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The map label for this gene is narH [H]

Identifier: 227827153

GI number: 227827153

Start: 784203

End: 785684

Strand: Direct

Name: narH [H]

Synonym: M1425_0829

Alternate gene names: 227827153

Gene position: 784203-785684 (Clockwise)

Preceding gene: 227827152

Following gene: 227827154

Centisome position: 30.06

GC content: 38.73

Gene sequence:

>1482_bases
ATGAAAGTTATGGTACAATTTGCGGCGGTATTTAATTTAGATAAATGCTTGGGATGTCACGCTTGCAGCATTGCATGCAA
GAATCTATGGACTAATAGAGAAGGTACTGAGTATATGTGGTGGAATAACGTTGAAACTAAGCCAGGTCCAGGATATCCGA
ATCAGTGGGAGGATCAAGATAAATGGCATGGCGGATGGATATTAACTAAAGATGGAAAACTAAAGTTAGCGATAGGAGGA
AGGATAGCTAGGCTACTGGAACTCTTCTCTAATCCTTATTTACCTACAATTGACGACTATTACGAGCCGTTTACTTATAC
ATATAGCGATTTAGTAAAACAAGATGATGGAAAACAGCCAGTAGCGAAGCCAATCTCGTTAATCTCTAAAAAACCGATTG
AAATAGACAAGAGTCCTAACTGGAATGATGATCTGGCTGGAGGAACGGAGCTAATATTACAAGATCCTAATGTCAAGAAA
CTGCAAGATAAAATAAAGACCGATTTCGAGAATGCCTTCATGATGTATTTGCCGAGAATATGCAACCATTGTCTTAACCC
CTCATGTATGGCGGCTTGTCCTGCAGGTGCTATTTATAAGAGAGTAGAAGATGGGATAGTGCTCACAGATCAACAGAAAT
GTAGAGGTTGGAGATTCTGTATAGCAGCTTGTCCATATAAAAAGGTATATTATAATTGGAGTACTGGAAAAGCCGAAAAA
TGCATACTGTGTTATCCAAGGATAGAGAATGGACAACCGCCAGCTTGTTTCCAAGAATGCGTCGGTAGGATTAGATACAT
TGGTCCCGTATTCTATGACGCTGACAGAGTTTTGTGGGCTGCCTCTGCTGGAGACCCTAAAGAAATTATTGATAGATATC
TTGAGATTATCTTAAATCCTTATGATCCAGATGTCGTAAAAAATGCTAAGGAAAATGGAATCGATGAGGACTTCATAAAA
GCAGCTCAAAGAACGCCTGTTTATAAAATGATGAAAGCGTGGGGAATTGCGTTACCGTTACATCCCGAATATAGAACACT
GCCTATGGTATGGTATATCCCACCTTTAAGCCCAATCGTGGAAAACATGAAGACTTCGGATGAACAAATATTCCCGTTAG
TAGATCAAATGAGAATTCCACTAGAATACTTAGCGTCGCTATTTACTGCTGGAGATGCTGATAGGGTTAAGAATGTTTTG
AAAAAATTGTTGGCTCTAAGAATTTACCAAAGATATATAAGATTACACAAAAATCCCCCAGGATGGATATTCAAAGAAAC
TAGCCTAACTGAAAAAGATTTTGAAGAAATGTATAAAGTGCTGGCCATAGCAAAAATGGAGGATAGATTTGTAATACCAA
CGGCACATAAAGAAAAAGCAATCAAGATGTTTGAAGAGGAAATAACTCCAGAATATGGGCAAGGGCATAGAGGTATGGAA
ATTGCTGCTAAAAAGCCTAGAGATTTGAGGTTGAGAGCATGA

Upstream 100 bases:

>100_bases
GGTTAATCGGTGGATATGCACAACTCTCCTATGCTCTTAATTATTATGGAGCAGTTGGTACTCAAAGAGATACAATAGTA
GCTGTAAGGAGGTGGAGAAC

Downstream 100 bases:

>100_bases
ATAGGGAGCTTCTTGTTATCATAGCGGATTTGCTTGAATATCCTGCTTATTGGCTTCCCAAATTAAGTGAATTTGAGGCA
AAATTAGTCAAGATAAATGA

Product: nitrate reductase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MKVMVQFAAVFNLDKCLGCHACSIACKNLWTNREGTEYMWWNNVETKPGPGYPNQWEDQDKWHGGWILTKDGKLKLAIGG
RIARLLELFSNPYLPTIDDYYEPFTYTYSDLVKQDDGKQPVAKPISLISKKPIEIDKSPNWNDDLAGGTELILQDPNVKK
LQDKIKTDFENAFMMYLPRICNHCLNPSCMAACPAGAIYKRVEDGIVLTDQQKCRGWRFCIAACPYKKVYYNWSTGKAEK
CILCYPRIENGQPPACFQECVGRIRYIGPVFYDADRVLWAASAGDPKEIIDRYLEIILNPYDPDVVKNAKENGIDEDFIK
AAQRTPVYKMMKAWGIALPLHPEYRTLPMVWYIPPLSPIVENMKTSDEQIFPLVDQMRIPLEYLASLFTAGDADRVKNVL
KKLLALRIYQRYIRLHKNPPGWIFKETSLTEKDFEEMYKVLAIAKMEDRFVIPTAHKEKAIKMFEEEITPEYGQGHRGME
IAAKKPRDLRLRA

Sequences:

>Translated_493_residues
MKVMVQFAAVFNLDKCLGCHACSIACKNLWTNREGTEYMWWNNVETKPGPGYPNQWEDQDKWHGGWILTKDGKLKLAIGG
RIARLLELFSNPYLPTIDDYYEPFTYTYSDLVKQDDGKQPVAKPISLISKKPIEIDKSPNWNDDLAGGTELILQDPNVKK
LQDKIKTDFENAFMMYLPRICNHCLNPSCMAACPAGAIYKRVEDGIVLTDQQKCRGWRFCIAACPYKKVYYNWSTGKAEK
CILCYPRIENGQPPACFQECVGRIRYIGPVFYDADRVLWAASAGDPKEIIDRYLEIILNPYDPDVVKNAKENGIDEDFIK
AAQRTPVYKMMKAWGIALPLHPEYRTLPMVWYIPPLSPIVENMKTSDEQIFPLVDQMRIPLEYLASLFTAGDADRVKNVL
KKLLALRIYQRYIRLHKNPPGWIFKETSLTEKDFEEMYKVLAIAKMEDRFVIPTAHKEKAIKMFEEEITPEYGQGHRGME
IAAKKPRDLRLRA
>Mature_493_residues
MKVMVQFAAVFNLDKCLGCHACSIACKNLWTNREGTEYMWWNNVETKPGPGYPNQWEDQDKWHGGWILTKDGKLKLAIGG
RIARLLELFSNPYLPTIDDYYEPFTYTYSDLVKQDDGKQPVAKPISLISKKPIEIDKSPNWNDDLAGGTELILQDPNVKK
LQDKIKTDFENAFMMYLPRICNHCLNPSCMAACPAGAIYKRVEDGIVLTDQQKCRGWRFCIAACPYKKVYYNWSTGKAEK
CILCYPRIENGQPPACFQECVGRIRYIGPVFYDADRVLWAASAGDPKEIIDRYLEIILNPYDPDVVKNAKENGIDEDFIK
AAQRTPVYKMMKAWGIALPLHPEYRTLPMVWYIPPLSPIVENMKTSDEQIFPLVDQMRIPLEYLASLFTAGDADRVKNVL
KKLLALRIYQRYIRLHKNPPGWIFKETSLTEKDFEEMYKVLAIAKMEDRFVIPTAHKEKAIKMFEEEITPEYGQGHRGME
IAAKKPRDLRLRA

Specific function: The Nitrate Reductase Enzyme Complex Allows E. coli To Use Nitrate As An Electron Acceptor During Anaerobic Growth. The Beta Chain Is An Electron Transfer Unit Containing Four Cysteine Clusters Involved In The Formation Of Iron-Sulfur Centers. Electrons A

COG id: COG1140

COG function: function code C; Nitrate reductase beta subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787478, Length=473, Percent_Identity=53.276955602537, Blast_Score=543, Evalue=1e-155,
Organism=Escherichia coli, GI1787740, Length=472, Percent_Identity=52.3305084745763, Blast_Score=535, Evalue=1e-153,
Organism=Escherichia coli, GI1787122, Length=101, Percent_Identity=43.5643564356436, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787872, Length=101, Percent_Identity=43.5643564356436, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1789370, Length=125, Percent_Identity=35.2, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1790326, Length=163, Percent_Identity=31.9018404907975, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1787749, Length=87, Percent_Identity=36.7816091954023, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI226510944, Length=96, Percent_Identity=39.5833333333333, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI2367345, Length=101, Percent_Identity=30.6930693069307, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006547 [H]

Pfam domain/function: NA

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 56823; Mature: 56823

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVMVQFAAVFNLDKCLGCHACSIACKNLWTNREGTEYMWWNNVETKPGPGYPNQWEDQD
CCCCEEHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
KWHGGWILTKDGKLKLAIGGRIARLLELFSNPYLPTIDDYYEPFTYTYSDLVKQDDGKQP
CCCCCEEEECCCEEEEEECHHHHHHHHHHCCCCCCCHHHHHCCCCCCHHHHHHCCCCCCH
VAKPISLISKKPIEIDKSPNWNDDLAGGTELILQDPNVKKLQDKIKTDFENAFMMYLPRI
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
CNHCLNPSCMAACPAGAIYKRVEDGIVLTDQQKCRGWRFCIAACPYKKVYYNWSTGKAEK
HHHHCCCHHHHHCCCHHHHHHHHCCEEEECHHHCCCEEEEEEECCCEEEEEECCCCCCCE
CILCYPRIENGQPPACFQECVGRIRYIGPVFYDADRVLWAASAGDPKEIIDRYLEIILNP
EEEEECCCCCCCCCHHHHHHHHHHHHHCHHEECCCCEEEEECCCCHHHHHHHHHHHHCCC
YDPDVVKNAKENGIDEDFIKAAQRTPVYKMMKAWGIALPLHPEYRTLPMVWYIPPLSPIV
CCCHHHHCCHHCCCCHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCEEEEECCHHHHH
ENMKTSDEQIFPLVDQMRIPLEYLASLFTAGDADRVKNVLKKLLALRIYQRYIRLHKNPP
HHHCCCCHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GWIFKETSLTEKDFEEMYKVLAIAKMEDRFVIPTAHKEKAIKMFEEEITPEYGQGHRGME
CCEEECCCCCHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHCCCCCCCCCCCCE
IAAKKPRDLRLRA
EECCCCCCCCCCC
>Mature Secondary Structure
MKVMVQFAAVFNLDKCLGCHACSIACKNLWTNREGTEYMWWNNVETKPGPGYPNQWEDQD
CCCCEEHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
KWHGGWILTKDGKLKLAIGGRIARLLELFSNPYLPTIDDYYEPFTYTYSDLVKQDDGKQP
CCCCCEEEECCCEEEEEECHHHHHHHHHHCCCCCCCHHHHHCCCCCCHHHHHHCCCCCCH
VAKPISLISKKPIEIDKSPNWNDDLAGGTELILQDPNVKKLQDKIKTDFENAFMMYLPRI
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
CNHCLNPSCMAACPAGAIYKRVEDGIVLTDQQKCRGWRFCIAACPYKKVYYNWSTGKAEK
HHHHCCCHHHHHCCCHHHHHHHHCCEEEECHHHCCCEEEEEEECCCEEEEEECCCCCCCE
CILCYPRIENGQPPACFQECVGRIRYIGPVFYDADRVLWAASAGDPKEIIDRYLEIILNP
EEEEECCCCCCCCCHHHHHHHHHHHHHCHHEECCCCEEEEECCCCHHHHHHHHHHHHCCC
YDPDVVKNAKENGIDEDFIKAAQRTPVYKMMKAWGIALPLHPEYRTLPMVWYIPPLSPIV
CCCHHHHCCHHCCCCHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCEEEEECCHHHHH
ENMKTSDEQIFPLVDQMRIPLEYLASLFTAGDADRVKNVLKKLLALRIYQRYIRLHKNPP
HHHCCCCHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GWIFKETSLTEKDFEEMYKVLAIAKMEDRFVIPTAHKEKAIKMFEEEITPEYGQGHRGME
CCEEECCCCCHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHCCCCCCCCCCCCE
IAAKKPRDLRLRA
EECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8846791; 9353933; 7557333; 9384377 [H]