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The map label for this gene is rluB [H]

Identifier: 227815681

GI number: 227815681

Start: 2825354

End: 2826082

Strand: Reverse

Name: rluB [H]

Synonym: BAMEG_3101

Alternate gene names: 227815681

Gene position: 2826082-2825354 (Counterclockwise)

Preceding gene: 227815682

Following gene: 227815680

Centisome position: 54.03

GC content: 38.68

Gene sequence:

>729_bases
ATGAAACGATTACAAAAAGTGATTGCGCAAGCAGGTATTGCATCGAGAAGAAAGGCTGAGGAATTAATTCAGCAAGGAAA
AGTGAAAGTTAACGGAAAAATAGTGAAGGAGTTAGGAACGAAAGTAACTCCTCAAGATAAAGTAGAAGTAAATAACATCC
CTCTTGAAAAAGAAGAACCTGTTTATTTTTTACTATATAAACCAACTGGCGTAATTTCAAGTGTATCAGATGATAAAGGA
AGAAGTGTTGTAACAGACTTTTTCCCAGAAATTACACAACGTTTATTCCCAATCGGACGTTTAGATTATGATACGTCTGG
CGTATTATTAATGACAAACGACGGGGACTTCGCAAACGTATTAATGCACCCTAGATATAAAGTTGAAAAAACATATGTTG
CAAAAATAAAAGGGCCTCTAACAGGTGAAAAAATCCGCATGTTAGAACGTGGTGTGACGTTAGAAGACGGAAAAACAGCA
CCAGCGCGTGTGAAGATTATTTCTTGGGACAAGCGTAAAGAAATGGCGATTGTACAACTAACAATTCATGAAGGACGTAA
CCGTCAAGTACGTCGTATGTTTGAAGCTTTAGACTGTAAAGTAGTGAAACTAAAGCGTGAACGCTACGCCTTTTTAGAAG
TGGGTAGCTTGCGACCTGGTGATGCAAGAGAATTGACACCACATGAGGTGAAACAATTACGTGCATTGGCTTCCACAAAG
CCTCGCTAA

Upstream 100 bases:

>100_bases
CTATGTTGTTTAAGTAAGATGGAGCGAAAAGCCGTCAAGTGTATGCGGTTGCCAAGTCTTTCTAATGTAAGGTAAGATAA
ATTCGAGGTGAAAAAAAGAA

Downstream 100 bases:

>100_bases
TCGATATTGTGAAACTTTCACCAGTGGAATGTTCCGCTGGTGATTTATTAAAAAATACATATATTCATCACTCGTCGAAG
AGAGGGGAAACAATATGAAG

Product: ribosomal large subunit pseudouridine synthase B

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MKRLQKVIAQAGIASRRKAEELIQQGKVKVNGKIVKELGTKVTPQDKVEVNNIPLEKEEPVYFLLYKPTGVISSVSDDKG
RSVVTDFFPEITQRLFPIGRLDYDTSGVLLMTNDGDFANVLMHPRYKVEKTYVAKIKGPLTGEKIRMLERGVTLEDGKTA
PARVKIISWDKRKEMAIVQLTIHEGRNRQVRRMFEALDCKVVKLKRERYAFLEVGSLRPGDARELTPHEVKQLRALASTK
PR

Sequences:

>Translated_242_residues
MKRLQKVIAQAGIASRRKAEELIQQGKVKVNGKIVKELGTKVTPQDKVEVNNIPLEKEEPVYFLLYKPTGVISSVSDDKG
RSVVTDFFPEITQRLFPIGRLDYDTSGVLLMTNDGDFANVLMHPRYKVEKTYVAKIKGPLTGEKIRMLERGVTLEDGKTA
PARVKIISWDKRKEMAIVQLTIHEGRNRQVRRMFEALDCKVVKLKRERYAFLEVGSLRPGDARELTPHEVKQLRALASTK
PR
>Mature_242_residues
MKRLQKVIAQAGIASRRKAEELIQQGKVKVNGKIVKELGTKVTPQDKVEVNNIPLEKEEPVYFLLYKPTGVISSVSDDKG
RSVVTDFFPEITQRLFPIGRLDYDTSGVLLMTNDGDFANVLMHPRYKVEKTYVAKIKGPLTGEKIRMLERGVTLEDGKTA
PARVKIISWDKRKEMAIVQLTIHEGRNRQVRRMFEALDCKVVKLKRERYAFLEVGSLRPGDARELTPHEVKQLRALASTK
PR

Specific function: Responsible for synthesis of pseudouridine from uracil- 2633 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=249, Percent_Identity=39.3574297188755, Blast_Score=145, Evalue=2e-36,
Organism=Escherichia coli, GI1788510, Length=236, Percent_Identity=36.864406779661, Blast_Score=137, Evalue=9e-34,
Organism=Escherichia coli, GI1790453, Length=237, Percent_Identity=35.8649789029536, Blast_Score=127, Evalue=6e-31,
Organism=Escherichia coli, GI87081838, Length=184, Percent_Identity=31.5217391304348, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 27526; Mature: 27526

Theoretical pI: Translated: 10.55; Mature: 10.55

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLQKVIAQAGIASRRKAEELIQQGKVKVNGKIVKELGTKVTPQDKVEVNNIPLEKEEP
CHHHHHHHHHHCCHHHHHHHHHHHCCCEEECCEEHHHCCCCCCCCCCEEECCCCCCCCCC
VYFLLYKPTGVISSVSDDKGRSVVTDFFPEITQRLFPIGRLDYDTSGVLLMTNDGDFANV
EEEEEECCCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCEEEE
LMHPRYKVEKTYVAKIKGPLTGEKIRMLERGVTLEDGKTAPARVKIISWDKRKEMAIVQL
EECCCEEECCEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCEEEEEE
TIHEGRNRQVRRMFEALDCKVVKLKRERYAFLEVGSLRPGDARELTPHEVKQLRALASTK
EEECCCCHHHHHHHHHHCCEEEEEHHHHEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCC
PR
CC
>Mature Secondary Structure
MKRLQKVIAQAGIASRRKAEELIQQGKVKVNGKIVKELGTKVTPQDKVEVNNIPLEKEEP
CHHHHHHHHHHCCHHHHHHHHHHHCCCEEECCEEHHHCCCCCCCCCCEEECCCCCCCCCC
VYFLLYKPTGVISSVSDDKGRSVVTDFFPEITQRLFPIGRLDYDTSGVLLMTNDGDFANV
EEEEEECCCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCEEEE
LMHPRYKVEKTYVAKIKGPLTGEKIRMLERGVTLEDGKTAPARVKIISWDKRKEMAIVQL
EECCCEEECCEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCEEEEEE
TIHEGRNRQVRRMFEALDCKVVKLKRERYAFLEVGSLRPGDARELTPHEVKQLRALASTK
EEECCCCHHHHHHHHHHCCEEEEEHHHHEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCC
PR
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934829; 9384377; 9888802 [H]