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The map label for this gene is hycF [C]

Identifier: 227826901

GI number: 227826901

Start: 478812

End: 480179

Strand: Direct

Name: hycF [C]

Synonym: M1425_0534

Alternate gene names: 227826901

Gene position: 478812-480179 (Clockwise)

Preceding gene: 227826900

Following gene: 227826902

Centisome position: 18.35

GC content: 35.67

Gene sequence:

>1368_bases
ATGACATCCCTTTTTTATTCACCCCTTATTAAATACCGAGTAGATGTTTTGCCCTCATCTGAGTTGAAAAAGGAAAACAT
TAATACCAAAGCCTTAGTCGTTATAGGAGATGGAATTGATAGGGAAAAAATTTCAGAAGATCTAGAGTTAAACCCCTTGC
TGGTAAGAATAGTTGACAAGGATAGTAGTAAGGAAGAGGTAGAATACAACAAAGTAGTTCTAGAAAACGCCTGGCTAGCA
GATTTAGCTCCAGTTGAAGAGATAAAATCCATTGATAGACGAAGTCTTTTACGCGGAGAAGTAAAAAAGGCTAAAAAGGT
TGACAAGCCAATTTACCTTAGTGAATATTGTAATGGCTTATATAAGGCATGTAATGTATGTGAGTTTTCATGTCCTTACA
ACGCTATTAAAGTTGATAAGAAAACTGGAGTAAATATTGATTACATAAAATGTACATCTTGTGGTCTATGCGTAGCTTCA
TGCCCAGTTAGTGCTATACAATTTCCATCACTTTCTCAAAATTCGATTTTTGAATTAGCAAAGGTAAAAGGGGAAAAGAG
GATTACATGCTATAAAAATACTAAAAATAGAGGAGTCAAGATACCTTGTTTAGCAATGCTATCGGAGGTAGATATAGTAT
TATTGAAAAGCAGTGGGAATTTAACCTTTGAATGTCCAGGATGTGAATTTCAAGATAATCTAAAGCATTTCATTGAAGTG
ATCAAGGAATATAACGAGAGAATAGGAGGAATCAGCTTTTATTCCCCATCTGAAAAAATTGAGGCAAAAGAGACAAAGGA
ACTAAATACTACTCCACAGTCATTTTACAATAGGGCTGAGGCTAGAAGGAACATAAGTGATGAATTGCCATACATATTAT
TTGATGTGAGTATTGATAATAACAGATGTACTTTATGTGAAAGCTGCGTTAATTGGTGCCCCACATCAGCACTAAAGCTC
AGAAGGGATAGTGGAGTGGAAGAAATAGACTTTGATCCCAAAAGATGTATAGGCTGTAATTTGTGTGTCAATGTTTGCCC
AGAATCGTGCAAATTAGAAGAAGGTAAAACAAGTGAGATTCCTCCAAATATAGCATCTCTTACTAAGGTTATTAAGGTGG
AAAAGAGTAAAAGCGTTAACAAAGAGGTAAGGAAATTAGTGGGCGATGAATTAGTCAGATGTAGAGTTTGTGGAGCACCA
ATAGGTTCGAGGAAAAGTCTTAATCATGTTAAGAAAATAATGATTGAAAAGGGGGCTTCGTGTGAAGATGAGTGGTTGGA
GAGATGTCCTAAGCATAGGGTAGAGTACGCGTTTCAGAAACAATTTTCATTTAATGCAAGATTTAAACCTAGAGGTGATC
TAAGATGA

Upstream 100 bases:

>100_bases
CCCAGTACTACTATTACTTGGGATTTACACATTGGGCGTAATACTATTACCATTAGAAGAAGATGAAAAGCCAAGACACC
TTATATTCAAGTGAAGTCAT

Downstream 100 bases:

>100_bases
AGTCTCTCTCTACGGATTTCAAGATCTTTTCCTTTTTATTTCTGAGCCCTAGATATATTAAAGACGTTAAAGGGTTTGTG
GAGGAGATAAAGGATAAGCC

Product: 4Fe-4S ferredoxin iron-sulfur binding domain protein

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MTSLFYSPLIKYRVDVLPSSELKKENINTKALVVIGDGIDREKISEDLELNPLLVRIVDKDSSKEEVEYNKVVLENAWLA
DLAPVEEIKSIDRRSLLRGEVKKAKKVDKPIYLSEYCNGLYKACNVCEFSCPYNAIKVDKKTGVNIDYIKCTSCGLCVAS
CPVSAIQFPSLSQNSIFELAKVKGEKRITCYKNTKNRGVKIPCLAMLSEVDIVLLKSSGNLTFECPGCEFQDNLKHFIEV
IKEYNERIGGISFYSPSEKIEAKETKELNTTPQSFYNRAEARRNISDELPYILFDVSIDNNRCTLCESCVNWCPTSALKL
RRDSGVEEIDFDPKRCIGCNLCVNVCPESCKLEEGKTSEIPPNIASLTKVIKVEKSKSVNKEVRKLVGDELVRCRVCGAP
IGSRKSLNHVKKIMIEKGASCEDEWLERCPKHRVEYAFQKQFSFNARFKPRGDLR

Sequences:

