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The map label for this gene is brnQ [H]

Identifier: 227815270

GI number: 227815270

Start: 2441068

End: 2442018

Strand: Reverse

Name: brnQ [H]

Synonym: BAMEG_2681

Alternate gene names: 227815270

Gene position: 2442018-2441068 (Counterclockwise)

Preceding gene: 227815271

Following gene: 227815269

Centisome position: 46.69

GC content: 35.02

Gene sequence:

>951_bases
TTGTTTTTTTCGCTAAATCCCGCGAAAATTGTCGATATTGTTGGAAAAATCTTAACGCCAATTAAGTTAACTTTCATCGG
TATTTTAGTAATCGTTGCTTTTATTCATCCGATTGGAGAAATGCAAGCACCAGTTGAAGCTTATACATCACATGCATTCT
TTAAAGGGTTCCAAGAAGGATACTTAACGATGGACACGCTTGCATCATTCGTATTCGGAATCATCATCATTAATGCAATT
AAAGAAAAAGGTGCAAAAACGAAAACACAAATTATGGTCGTTTGTGCAAAAGCAACAATCATTGCAGCATCTATTTTAGC
AATTATCTATACAGCACTTTCTTATATGGGTGCTTCAAGTGTTGCAAAGCTTGGACATTTAGAGAACGGCGGAGAAGTAT
TAGCGAAAGTTTCTAACTACTATTTTGGATCATATGGCGGAGTATTATTAGGGTTAATGATTACAGTAGCTTGTTTAACA
ACTAGTGTGGGACTTGTATCAGCATGTTCTTCATTCTTCCATAAGTTATTCCCAAATGTTCCTTACAAAGCAATTGCAAT
CACGCTATGTATATTTAGTGCAATTGTTGCAAATGTAGGATTAACACAATTAATTGCAGTTTCTGTTCCAGTATTAACAG
CAATTTATCCACTAGCAATCGTATTGATTTTCTTAACATTCTTCCATTCATTATTCAAAGGAAGAGCTGAAGTTTATCAA
GTGAGCTTAACTGTAACATTTATTATCAGCTTATTCGATGGATTAAGTGCAGCTGGAGTTAACGTTGAAGTAGTAAGCCA
AGTGTTCACTAAATTCCTTCCGATGCAGGAAGTAGGATTAGGCTGGATCTTCCCAGCGATTATCGGTGGATTTATCGGAT
ATGGCATTAGCGTTTTAAAATTAAAAAATCAAATTCAACCAGCAGCAAATACGAATAAGAAAATAGGTTAA

Upstream 100 bases:

>100_bases
CAGGAAATGTTTCTTATGAAATTGGTCTTAAACCGTTTATGCCAGAGGGATTAGGTTCTACACCTTTAATTCTTTTCACA
ATTGTATTCTTTAGCATCAC

Downstream 100 bases:

>100_bases
ATAAAAAAGTTATAGAACTTGTTTCTATAGCTTTTTTTGTTACTATTAATAATGAAAGAAAATAAAGTAGTAGGGGAAAT
AAAATGAAAAAAACAATTGA

Product: branched-chain amino acid transport system II carrier protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: Branched-chain amino acid uptake carrier BrnQ [H]

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MFFSLNPAKIVDIVGKILTPIKLTFIGILVIVAFIHPIGEMQAPVEAYTSHAFFKGFQEGYLTMDTLASFVFGIIIINAI
KEKGAKTKTQIMVVCAKATIIAASILAIIYTALSYMGASSVAKLGHLENGGEVLAKVSNYYFGSYGGVLLGLMITVACLT
TSVGLVSACSSFFHKLFPNVPYKAIAITLCIFSAIVANVGLTQLIAVSVPVLTAIYPLAIVLIFLTFFHSLFKGRAEVYQ
VSLTVTFIISLFDGLSAAGVNVEVVSQVFTKFLPMQEVGLGWIFPAIIGGFIGYGISVLKLKNQIQPAANTNKKIG

Sequences:

>Translated_316_residues
MFFSLNPAKIVDIVGKILTPIKLTFIGILVIVAFIHPIGEMQAPVEAYTSHAFFKGFQEGYLTMDTLASFVFGIIIINAI
KEKGAKTKTQIMVVCAKATIIAASILAIIYTALSYMGASSVAKLGHLENGGEVLAKVSNYYFGSYGGVLLGLMITVACLT
TSVGLVSACSSFFHKLFPNVPYKAIAITLCIFSAIVANVGLTQLIAVSVPVLTAIYPLAIVLIFLTFFHSLFKGRAEVYQ
VSLTVTFIISLFDGLSAAGVNVEVVSQVFTKFLPMQEVGLGWIFPAIIGGFIGYGISVLKLKNQIQPAANTNKKIG
>Mature_316_residues
MFFSLNPAKIVDIVGKILTPIKLTFIGILVIVAFIHPIGEMQAPVEAYTSHAFFKGFQEGYLTMDTLASFVFGIIIINAI
KEKGAKTKTQIMVVCAKATIIAASILAIIYTALSYMGASSVAKLGHLENGGEVLAKVSNYYFGSYGGVLLGLMITVACLT
TSVGLVSACSSFFHKLFPNVPYKAIAITLCIFSAIVANVGLTQLIAVSVPVLTAIYPLAIVLIFLTFFHSLFKGRAEVYQ
VSLTVTFIISLFDGLSAAGVNVEVVSQVFTKFLPMQEVGLGWIFPAIIGGFIGYGISVLKLKNQIQPAANTNKKIG

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine) Which is coupled to a proton motive force [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=285, Percent_Identity=36.8421052631579, Blast_Score=191, Evalue=8e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 33769; Mature: 33769

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFFSLNPAKIVDIVGKILTPIKLTFIGILVIVAFIHPIGEMQAPVEAYTSHAFFKGFQEG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHC
YLTMDTLASFVFGIIIINAIKEKGAKTKTQIMVVCAKATIIAASILAIIYTALSYMGASS
CEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHH
VAKLGHLENGGEVLAKVSNYYFGSYGGVLLGLMITVACLTTSVGLVSACSSFFHKLFPNV
HHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PYKAIAITLCIFSAIVANVGLTQLIAVSVPVLTAIYPLAIVLIFLTFFHSLFKGRAEVYQ
CHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
VSLTVTFIISLFDGLSAAGVNVEVVSQVFTKFLPMQEVGLGWIFPAIIGGFIGYGISVLK
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHCHHHHHHHHHHHHHHHHHHHHH
LKNQIQPAANTNKKIG
HHHHCCCCCCCCCCCC
>Mature Secondary Structure
MFFSLNPAKIVDIVGKILTPIKLTFIGILVIVAFIHPIGEMQAPVEAYTSHAFFKGFQEG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHC
YLTMDTLASFVFGIIIINAIKEKGAKTKTQIMVVCAKATIIAASILAIIYTALSYMGASS
CEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHH
VAKLGHLENGGEVLAKVSNYYFGSYGGVLLGLMITVACLTTSVGLVSACSSFFHKLFPNV
HHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PYKAIAITLCIFSAIVANVGLTQLIAVSVPVLTAIYPLAIVLIFLTFFHSLFKGRAEVYQ
CHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
VSLTVTFIISLFDGLSAAGVNVEVVSQVFTKFLPMQEVGLGWIFPAIIGGFIGYGISVLK
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHCHHHHHHHHHHHHHHHHHHHHH
LKNQIQPAANTNKKIG
HHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9023181; 9308178; 9384377 [H]