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The map label for this gene is dacB [H]

Identifier: 227815684

GI number: 227815684

Start: 2827488

End: 2828612

Strand: Reverse

Name: dacB [H]

Synonym: BAMEG_3104

Alternate gene names: 227815684

Gene position: 2828612-2827488 (Counterclockwise)

Preceding gene: 227815685

Following gene: 227815683

Centisome position: 54.08

GC content: 36.62

Gene sequence:

>1125_bases
ATGAGACGAATTTGTGTAATCATAACGCTTCTTATTATTTATGCAAGCGTTATGCCAATTCCTACATATGCAAAGATGAA
CAGCAATGTCAGTGCTCGTAATGCTGTATTAATGGAACAACATTCTGGACGTGTATTATACGGAAAAGCAGAACATGAGC
CGCAAAAAATTGCTAGTATAACAAAAATTATGACAGCCTTATTAGCTGCTGAATCAGGAAAGATGAAAGAAAGGGTATTA
GTAAGTAATGAAGCGGTCAGGGTCGAAGGATCTGCAATTTATTTAAAGCCTGGGCAAAAAGTGAAGCTAGAGGATTTAGT
ATACGGACTTATGCTTAGATCTGGTAATGACGCAGCACAAGTAATTGCTGAAAATGTAGGAGGGAGTATAGAAGGGTTCG
TATATTTAATGAATGAAAAGGCGAAACAGATTGGAATGAAAGATACTCACTTTTCAAACCCACATGGTTTGGATGGAGAT
GGATCTCATTATTCGTCGGCCTATGACATGGCACTTTTAACGAGATATGCAATGGGGAACGAGACTTTTAAGGAAATTTT
TGGAACAAAAACGTATAAATCAGATTCGTGGGATTATCCGTGGAAAAACAAACATAAACTTGTGACGTCGTATTATGAAT
TTGCAACAGGGGGAAAAACAGGTTTCACGAAGAAAGCAGGAAGAACGCTTGTTACAACAGCATCAAAAGATGGACTAGAT
CTAATTGTCGTAACTTTGAGCGCTTCTAGTGATTGGGATGATCATATGAATTTATTTGATAAAGGTTTTGACCGTTTCGA
ACAAACAAAAGTTTTAGGACAAGGAGCACTTGCTGAAATAACCGAAAAGAAATACGCCAATCATGTTTACACGAAAAATA
GTTTTTCAGTACCTTTAACTGAAGAGGAAAAAAAGAGCGTAGTATTAAAAGTTGAACTAGATAAAAGTGCAAAGCTTGTG
GATGGAGTAAAGGTTGGAAAGACAGACGTTTATGTTGGCAATGAGAAAGTTGGAGAACGGAATTTATTTTATAGTAAACG
AAAGTTAGTAGCTACAACCGGGATGTACTGGAATAATGTGAAAGAAATTTTTTCTTACATGATAGGTGTTGGTAGCGATG
GTTAA

Upstream 100 bases:

>100_bases
GCGTCTTTTTTATTGGAAGGAGAAGGAGATTTCGTTCATATTTTAAAAGGCCAAGCATACACTTGTACAAAAAGTGCCAA
AAGGACGTGGGGGGGGAAAT

Downstream 100 bases:

>100_bases
TCTCGTATGGGTAGCGATGGCAGTCATAGGGATTGTATATGCGATGATAAATGGAACGATGGAAGCAATAAATAAAGCTG
TATTTGATGGAGCGAAAGAT

Product: D-alanyl-D-alanine carboxypeptidase family protein

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5*; PBP-5* [H]

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MRRICVIITLLIIYASVMPIPTYAKMNSNVSARNAVLMEQHSGRVLYGKAEHEPQKIASITKIMTALLAAESGKMKERVL
VSNEAVRVEGSAIYLKPGQKVKLEDLVYGLMLRSGNDAAQVIAENVGGSIEGFVYLMNEKAKQIGMKDTHFSNPHGLDGD
GSHYSSAYDMALLTRYAMGNETFKEIFGTKTYKSDSWDYPWKNKHKLVTSYYEFATGGKTGFTKKAGRTLVTTASKDGLD
LIVVTLSASSDWDDHMNLFDKGFDRFEQTKVLGQGALAEITEKKYANHVYTKNSFSVPLTEEEKKSVVLKVELDKSAKLV
DGVKVGKTDVYVGNEKVGERNLFYSKRKLVATTGMYWNNVKEIFSYMIGVGSDG

Sequences:

>Translated_374_residues
MRRICVIITLLIIYASVMPIPTYAKMNSNVSARNAVLMEQHSGRVLYGKAEHEPQKIASITKIMTALLAAESGKMKERVL
VSNEAVRVEGSAIYLKPGQKVKLEDLVYGLMLRSGNDAAQVIAENVGGSIEGFVYLMNEKAKQIGMKDTHFSNPHGLDGD
GSHYSSAYDMALLTRYAMGNETFKEIFGTKTYKSDSWDYPWKNKHKLVTSYYEFATGGKTGFTKKAGRTLVTTASKDGLD
LIVVTLSASSDWDDHMNLFDKGFDRFEQTKVLGQGALAEITEKKYANHVYTKNSFSVPLTEEEKKSVVLKVELDKSAKLV
DGVKVGKTDVYVGNEKVGERNLFYSKRKLVATTGMYWNNVKEIFSYMIGVGSDG
>Mature_374_residues
MRRICVIITLLIIYASVMPIPTYAKMNSNVSARNAVLMEQHSGRVLYGKAEHEPQKIASITKIMTALLAAESGKMKERVL
VSNEAVRVEGSAIYLKPGQKVKLEDLVYGLMLRSGNDAAQVIAENVGGSIEGFVYLMNEKAKQIGMKDTHFSNPHGLDGD
GSHYSSAYDMALLTRYAMGNETFKEIFGTKTYKSDSWDYPWKNKHKLVTSYYEFATGGKTGFTKKAGRTLVTTASKDGLD
LIVVTLSASSDWDDHMNLFDKGFDRFEQTKVLGQGALAEITEKKYANHVYTKNSFSVPLTEEEKKSVVLKVELDKSAKLV
DGVKVGKTDVYVGNEKVGERNLFYSKRKLVATTGMYWNNVKEIFSYMIGVGSDG

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors. Required specifically for the synthesis of the spore form of peptidoglycan (cortex) [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Forespore outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI87082022, Length=372, Percent_Identity=29.8387096774194, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1787062, Length=284, Percent_Identity=34.1549295774648, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1786851, Length=262, Percent_Identity=33.206106870229, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI87082054, Length=211, Percent_Identity=26.0663507109005, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 41526; Mature: 41526

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRICVIITLLIIYASVMPIPTYAKMNSNVSARNAVLMEQHSGRVLYGKAEHEPQKIASI
CCHHHHHHHHHHHHHHHCCCCCHHHCCCCCCHHHEEEEECCCCEEEEECCCCCHHHHHHH
TKIMTALLAAESGKMKERVLVSNEAVRVEGSAIYLKPGQKVKLEDLVYGLMLRSGNDAAQ
HHHHHHHHHHCCCCCHHEEEECCCEEEEECCEEEECCCCEEEHHHHHHHHHHCCCCHHHH
VIAENVGGSIEGFVYLMNEKAKQIGMKDTHFSNPHGLDGDGSHYSSAYDMALLTRYAMGN
HHHHHCCCCCEEEEEEEECHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
ETFKEIFGTKTYKSDSWDYPWKNKHKLVTSYYEFATGGKTGFTKKAGRTLVTTASKDGLD
HHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHCCCEEEEECCCCCCE
LIVVTLSASSDWDDHMNLFDKGFDRFEQTKVLGQGALAEITEKKYANHVYTKNSFSVPLT
EEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEECCCCCCCCC
EEEKKSVVLKVELDKSAKLVDGVKVGKTDVYVGNEKVGERNLFYSKRKLVATTGMYWNNV
CCCCCEEEEEEEECCCCHHHCCEEECCEEEEECCCCCCCCCHHHHCCCEEEECCHHHHHH
KEIFSYMIGVGSDG
HHHHHHHHCCCCCC
>Mature Secondary Structure
MRRICVIITLLIIYASVMPIPTYAKMNSNVSARNAVLMEQHSGRVLYGKAEHEPQKIASI
CCHHHHHHHHHHHHHHHCCCCCHHHCCCCCCHHHEEEEECCCCEEEEECCCCCHHHHHHH
TKIMTALLAAESGKMKERVLVSNEAVRVEGSAIYLKPGQKVKLEDLVYGLMLRSGNDAAQ
HHHHHHHHHHCCCCCHHEEEECCCEEEEECCEEEECCCCEEEHHHHHHHHHHCCCCHHHH
VIAENVGGSIEGFVYLMNEKAKQIGMKDTHFSNPHGLDGDGSHYSSAYDMALLTRYAMGN
HHHHHCCCCCEEEEEEEECHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
ETFKEIFGTKTYKSDSWDYPWKNKHKLVTSYYEFATGGKTGFTKKAGRTLVTTASKDGLD
HHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHCCCEEEEECCCCCCE
LIVVTLSASSDWDDHMNLFDKGFDRFEQTKVLGQGALAEITEKKYANHVYTKNSFSVPLT
EEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEECCCCCCCCC
EEEKKSVVLKVELDKSAKLVDGVKVGKTDVYVGNEKVGERNLFYSKRKLVATTGMYWNNV
CCCCCEEEEEEEECCCCHHHCCEEECCEEEEECCCCCCCCCHHHHCCCEEEECCHHHHHH
KEIFSYMIGVGSDG
HHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1548223; 7934829; 9384377 [H]