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The map label for this gene is pbpF [H]

Identifier: 227815699

GI number: 227815699

Start: 2844368

End: 2846359

Strand: Reverse

Name: pbpF [H]

Synonym: BAMEG_3119

Alternate gene names: 227815699

Gene position: 2846359-2844368 (Counterclockwise)

Preceding gene: 227815703

Following gene: 227815695

Centisome position: 54.42

GC content: 35.64

Gene sequence:

>1992_bases
ATGCAGGAGTGGTTCAACAAGAGAAAGAAGCTTCGTAATTTATTAATTATATTAGGAAGTTTACTCGCTACTCTATTTAT
TGCTATAAATATAATAATTTCCATTCAAGACATATCTGAATTAAAGCAAGCTGTTCCGCAACCGACTTTAATTTATGATG
CAAATAATGAAGTTGCGACTAAATTAGCTTCTTCTAAAACAGAAGGTGTGAAAAGAAAAGATATTCCTGATATTATGGTT
CAAGCAATTGTTGCAGTAGAAGATAAGGAATTTTTTAACCATCACGGTATTTATTATAGTGGAATTATAAGTGCGATTTT
TAAAAATATTACAGCAGGAGAAGTTGTAGCAGGGGGAAGTACAATTACACAACAACTTGCGAAAAATGTATTTCTGACAC
AAGACCGCACATACTCTCGAAAAATAAAGGAATATTTTTTAACAAAAAAAATAGAGCGTACGTATACGAAAGATGAAATC
ATTGAAATGTATATGAACCAAATTTATTTTGGTGAAGGTGCTTGGGGTATAAAAAGGGCAGCTAAATCTTATTTTGATAA
AGATGTAAAAGATTTAACAATTTCAGAAGCTGCAACGATTGCAGGCTTGATTAAAGCACCATCTGCGTATTCACCTTATA
AAAACTTTAACAAGTCAATTGAAAGACGTAATGTCGTATTAAGTTTAATGAAAGAGCAAGGGTATATTTCTGACGAACAG
TATAATAAAGAAAAAGAGAGCGGGCTTGTGTTAAAACGTGGCGTTGATGATAAATACAAGGGAAAATACTCTCAATATGT
AGACTATATTGTTAGAGAAGCGATGGACAAGTACGAATTAACACAAAATGAAATTTTAGCAGGTGGTTATCGTATTTATA
CAGAGCTTGATCCGAAAAAACAACAGGCTGTAGAAGACGTTGTGAATAATGATAGTTATTTCAAAGATAGTGGCTCAGAC
CAACTTATGCAAACGGGTGTAGTTCTTATGAATCCAACAACAGGTGGTGTACCAGCTCTAGTAGGAGGCAGAGGGCCTTA
TCAGTTTTTACAGTTTAACCATGCAACACAATTAAAAAGACAACCTGGTTCAACGCTAAAGCCTTTGGCTGTATATGTAC
CAGCGTTAGAACAAGGATATGAAGTATACGATATATTAAAAGATGAACCATTTAACATTAAAGAATATAAACCACAAAAT
AGCGATCATACTTTTCACGGTGATGTGACAATGTATGAAGCTGTGGCAAAATCGTATAATGTTTCGGCTGTGTGGCTATT
AGAACAAATTGGATTAGATAAAGGATTGAAATCTTTAGAGCGATTTGGTATTCCATTAGTACCAGAAGATCGTACGTATC
CGATTGCTTTAGGCGGTATGCATGTGGGGACATCTCCATTTGTAATGGCTCAAGCATACAGTACATTTGCAAATGACGGT
GTCCAAGTGGAAGCTCATGCAATTAGAGAGATACAAAATGCTGAAGGTGAAACGATTGGAAAGTGGTATAAGAAAGAGAC
ACGAGTGACGAGCGAGAAAATTTCTCAAAAGATGACATATTTATTAAAGGGTGTTGTCGAAAAGGGAACAGGCGAAAAGG
CGAAAGTTACTAATGTTGATACAGCAGGTAAGACAGGAACTACTCAAATTGTAAATGGCCCGAGCACCGGTGCAAAGGAT
TCATGGTTTGTTGGATACACACCAGATTTGGTAGGTGCGATTTGGGTAGGATATGATAAAACAGATAGCGATCATTATGT
TCCAGGTGGTAGTCAAATTACGACGACAATGTTCCGGGATATTATGAAAAAAGCGAACGCAAATCCAGCTCAAAAAGCAT
TCCAGTTATCACTCATATCTGAGGCTGATTATAAAAAACAATTGACTACAATAGAAGAGGAAAAACGAAGAAAAGAAGAA
GAACAACAACGAAAACAAGAGCAGCAAGAGTGGCTTGATAAAGTGAAAGAATGGATTCCTTCATTTTGGTAA

Upstream 100 bases:

>100_bases
TAATAAATTTAAAAGGAGGTATAATCTTCCTTTTAAAAAGAAGAGTGTAAACAAACAAAGGAAGTGAACGAATGAAATTG
AAGAACACTCACTTAAAAAA

Downstream 100 bases:

>100_bases
AAGAAAGAGGCGAATAAAGAAATTCGCCTCTTTTTTGTATGAAAACAAAATGAAATTTTAATTGGTAGAGATTTTTGTCT
ATCTTAATTAGCTATTAATT

Product: penicillin-binding protein

Products: [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n+1-; $diphosphoundecaprenol; undecaprenyl diphosphate

Alternate protein names: PBP-1F; Penicillin-binding protein F [H]

Number of amino acids: Translated: 663; Mature: 663

Protein sequence:

>663_residues
MQEWFNKRKKLRNLLIILGSLLATLFIAINIIISIQDISELKQAVPQPTLIYDANNEVATKLASSKTEGVKRKDIPDIMV
QAIVAVEDKEFFNHHGIYYSGIISAIFKNITAGEVVAGGSTITQQLAKNVFLTQDRTYSRKIKEYFLTKKIERTYTKDEI
IEMYMNQIYFGEGAWGIKRAAKSYFDKDVKDLTISEAATIAGLIKAPSAYSPYKNFNKSIERRNVVLSLMKEQGYISDEQ
YNKEKESGLVLKRGVDDKYKGKYSQYVDYIVREAMDKYELTQNEILAGGYRIYTELDPKKQQAVEDVVNNDSYFKDSGSD
QLMQTGVVLMNPTTGGVPALVGGRGPYQFLQFNHATQLKRQPGSTLKPLAVYVPALEQGYEVYDILKDEPFNIKEYKPQN
SDHTFHGDVTMYEAVAKSYNVSAVWLLEQIGLDKGLKSLERFGIPLVPEDRTYPIALGGMHVGTSPFVMAQAYSTFANDG
VQVEAHAIREIQNAEGETIGKWYKKETRVTSEKISQKMTYLLKGVVEKGTGEKAKVTNVDTAGKTGTTQIVNGPSTGAKD
SWFVGYTPDLVGAIWVGYDKTDSDHYVPGGSQITTTMFRDIMKKANANPAQKAFQLSLISEADYKKQLTTIEEEKRRKEE
EQQRKQEQQEWLDKVKEWIPSFW

Sequences:

>Translated_663_residues
MQEWFNKRKKLRNLLIILGSLLATLFIAINIIISIQDISELKQAVPQPTLIYDANNEVATKLASSKTEGVKRKDIPDIMV
QAIVAVEDKEFFNHHGIYYSGIISAIFKNITAGEVVAGGSTITQQLAKNVFLTQDRTYSRKIKEYFLTKKIERTYTKDEI
IEMYMNQIYFGEGAWGIKRAAKSYFDKDVKDLTISEAATIAGLIKAPSAYSPYKNFNKSIERRNVVLSLMKEQGYISDEQ
YNKEKESGLVLKRGVDDKYKGKYSQYVDYIVREAMDKYELTQNEILAGGYRIYTELDPKKQQAVEDVVNNDSYFKDSGSD
QLMQTGVVLMNPTTGGVPALVGGRGPYQFLQFNHATQLKRQPGSTLKPLAVYVPALEQGYEVYDILKDEPFNIKEYKPQN
SDHTFHGDVTMYEAVAKSYNVSAVWLLEQIGLDKGLKSLERFGIPLVPEDRTYPIALGGMHVGTSPFVMAQAYSTFANDG
VQVEAHAIREIQNAEGETIGKWYKKETRVTSEKISQKMTYLLKGVVEKGTGEKAKVTNVDTAGKTGTTQIVNGPSTGAKD
SWFVGYTPDLVGAIWVGYDKTDSDHYVPGGSQITTTMFRDIMKKANANPAQKAFQLSLISEADYKKQLTTIEEEKRRKEE
EQQRKQEQQEWLDKVKEWIPSFW
>Mature_663_residues
MQEWFNKRKKLRNLLIILGSLLATLFIAINIIISIQDISELKQAVPQPTLIYDANNEVATKLASSKTEGVKRKDIPDIMV
QAIVAVEDKEFFNHHGIYYSGIISAIFKNITAGEVVAGGSTITQQLAKNVFLTQDRTYSRKIKEYFLTKKIERTYTKDEI
IEMYMNQIYFGEGAWGIKRAAKSYFDKDVKDLTISEAATIAGLIKAPSAYSPYKNFNKSIERRNVVLSLMKEQGYISDEQ
YNKEKESGLVLKRGVDDKYKGKYSQYVDYIVREAMDKYELTQNEILAGGYRIYTELDPKKQQAVEDVVNNDSYFKDSGSD
QLMQTGVVLMNPTTGGVPALVGGRGPYQFLQFNHATQLKRQPGSTLKPLAVYVPALEQGYEVYDILKDEPFNIKEYKPQN
SDHTFHGDVTMYEAVAKSYNVSAVWLLEQIGLDKGLKSLERFGIPLVPEDRTYPIALGGMHVGTSPFVMAQAYSTFANDG
VQVEAHAIREIQNAEGETIGKWYKKETRVTSEKISQKMTYLLKGVVEKGTGEKAKVTNVDTAGKTGTTQIVNGPSTGAKD
SWFVGYTPDLVGAIWVGYDKTDSDHYVPGGSQITTTMFRDIMKKANANPAQKAFQLSLISEADYKKQLTTIEEEKRRKEE
EQQRKQEQQEWLDKVKEWIPSFW

Specific function: Cell wall formation. May be involved in outgrowth of the germinated spore or it could function in the synthesis of the germ cell wall [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=531, Percent_Identity=32.015065913371, Blast_Score=239, Evalue=5e-64,
Organism=Escherichia coli, GI87082258, Length=283, Percent_Identity=39.5759717314488, Blast_Score=197, Evalue=2e-51,
Organism=Escherichia coli, GI1788867, Length=514, Percent_Identity=27.6264591439689, Blast_Score=160, Evalue=3e-40,
Organism=Escherichia coli, GI1789601, Length=143, Percent_Identity=37.0629370629371, Blast_Score=93, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129

Molecular weight: Translated: 74909; Mature: 74909

Theoretical pI: Translated: 8.31; Mature: 8.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEWFNKRKKLRNLLIILGSLLATLFIAINIIISIQDISELKQAVPQPTLIYDANNEVAT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHH
KLASSKTEGVKRKDIPDIMVQAIVAVEDKEFFNHHGIYYSGIISAIFKNITAGEVVAGGS
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEECCH
TITQQLAKNVFLTQDRTYSRKIKEYFLTKKIERTYTKDEIIEMYMNQIYFGEGAWGIKRA
HHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHEECCCCHHHHHH
AKSYFDKDVKDLTISEAATIAGLIKAPSAYSPYKNFNKSIERRNVVLSLMKEQGYISDEQ
HHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHH
YNKEKESGLVLKRGVDDKYKGKYSQYVDYIVREAMDKYELTQNEILAGGYRIYTELDPKK
HCCHHHCCEEEECCCCHHHCCCHHHHHHHHHHHHHHHHHCCHHHEEECCEEEEEECCCHH
QQAVEDVVNNDSYFKDSGSDQLMQTGVVLMNPTTGGVPALVGGRGPYQFLQFNHATQLKR
HHHHHHHHCCCCCCCCCCCHHHHHCCEEEECCCCCCCCEEECCCCCHHHHHCCCHHHHHC
QPGSTLKPLAVYVPALEQGYEVYDILKDEPFNIKEYKPQNSDHTFHGDVTMYEAVAKSYN
CCCCCCCCHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHCCC
VSAVWLLEQIGLDKGLKSLERFGIPLVPEDRTYPIALGGMHVGTSPFVMAQAYSTFANDG
CHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHCCCC
VQVEAHAIREIQNAEGETIGKWYKKETRVTSEKISQKMTYLLKGVVEKGTGEKAKVTNVD
EEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCC
TAGKTGTTQIVNGPSTGAKDSWFVGYTPDLVGAIWVGYDKTDSDHYVPGGSQITTTMFRD
CCCCCCCCEEECCCCCCCCCCEEECCCHHHHHHEEECCCCCCCCCCCCCCCHHHHHHHHH
IMKKANANPAQKAFQLSLISEADYKKQLTTIEEEKRRKEEEQQRKQEQQEWLDKVKEWIP
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SFW
CCC
>Mature Secondary Structure
MQEWFNKRKKLRNLLIILGSLLATLFIAINIIISIQDISELKQAVPQPTLIYDANNEVAT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHH
KLASSKTEGVKRKDIPDIMVQAIVAVEDKEFFNHHGIYYSGIISAIFKNITAGEVVAGGS
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEECCH
TITQQLAKNVFLTQDRTYSRKIKEYFLTKKIERTYTKDEIIEMYMNQIYFGEGAWGIKRA
HHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHEECCCCHHHHHH
AKSYFDKDVKDLTISEAATIAGLIKAPSAYSPYKNFNKSIERRNVVLSLMKEQGYISDEQ
HHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHH
YNKEKESGLVLKRGVDDKYKGKYSQYVDYIVREAMDKYELTQNEILAGGYRIYTELDPKK
HCCHHHCCEEEECCCCHHHCCCHHHHHHHHHHHHHHHHHCCHHHEEECCEEEEEECCCHH
QQAVEDVVNNDSYFKDSGSDQLMQTGVVLMNPTTGGVPALVGGRGPYQFLQFNHATQLKR
HHHHHHHHCCCCCCCCCCCHHHHHCCEEEECCCCCCCCEEECCCCCHHHHHCCCHHHHHC
QPGSTLKPLAVYVPALEQGYEVYDILKDEPFNIKEYKPQNSDHTFHGDVTMYEAVAKSYN
CCCCCCCCHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHCCC
VSAVWLLEQIGLDKGLKSLERFGIPLVPEDRTYPIALGGMHVGTSPFVMAQAYSTFANDG
CHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHCCCC
VQVEAHAIREIQNAEGETIGKWYKKETRVTSEKISQKMTYLLKGVVEKGTGEKAKVTNVD
EEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCC
TAGKTGTTQIVNGPSTGAKDSWFVGYTPDLVGAIWVGYDKTDSDHYVPGGSQITTTMFRD
CCCCCCCCEEECCCCCCCCCCEEECCCHHHHHHEEECCCCCCCCCCCCCCCHHHHHHHHH
IMKKANANPAQKAFQLSLISEADYKKQLTTIEEEKRRKEEEQQRKQEQQEWLDKVKEWIP
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SFW
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n-; $diphosphoundecaprenol; GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-; $diphosphoundecaprenol

Specific reaction: [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n- $diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu- $L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = [GlcNAc-(1->4)- $Mur2Ac(oyl-L-Ala-gamma-

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 8335642; 1459957 [H]