTAGCTAAACTCATCAGAAGTCATCCATTTCTA
AACGCAACACTAGAGGGCGATCAAATAAAGATAATTGAGGATGTTAATATAGGAATAGCTGTGGCTCTAGATCAGGGATT
AATAGTACCAGTAATAAGGAACGCAGATATTAAGCCAATTACTGAAATAGCAAAAGAAAGTCATGAACTAGCGGATAAGG
CTAGAGAAAACAAGTTAAATCCCGACGAAGTAACAGGAGGAACGTTTACGATAAGTAATTTAGGAATGTATGATATAGAT
TCATTTACACCAATTATAAATCCTCCTCAAACTGCTATATTGGGAGTAGGTAGGATAAGAAAGGCACCAGTTGTAATAGG
CGATAATATCTCAATCGGTTACGTTATGTGGCTAAGTTTAACATTTGATCATAGAGTGTTAGATGGACATACCGCTGCTA
AGTTCTTGAAAGAGCTTACTGAAATATTAGAGGATGAGAATAGATTGAGAGCGTTTTTAAGTTAG

Upstream 100 bases:

>100_bases
TAATGATCAGAATAGATAGAGATGGTCCTCTCTTAGTTAGCATATCCAAGTCTTTAAATTATATAAACACTTATTATAAG
GAAGAGATGGGTGAATTAAG

Downstream 100 bases:

>100_bases
GAATCTATTCCATCTTGAACGGTAAATCGAAAAACACCTTATTTAGGTAATTAGTAAGTGTTTAATTATTTGCGCTTGGG
ATGCTCTCTTTTGGGATGCT

Product: catalytic domain of components of variousdehydrogenase complexes

Products: NA

Alternate protein names: Acetoin dehydrogenase E2 component; Dihydrolipoamide acetyltransferase component of acetoin cleaving system [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MGKEVLMPKLGLTMTKGKIVQWKKKEGDRVQEGEDLVIIETEKITTAVKAAASGILLKIYAKEGEEVPVGQIIAYIGEIG
EKPPSLSTKPTLVSEQQQGQPTRIEEAKAISEVRASPRARRLAKEKGIDLVKIKGSGPGGMITEDDVIRELENIEKGVKF
TATGLRVKEVIPMTAIRQEISRRMVQSLQTMAQVTLNIEINANSLVKIKNEVESKYSMKITYTDVLVKVVAKLIRSHPFL
NATLEGDQIKIIEDVNIGIAVALDQGLIVPVIRNADIKPITEIAKESHELADKARENKLNPDEVTGGTFTISNLGMYDID
SFTPIINPPQTAILGVGRIRKAPVVIGDNISIGYVMWLSLTFDHRVLDGHTAAKFLKELTEILEDENRLRAFLS

Sequences:

>Translated_394_residues
MGKEVLMPKLGLTMTKGKIVQWKKKEGDRVQEGEDLVIIETEKITTAVKAAASGILLKIYAKEGEEVPVGQIIAYIGEIG
EKPPSLSTKPTLVSEQQQGQPTRIEEAKAISEVRASPRARRLAKEKGIDLVKIKGSGPGGMITEDDVIRELENIEKGVKF
TATGLRVKEVIPMTAIRQEISRRMVQSLQTMAQVTLNIEINANSLVKIKNEVESKYSMKITYTDVLVKVVAKLIRSHPFL
NATLEGDQIKIIEDVNIGIAVALDQGLIVPVIRNADIKPITEIAKESHELADKARENKLNPDEVTGGTFTISNLGMYDID
SFTPIINPPQTAILGVGRIRKAPVVIGDNISIGYVMWLSLTFDHRVLDGHTAAKFLKELTEILEDENRLRAFLS
>Mature_393_residues
GKEVLMPKLGLTMTKGKIVQWKKKEGDRVQEGEDLVIIETEKITTAVKAAASGILLKIYAKEGEEVPVGQIIAYIGEIGE
KPPSLSTKPTLVSEQQQGQPTRIEEAKAISEVRASPRARRLAKEKGIDLVKIKGSGPGGMITEDDVIRELENIEKGVKFT
ATGLRVKEVIPMTAIRQEISRRMVQSLQTMAQVTLNIEINANSLVKIKNEVESKYSMKITYTDVLVKVVAKLIRSHPFLN
ATLEGDQIKIIEDVNIGIAVALDQGLIVPVIRNADIKPITEIAKESHELADKARENKLNPDEVTGGTFTISNLGMYDIDS
FTPIINPPQTAILGVGRIRKAPVVIGDNISIGYVMWLSLTFDHRVLDGHTAAKFLKELTEILEDENRLRAFLS