>Translated_455_residues
MTSLFYSPLIKYRVDVLPSSELKKENINTKALVVIGDGIDREKISEDLELNPLLVRIVDKDSSKEEVEYNKVVLENAWLA
DLAPVEEIKSIDRRSLLRGEVKKAKKVDKPIYLSEYCNGLYKACNVCEFSCPYNAIKVDKKTGVNIDYIKCTSCGLCVAS
CPVSAIQFPSLSQNSIFELAKVKGEKRITCYKNTKNRGVKIPCLAMLSEVDIVLLKSSGNLTFECPGCEFQDNLKHFIEV
IKEYNERIGGISFYSPSEKIEAKETKELNTTPQSFYNRAEARRNISDELPYILFDVSIDNNRCTLCESCVNWCPTSALKL
RRDSGVEEIDFDPKRCIGCNLCVNVCPESCKLEEGKTSEIPPNIASLTKVIKVEKSKSVNKEVRKLVGDELVRCRVCGAP
IGSRKSLNHVKKIMIEKGASCEDEWLERCPKHRVEYAFQKQFSFNARFKPRGDLR
>Mature_454_residues
TSLFYSPLIKYRVDVLPSSELKKENINTKALVVIGDGIDREKISEDLELNPLLVRIVDKDSSKEEVEYNKVVLENAWLAD
LAPVEEIKSIDRRSLLRGEVKKAKKVDKPIYLSEYCNGLYKACNVCEFSCPYNAIKVDKKTGVNIDYIKCTSCGLCVASC
PVSAIQFPSLSQNSIFELAKVKGEKRITCYKNTKNRGVKIPCLAMLSEVDIVLLKSSGNLTFECPGCEFQDNLKHFIEVI
KEYNERIGGISFYSPSEKIEAKETKELNTTPQSFYNRAEARRNISDELPYILFDVSIDNNRCTLCESCVNWCPTSALKLR
RDSGVEEIDFDPKRCIGCNLCVNVCPESCKLEEGKTSEIPPNIASLTKVIKVEKSKSVNKEVRKLVGDELVRCRVCGAPI
GSRKSLNHVKKIMIEKGASCEDEWLERCPKHRVEYAFQKQFSFNARFKPRGDLR

Specific function: Probable Electron Transfer Protein For Hydrogenase 3 [C]

COG id: COG1145

COG function: function code C; Ferredoxin

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 51486; Mature: 51355

Theoretical pI: Translated: 8.14; Mature: 8.14

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.5 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
5.5 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSLFYSPLIKYRVDVLPSSELKKENINTKALVVIGDGIDREKISEDLELNPLLVRIVDK
CCCHHHHHHHHHHHHCCCCHHHHHCCCCCEEEEEECCCCCHHHHHCCCCCCCEEEEEECC
DSSKEEVEYNKVVLENAWLADLAPVEEIKSIDRRSLLRGEVKKAKKVDKPIYLSEYCNGL
CCCHHHHHHHHEEECCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHH
YKACNVCEFSCPYNAIKVDKKTGVNIDYIKCTSCGLCVASCPVSAIQFPSLSQNSIFELA
HHHCCCEECCCCCCEEEECCCCCCEEEEEEECCCCCCHHHCCCHHHCCCCCCCHHHHHHH
KVKGEKRITCYKNTKNRGVKIPCLAMLSEVDIVLLKSSGNLTFECPGCEFQDNLKHFIEV
HHCCCCEEEEEECCCCCCEECHHHHHHCCCCEEEEECCCCEEEECCCCCHHHHHHHHHHH
IKEYNERIGGISFYSPSEKIEAKETKELNTTPQSFYNRAEARRNISDELPYILFDVSIDN
HHHHHHHHCCEEEECCCHHCCHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEEEEEEEECC
NRCTLCESCVNWCPTSALKLRRDSGVEEIDFDPKRCIGCNLCVNVCPESCKLEEGKTSEI
CCCHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHCCCHHHHHCCCCCCCCCCCCCCCC
PPNIASLTKVIKVEKSKSVNKEVRKLVGDELVRCRVCGAPIGSRKSLNHVKKIMIEKGAS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHCCCC
CEDEWLERCPKHRVEYAFQKQFSFNARFKPRGDLR
CCHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TSLFYSPLIKYRVDVLPSSELKKENINTKALVVIGDGIDREKISEDLELNPLLVRIVDK
CCHHHHHHHHHHHHCCCCHHHHHCCCCCEEEEEECCCCCHHHHHCCCCCCCEEEEEECC
DSSKEEVEYNKVVLENAWLADLAPVEEIKSIDRRSLLRGEVKKAKKVDKPIYLSEYCNGL
CCCHHHHHHHHEEECCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHH
YKACNVCEFSCPYNAIKVDKKTGVNIDYIKCTSCGLCVASCPVSAIQFPSLSQNSIFELA
HHHCCCEECCCCCCEEEECCCCCCEEEEEEECCCCCCHHHCCCHHHCCCCCCCHHHHHHH
KVKGEKRITCYKNTKNRGVKIPCLAMLSEVDIVLLKSSGNLTFECPGCEFQDNLKHFIEV
HHCCCCEEEEEECCCCCCEECHHHHHHCCCCEEEEECCCCEEEECCCCCHHHHHHHHHHH
IKEYNERIGGISFYSPSEKIEAKETKELNTTPQSFYNRAEARRNISDELPYILFDVSIDN
HHHHHHHHCCEEEECCCHHCCHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEEEEEEEECC
NRCTLCESCVNWCPTSALKLRRDSGVEEIDFDPKRCIGCNLCVNVCPESCKLEEGKTSEI
CCCHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHCCCHHHHHCCCCCCCCCCCCCCCC
PPNIASLTKVIKVEKSKSVNKEVRKLVGDELVRCRVCGAPIGSRKSLNHVKKIMIEKGAS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHCCCC
CEDEWLERCPKHRVEYAFQKQFSFNARFKPRGDLR
CCHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA