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The map label for this gene is nrfC [H]

Identifier: 227826899

GI number: 227826899

Start: 476827

End: 477513

Strand: Direct

Name: nrfC [H]

Synonym: M1425_0532

Alternate gene names: 227826899

Gene position: 476827-477513 (Clockwise)

Preceding gene: 227826898

Following gene: 227826900

Centisome position: 18.28

GC content: 42.21

Gene sequence:

>687_bases
ATGTCCACACAAGTTTCAAACCCATATATGCAATTTGGGAATACTCAACCTTGTAAACACTGGGGATTCGTGGTTAAGGT
AGACCAATGTCTAGGCTGTGATGCTTGTGTTGCTGCGTGCACAATAGAGAATCAAACGCCATTCTGGCGAGGTCTATTTA
GAACTCATGTTGAAGATCTAGAATTAGGAGAATATCCCAACACTCAGAGAGTCTTCGTACCTAGATTGTGTATGCAATGT
GAAAACCCGCCATGTTATTATGTATGTCCAACTGGAGCTACCCAAATTGTTGCGGGAGGTATTGTAGTGGTTGACGAGTA
TAAATGTATGGGATGTCTTTACTGTGTTGAGGCATGTCCATACGGTGCTAGATACTTCTACACTTACGAGGATATTGAGA
AGTCCAAGGAGTATTTTGGATTGAATTCAATTCACGTGGTTCCTCACGTAGATAAATGTACTTTCTGCTACGGGACAGCC
CCAGAAGGCACTTATACTCCTGCATGTGTAAGAACTTGTCCGGGTCATGCCAGAGTATTTGGGTGTCTTGATGATCCTAA
TAGTGAGGTTAGTATATTAGTTAATACTGGGCAAGCAGTAGTGTTGAATCCGGAATTAAACGTTCAACCTAAAGTTTTTT
ACGTGTTTAATAGGCAGTTGGGTAGGGGAGGAGGTGGCAACTCATGA

Upstream 100 bases:

>100_bases
ACAGCAAGCTAAAGTTACCACTCCTAACAATTCCGCTTCTCAAAATGATTGGGAACAATACATCTCATACGATAATGTTT
CCACATTAGGGTGATCCCTC

Downstream 100 bases:

>100_bases
ATAAATTCCTACTAGCTTGGACTATAATATTCATAATAATTGGAGGAGGGATATTACTTTATGGTATGAGCTTTAATCCC
ATGTCATGGGCTAATGGCCT

Product: 4Fe-4S ferredoxin iron-sulfur binding domain protein

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: NA

Number of amino acids: Translated: 228; Mature: 227

Protein sequence:

>228_residues
MSTQVSNPYMQFGNTQPCKHWGFVVKVDQCLGCDACVAACTIENQTPFWRGLFRTHVEDLELGEYPNTQRVFVPRLCMQC
ENPPCYYVCPTGATQIVAGGIVVVDEYKCMGCLYCVEACPYGARYFYTYEDIEKSKEYFGLNSIHVVPHVDKCTFCYGTA
PEGTYTPACVRTCPGHARVFGCLDDPNSEVSILVNTGQAVVLNPELNVQPKVFYVFNRQLGRGGGGNS

Sequences:

>Translated_228_residues
MSTQVSNPYMQFGNTQPCKHWGFVVKVDQCLGCDACVAACTIENQTPFWRGLFRTHVEDLELGEYPNTQRVFVPRLCMQC
ENPPCYYVCPTGATQIVAGGIVVVDEYKCMGCLYCVEACPYGARYFYTYEDIEKSKEYFGLNSIHVVPHVDKCTFCYGTA
PEGTYTPACVRTCPGHARVFGCLDDPNSEVSILVNTGQAVVLNPELNVQPKVFYVFNRQLGRGGGGNS
>Mature_227_residues
STQVSNPYMQFGNTQPCKHWGFVVKVDQCLGCDACVAACTIENQTPFWRGLFRTHVEDLELGEYPNTQRVFVPRLCMQCE
NPPCYYVCPTGATQIVAGGIVVVDEYKCMGCLYCVEACPYGARYFYTYEDIEKSKEYFGLNSIHVVPHVDKCTFCYGTAP
EGTYTPACVRTCPGHARVFGCLDDPNSEVSILVNTGQAVVLNPELNVQPKVFYVFNRQLGRGGGGNS

Specific function: Probably involved in the transfer of electrons from the quinone pool to the type-c cytochromes [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI2367345, Length=198, Percent_Identity=36.3636363636364, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI226510944, Length=176, Percent_Identity=33.5227272727273, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1787122, Length=206, Percent_Identity=32.5242718446602, Blast_Score=84, Evalue=5e-18,
Organism=Escherichia coli, GI1787872, Length=206, Percent_Identity=31.0679611650485, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1787740, Length=141, Percent_Identity=33.3333333333333, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI1789067, Length=175, Percent_Identity=30.8571428571429, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787478, Length=129, Percent_Identity=33.3333333333333, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI1787749, Length=216, Percent_Identity=27.7777777777778, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR017567
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 25316; Mature: 25185

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

7.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
9.6 %Cys+Met (Translated Protein)
7.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
9.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTQVSNPYMQFGNTQPCKHWGFVVKVDQCLGCDACVAACTIENQTPFWRGLFRTHVEDL
CCCCCCCCHHHCCCCCCCCCCCEEEEEHHHCCCCHHEEEEEECCCCCHHHHHHHHHHHHC
ELGEYPNTQRVFVPRLCMQCENPPCYYVCPTGATQIVAGGIVVVDEYKCMGCLYCVEACP
CCCCCCCCCEEEHHHHHHHCCCCCEEEECCCCCHHEEECCEEEEECCCHHHHHHHHHHCC
YGARYFYTYEDIEKSKEYFGLNSIHVVPHVDKCTFCYGTAPEGTYTPACVRTCPGHARVF
CCCEEEEEHHHHHHHHHHCCCCEEEEEECCCCEEEEECCCCCCCCCCHHHHCCCCCEEEE
GCLDDPNSEVSILVNTGQAVVLNPELNVQPKVFYVFNRQLGRGGGGNS
EECCCCCCCEEEEEECCCEEEECCCCCCCCEEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
STQVSNPYMQFGNTQPCKHWGFVVKVDQCLGCDACVAACTIENQTPFWRGLFRTHVEDL
CCCCCCCHHHCCCCCCCCCCCEEEEEHHHCCCCHHEEEEEECCCCCHHHHHHHHHHHHC
ELGEYPNTQRVFVPRLCMQCENPPCYYVCPTGATQIVAGGIVVVDEYKCMGCLYCVEACP
CCCCCCCCCEEEHHHHHHHCCCCCEEEECCCCCHHEEECCEEEEECCCHHHHHHHHHHCC
YGARYFYTYEDIEKSKEYFGLNSIHVVPHVDKCTFCYGTAPEGTYTPACVRTCPGHARVF
CCCEEEEEHHHHHHHHHHCCCCEEEEEECCCCEEEEECCCCCCCCCCHHHHCCCCCEEEE
GCLDDPNSEVSILVNTGQAVVLNPELNVQPKVFYVFNRQLGRGGGGNS
EECCCCCCCEEEEEECCCEEEECCCCCCCCEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]