Specific function: The 2-Oxoglutarate Dehydrogenase Complex Catalyzes The Overall Conversion Of 2-Oxoglutarate To Succinyl-CoA And Co(2). It Contains Multiple Copies Of Three Enzymatic Components: 2- Oxoglutarate Dehydrogenase (E1), Dihydrolipoamide Succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=424, Percent_Identity=33.4905660377358, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI203098753, Length=444, Percent_Identity=30.4054054054054, Blast_Score=193, Evalue=3e-49,
Organism=Homo sapiens, GI203098816, Length=444, Percent_Identity=30.4054054054054, Blast_Score=192, Evalue=3e-49,
Organism=Homo sapiens, GI19923748, Length=228, Percent_Identity=35.5263157894737, Blast_Score=148, Evalue=8e-36,
Organism=Homo sapiens, GI260898739, Length=166, Percent_Identity=39.7590361445783, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI110671329, Length=408, Percent_Identity=25, Blast_Score=119, Evalue=5e-27,
Organism=Escherichia coli, GI1786946, Length=401, Percent_Identity=31.6708229426434, Blast_Score=210, Evalue=1e-55,
Organism=Escherichia coli, GI1786305, Length=424, Percent_Identity=28.3018867924528, Blast_Score=139, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17560088, Length=429, Percent_Identity=31.9347319347319, Blast_Score=187, Evalue=8e-48,
Organism=Caenorhabditis elegans, GI25146366, Length=401, Percent_Identity=28.6783042394015, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17537937, Length=413, Percent_Identity=26.1501210653753, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17538894, Length=228, Percent_Identity=33.7719298245614, Blast_Score=133, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6320352, Length=391, Percent_Identity=32.4808184143223, Blast_Score=184, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6324258, Length=440, Percent_Identity=31.1363636363636, Blast_Score=180, Evalue=3e-46,
Organism=Drosophila melanogaster, GI24582497, Length=288, Percent_Identity=33.6805555555556, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI20129315, Length=288, Percent_Identity=33.6805555555556, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24645909, Length=226, Percent_Identity=33.6283185840708, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI18859875, Length=400, Percent_Identity=26, Blast_Score=135, Evalue=6e-32,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 43403; Mature: 43272

Theoretical pI: Translated: 7.80; Mature: 7.80

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKEVLMPKLGLTMTKGKIVQWKKKEGDRVQEGEDLVIIETEKITTAVKAAASGILLKIY
CCCCCCCCCCCCEECCCCEEEEECCCCCCCCCCCCEEEEECHHHHHHHHHHHCCEEEEEE
AKEGEEVPVGQIIAYIGEIGEKPPSLSTKPTLVSEQQQGQPTRIEEAKAISEVRASPRAR
ECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHCCHHHH
RLAKEKGIDLVKIKGSGPGGMITEDDVIRELENIEKGVKFTATGLRVKEVIPMTAIRQEI
HHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCEEEECCEEHHHHHHHHHHHHHH
SRRMVQSLQTMAQVTLNIEINANSLVKIKNEVESKYSMKITYTDVLVKVVAKLIRSHPFL
HHHHHHHHHHHHEEEEEEEECCCCEEEEHHHHCCEEEEEEEHHHHHHHHHHHHHHCCCCE
NATLEGDQIKIIEDVNIGIAVALDQGLIVPVIRNADIKPITEIAKESHELADKARENKLN
EEEECCCEEEEEEECCCEEEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCC
PDEVTGGTFTISNLGMYDIDSFTPIINPPQTAILGVGRIRKAPVVIGDNISIGYVMWLSL
CCCCCCCEEEECCCCEEECCCCCCCCCCCHHHHEECCCCCCCCEEECCCCCEEEEEEEEE
TFDHRVLDGHTAAKFLKELTEILEDENRLRAFLS
EECCEECCCHHHHHHHHHHHHHHCCCHHHHHHCC
>Mature Secondary Structure 
GKEVLMPKLGLTMTKGKIVQWKKKEGDRVQEGEDLVIIETEKITTAVKAAASGILLKIY
CCCCCCCCCCCEECCCCEEEEECCCCCCCCCCCCEEEEECHHHHHHHHHHHCCEEEEEE
AKEGEEVPVGQIIAYIGEIGEKPPSLSTKPTLVSEQQQGQPTRIEEAKAISEVRASPRAR
ECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHCCHHHH
RLAKEKGIDLVKIKGSGPGGMITEDDVIRELENIEKGVKFTATGLRVKEVIPMTAIRQEI
HHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCEEEECCEEHHHHHHHHHHHHHH
SRRMVQSLQTMAQVTLNIEINANSLVKIKNEVESKYSMKITYTDVLVKVVAKLIRSHPFL
HHHHHHHHHHHHEEEEEEEECCCCEEEEHHHHCCEEEEEEEHHHHHHHHHHHHHHCCCCE
NATLEGDQIKIIEDVNIGIAVALDQGLIVPVIRNADIKPITEIAKESHELADKARENKLN
EEEECCCEEEEEEECCCEEEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCC
PDEVTGGTFTISNLGMYDIDSFTPIINPPQTAILGVGRIRKAPVVIGDNISIGYVMWLSL
CCCCCCCEEEECCCCEEECCCCCCCCCCCHHHHEECCCCCCCCEEECCCCCEEEEEEEEE
TFDHRVLDGHTAAKFLKELTEILEDENRLRAFLS
EECCEECCCHHHHHHHHHHHHHHCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]