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The map label for this gene is proP [H]

Identifier: 227826931

GI number: 227826931

Start: 522425

End: 523717

Strand: Direct

Name: proP [H]

Synonym: M1425_0574

Alternate gene names: 227826931

Gene position: 522425-523717 (Clockwise)

Preceding gene: 227826927

Following gene: 227826932

Centisome position: 20.03

GC content: 38.44

Gene sequence:

>1293_bases
ATGGTTTATGGCTTAAATAAGCAACAATGGTTGGCAATATTCTCTACTTGGCTAGGATGGTTAATGGACGGATACACTAG
TATAGCCTACGCATTAGTTGGAGTTATAATATCAAAAATATTTTTCCCCTCCTCAATTGGAATTTTAGGTCTCATAGCTA
CTTTTGGAGGATTCGCAGTTGGTGCATTAGCAAGGCCAATAGGATCTCTAGTGTTTGGAAACTATATAGGGGATAAGATG
GGTAGAAAAAATATGCTAGTTATAACTATCTTAGGATTTTCGTTGTTGGCTTCTTCAAAATCGCTCTTACCGTCATATGA
GGCTGTAGGTATTTTAGCGCCAATACTCCTATACGTCATATTATTTGCAGAGGGAATGTTTGCCGGGGCCGAATATGGAG
GTGGAACCACACTGGCTCTAGAGTCTGTTCCAGCAGAGAGGAGAGCATTCATAGGCTCATTTGTGCAAAGTGGTTTCGGA
ACGGGATATTTCATCATCTCTCTAGTTTATTCTTTGTTATATAGCATTTATGGGAGTAGCGGTTTCCAGCAAATAGGTTG
GAGAGTACTCTTTGCTACTTGTATAATCCCAGGGTTAATAACATTAATTATTAGGAAGATAACTGACGAAAGTCCAATAT
TTAAGGAAATGGAAAGTAAAAGAGAAGTAGTAAAAATACCTATTAAGAACCTCTTTGCGTCATCCTACTCATCAGTGCTA
ATCGGACTAATGATAACCAGCGGGTTATTGTACATTAATACTGCCACATTCTCTTTCTATCCCTCAGTTCTAACACTCGA
AAATATCCCAGGATCGTTAGTGGGTATAAGCGTTGCTATAATCAACCTAATATCGTTATTTGGAGTGTGGATAGGTGGGT
TTATAGCGGATAAGATTAAGAGGGGAAGAAAGTTCCCAATGTTATTGTATTCAATAATCTTCGTAGCCCTAATATATCCA
ATACTTTATCTAGGACTCTCAAAAAGCTTCTCTTTGGCAACATCAGTATTTAGTATACAAGCCTTCTTGGAAGCGATGAT
ATTCTCAACACTACCAGCGTTTCTTGCAGAACAATTCAGTAAAAAGTACAGGACTACTGGAGTGGGTTTTACGTATAATG
GAGGAGCTATAGCCGGGGGATTTGCAATTTCAGCAATATTTGCTTTATCAACATCCCTAGGATTACTTTACGCTTGGTTC
CTTAACATGATCATAGCAGGGATAATAATGATAATTGGCATAGTCCTTGCGAAAGAGACTTATAGTGGAAAACAAGACCC
TATCATGGAGTGA

Upstream 100 bases:

>100_bases
GGAGATATCCCTTACATAAGTTATAGAAAGTGTTTCCTTCTGGATATAGGAAATTCGACTACCCTATAAGTTAAATATAA
GTTAAGCAATAAAATTATTA

Downstream 100 bases:

>100_bases
GGTGTTTTATTGAAATGAGAATAGGAATAGAGGGAATTAAGGTTGGAGGGTACACAGACGAAAAGGCAAAATCTGGAGTG
ACGGTAATATTAGTAGAAGA

Product: major facilitator superfamily MFS_1

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MVYGLNKQQWLAIFSTWLGWLMDGYTSIAYALVGVIISKIFFPSSIGILGLIATFGGFAVGALARPIGSLVFGNYIGDKM
GRKNMLVITILGFSLLASSKSLLPSYEAVGILAPILLYVILFAEGMFAGAEYGGGTTLALESVPAERRAFIGSFVQSGFG
TGYFIISLVYSLLYSIYGSSGFQQIGWRVLFATCIIPGLITLIIRKITDESPIFKEMESKREVVKIPIKNLFASSYSSVL
IGLMITSGLLYINTATFSFYPSVLTLENIPGSLVGISVAIINLISLFGVWIGGFIADKIKRGRKFPMLLYSIIFVALIYP
ILYLGLSKSFSLATSVFSIQAFLEAMIFSTLPAFLAEQFSKKYRTTGVGFTYNGGAIAGGFAISAIFALSTSLGLLYAWF
LNMIIAGIIMIIGIVLAKETYSGKQDPIME

Sequences:

