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The map label for this gene is hyuA [H]

Identifier: 227826919

GI number: 227826919

Start: 505528

End: 507111

Strand: Direct

Name: hyuA [H]

Synonym: M1425_0560

Alternate gene names: 227826919

Gene position: 505528-507111 (Clockwise)

Preceding gene: 227826918

Following gene: 227826921

Centisome position: 19.38

GC content: 37.18

Gene sequence:

>1584_bases
ATGATGTGGATTTCATACCACTCGAGGAAAGGTGGAAGAAGGTTGAGAATTAGAATAGGTATTGATATTGGTAGTACTCA
TACGGATGCAGTAGCATTAGAAGGTAAAGAACTAATAGTAGCGGATAAAGTAATGACTACGCCAGACTTAACCACTGGAC
TTTTAAATGCCATAAGTAAAGTAATGGAAAAACTTGGAGAGAGAAAAAACGAAGTAGATACACTGATGATAGGAACTACT
CATGGTCTAAACGCTTTACATCAAGGTAAAGGCTTAAATAGAGTAGCAATCATTAGAATTGGCTTACCGGCTGGGGAAGG
AGTTCCTCCAGTATTTGACTGGCCCGAGCAACTGGCAAGCTTTGTTACTTATAAATATATGGTAAGAGGAGGGCATGAAT
ATACTGGGGAAGAAATAGTAGAGATGGATGAGGACAAAATAAGGAAGATCGCTGAGGATATAAGCGGTAAAGTCGATGCC
ATAGCCATTAGTTCAATATTTTCAGTGGTAAATTCATCACATGAGATTAGAGCAAAGGAAATTTTAAGAGAGAAAGGAAT
TAATGTCCCCATAGTTCTTTCTCATGAAATCGGTGGAATAGGACTGCTAGAGAGGGAGAATTCAGCAATCTTAAATGCGT
TGATACTTAAAATCTTTGAGAACTTAATAAACAAAATCAAACAGTTACTATCTTCTTTGGGCATAGAAAATGTGAAACTA
TTCTTTGCACAGAATGATGGAACTGTAGCTTCTGAAGATTTTATCAAAAACTATCCAATATTCACTGTAGCAGGGCCAGT
TTCAAATAGTATTAGAGGAGCTCACTTACTGACTGGAATAAAAGATGCCATAGTGATGGACGTAGGAGGTACTACGACAA
ATGTGGGTGTTCTCCATGAGGGATATCCCAGAGAATCCTCATCTGTTGTAGAGATAGCTAAAATAAGGACTAATTTCAGA
ATGCCTGACATATACACGATGGCATTGGGAGGAGGCACGATAGTTAATAAAGAGAAAATAGGACCAGAGAGTGTAGGTTA
TACGTTAATAAATAAAGGAATAGCGTGGGGAGGCGATACTTTAACTGCAACAGATGTAGCTATGATAGTAAAAGGAATAA
CAATAGACGGTACAAATCCGCAGTTAATAATTAACAAATTCCCTCCAGAGTACTTCCTTAGTATATACACTAAAATGGTA
GGAATGTGGGAAGACGCTATAGACTTAATGAAAACTTCAAAGGATGATGTAACGGTAATTGTTGTGGGTGGTGGAAGTAT
AATGGTTCCAGAGAAATTAAAAGGGGCAATGGAAGTTATAAGACCACAAAATGCACAATACGCTAATGCGATAGGTGCAA
CATTAACCAAAGTTGGCGCTATAATGGAAAGGACGTTCTCTTATGATCAAATAACTAGAGAAAATGCTATAAAGAGTTTA
ATTGGTGAGGCTAAAAGTCTAGCCATAAAAGCTGGAGCCTTACATACAACAATAGAAGTTAGAGAAATAGAAGAAATGCA
AATTCCTTATCTACCTGGAAATTCAGTGAAAGTTAAAGTTAAGGTAGTTGGCGAATTTTCTTAA

Upstream 100 bases:

>100_bases
ATAAAATATGGTCAAAAAGTCATGGTTATCGGTATACCTTCAAGCATTAGACTGAGGAGTAAGGAAGCGTTAAGGTATAT
AGGACCCAGAGCCTTTGGAT

Downstream 100 bases:

>100_bases
TTTCTTCAAATACCATTTCCTTAATCTTATGGTATCCACCAACTTCTTTCCACTTATTACTTACAGGGATTTCTAATACT
CTCACCTTTATTCCGTACCT

Product: hydantoinase/oxoprolinase

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 527; Mature: 527

Protein sequence:

>527_residues
MMWISYHSRKGGRRLRIRIGIDIGSTHTDAVALEGKELIVADKVMTTPDLTTGLLNAISKVMEKLGERKNEVDTLMIGTT
HGLNALHQGKGLNRVAIIRIGLPAGEGVPPVFDWPEQLASFVTYKYMVRGGHEYTGEEIVEMDEDKIRKIAEDISGKVDA
IAISSIFSVVNSSHEIRAKEILREKGINVPIVLSHEIGGIGLLERENSAILNALILKIFENLINKIKQLLSSLGIENVKL
FFAQNDGTVASEDFIKNYPIFTVAGPVSNSIRGAHLLTGIKDAIVMDVGGTTTNVGVLHEGYPRESSSVVEIAKIRTNFR
MPDIYTMALGGGTIVNKEKIGPESVGYTLINKGIAWGGDTLTATDVAMIVKGITIDGTNPQLIINKFPPEYFLSIYTKMV
GMWEDAIDLMKTSKDDVTVIVVGGGSIMVPEKLKGAMEVIRPQNAQYANAIGATLTKVGAIMERTFSYDQITRENAIKSL
IGEAKSLAIKAGALHTTIEVREIEEMQIPYLPGNSVKVKVKVVGEFS

Sequences:

>Translated_527_residues
MMWISYHSRKGGRRLRIRIGIDIGSTHTDAVALEGKELIVADKVMTTPDLTTGLLNAISKVMEKLGERKNEVDTLMIGTT
HGLNALHQGKGLNRVAIIRIGLPAGEGVPPVFDWPEQLASFVTYKYMVRGGHEYTGEEIVEMDEDKIRKIAEDISGKVDA
IAISSIFSVVNSSHEIRAKEILREKGINVPIVLSHEIGGIGLLERENSAILNALILKIFENLINKIKQLLSSLGIENVKL
FFAQNDGTVASEDFIKNYPIFTVAGPVSNSIRGAHLLTGIKDAIVMDVGGTTTNVGVLHEGYPRESSSVVEIAKIRTNFR
MPDIYTMALGGGTIVNKEKIGPESVGYTLINKGIAWGGDTLTATDVAMIVKGITIDGTNPQLIINKFPPEYFLSIYTKMV
GMWEDAIDLMKTSKDDVTVIVVGGGSIMVPEKLKGAMEVIRPQNAQYANAIGATLTKVGAIMERTFSYDQITRENAIKSL
IGEAKSLAIKAGALHTTIEVREIEEMQIPYLPGNSVKVKVKVVGEFS
>Mature_527_residues
MMWISYHSRKGGRRLRIRIGIDIGSTHTDAVALEGKELIVADKVMTTPDLTTGLLNAISKVMEKLGERKNEVDTLMIGTT
HGLNALHQGKGLNRVAIIRIGLPAGEGVPPVFDWPEQLASFVTYKYMVRGGHEYTGEEIVEMDEDKIRKIAEDISGKVDA
IAISSIFSVVNSSHEIRAKEILREKGINVPIVLSHEIGGIGLLERENSAILNALILKIFENLINKIKQLLSSLGIENVKL
FFAQNDGTVASEDFIKNYPIFTVAGPVSNSIRGAHLLTGIKDAIVMDVGGTTTNVGVLHEGYPRESSSVVEIAKIRTNFR
MPDIYTMALGGGTIVNKEKIGPESVGYTLINKGIAWGGDTLTATDVAMIVKGITIDGTNPQLIINKFPPEYFLSIYTKMV
GMWEDAIDLMKTSKDDVTVIVVGGGSIMVPEKLKGAMEVIRPQNAQYANAIGATLTKVGAIMERTFSYDQITRENAIKSL
IGEAKSLAIKAGALHTTIEVREIEEMQIPYLPGNSVKVKVKVVGEFS

Specific function: HyuA and hyuB convert D- and L-substituted hydantoins to corresponding N-carbamyl-amino acids [H]

