Click here to switch to the map view.

The map label for this gene is gloB [H]

Identifier: 227827011

GI number: 227827011

Start: 622197

End: 622940

Strand: Direct

Name: gloB [H]

Synonym: M1425_0669

Alternate gene names: 227827011

Gene position: 622197-622940 (Clockwise)

Preceding gene: 227827010

Following gene: 227827012

Centisome position: 23.85

GC content: 36.56

Gene sequence:

>744_bases
ATGATGATATTCAGGCAAATTATAAGCAAAAGTGGTGGTTGTGCTACTTATATCTTTGGCTGTACTCAAGCTGGAGAATT
GTTTGTAGTCGATCCTAGGTACGAGATGGTAGATGAAATAGTTAGATTAGCTCAAGACTTAGGTAATATGAAGATAGCTT
ATATAATAGACACACACACTCACGCTGATCACTTATCTGGTGTAAAGAAATTGCAAGCATTAACTAACGCTAATATTTAC
TATCATGAGGAGTCACAAGTCAAATTTAAGGTAGAGAGGATTAAGGATGGAGAGGAGATAAAGGCAGGAAATGTGAAGAT
AAAGGTAATTCATACTCCTGGACATACTCCAGATAGCATTTCAGTTCTAGTATATGATAAAAGGAGGGATGACAGTTGGA
ATGAGCCGTGGGCTGTATTAACTGGGGATACTTTGTTCGTAGGAGGTGTTGGAAGAATTGATATAGGTGGGGAAAATGCG
GAGGAGAATTTATACTATAGTCTAGCCAAGCTGAAAGGATTGCCAGATTATGTTGAAATTTACCCAACACATACTGCCGG
TTCAGTATGTGGTGTTGGGATAAGTGGAAAACCTAGTTCCACAATAGGCTTTGAAAAGAGATTCAATACTTTATTTAGAA
TAAACGAAAAAGACGAGTTCATAAATAGAGTTAGAGAAGTTAAAATTTCTAAGCCCAAAGAGTTTGATGAGTATATAAGG
AAGAATTTAGAAGGTGTAATTTGA

Upstream 100 bases:

>100_bases
GGATTCATGTAGAGGATGTGTACTCCAAGCGGAAGAGCACTTCGAGTTATTAACTTATAAGAGTGAATAACTCTTATATC
TAATTATTTATTTAAAGTAA

Downstream 100 bases:

>100_bases
GAGGAAAATATTTTAAAATCATTAAAAATATTTTTTAAATTATACTTATAACAGCACCTTTTGCCGGTCCGGTATCATTT
CCTCTTCTTGTAATAAAGGC

Product: beta-lactamase

Products: NA

Alternate protein names: Glyoxalase II; Glx II [H]

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MMIFRQIISKSGGCATYIFGCTQAGELFVVDPRYEMVDEIVRLAQDLGNMKIAYIIDTHTHADHLSGVKKLQALTNANIY
YHEESQVKFKVERIKDGEEIKAGNVKIKVIHTPGHTPDSISVLVYDKRRDDSWNEPWAVLTGDTLFVGGVGRIDIGGENA
EENLYYSLAKLKGLPDYVEIYPTHTAGSVCGVGISGKPSSTIGFEKRFNTLFRINEKDEFINRVREVKISKPKEFDEYIR
KNLEGVI

Sequences:

>Translated_247_residues
MMIFRQIISKSGGCATYIFGCTQAGELFVVDPRYEMVDEIVRLAQDLGNMKIAYIIDTHTHADHLSGVKKLQALTNANIY
YHEESQVKFKVERIKDGEEIKAGNVKIKVIHTPGHTPDSISVLVYDKRRDDSWNEPWAVLTGDTLFVGGVGRIDIGGENA
EENLYYSLAKLKGLPDYVEIYPTHTAGSVCGVGISGKPSSTIGFEKRFNTLFRINEKDEFINRVREVKISKPKEFDEYIR
KNLEGVI
>Mature_247_residues
MMIFRQIISKSGGCATYIFGCTQAGELFVVDPRYEMVDEIVRLAQDLGNMKIAYIIDTHTHADHLSGVKKLQALTNANIY
YHEESQVKFKVERIKDGEEIKAGNVKIKVIHTPGHTPDSISVLVYDKRRDDSWNEPWAVLTGDTLFVGGVGRIDIGGENA
EENLYYSLAKLKGLPDYVEIYPTHTAGSVCGVGISGKPSSTIGFEKRFNTLFRINEKDEFINRVREVKISKPKEFDEYIR
KNLEGVI

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid [H]

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family [H]

Homologues:

Organism=Homo sapiens, GI41327741, Length=246, Percent_Identity=26.0162601626016, Blast_Score=92, Evalue=6e-19,
Organism=Escherichia coli, GI1787158, Length=229, Percent_Identity=26.6375545851528, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17538952, Length=241, Percent_Identity=26.9709543568465, Blast_Score=98, Evalue=5e-21,
Organism=Drosophila melanogaster, GI221330176, Length=244, Percent_Identity=28.6885245901639, Blast_Score=101, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR017782 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.1.2.6 [H]

Molecular weight: Translated: 27821; Mature: 27821

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMIFRQIISKSGGCATYIFGCTQAGELFVVDPRYEMVDEIVRLAQDLGNMKIAYIIDTHT
CHHHHHHHCCCCCEEEEEEEECCCCCEEEECCCHHHHHHHHHHHHHHCCEEEEEEEECCC
HADHLSGVKKLQALTNANIYYHEESQVKFKVERIKDGEEIKAGNVKIKVIHTPGHTPDSI
CHHHHHHHHHHHHHHCCEEEEECCCCEEEEEEECCCCCEEEECCEEEEEEECCCCCCCCE
SVLVYDKRRDDSWNEPWAVLTGDTLFVGGVGRIDIGGENAEENLYYSLAKLKGLPDYVEI
EEEEEECCCCCCCCCCEEEEECCEEEECCCEEEEECCCCCCHHHHHHHHHHCCCCCCEEE
YPTHTAGSVCGVGISGKPSSTIGFEKRFNTLFRINEKDEFINRVREVKISKPKEFDEYIR
EECCCCCCEEECCCCCCCCCCCCHHHHCCEEEEECCHHHHHHHHHHCCCCCCHHHHHHHH
KNLEGVI
HHHCCCC
>Mature Secondary Structure
MMIFRQIISKSGGCATYIFGCTQAGELFVVDPRYEMVDEIVRLAQDLGNMKIAYIIDTHT
CHHHHHHHCCCCCEEEEEEEECCCCCEEEECCCHHHHHHHHHHHHHHCCEEEEEEEECCC
HADHLSGVKKLQALTNANIYYHEESQVKFKVERIKDGEEIKAGNVKIKVIHTPGHTPDSI
CHHHHHHHHHHHHHHCCEEEEECCCCEEEEEEECCCCCEEEECCEEEEEEECCCCCCCCE
SVLVYDKRRDDSWNEPWAVLTGDTLFVGGVGRIDIGGENAEENLYYSLAKLKGLPDYVEI
EEEEEECCCCCCCCCCEEEEECCEEEECCCEEEEECCCCCCHHHHHHHHHHCCCCCCEEE
YPTHTAGSVCGVGISGKPSSTIGFEKRFNTLFRINEKDEFINRVREVKISKPKEFDEYIR
EECCCCCCEEECCCCCCCCCCCCHHHHCCEEEEECCHHHHHHHHHHCCCCCCHHHHHHHH
KNLEGVI
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA