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The map label for this gene is panB

Identifier: 227815614

GI number: 227815614

Start: 2766584

End: 2767423

Strand: Reverse

Name: panB

Synonym: BAMEG_3033

Alternate gene names: 227815614

Gene position: 2767423-2766584 (Counterclockwise)

Preceding gene: 227815616

Following gene: 227815613

Centisome position: 52.91

GC content: 41.19

Gene sequence:

>840_bases
TTGAAAACAAAAACAGATTTTTTGAAAATGAAAGAGCAAGGTGAGCCGATTACAATGCTAACGGCGTATGATTATCCTTC
TGCTAAATTAGCAGAAGAAGCTGAAGTTGATATGATTCTAGTTGGAGATTCTCTCGGGATGGTTGTGCTCGGCTACGATT
CAACAGTACCAGTAACAGTAGAGGATATGATTCATCATACAAAAGCTGTACGCCGCGGAGCGAAAGAGACGTTTATTGTA
ACTGATATGCCATTTATGTCGTATCATGTGTCACTGCAAGATACGATGGTGAATGCACGCCGCATCGTTCAAGAGAGTGG
TGCCCATGCACTGAAGGTAGAAGGTGCGGGAGAAGTGATATCGACTATTCACTATTTGACGAATGCGGGAATTCCTGTCG
TGGCGCACTTAGGTTTAACTCCTCAATCTGTAGGAGTGCTAGGTGGATATAAAGTACAAGGAAAAGACGCTGAAAGTGCA
AAAAAATTAATAGAAGATGCGAAGAAATGTGAGGAAGCTGGTGCAATTGCACTCGTGTTAGAGTGTGTGCCAATGCAGTT
AGCAGAACTTATTTCAGAGCAATTAACAATTCCAACAATCGGGATTGGCGCAGGACAAAAAGTAGATGGGCAAGTGCTTG
TTTATCATGATCTTATCTCATATGGGGTAAATCGTGTTCCGAAATTTGTGAAGCAATATACGTCCGTTCAAGAAGAAATT
GTACGCGGGATTTCTCAATATGTTGCTGAAGTAAAGACAAGGCAATTTCCTGAAGAAAAACATTCGTTCACAATGAAAGA
GGAAGAATGCTTAGCGTTATACGGAGGAAAACAATCATGA

Upstream 100 bases:

>100_bases
ATGCCGACTAACACACAACCCTTCTGCCCTTTTATGGCCAGAGGGGTTTTTTATATGATTTCGGCCATCTCCTCTCTCCT
GTATAAAGGAGGAGTAGTTT

Downstream 100 bases:

>100_bases
AAATCGTAACGACAGTACAAGAGATGCAGCACATTACAAAAGAACTGCGTGCAAGTGGAAAAAGTATTGGTTTTGTCCCA
ACGATGGGGTATTTACATGA

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MKTKTDFLKMKEQGEPITMLTAYDYPSAKLAEEAEVDMILVGDSLGMVVLGYDSTVPVTVEDMIHHTKAVRRGAKETFIV
TDMPFMSYHVSLQDTMVNARRIVQESGAHALKVEGAGEVISTIHYLTNAGIPVVAHLGLTPQSVGVLGGYKVQGKDAESA
KKLIEDAKKCEEAGAIALVLECVPMQLAELISEQLTIPTIGIGAGQKVDGQVLVYHDLISYGVNRVPKFVKQYTSVQEEI
VRGISQYVAEVKTRQFPEEKHSFTMKEEECLALYGGKQS

Sequences:

>Translated_279_residues
MKTKTDFLKMKEQGEPITMLTAYDYPSAKLAEEAEVDMILVGDSLGMVVLGYDSTVPVTVEDMIHHTKAVRRGAKETFIV
TDMPFMSYHVSLQDTMVNARRIVQESGAHALKVEGAGEVISTIHYLTNAGIPVVAHLGLTPQSVGVLGGYKVQGKDAESA
KKLIEDAKKCEEAGAIALVLECVPMQLAELISEQLTIPTIGIGAGQKVDGQVLVYHDLISYGVNRVPKFVKQYTSVQEEI
VRGISQYVAEVKTRQFPEEKHSFTMKEEECLALYGGKQS
>Mature_279_residues
MKTKTDFLKMKEQGEPITMLTAYDYPSAKLAEEAEVDMILVGDSLGMVVLGYDSTVPVTVEDMIHHTKAVRRGAKETFIV
TDMPFMSYHVSLQDTMVNARRIVQESGAHALKVEGAGEVISTIHYLTNAGIPVVAHLGLTPQSVGVLGGYKVQGKDAESA
KKLIEDAKKCEEAGAIALVLECVPMQLAELISEQLTIPTIGIGAGQKVDGQVLVYHDLISYGVNRVPKFVKQYTSVQEEI
VRGISQYVAEVKTRQFPEEKHSFTMKEEECLALYGGKQS

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=255, Percent_Identity=46.6666666666667, Blast_Score=239, Evalue=2e-64,
Organism=Saccharomyces cerevisiae, GI6319653, Length=263, Percent_Identity=40.6844106463878, Blast_Score=207, Evalue=2e-54,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_BACAA (C3P5Q9)

Other databases:

- EMBL:   CP001598
- RefSeq:   YP_002866042.1
- ProteinModelPortal:   C3P5Q9
- SMR:   C3P5Q9
- EnsemblBacteria:   EBBACT00000130546
- GeneID:   7848037
- GenomeReviews:   CP001598_GR
- KEGG:   bai:BAA_1629
- GeneTree:   EBGT00050000000505
- ProtClustDB:   PRK00311
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 30536; Mature: 30536

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: NA

Important sites: ACT_SITE 181-181 BINDING 82-82 BINDING 112-112

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTKTDFLKMKEQGEPITMLTAYDYPSAKLAEEAEVDMILVGDSLGMVVLGYDSTVPVTV
CCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHCCCCCEEEEECCCCEEEEECCCCCCEEH
EDMIHHTKAVRRGAKETFIVTDMPFMSYHVSLQDTMVNARRIVQESGAHALKVEGAGEVI
HHHHHHHHHHHCCCCCEEEEECCCHHHEEEEHHHHHHHHHHHHHHCCCCEEEECCCHHHH
STIHYLTNAGIPVVAHLGLTPQSVGVLGGYKVQGKDAESAKKLIEDAKKCEEAGAIALVL
HHHHHHHCCCCCEEEECCCCHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHCCHHHHHH
ECVPMQLAELISEQLTIPTIGIGAGQKVDGQVLVYHDLISYGVNRVPKFVKQYTSVQEEI
HHHHHHHHHHHHHCCCCCEEECCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
VRGISQYVAEVKTRQFPEEKHSFTMKEEECLALYGGKQS
HHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHEECCCCCC
>Mature Secondary Structure
MKTKTDFLKMKEQGEPITMLTAYDYPSAKLAEEAEVDMILVGDSLGMVVLGYDSTVPVTV
CCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHCCCCCEEEEECCCCEEEEECCCCCCEEH
EDMIHHTKAVRRGAKETFIVTDMPFMSYHVSLQDTMVNARRIVQESGAHALKVEGAGEVI
HHHHHHHHHHHCCCCCEEEEECCCHHHEEEEHHHHHHHHHHHHHHCCCCEEEECCCHHHH
STIHYLTNAGIPVVAHLGLTPQSVGVLGGYKVQGKDAESAKKLIEDAKKCEEAGAIALVL
HHHHHHHCCCCCEEEECCCCHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHCCHHHHHH
ECVPMQLAELISEQLTIPTIGIGAGQKVDGQVLVYHDLISYGVNRVPKFVKQYTSVQEEI
HHHHHHHHHHHHHCCCCCEEECCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
VRGISQYVAEVKTRQFPEEKHSFTMKEEECLALYGGKQS
HHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA