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The map label for this gene is narI [H]

Identifier: 227827155

GI number: 227827155

Start: 786180

End: 786881

Strand: Direct

Name: narI [H]

Synonym: M1425_0831

Alternate gene names: 227827155

Gene position: 786180-786881 (Clockwise)

Preceding gene: 227827154

Following gene: 227827156

Centisome position: 30.14

GC content: 33.19

Gene sequence:

>702_bases
ATGTCTGACCTCTTAAATCTGCTGTTATGGGTTGTGTATCCATATATATCGATTACAATTTTTCTTACTGGTCATGTATA
CACATATACATTAAGAAGATATTATTGGAGCGCCAGATCTACTGAGTTATTAGAAAAAAGGATGCAATCGTGGTCCTCAT
ATCTTTTTCATTATGGAATAATTATTGTATTAATAGGGCATATAGTAGGAATAGCAATTCCGACAGCAATCTTACTCGCA
CTAGGTATATCGCTAACTTTGCACACGGAACTTGCATTTTATTTAGGAGGAATATTTGGAGTTATTACACTAATAGGAAT
TATAGGATTAATCACAAGATATCTTACTAATCCTAGAGTAAGAGTTACATTAAGATTTACTGATTATCTCGTGTATATAA
TTCTGATAGTAACTATAGTCTTAGGTCTATATAATACATTAGTAGTGCACCCAGAGTACGAAACTACTGTAGGACCGTGG
TTACAAGGTTTACTATCGCTTCATCCTAACCCGAATTTCATGAATGACGTTCCTTTATTACTAAAAGTTCATATAGCAGT
CTCAATGTTCCTTTATATAATATGGCCTTTTAGTAGATTAGTTCATGTATGGAGCTTTCCAGTAACCTATTTATGGAGAC
CTTATATAATATATAGAAGCCCACTAGTGAAGATTAGACAAGTCAAAGACAAACAATGGTGA

Upstream 100 bases:

>100_bases
CTTCCATTGAAGGAGTTAACCATCAACCTAAAAGCTAAGAATTCAATTTTTTATTATATTCTCGTTTCATTATATAAGGA
ATTGTATTTAGGTGATCCAG

Downstream 100 bases:

>100_bases
CTATTAATGTCGGAGTTGACTGGTTTTACAGATGCTATTAAACGACTTCATCCAGCATATCTTGCAGTAGTAATGGGTAT
CGGTATTATTTCAATAGATG

Product: respiratory nitrate reductase, subunit gamma

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 233; Mature: 232

Protein sequence:

>233_residues
MSDLLNLLLWVVYPYISITIFLTGHVYTYTLRRYYWSARSTELLEKRMQSWSSYLFHYGIIIVLIGHIVGIAIPTAILLA
LGISLTLHTELAFYLGGIFGVITLIGIIGLITRYLTNPRVRVTLRFTDYLVYIILIVTIVLGLYNTLVVHPEYETTVGPW
LQGLLSLHPNPNFMNDVPLLLKVHIAVSMFLYIIWPFSRLVHVWSFPVTYLWRPYIIYRSPLVKIRQVKDKQW

Sequences:

>Translated_233_residues
MSDLLNLLLWVVYPYISITIFLTGHVYTYTLRRYYWSARSTELLEKRMQSWSSYLFHYGIIIVLIGHIVGIAIPTAILLA
LGISLTLHTELAFYLGGIFGVITLIGIIGLITRYLTNPRVRVTLRFTDYLVYIILIVTIVLGLYNTLVVHPEYETTVGPW
LQGLLSLHPNPNFMNDVPLLLKVHIAVSMFLYIIWPFSRLVHVWSFPVTYLWRPYIIYRSPLVKIRQVKDKQW
>Mature_232_residues
SDLLNLLLWVVYPYISITIFLTGHVYTYTLRRYYWSARSTELLEKRMQSWSSYLFHYGIIIVLIGHIVGIAIPTAILLAL
GISLTLHTELAFYLGGIFGVITLIGIIGLITRYLTNPRVRVTLRFTDYLVYIILIVTIVLGLYNTLVVHPEYETTVGPWL
QGLLSLHPNPNFMNDVPLLLKVHIAVSMFLYIIWPFSRLVHVWSFPVTYLWRPYIIYRSPLVKIRQVKDKQW

Specific function: The Nitrate Reductase Enzyme Complex Allows E.Coli To Use Nitrate As An Electron Acceptor During Anaerobic Growth. The Gamma Chain Is A Membrane-Embedded Heme-Iron Unit Resembling Cytochrome B, Which Transfers Electrons From Quinones To The Beta Subunit.

COG id: COG2181

COG function: function code C; Nitrate reductase gamma subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787480, Length=223, Percent_Identity=31.390134529148, Blast_Score=129, Evalue=1e-31,
Organism=Escherichia coli, GI1787738, Length=228, Percent_Identity=37.280701754386, Blast_Score=128, Evalue=4e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003816 [H]

Pfam domain/function: PF02665 Nitrate_red_gam [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 27145; Mature: 27014

Theoretical pI: Translated: 9.85; Mature: 9.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLLNLLLWVVYPYISITIFLTGHVYTYTLRRYYWSARSTELLEKRMQSWSSYLFHYGI
CHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
IIVLIGHIVGIAIPTAILLALGISLTLHTELAFYLGGIFGVITLIGIIGLITRYLTNPRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
RVTLRFTDYLVYIILIVTIVLGLYNTLVVHPEYETTVGPWLQGLLSLHPNPNFMNDVPLL
EEEEEHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHCCCCCCCCCCHHHH
LKVHIAVSMFLYIIWPFSRLVHVWSFPVTYLWRPYIIYRSPLVKIRQVKDKQW
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHCCCCC
>Mature Secondary Structure 
SDLLNLLLWVVYPYISITIFLTGHVYTYTLRRYYWSARSTELLEKRMQSWSSYLFHYGI
HHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
IIVLIGHIVGIAIPTAILLALGISLTLHTELAFYLGGIFGVITLIGIIGLITRYLTNPRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
RVTLRFTDYLVYIILIVTIVLGLYNTLVVHPEYETTVGPWLQGLLSLHPNPNFMNDVPLL
EEEEEHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHCCCCCCCCCCHHHH
LKVHIAVSMFLYIIWPFSRLVHVWSFPVTYLWRPYIIYRSPLVKIRQVKDKQW
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8846791; 9353933; 9384377; 7557333 [H]