>Translated_430_residues
MVYGLNKQQWLAIFSTWLGWLMDGYTSIAYALVGVIISKIFFPSSIGILGLIATFGGFAVGALARPIGSLVFGNYIGDKM
GRKNMLVITILGFSLLASSKSLLPSYEAVGILAPILLYVILFAEGMFAGAEYGGGTTLALESVPAERRAFIGSFVQSGFG
TGYFIISLVYSLLYSIYGSSGFQQIGWRVLFATCIIPGLITLIIRKITDESPIFKEMESKREVVKIPIKNLFASSYSSVL
IGLMITSGLLYINTATFSFYPSVLTLENIPGSLVGISVAIINLISLFGVWIGGFIADKIKRGRKFPMLLYSIIFVALIYP
ILYLGLSKSFSLATSVFSIQAFLEAMIFSTLPAFLAEQFSKKYRTTGVGFTYNGGAIAGGFAISAIFALSTSLGLLYAWF
LNMIIAGIIMIIGIVLAKETYSGKQDPIME
>Mature_430_residues
MVYGLNKQQWLAIFSTWLGWLMDGYTSIAYALVGVIISKIFFPSSIGILGLIATFGGFAVGALARPIGSLVFGNYIGDKM
GRKNMLVITILGFSLLASSKSLLPSYEAVGILAPILLYVILFAEGMFAGAEYGGGTTLALESVPAERRAFIGSFVQSGFG
TGYFIISLVYSLLYSIYGSSGFQQIGWRVLFATCIIPGLITLIIRKITDESPIFKEMESKREVVKIPIKNLFASSYSSVL
IGLMITSGLLYINTATFSFYPSVLTLENIPGSLVGISVAIINLISLFGVWIGGFIADKIKRGRKFPMLLYSIIFVALIYP
ILYLGLSKSFSLATSVFSIQAFLEAMIFSTLPAFLAEQFSKKYRTTGVGFTYNGGAIAGGFAISAIFALSTSLGLLYAWF
LNMIIAGIIMIIGIVLAKETYSGKQDPIME

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=426, Percent_Identity=29.1079812206573, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1788292, Length=370, Percent_Identity=27.8378378378378, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1789941, Length=403, Percent_Identity=32.5062034739454, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI87082231, Length=446, Percent_Identity=25.7847533632287, Blast_Score=99, Evalue=8e-22,
Organism=Escherichia coli, GI87082404, Length=377, Percent_Identity=27.8514588859416, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1788942, Length=405, Percent_Identity=26.4197530864197, Blast_Score=90, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46525; Mature: 46525

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVYGLNKQQWLAIFSTWLGWLMDGYTSIAYALVGVIISKIFFPSSIGILGLIATFGGFAV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
GALARPIGSLVFGNYIGDKMGRKNMLVITILGFSLLASSKSLLPSYEAVGILAPILLYVI
HHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHH
LFAEGMFAGAEYGGGTTLALESVPAERRAFIGSFVQSGFGTGYFIISLVYSLLYSIYGSS
HHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC
GFQQIGWRVLFATCIIPGLITLIIRKITDESPIFKEMESKREVVKIPIKNLFASSYSSVL
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGLMITSGLLYINTATFSFYPSVLTLENIPGSLVGISVAIINLISLFGVWIGGFIADKIK
HHHHHHCCCCEEEEHHHHHCCCHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RGRKFPMLLYSIIFVALIYPILYLGLSKSFSLATSVFSIQAFLEAMIFSTLPAFLAEQFS
CCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKYRTTGVGFTYNGGAIAGGFAISAIFALSTSLGLLYAWFLNMIIAGIIMIIGIVLAKET
HHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YSGKQDPIME
CCCCCCCCCC
>Mature Secondary Structure
MVYGLNKQQWLAIFSTWLGWLMDGYTSIAYALVGVIISKIFFPSSIGILGLIATFGGFAV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
GALARPIGSLVFGNYIGDKMGRKNMLVITILGFSLLASSKSLLPSYEAVGILAPILLYVI
HHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHH
LFAEGMFAGAEYGGGTTLALESVPAERRAFIGSFVQSGFGTGYFIISLVYSLLYSIYGSS
HHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC
GFQQIGWRVLFATCIIPGLITLIIRKITDESPIFKEMESKREVVKIPIKNLFASSYSSVL
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGLMITSGLLYINTATFSFYPSVLTLENIPGSLVGISVAIINLISLFGVWIGGFIADKIK
HHHHHHCCCCEEEEHHHHHCCCHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RGRKFPMLLYSIIFVALIYPILYLGLSKSFSLATSVFSIQAFLEAMIFSTLPAFLAEQFS
CCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKYRTTGVGFTYNGGAIAGGFAISAIFALSTSLGLLYAWFLNMIIAGIIMIIGIVLAKET
HHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YSGKQDPIME
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]