Click here to switch to the map view.

The map label for this gene is mcrB [C]

Identifier: 227826951

GI number: 227826951

Start: 544645

End: 546402

Strand: Direct

Name: mcrB [C]

Synonym: M1425_0605

Alternate gene names: 227826951

Gene position: 544645-546402 (Clockwise)

Preceding gene: 227826950

Following gene: 227826952

Centisome position: 20.88

GC content: 30.83

Gene sequence:

>1758_bases
TTGCACGACAATAATAGATTCATCAAAAACGAGGAAAATTTACATATTATTGATAAAATTTTGGGAAAAAAATTTAAGAC
TTCCTTATCAGTTTATCTTATGTATGGTGACTGGTTAGTAGTTAGGCCTCAATTTAGTAAACTTTTTAACACTCCAGACA
TAGCTATAGAGACTTTCTTAAATTACATTCAAAATGATAAATTTCTTCCTTGCTCATTTATAGGCTCTAAAGAGCATTGG
ATAGAAGCAATAAAATACTCGATTAAAGGAGAAATAGGTTACACGCTATGGGGTTCAGGAGATATGAGTTCTTCTACTGG
GATAAATAATAGATATCAAGAAGCTTATATCAAAAAAGATGGAAATAGAATATTACCTTCAGATGATAGAATAATGGTAG
CTGTATTAAGAGTTTCAGGATTAGGCATAATAGGTTTTGGATTAGTGACTGACGTGGAAACTGATGCAATTAGGAGTTTT
ATAGGTTGGCATGAAGATGATCGATATTGGATTACAAGATTTAGGATAAAGATATTTTGGTTACATGAAAGTATAAGAAA
TAATCCTACAGACTATGATAGATGGCAAGGTGATAATATAGAACTAGAAGGTACTAATACTCAAACTAATAATTGCTATA
CTAAGGACAACCATAAAGCAATAAACAGCGTTAAGAGATTTATTTTATCTAAAAAAGATGAAATAAAGCCTACGTTAGAA
TTTTATATAAACTTATTACGGAGTGAACCTCAGATTAAGGAAGAAGCTATTACGATTTCTTCTAAAATAAATTGCAAAAA
TTCTAATAACGACGTAAAAGTGGAAGATGTATATTTGCCTCAAGGTATAACTGGCATTATAATAAAATCCTTAGAAAAGG
GCAATGTCCTCTTTATTGGTCCACCTGGTGTTGGTAAAACTAAATTAGCTTTAGAGCTCGTAAAGTCATTAAGCGGGAAC
GACGAGTGCTTTAATATAGTTACTGCAAATTCCTTGTGGTTTAGAAGGACTTTAATAGGTGGTGAGAGCATTAGGGAAGG
TTCAGTAATATGGAAAAGCGGTCTGCTAATTCAAGCTTATGTTAAGGCTTCAAAGGTAAATTATGGTAATTATTATATTA
TCATTGATGAGATAAATAGAGCTGACGTAGATAAAGCGTTTGGGGAGTTATTTACAATATTCTCCAGTAGTAATCCCAAG
GATTGGTTCATTCCTAGGGCTTTAATTGACGAGATAAAAAGCTACGGAGATAAAGTAGACGAATATACAAAGGAGTTCTT
TAATATTTATGAAGATTTAAGAAAGCAAGGTAAGGAAAACGAGCCGTTAAAAAAGATTAGAATTATTGCCACGTTAAATT
TGACAGACGCAAGGAACTTATTTTACGTTGGAGACGCGTTAACTAGAAGGTTCACGAGCTTTTACTTCGATTATCCTAAA
GGCACTGAAGATTTGGATAAATTCCTTAGAAATTATAATTTAAGAGAAGAAGAAAAAGAAGAAATAAGGGATCTTGTAAA
ATATTTAAGGGAAAAACTTGAGGAAAAAGATAAGATTATTAAGTTTAATATATCTCCTGCAAGCTTAAAATCTGCATTAG
AAATTTACTCATCATTCGATAATAGGAATATAGACAATTTTATTCTTATTCTAAAGAGTACATTAGGAACTTTAAATCAA
GATAAAATAGATAAAGTAAATAAACTGATAGATAACTGGAAGGAAATAAAAGAAAAAGAAAATAATGAAGAAAAATAG

Upstream 100 bases:

>100_bases
TAGCGTAAGACTTATACTTGTAAATACGATTCGAAGATTATGTAACTAAAAAATTTGAAAATAGGGATAGCGAATTTTTT
ACCATGATTCTAGATTTGAT

Downstream 100 bases:

>100_bases
TATTATCTTTTATTATCATTATCATTTATCTCTTCAATTTCTTCGGGCTTTACATTTGCCTCTTTTAGTATTTCGTCCAT
AAGTTCCTTTGAAGGGAACG

Product: ATPase AAA

Products: NA

Alternate protein names: 5-Methylcytosine-Specific Restriction; AAA ATPase Central Domain-Containing Protein; Restriction Endonuclease Related Protein

Number of amino acids: Translated: 585; Mature: 585

Protein sequence:

>585_residues
MHDNNRFIKNEENLHIIDKILGKKFKTSLSVYLMYGDWLVVRPQFSKLFNTPDIAIETFLNYIQNDKFLPCSFIGSKEHW
IEAIKYSIKGEIGYTLWGSGDMSSSTGINNRYQEAYIKKDGNRILPSDDRIMVAVLRVSGLGIIGFGLVTDVETDAIRSF
IGWHEDDRYWITRFRIKIFWLHESIRNNPTDYDRWQGDNIELEGTNTQTNNCYTKDNHKAINSVKRFILSKKDEIKPTLE
FYINLLRSEPQIKEEAITISSKINCKNSNNDVKVEDVYLPQGITGIIIKSLEKGNVLFIGPPGVGKTKLALELVKSLSGN
DECFNIVTANSLWFRRTLIGGESIREGSVIWKSGLLIQAYVKASKVNYGNYYIIIDEINRADVDKAFGELFTIFSSSNPK
DWFIPRALIDEIKSYGDKVDEYTKEFFNIYEDLRKQGKENEPLKKIRIIATLNLTDARNLFYVGDALTRRFTSFYFDYPK
GTEDLDKFLRNYNLREEEKEEIRDLVKYLREKLEEKDKIIKFNISPASLKSALEIYSSFDNRNIDNFILILKSTLGTLNQ
DKIDKVNKLIDNWKEIKEKENNEEK

Sequences:

>Translated_585_residues
MHDNNRFIKNEENLHIIDKILGKKFKTSLSVYLMYGDWLVVRPQFSKLFNTPDIAIETFLNYIQNDKFLPCSFIGSKEHW
IEAIKYSIKGEIGYTLWGSGDMSSSTGINNRYQEAYIKKDGNRILPSDDRIMVAVLRVSGLGIIGFGLVTDVETDAIRSF
IGWHEDDRYWITRFRIKIFWLHESIRNNPTDYDRWQGDNIELEGTNTQTNNCYTKDNHKAINSVKRFILSKKDEIKPTLE
FYINLLRSEPQIKEEAITISSKINCKNSNNDVKVEDVYLPQGITGIIIKSLEKGNVLFIGPPGVGKTKLALELVKSLSGN
DECFNIVTANSLWFRRTLIGGESIREGSVIWKSGLLIQAYVKASKVNYGNYYIIIDEINRADVDKAFGELFTIFSSSNPK
DWFIPRALIDEIKSYGDKVDEYTKEFFNIYEDLRKQGKENEPLKKIRIIATLNLTDARNLFYVGDALTRRFTSFYFDYPK
GTEDLDKFLRNYNLREEEKEEIRDLVKYLREKLEEKDKIIKFNISPASLKSALEIYSSFDNRNIDNFILILKSTLGTLNQ
DKIDKVNKLIDNWKEIKEKENNEEK
>Mature_585_residues
MHDNNRFIKNEENLHIIDKILGKKFKTSLSVYLMYGDWLVVRPQFSKLFNTPDIAIETFLNYIQNDKFLPCSFIGSKEHW
IEAIKYSIKGEIGYTLWGSGDMSSSTGINNRYQEAYIKKDGNRILPSDDRIMVAVLRVSGLGIIGFGLVTDVETDAIRSF
IGWHEDDRYWITRFRIKIFWLHESIRNNPTDYDRWQGDNIELEGTNTQTNNCYTKDNHKAINSVKRFILSKKDEIKPTLE
FYINLLRSEPQIKEEAITISSKINCKNSNNDVKVEDVYLPQGITGIIIKSLEKGNVLFIGPPGVGKTKLALELVKSLSGN
DECFNIVTANSLWFRRTLIGGESIREGSVIWKSGLLIQAYVKASKVNYGNYYIIIDEINRADVDKAFGELFTIFSSSNPK
DWFIPRALIDEIKSYGDKVDEYTKEFFNIYEDLRKQGKENEPLKKIRIIATLNLTDARNLFYVGDALTRRFTSFYFDYPK
GTEDLDKFLRNYNLREEEKEEIRDLVKYLREKLEEKDKIIKFNISPASLKSALEIYSSFDNRNIDNFILILKSTLGTLNQ
DKIDKVNKLIDNWKEIKEKENNEEK

Specific function: Recognizes N4- And C5-Methylcytosine (And 5-Hydroxy- Methylcytosines) Produced By A Broad Range Of DNA Methylases And Appears To Act Against 5-Methylcytosine Preceded By A Purine Residue. Binds To DNA Containing Methylated Cytosines; Also Binds To GTP. [

COG id: COG1401

COG function: function code V; GTPase subunit of restriction endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082429, Length=284, Percent_Identity=26.056338028169, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.1.21.- [C]

Molecular weight: Translated: 67938; Mature: 67938

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHDNNRFIKNEENLHIIDKILGKKFKTSLSVYLMYGDWLVVRPQFSKLFNTPDIAIETFL
CCCCCCCCCCCCCEEHHHHHHCHHHCCCEEEEEEECCEEEECCCHHHHCCCCCHHHHHHH
NYIQNDKFLPCSFIGSKEHWIEAIKYSIKGEIGYTLWGSGDMSSSTGINNRYQEAYIKKD
HHHCCCCCCEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHCC
GNRILPSDDRIMVAVLRVSGLGIIGFGLVTDVETDAIRSFIGWHEDDRYWITRFRIKIFW
CCEECCCCCCEEEEEEECCCCCEEEEHHHCCCCHHHHHHHHCCCCCCCEEEEEEEEEEEE
LHESIRNNPTDYDRWQGDNIELEGTNTQTNNCYTKDNHKAINSVKRFILSKKDEIKPTLE
EEHHHCCCCCCCCCCCCCCEEEECCCCCCCCCEECCCHHHHHHHHHHHHCCCCCCCHHHH
FYINLLRSEPQIKEEAITISSKINCKNSNNDVKVEDVYLPQGITGIIIKSLEKGNVLFIG
HHHHHHHCCCCHHHHHEEEEEEEECCCCCCCEEEEEEECCCCCHHHHHHHCCCCCEEEEC
PPGVGKTKLALELVKSLSGNDECFNIVTANSLWFRRTLIGGESIREGSVIWKSGLLIQAY
CCCCCHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHCCCCCCCCCCEEEECCCEEEEE
VKASKVNYGNYYIIIDEINRADVDKAFGELFTIFSSSNPKDWFIPRALIDEIKSYGDKVD
HHHHEECCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
EYTKEFFNIYEDLRKQGKENEPLKKIRIIATLNLTDARNLFYVGDALTRRFTSFYFDYPK
HHHHHHHHHHHHHHHCCCCCCCHHEEEEEEEEECCCCCCEEEECHHHHHHHHHHHCCCCC
GTEDLDKFLRNYNLREEEKEEIRDLVKYLREKLEEKDKIIKFNISPASLKSALEIYSSFD
CHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHCC
NRNIDNFILILKSTLGTLNQDKIDKVNKLIDNWKEIKEKENNEEK
CCCCCHHEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MHDNNRFIKNEENLHIIDKILGKKFKTSLSVYLMYGDWLVVRPQFSKLFNTPDIAIETFL
CCCCCCCCCCCCCEEHHHHHHCHHHCCCEEEEEEECCEEEECCCHHHHCCCCCHHHHHHH
NYIQNDKFLPCSFIGSKEHWIEAIKYSIKGEIGYTLWGSGDMSSSTGINNRYQEAYIKKD
HHHCCCCCCEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHCC
GNRILPSDDRIMVAVLRVSGLGIIGFGLVTDVETDAIRSFIGWHEDDRYWITRFRIKIFW
CCEECCCCCCEEEEEEECCCCCEEEEHHHCCCCHHHHHHHHCCCCCCCEEEEEEEEEEEE
LHESIRNNPTDYDRWQGDNIELEGTNTQTNNCYTKDNHKAINSVKRFILSKKDEIKPTLE
EEHHHCCCCCCCCCCCCCCEEEECCCCCCCCCEECCCHHHHHHHHHHHHCCCCCCCHHHH
FYINLLRSEPQIKEEAITISSKINCKNSNNDVKVEDVYLPQGITGIIIKSLEKGNVLFIG
HHHHHHHCCCCHHHHHEEEEEEEECCCCCCCEEEEEEECCCCCHHHHHHHCCCCCEEEEC
PPGVGKTKLALELVKSLSGNDECFNIVTANSLWFRRTLIGGESIREGSVIWKSGLLIQAY
CCCCCHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHCCCCCCCCCCEEEECCCEEEEE
VKASKVNYGNYYIIIDEINRADVDKAFGELFTIFSSSNPKDWFIPRALIDEIKSYGDKVD
HHHHEECCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
EYTKEFFNIYEDLRKQGKENEPLKKIRIIATLNLTDARNLFYVGDALTRRFTSFYFDYPK
HHHHHHHHHHHHHHHCCCCCCCHHEEEEEEEEECCCCCCEEEECHHHHHHHHHHHCCCCC
GTEDLDKFLRNYNLREEEKEEIRDLVKYLREKLEEKDKIIKFNISPASLKSALEIYSSFD
CHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHCC
NRNIDNFILILKSTLGTLNQDKIDKVNKLIDNWKEIKEKENNEEK
CCCCCHHEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds; Endodeoxyribonucleases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA