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The map label for this gene is chrR [H]

Identifier: 227815530

GI number: 227815530

Start: 2679195

End: 2679929

Strand: Reverse

Name: chrR [H]

Synonym: BAMEG_2943

Alternate gene names: 227815530

Gene position: 2679929-2679195 (Counterclockwise)

Preceding gene: 227815531

Following gene: 227815529

Centisome position: 51.24

GC content: 36.73

Gene sequence:

>735_bases
ATGAATGAAATGATACATAAAATGGAGCAACACGTTTCAGTTCGTAAATATAAAGAAGAGTCCATTCCGAAAGATGTAGT
AGAAAGAATGGTGCAAGCTGGGCAGCATGCTGCGTCCTCTCATTTTGTACAGGCCTATTCTGTTATATATGTAACCGATC
AAGACTTAAAGGCTAAACTAGCAGAGTTGTCTGGAAATCGACATGTGAAAGATTGCGCAGCATTCTTTGTTTGTTGTGCA
GATTTAAAACGACTTGAAATGGCATGTGAAAAACATGGTACAGAGATAAAGCATGAAGGAGTAGAAGACTTTATCGTAGC
AACGGTAGATGCTTCACTTTTTGCGCAAAACTTAGCGTTAGCAGCAGAATCGTTAGGATACGGTATTTGTTATATTGGTG
GTATTCGTAATAATCCGAGAGAAGTGAGCGAATTGCTTCATTTACCGGATAAAGTATATCCTGTATTCGGAATGACAGTC
GGTGTACCAAATGAAGATCATGGAGTAAAACCACGCTTACCAGTAGCAGCAATCCTTCATGAAAATGAATATGATGAAAA
GAAATATGATGAATTACTAAACGAATACGATGAAACGATGAGCGCGTATTATAAAGGAAGATCATCAAACAAGAAAAGCG
TAACATGGACGGAATCAATGAGTTCATTTATGTCTAAAGAAAAAAGAATGCACATGAAAGAGTTTTTAAATGAGAGAGGA
TTAAATAAGAAATGA

Upstream 100 bases:

>100_bases
AGAGCAATTCAAAAGAATTGCTCTTTTTGTGCTTATAAGTTGTGAATATTGGAAAAATATGTTAATTTCTCATTAGGAAG
AGAAAAAAGGAGTGATTTTC

Downstream 100 bases:

>100_bases
GTAAAAAGGCAGACCTAAATGTAGGTTTGCCTTTTTTGGGAGGAGATAATATGAAAAAAGCTTTAATCTTTTTATTTGAT
GGATTTGCAGAATTTGAAGT

Product: nitroreductase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MNEMIHKMEQHVSVRKYKEESIPKDVVERMVQAGQHAASSHFVQAYSVIYVTDQDLKAKLAELSGNRHVKDCAAFFVCCA
DLKRLEMACEKHGTEIKHEGVEDFIVATVDASLFAQNLALAAESLGYGICYIGGIRNNPREVSELLHLPDKVYPVFGMTV
GVPNEDHGVKPRLPVAAILHENEYDEKKYDELLNEYDETMSAYYKGRSSNKKSVTWTESMSSFMSKEKRMHMKEFLNERG
LNKK

Sequences:

>Translated_244_residues
MNEMIHKMEQHVSVRKYKEESIPKDVVERMVQAGQHAASSHFVQAYSVIYVTDQDLKAKLAELSGNRHVKDCAAFFVCCA
DLKRLEMACEKHGTEIKHEGVEDFIVATVDASLFAQNLALAAESLGYGICYIGGIRNNPREVSELLHLPDKVYPVFGMTV
GVPNEDHGVKPRLPVAAILHENEYDEKKYDELLNEYDETMSAYYKGRSSNKKSVTWTESMSSFMSKEKRMHMKEFLNERG
LNKK
>Mature_244_residues
MNEMIHKMEQHVSVRKYKEESIPKDVVERMVQAGQHAASSHFVQAYSVIYVTDQDLKAKLAELSGNRHVKDCAAFFVCCA
DLKRLEMACEKHGTEIKHEGVEDFIVATVDASLFAQNLALAAESLGYGICYIGGIRNNPREVSELLHLPDKVYPVFGMTV
GVPNEDHGVKPRLPVAAILHENEYDEKKYDELLNEYDETMSAYYKGRSSNKKSVTWTESMSSFMSKEKRMHMKEFLNERG
LNKK

Specific function: Reduction Of Nitroaromatic Compounds Using NADH. Reduces Nitrofurazone By A Ping-Pong Bi-Bi Mechanism Possibly To Generate A Two-Electron Transfer Product. Major Component Of The Oxygen- Insensitive Nitroreductase Activity In E.Coli. [C]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the flavin oxidoreductase frp family [H]

Homologues:

Organism=Escherichia coli, GI1787075, Length=242, Percent_Identity=35.9504132231405, Blast_Score=152, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016446
- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 27756; Mature: 27756

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEMIHKMEQHVSVRKYKEESIPKDVVERMVQAGQHAASSHFVQAYSVIYVTDQDLKAKL
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHEEEEEEECHHHHHHH
AELSGNRHVKDCAAFFVCCADLKRLEMACEKHGTEIKHEGVEDFIVATVDASLFAQNLAL
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHHHHH
AAESLGYGICYIGGIRNNPREVSELLHLPDKVYPVFGMTVGVPNEDHGVKPRLPVAAILH
HHHHHCCCEEEECCCCCCHHHHHHHHHCCHHHHHHHHEEECCCCCCCCCCCCCCHHHHHH
ENEYDEKKYDELLNEYDETMSAYYKGRSSNKKSVTWTESMSSFMSKEKRMHMKEFLNERG
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCC
LNKK
CCCC
>Mature Secondary Structure
MNEMIHKMEQHVSVRKYKEESIPKDVVERMVQAGQHAASSHFVQAYSVIYVTDQDLKAKL
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHEEEEEEECHHHHHHH
AELSGNRHVKDCAAFFVCCADLKRLEMACEKHGTEIKHEGVEDFIVATVDASLFAQNLAL
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHHHHH
AAESLGYGICYIGGIRNNPREVSELLHLPDKVYPVFGMTVGVPNEDHGVKPRLPVAAILH
HHHHHCCCEEEECCCCCCHHHHHHHHHCCHHHHHHHHEEECCCCCCCCCCCCCCHHHHHH
ENEYDEKKYDELLNEYDETMSAYYKGRSSNKKSVTWTESMSSFMSKEKRMHMKEFLNERG
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCC
LNKK
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA