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The map label for this gene is acsA [H]

Identifier: 227827023

GI number: 227827023

Start: 633970

End: 635838

Strand: Direct

Name: acsA [H]

Synonym: M1425_0681

Alternate gene names: 227827023

Gene position: 633970-635838 (Clockwise)

Preceding gene: 227827017

Following gene: 227827025

Centisome position: 24.3

GC content: 40.29

Gene sequence:

>1869_bases
ATGAGTTGGAAAATATATTCTCTAGATCAGATTAAAGCATTGGCGAAATTCTCTATGGAAAATCCAGAAGCCTTCTGGCG
AGATAAGGCTAATTACATAACTTGGTTTAGGCAACCAGAGAAAATACTTGAGGGAGAGCCACCGTACGAGAAGTGGTTCG
TTGGAGGAACCACAAATATATCCTATAACGCTATAGATAGGCATTTACCAGATAAGAAAGATAAGGTGGCATTTTATTGG
ACCAATGAAAGATTAGATAGTTTTAGGTCAATATCCTTCCAAGATTTATATCAAGAAGTGAACAAAGCTGCTTATGTCTT
AAGTGAATTGGGCGTGAAAAAAGGCGATACGGTATCCATGCTAATGCCTAATATACCAGAAGCTGTATACTTCTCCTTAG
CAACACACAGACTGGGGGCAATTCTAATAATACATTATGTAGGTTTGAGCGAGGAAACAGTGGCATATAGGTTTAATGAT
TGCGGTTCAAAAGTATTGATTGTTGCTTCAAAGACGTTTAGGAATGGAAATGAGATAAGAATTAAGGATTTTGTGGATAA
AGTTTTGGAATCTCATACTACACCCATACAGAAAGTCTTGGCAGTGCCGAGGGGATATTCAGATTTTAATGTTAATGGGA
AAAGGGATGTTGTGTATGATGATGTGAAACCTAAAGGGAAAGTTTACGTTAAACCAGTGGAAGTTGAGGCTAACGAGCCA
GCGACAGTTTATTACACGTCTGGAACTACGGGAAGACCTAAGGGATTATACCATAGTAATGGAGGGTATGTGATAGCACT
CAATTGGGCTTTTAAGGCAATATTTAACCCAACAGAGAATGACGTTTGGTGGACTGTTTCAGAATTAGGTTGGCCAGTAT
GGCCAATGGCAAATTTATACACGATACCAGTAATGGGAATACCTGGTGTGCTTTTTGAGGGATATGTTGGTTATAAAAGG
GATCTATTCTCGAGAGTAATTGAGAGATATAATGTAAGCCTAGTTTGGAGTTCTACCACAACTCTTTACACTCTCAAGAG
TTTAGGAGAGGAGTCAGTTAAGAGCGGAGATACATCAAGCTTAAGAATGATTCTTAATACAGGAGAACCTTTAAACGTTG
GAGCGTGGATGTGGTTAAATCAGAATCTTCCTCAAGTTAAGATAGCAGATGCGTACTGGATGACCGAGCATTTATCGCCA
GTTGGTGCAACTCCTTACGGAATCGGCGAAATTCCCTTTAAGGCAGGTTCCGCTGGTATACAATTCCCAGGAGGAACATA
TTTCTTAATTGTTGACGATGAGGGAAAACCATTACCTCCTAAGCAAAAGGGTTATATAGTGTTAAAACCGCTTAACCCAG
CCTTAGCTAAAATGTGGAATGATCCCAATTACGAGAAGATAAAGGAGAAGTATTGGTCTAGGTTCCCAGGATACTTCTAC
ACTGGAGATTACGGTTATGTTGATGAGGATGGATATCTGTTCGTGCTGGGAAGAGCTGATGATGTAATAAGAGCTGAAGG
GGAGAGGATAGGAACACTTGAAGTTGAAAGTGTAATAGTAACTCATCCCAACGTGGCCGAAGCTGCCGTAATAGGTCAAG
GCGGTAACATAATAGCGTTTGTAGTCCCAAGACAAGGGGTTGAGGTTGGTGACGCACTCAGAAATGACATTAAGAACTAT
TGTAGGAACGCTGGTTACATAGTTGATAAAGTAGTGTTTGTGAAGAGACTGCCTAAAACCAAAAGCGGGAAGATAATGAG
AAGGCTACTAAAGGCTATCCTAGCTAACGAGAATCCAGGTGATGTATCAACATTGGACGATATAAGGATAATGGAGGAAC
TTAAGGAAGCGTTGAAGAGCCTAAGCTAA

Upstream 100 bases:

>100_bases
ATATTTATCCTACATTTAATAACTTTTAAATCTTATATCAATCTATCATTATTGAACGAAAGGTATATATAATTTTTAAT
TTACAAATGTATATGATAAC

Downstream 100 bases:

>100_bases
AATGTTTTTTATGGAAAACGCAATTAACTTCCTTTGGGCATTTCTCAAATGATAGAGGAACGTTACTTTACTTATTCCTA
GTAGCTTAGCTAATTCATCG

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Acetate--CoA ligase; Acyl-activating enzyme [H]

Number of amino acids: Translated: 622; Mature: 621

Protein sequence:

>622_residues
MSWKIYSLDQIKALAKFSMENPEAFWRDKANYITWFRQPEKILEGEPPYEKWFVGGTTNISYNAIDRHLPDKKDKVAFYW
TNERLDSFRSISFQDLYQEVNKAAYVLSELGVKKGDTVSMLMPNIPEAVYFSLATHRLGAILIIHYVGLSEETVAYRFND
CGSKVLIVASKTFRNGNEIRIKDFVDKVLESHTTPIQKVLAVPRGYSDFNVNGKRDVVYDDVKPKGKVYVKPVEVEANEP
ATVYYTSGTTGRPKGLYHSNGGYVIALNWAFKAIFNPTENDVWWTVSELGWPVWPMANLYTIPVMGIPGVLFEGYVGYKR
DLFSRVIERYNVSLVWSSTTTLYTLKSLGEESVKSGDTSSLRMILNTGEPLNVGAWMWLNQNLPQVKIADAYWMTEHLSP
VGATPYGIGEIPFKAGSAGIQFPGGTYFLIVDDEGKPLPPKQKGYIVLKPLNPALAKMWNDPNYEKIKEKYWSRFPGYFY
TGDYGYVDEDGYLFVLGRADDVIRAEGERIGTLEVESVIVTHPNVAEAAVIGQGGNIIAFVVPRQGVEVGDALRNDIKNY
CRNAGYIVDKVVFVKRLPKTKSGKIMRRLLKAILANENPGDVSTLDDIRIMEELKEALKSLS

Sequences:

>Translated_622_residues
MSWKIYSLDQIKALAKFSMENPEAFWRDKANYITWFRQPEKILEGEPPYEKWFVGGTTNISYNAIDRHLPDKKDKVAFYW
TNERLDSFRSISFQDLYQEVNKAAYVLSELGVKKGDTVSMLMPNIPEAVYFSLATHRLGAILIIHYVGLSEETVAYRFND
CGSKVLIVASKTFRNGNEIRIKDFVDKVLESHTTPIQKVLAVPRGYSDFNVNGKRDVVYDDVKPKGKVYVKPVEVEANEP
ATVYYTSGTTGRPKGLYHSNGGYVIALNWAFKAIFNPTENDVWWTVSELGWPVWPMANLYTIPVMGIPGVLFEGYVGYKR
DLFSRVIERYNVSLVWSSTTTLYTLKSLGEESVKSGDTSSLRMILNTGEPLNVGAWMWLNQNLPQVKIADAYWMTEHLSP
VGATPYGIGEIPFKAGSAGIQFPGGTYFLIVDDEGKPLPPKQKGYIVLKPLNPALAKMWNDPNYEKIKEKYWSRFPGYFY
TGDYGYVDEDGYLFVLGRADDVIRAEGERIGTLEVESVIVTHPNVAEAAVIGQGGNIIAFVVPRQGVEVGDALRNDIKNY
CRNAGYIVDKVVFVKRLPKTKSGKIMRRLLKAILANENPGDVSTLDDIRIMEELKEALKSLS
>Mature_621_residues
SWKIYSLDQIKALAKFSMENPEAFWRDKANYITWFRQPEKILEGEPPYEKWFVGGTTNISYNAIDRHLPDKKDKVAFYWT
NERLDSFRSISFQDLYQEVNKAAYVLSELGVKKGDTVSMLMPNIPEAVYFSLATHRLGAILIIHYVGLSEETVAYRFNDC
GSKVLIVASKTFRNGNEIRIKDFVDKVLESHTTPIQKVLAVPRGYSDFNVNGKRDVVYDDVKPKGKVYVKPVEVEANEPA
TVYYTSGTTGRPKGLYHSNGGYVIALNWAFKAIFNPTENDVWWTVSELGWPVWPMANLYTIPVMGIPGVLFEGYVGYKRD
LFSRVIERYNVSLVWSSTTTLYTLKSLGEESVKSGDTSSLRMILNTGEPLNVGAWMWLNQNLPQVKIADAYWMTEHLSPV
GATPYGIGEIPFKAGSAGIQFPGGTYFLIVDDEGKPLPPKQKGYIVLKPLNPALAKMWNDPNYEKIKEKYWSRFPGYFYT
GDYGYVDEDGYLFVLGRADDVIRAEGERIGTLEVESVIVTHPNVAEAAVIGQGGNIIAFVVPRQGVEVGDALRNDIKNYC
RNAGYIVDKVVFVKRLPKTKSGKIMRRLLKAILANENPGDVSTLDDIRIMEELKEALKSLS

Specific function: Enables The Cell To Use Acetate During Aerobic Growth To Generate Energy Via The Tca Cycle, And Biosynthetic Compounds Via The Glyoxylate Shunt. Acetylates Chey, The Response Regulator Involved In Flagellar Movement And Chemotaxis. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI13375727, Length=623, Percent_Identity=33.2263242375602, Blast_Score=306, Evalue=4e-83,
Organism=Homo sapiens, GI8923896, Length=664, Percent_Identity=31.0240963855422, Blast_Score=292, Evalue=7e-79,
Organism=Homo sapiens, GI253970489, Length=679, Percent_Identity=30.4860088365243, Blast_Score=288, Evalue=7e-78,
Organism=Homo sapiens, GI28416953, Length=628, Percent_Identity=30.7324840764331, Blast_Score=284, Evalue=2e-76,
Organism=Homo sapiens, GI38505220, Length=539, Percent_Identity=28.9424860853432, Blast_Score=160, Evalue=3e-39,
Organism=Homo sapiens, GI122937307, Length=537, Percent_Identity=27.7467411545624, Blast_Score=159, Evalue=9e-39,
Organism=Homo sapiens, GI42544132, Length=529, Percent_Identity=28.3553875236295, Blast_Score=153, Evalue=5e-37,
Organism=Homo sapiens, GI31982927, Length=645, Percent_Identity=25.1162790697674, Blast_Score=150, Evalue=4e-36,
Organism=Homo sapiens, GI115511026, Length=539, Percent_Identity=27.6437847866419, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI157311624, Length=545, Percent_Identity=26.4220183486239, Blast_Score=126, Evalue=7e-29,
Organism=Homo sapiens, GI157311622, Length=545, Percent_Identity=26.4220183486239, Blast_Score=126, Evalue=7e-29,
Organism=Homo sapiens, GI58082049, Length=548, Percent_Identity=26.2773722627737, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI227499619, Length=470, Percent_Identity=24.468085106383, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1790505, Length=632, Percent_Identity=32.753164556962, Blast_Score=342, Evalue=6e-95,
Organism=Escherichia coli, GI1786529, Length=626, Percent_Identity=33.0670926517572, Blast_Score=322, Evalue=6e-89,
Organism=Escherichia coli, GI1788107, Length=523, Percent_Identity=26.9598470363289, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI221142682, Length=520, Percent_Identity=24.2307692307692, Blast_Score=112, Evalue=8e-26,
Organism=Escherichia coli, GI145693145, Length=536, Percent_Identity=22.9477611940299, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1786810, Length=539, Percent_Identity=23.0055658627087, Blast_Score=72, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71983001, Length=652, Percent_Identity=30.521472392638, Blast_Score=260, Evalue=2e-69,
Organism=Caenorhabditis elegans, GI71982997, Length=652, Percent_Identity=30.521472392638, Blast_Score=259, Evalue=2e-69,
Organism=Caenorhabditis elegans, GI17558820, Length=535, Percent_Identity=26.5420560747664, Blast_Score=112, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17531443, Length=553, Percent_Identity=24.5931283905967, Blast_Score=111, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17559526, Length=552, Percent_Identity=24.4565217391304, Blast_Score=108, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17538037, Length=561, Percent_Identity=24.9554367201426, Blast_Score=107, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17569507, Length=626, Percent_Identity=22.2044728434505, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17557194, Length=523, Percent_Identity=24.4741873804971, Blast_Score=102, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI32563687, Length=543, Percent_Identity=24.4935543278085, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17560140, Length=524, Percent_Identity=22.9007633587786, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71985884, Length=569, Percent_Identity=22.8471001757469, Blast_Score=87, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6319264, Length=647, Percent_Identity=32.4574961360124, Blast_Score=319, Evalue=7e-88,
Organism=Saccharomyces cerevisiae, GI6323182, Length=649, Percent_Identity=31.2788906009245, Blast_Score=297, Evalue=3e-81,
Organism=Saccharomyces cerevisiae, GI6319699, Length=535, Percent_Identity=25.981308411215, Blast_Score=104, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24667955, Length=647, Percent_Identity=31.5301391035549, Blast_Score=308, Evalue=6e-84,
Organism=Drosophila melanogaster, GI62472339, Length=565, Percent_Identity=32.212389380531, Blast_Score=275, Evalue=7e-74,
Organism=Drosophila melanogaster, GI21355225, Length=629, Percent_Identity=30.8426073131955, Blast_Score=272, Evalue=5e-73,
Organism=Drosophila melanogaster, GI24667959, Length=520, Percent_Identity=31.9230769230769, Blast_Score=246, Evalue=2e-65,
Organism=Drosophila melanogaster, GI21355181, Length=522, Percent_Identity=24.1379310344828, Blast_Score=110, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24648260, Length=520, Percent_Identity=25.1923076923077, Blast_Score=100, Evalue=6e-21,
Organism=Drosophila melanogaster, GI18859661, Length=509, Percent_Identity=24.3614931237721, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24656500, Length=505, Percent_Identity=24.1584158415842, Blast_Score=94, Evalue=4e-19,
Organism=Drosophila melanogaster, GI281365686, Length=312, Percent_Identity=26.2820512820513, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI21356441, Length=398, Percent_Identity=24.1206030150754, Blast_Score=87, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24648253, Length=376, Percent_Identity=24.2021276595745, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21358303, Length=474, Percent_Identity=23.6286919831224, Blast_Score=85, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24648255, Length=376, Percent_Identity=24.2021276595745, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24648257, Length=361, Percent_Identity=25.207756232687, Blast_Score=76, Evalue=6e-14,
Organism=Drosophila melanogaster, GI161076582, Length=386, Percent_Identity=25.1295336787565, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI21356947, Length=387, Percent_Identity=25.8397932816537, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24653035, Length=228, Percent_Identity=27.1929824561404, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011904
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.1 [H]

Molecular weight: Translated: 70160; Mature: 70029

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSWKIYSLDQIKALAKFSMENPEAFWRDKANYITWFRQPEKILEGEPPYEKWFVGGTTNI
CCEEEECHHHHHHHHHHCCCCCHHHHCCCCCEEEEECCCHHHHCCCCCHHHEEECCCCCC
SYNAIDRHLPDKKDKVAFYWTNERLDSFRSISFQDLYQEVNKAAYVLSELGVKKGDTVSM
EECHHHHCCCCCCCCEEEEEECHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEE
LMPNIPEAVYFSLATHRLGAILIIHYVGLSEETVAYRFNDCGSKVLIVASKTFRNGNEIR
ECCCCCHHHHHHHHHHHHCEEEEEEEECCCCCEEEEEECCCCCEEEEEEEECCCCCCEEE
IKDFVDKVLESHTTPIQKVLAVPRGYSDFNVNGKRDVVYDDVKPKGKVYVKPVEVEANEP
HHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCEEEEEEEEECCCC
ATVYYTSGTTGRPKGLYHSNGGYVIALNWAFKAIFNPTENDVWWTVSELGWPVWPMANLY
EEEEEECCCCCCCCCCEECCCCEEEEEEEEEEEECCCCCCCEEEEHHHCCCCCCCCCCEE
TIPVMGIPGVLFEGYVGYKRDLFSRVIERYNVSLVWSSTTTLYTLKSLGEESVKSGDTSS
EEECCCCCHHHHHCCCCHHHHHHHHHHHHCCEEEEEECCCHHHHHHHCCHHHHCCCCCCE
LRMILNTGEPLNVGAWMWLNQNLPQVKIADAYWMTEHLSPVGATPYGIGEIPFKAGSAGI
EEEEEECCCCCCCCEEEEECCCCCEEEEEHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCE
QFPGGTYFLIVDDEGKPLPPKQKGYIVLKPLNPALAKMWNDPNYEKIKEKYWSRFPGYFY
ECCCCEEEEEECCCCCCCCCCCCCEEEEECCCHHHHHHCCCCCHHHHHHHHHHHCCCCEE
TGDYGYVDEDGYLFVLGRADDVIRAEGERIGTLEVESVIVTHPNVAEAAVIGQGGNIIAF
ECCCCEECCCCEEEEEECCCCHHHCCCCEEEEEEEEEEEEECCCCCCEEEEECCCCEEEE
VVPRQGVEVGDALRNDIKNYCRNAGYIVDKVVFVKRLPKTKSGKIMRRLLKAILANENPG
EECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCC
DVSTLDDIRIMEELKEALKSLS
CCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SWKIYSLDQIKALAKFSMENPEAFWRDKANYITWFRQPEKILEGEPPYEKWFVGGTTNI
CEEEECHHHHHHHHHHCCCCCHHHHCCCCCEEEEECCCHHHHCCCCCHHHEEECCCCCC
SYNAIDRHLPDKKDKVAFYWTNERLDSFRSISFQDLYQEVNKAAYVLSELGVKKGDTVSM
EECHHHHCCCCCCCCEEEEEECHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEE
LMPNIPEAVYFSLATHRLGAILIIHYVGLSEETVAYRFNDCGSKVLIVASKTFRNGNEIR
ECCCCCHHHHHHHHHHHHCEEEEEEEECCCCCEEEEEECCCCCEEEEEEEECCCCCCEEE
IKDFVDKVLESHTTPIQKVLAVPRGYSDFNVNGKRDVVYDDVKPKGKVYVKPVEVEANEP
HHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCEEEEEEEEECCCC
ATVYYTSGTTGRPKGLYHSNGGYVIALNWAFKAIFNPTENDVWWTVSELGWPVWPMANLY
EEEEEECCCCCCCCCCEECCCCEEEEEEEEEEEECCCCCCCEEEEHHHCCCCCCCCCCEE
TIPVMGIPGVLFEGYVGYKRDLFSRVIERYNVSLVWSSTTTLYTLKSLGEESVKSGDTSS
EEECCCCCHHHHHCCCCHHHHHHHHHHHHCCEEEEEECCCHHHHHHHCCHHHHCCCCCCE
LRMILNTGEPLNVGAWMWLNQNLPQVKIADAYWMTEHLSPVGATPYGIGEIPFKAGSAGI
EEEEEECCCCCCCCEEEEECCCCCEEEEEHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCE
QFPGGTYFLIVDDEGKPLPPKQKGYIVLKPLNPALAKMWNDPNYEKIKEKYWSRFPGYFY
ECCCCEEEEEECCCCCCCCCCCCCEEEEECCCHHHHHHCCCCCHHHHHHHHHHHCCCCEE
TGDYGYVDEDGYLFVLGRADDVIRAEGERIGTLEVESVIVTHPNVAEAAVIGQGGNIIAF
ECCCCEECCCCEEEEEECCCCHHHCCCCEEEEEEEEEEEEECCCCCCEEEEECCCCEEEE
VVPRQGVEVGDALRNDIKNYCRNAGYIVDKVVFVKRLPKTKSGKIMRRLLKAILANENPG
EECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCC
DVSTLDDIRIMEELKEALKSLS
CCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11792869 [H]