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The map label for this gene is yitF [H]

Identifier: 227827138

GI number: 227827138

Start: 761773

End: 762885

Strand: Direct

Name: yitF [H]

Synonym: M1425_0812

Alternate gene names: 227827138

Gene position: 761773-762885 (Clockwise)

Preceding gene: 227827136

Following gene: 227827143

Centisome position: 29.2

GC content: 34.5

Gene sequence:

>1113_bases
ATGAAAATAAGCGATATTAAAACGTATAGAGTTAAAATACCATTAAGAGAGTCTATACAATTCTCATGGGAACCATTTCC
TAATACCCACTTTGAATTTATATTAATAGAAGTTGTAAATAGTAAGGGTATTAAAGGGTATTCTGCGACACAGTTTACAG
CACAGACTGAGGTTGCGATAAGGGGTTTAAAACCATTATTAAAGGGATTAGATGTGGAGGACTTCTTAGCTAATGATAGG
CTTCTGGAATTGGGTAGTTGGTTTTTCGGAAGGATAGGCGCTATTGAAGTAGCCCTATTGGATCTCTTAGCTAAGGAAGA
GAACTTACCATTATATAAACTACTTAGGGGAAATAGAAAGAGAATTAGGGTATATGCTAGTACTGGAAGATTAGCCAAAG
CCGAGGAAATCATTAATTTAATTAAAAAATATTATGATATAGGTATTGACATAGTTAAAATAAGGTTTCACAGAATTACT
ATAAACGATGATTTAAACATAGTTAAAGAAATAAGAAAGGAGTTTGGAGAACAAATCAAAATTGCAGTAGATGCTAATCA
AGCTTGGGGATTAGTTTCCCCGTTCTGGAACAGATATGATGCGTTAAAAGTAGCTAAGGAGCTAGAGAGATACAATATAG
AATGGTTAGAGGAACCTCTTTTTAAGGACGACATAGAAGGTTATGCGTGGTTAAGAAGAAATACTAGTGTTAAGATAGCC
GCTGGAGAACTGGAGCATAACTTCTGGATATTTAAAAAGTTTATTGAAGAGAATGCTTTAGATATAGTTCAAGCTGATGC
AATCTACTCTAATGGAATTAGCGAGTGTATTAAGATAGCCTTAATGGCGCAAGTTAATGGTTTATTATTCTTACCCCACG
CATGGGATCCAGGATTAGGATGGTTAGCTAATTTGCATTTAACTGCAAGCTTACCAGAAAGTTTAACTCCTTATTTAGAA
ACTCCTTTAGATCCATTATGGTGGTTTAATGATGTAATTCAGTTTGCCTTTAAGGAAGGAATAGAAATACAGAACGGTTA
TGTGAGAGTACCAGAAAAAGAGGGGCTTGGGATTGAGTTGGATATGGAAAAGATTAAAAAATTCATTACATAG

Upstream 100 bases:

>100_bases
AATGATAAATTAAAATAAAACGTTTACTCATTTATAGTTAACTGATATATTAAAAGATTTGTCGAAAAGCTTAATTTTTA
CGAATGTTAAGAACTTAGTG

Downstream 100 bases:

>100_bases
CTATTTTTGACTTAAAATATTTGAATATTTGTCTCTTAAAGCCTTCTTATCTGTTTTTCCCGTACTAGTCTTAGGAAGCT
CTACTTCTAAAATTATAATC

Product: mandelate racemase/muconate lactonizing protein

Products: 3-chloro-cis,cis-muconate

Alternate protein names: NA

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MKISDIKTYRVKIPLRESIQFSWEPFPNTHFEFILIEVVNSKGIKGYSATQFTAQTEVAIRGLKPLLKGLDVEDFLANDR
LLELGSWFFGRIGAIEVALLDLLAKEENLPLYKLLRGNRKRIRVYASTGRLAKAEEIINLIKKYYDIGIDIVKIRFHRIT
INDDLNIVKEIRKEFGEQIKIAVDANQAWGLVSPFWNRYDALKVAKELERYNIEWLEEPLFKDDIEGYAWLRRNTSVKIA
AGELEHNFWIFKKFIEENALDIVQADAIYSNGISECIKIALMAQVNGLLFLPHAWDPGLGWLANLHLTASLPESLTPYLE
TPLDPLWWFNDVIQFAFKEGIEIQNGYVRVPEKEGLGIELDMEKIKKFIT

Sequences:

>Translated_370_residues
MKISDIKTYRVKIPLRESIQFSWEPFPNTHFEFILIEVVNSKGIKGYSATQFTAQTEVAIRGLKPLLKGLDVEDFLANDR
LLELGSWFFGRIGAIEVALLDLLAKEENLPLYKLLRGNRKRIRVYASTGRLAKAEEIINLIKKYYDIGIDIVKIRFHRIT
INDDLNIVKEIRKEFGEQIKIAVDANQAWGLVSPFWNRYDALKVAKELERYNIEWLEEPLFKDDIEGYAWLRRNTSVKIA
AGELEHNFWIFKKFIEENALDIVQADAIYSNGISECIKIALMAQVNGLLFLPHAWDPGLGWLANLHLTASLPESLTPYLE
TPLDPLWWFNDVIQFAFKEGIEIQNGYVRVPEKEGLGIELDMEKIKKFIT
>Mature_370_residues
MKISDIKTYRVKIPLRESIQFSWEPFPNTHFEFILIEVVNSKGIKGYSATQFTAQTEVAIRGLKPLLKGLDVEDFLANDR
LLELGSWFFGRIGAIEVALLDLLAKEENLPLYKLLRGNRKRIRVYASTGRLAKAEEIINLIKKYYDIGIDIVKIRFHRIT
INDDLNIVKEIRKEFGEQIKIAVDANQAWGLVSPFWNRYDALKVAKELERYNIEWLEEPLFKDDIEGYAWLRRNTSVKIA
AGELEHNFWIFKKFIEENALDIVQADAIYSNGISECIKIALMAQVNGLLFLPHAWDPGLGWLANLHLTASLPESLTPYLE
TPLDPLWWFNDVIQFAFKEGIEIQNGYVRVPEKEGLGIELDMEKIKKFIT

Specific function: D-galactonate degradation; first step. D-galactonate degradation; third step. [C]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI42544119, Length=412, Percent_Identity=23.3009708737864, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI186972148, Length=217, Percent_Identity=27.1889400921659, Blast_Score=79, Evalue=9e-15,
Organism=Homo sapiens, GI42544117, Length=410, Percent_Identity=22.9268292682927, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI48994953, Length=392, Percent_Identity=26.530612244898, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI226510960, Length=281, Percent_Identity=26.6903914590747, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1787864, Length=379, Percent_Identity=22.6912928759894, Blast_Score=81, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015654
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: 5.5.1.7

Molecular weight: Translated: 42746; Mature: 42746

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISDIKTYRVKIPLRESIQFSWEPFPNTHFEFILIEVVNSKGIKGYSATQFTAQTEVAI
CCCCCCEEEEEEECHHHCCEEECCCCCCCCEEEEEEEEECCCCCCCCCCCEEEHHHHHHH
RGLKPLLKGLDVEDFLANDRLLELGSWFFGRIGAIEVALLDLLAKEENLPLYKLLRGNRK
HHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCE
RIRVYASTGRLAKAEEIINLIKKYYDIGIDIVKIRFHRITINDDLNIVKEIRKEFGEQIK
EEEEEECCCCCHHHHHHHHHHHHHHHCCEEEEEEEEEEEEECCCHHHHHHHHHHCCCEEE
IAVDANQAWGLVSPFWNRYDALKVAKELERYNIEWLEEPLFKDDIEGYAWLRRNTSVKIA
EEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHCCCCEEEEECCCEEEEE
AGELEHNFWIFKKFIEENALDIVQADAIYSNGISECIKIALMAQVNGLLFLPHAWDPGLG
ECCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEECCCCCCCCC
WLANLHLTASLPESLTPYLETPLDPLWWFNDVIQFAFKEGIEIQNGYVRVPEKEGLGIEL
CEEEEEEEECCCCCCCHHHHCCCCHHHHHHHHHHHHHHCCCEECCCEEECCCCCCCEEEE
DMEKIKKFIT
CHHHHHHHCC
>Mature Secondary Structure
MKISDIKTYRVKIPLRESIQFSWEPFPNTHFEFILIEVVNSKGIKGYSATQFTAQTEVAI
CCCCCCEEEEEEECHHHCCEEECCCCCCCCEEEEEEEEECCCCCCCCCCCEEEHHHHHHH
RGLKPLLKGLDVEDFLANDRLLELGSWFFGRIGAIEVALLDLLAKEENLPLYKLLRGNRK
HHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCE
RIRVYASTGRLAKAEEIINLIKKYYDIGIDIVKIRFHRITINDDLNIVKEIRKEFGEQIK
EEEEEECCCCCHHHHHHHHHHHHHHHCCEEEEEEEEEEEEECCCHHHHHHHHHHCCCEEE
IAVDANQAWGLVSPFWNRYDALKVAKELERYNIEWLEEPLFKDDIEGYAWLRRNTSVKIA
EEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHCCCCEEEEECCCEEEEE
AGELEHNFWIFKKFIEENALDIVQADAIYSNGISECIKIALMAQVNGLLFLPHAWDPGLG
ECCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEECCCCCCCCC
WLANLHLTASLPESLTPYLETPLDPLWWFNDVIQFAFKEGIEIQNGYVRVPEKEGLGIEL
CEEEEEEEECCCCCCCHHHHCCCCHHHHHHHHHHHHHHCCCEECCCEEECCCCCCCEEEE
DMEKIKKFIT
CHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-chloro-2,5-dihydro-5-oxofuran-2-acetate

Specific reaction: 2-chloro-2,5-dihydro-5-oxofuran-2-acetate = 3-chloro-cis,cis-muconate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353932; 9384377 [H]