COG id: COG0145

COG function: function code EQ; N-methylhydantoinase A/acetone carboxylase, beta subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the oxoprolinase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008040
- InterPro:   IPR002821 [H]

Pfam domain/function: PF05378 Hydant_A_N; PF01968 Hydantoinase_A [H]

EC number: NA

Molecular weight: Translated: 57343; Mature: 57343

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMWISYHSRKGGRRLRIRIGIDIGSTHTDAVALEGKELIVADKVMTTPDLTTGLLNAISK
CEEEEEECCCCCCEEEEEEEEEECCCCCCEEEECCCEEEEECCEECCCCHHHHHHHHHHH
VMEKLGERKNEVDTLMIGTTHGLNALHQGKGLNRVAIIRIGLPAGEGVPPVFDWPEQLAS
HHHHHCCCCCCCCEEEEECCCCCCHHHCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHH
FVTYKYMVRGGHEYTGEEIVEMDEDKIRKIAEDISGKVDAIAISSIFSVVNSSHEIRAKE
HHHHHHHHCCCCCCCCHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHH
ILREKGINVPIVLSHEIGGIGLLERENSAILNALILKIFENLINKIKQLLSSLGIENVKL
HHHHCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
FFAQNDGTVASEDFIKNYPIFTVAGPVSNSIRGAHLLTGIKDAIVMDVGGTTTNVGVLHE
EEEECCCCCCCHHHHHCCCEEEEECCCCCCCCHHHHHHCCCCEEEEECCCCCCEEEEEEC
GYPRESSSVVEIAKIRTNFRMPDIYTMALGGGTIVNKEKIGPESVGYTLINKGIAWGGDT
CCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCEEEECCCCCCHHCCHHHHHCCCCCCCCC
LTATDVAMIVKGITIDGTNPQLIINKFPPEYFLSIYTKMVGMWEDAIDLMKTSKDDVTVI
CHHHHHHHHHCCEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
VVGGGSIMVPEKLKGAMEVIRPQNAQYANAIGATLTKVGAIMERTFSYDQITRENAIKSL
EECCCCEECCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
IGEAKSLAIKAGALHTTIEVREIEEMQIPYLPGNSVKVKVKVVGEFS
HHHHHHHEEECCCEEEEEEEEEHHHCCCCCCCCCCEEEEEEEEECCC
>Mature Secondary Structure
MMWISYHSRKGGRRLRIRIGIDIGSTHTDAVALEGKELIVADKVMTTPDLTTGLLNAISK
CEEEEEECCCCCCEEEEEEEEEECCCCCCEEEECCCEEEEECCEECCCCHHHHHHHHHHH
VMEKLGERKNEVDTLMIGTTHGLNALHQGKGLNRVAIIRIGLPAGEGVPPVFDWPEQLAS
HHHHHCCCCCCCCEEEEECCCCCCHHHCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHH
FVTYKYMVRGGHEYTGEEIVEMDEDKIRKIAEDISGKVDAIAISSIFSVVNSSHEIRAKE
HHHHHHHHCCCCCCCCHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHH
ILREKGINVPIVLSHEIGGIGLLERENSAILNALILKIFENLINKIKQLLSSLGIENVKL
HHHHCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
FFAQNDGTVASEDFIKNYPIFTVAGPVSNSIRGAHLLTGIKDAIVMDVGGTTTNVGVLHE
EEEECCCCCCCHHHHHCCCEEEEECCCCCCCCHHHHHHCCCCEEEEECCCCCCEEEEEEC
GYPRESSSVVEIAKIRTNFRMPDIYTMALGGGTIVNKEKIGPESVGYTLINKGIAWGGDT
CCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCEEEECCCCCCHHCCHHHHHCCCCCCCCC
LTATDVAMIVKGITIDGTNPQLIINKFPPEYFLSIYTKMVGMWEDAIDLMKTSKDDVTVI
CHHHHHHHHHCCEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
VVGGGSIMVPEKLKGAMEVIRPQNAQYANAIGATLTKVGAIMERTFSYDQITRENAIKSL
EECCCCEECCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
IGEAKSLAIKAGALHTTIEVREIEEMQIPYLPGNSVKVKVKVVGEFS
HHHHHHHEEECCCEEEEEEEEEHHHCCCCCCCCCCEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1732229 [H]