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The map label for this gene is dacA [H]

Identifier: 227815230

GI number: 227815230

Start: 2402659

End: 2403516

Strand: Reverse

Name: dacA [H]

Synonym: BAMEG_2641

Alternate gene names: 227815230

Gene position: 2403516-2402659 (Counterclockwise)

Preceding gene: 227815232

Following gene: 227815229

Centisome position: 45.96

GC content: 36.01

Gene sequence:

>858_bases
ATGAAGCGGTTAAAATGGGGAAGGATAACGATCCTATTAGTAATTATTTTAGGGATATGTTGGTTCTTATTCCAAGGAAC
TCAAAAGAGTGTAACAAATGTAGAGGGACAACCTTTACAAGTGGCAGAACTACCTAAAACAGGATTAACTGAAAAAGTAG
TGCCACCAAAGTATATACCACCGGAAGTTGATGCAAAAGCAGCTATGATTATTGATGCGAGTAATGGAGATATTATTTAT
CAACATAATGAAAATGAGGCTTTTGCGCCAGCGGCACAAACAGAAGGGGCAAGAATCCCAGTCCAAGTGAATGACACATT
GACTGTTAAAGATTTGTTCCATGCATTAATGATTGAATCAGCAAATAATTCGGCTGTAGCTTTAGCAGAACATATGGCAA
AGACGGAGAAAGATTTCGTTCAGCTTATGAATGCAAAAGCGAAGCAGTTAAAAATGTCGGATTATGCAAAATTCGCAAAT
GCATCAGGACTACAGGAGCCCGATGGAAGTGAGACGAAGATGACAGCTGCTGATGTAGCAAACTTGGCGTATCATCTTAT
AAAAGATTACCCAGAAATATTAGAAGTGACTCACTTACGTCAAAGTCAGTTAGCATTTAATAATATTAATGTTATAAGTA
CAAATGATATGTTAAACAAAAATAATAAAGCTTTATATATAGAAGGAATAGACGGATTAAAAACAGGATTCACAGATAGT
GCAGGGTATTGTTTTACGGGTACAGCAAAACAAGGTGATACTCGTATTATTACGGTTGTAATGGGAACGAAAGGTAAAAC
GAAACGTTTTACAGAGACAAATAAGCTAATGACTTATGCATTTGGCTTAGTTAATTAA

Upstream 100 bases:

>100_bases
GTTATAGATGAATAAATAGACTTTTTATCTTGACGATATAAAGGGAAAGTTGTTATGGTTGAATGTGAGTGTATAAAAGG
TTTGCTAAAGGAGTTTTGAA

Downstream 100 bases:

>100_bases
GTGAAATACTGAGTTGGATCATCATTATATGAAAGGCGATTTATTGTTACGTATTATCAGTAACAATAAATCGCCTTTTT
ATTTTTAATAGTAGGAAATA

Product: D-alanyl-D-alanine carboxypeptidase family protein

Products: NA

Alternate protein names: CPase; DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MKRLKWGRITILLVIILGICWFLFQGTQKSVTNVEGQPLQVAELPKTGLTEKVVPPKYIPPEVDAKAAMIIDASNGDIIY
QHNENEAFAPAAQTEGARIPVQVNDTLTVKDLFHALMIESANNSAVALAEHMAKTEKDFVQLMNAKAKQLKMSDYAKFAN
ASGLQEPDGSETKMTAADVANLAYHLIKDYPEILEVTHLRQSQLAFNNINVISTNDMLNKNNKALYIEGIDGLKTGFTDS
AGYCFTGTAKQGDTRIITVVMGTKGKTKRFTETNKLMTYAFGLVN

Sequences:

>Translated_285_residues
MKRLKWGRITILLVIILGICWFLFQGTQKSVTNVEGQPLQVAELPKTGLTEKVVPPKYIPPEVDAKAAMIIDASNGDIIY
QHNENEAFAPAAQTEGARIPVQVNDTLTVKDLFHALMIESANNSAVALAEHMAKTEKDFVQLMNAKAKQLKMSDYAKFAN
ASGLQEPDGSETKMTAADVANLAYHLIKDYPEILEVTHLRQSQLAFNNINVISTNDMLNKNNKALYIEGIDGLKTGFTDS
AGYCFTGTAKQGDTRIITVVMGTKGKTKRFTETNKLMTYAFGLVN
>Mature_285_residues
MKRLKWGRITILLVIILGICWFLFQGTQKSVTNVEGQPLQVAELPKTGLTEKVVPPKYIPPEVDAKAAMIIDASNGDIIY
QHNENEAFAPAAQTEGARIPVQVNDTLTVKDLFHALMIESANNSAVALAEHMAKTEKDFVQLMNAKAKQLKMSDYAKFAN
ASGLQEPDGSETKMTAADVANLAYHLIKDYPEILEVTHLRQSQLAFNNINVISTNDMLNKNNKALYIEGIDGLKTGFTDS
AGYCFTGTAKQGDTRIITVVMGTKGKTKRFTETNKLMTYAFGLVN

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted, cell wall [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=259, Percent_Identity=30.1158301158301, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI87082022, Length=272, Percent_Identity=28.3088235294118, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1786851, Length=259, Percent_Identity=27.027027027027, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI87082054, Length=255, Percent_Identity=27.843137254902, Blast_Score=81, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 31347; Mature: 31347

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLKWGRITILLVIILGICWFLFQGTQKSVTNVEGQPLQVAELPKTGLTEKVVPPKYIP
CCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCC
PEVDAKAAMIIDASNGDIIYQHNENEAFAPAAQTEGARIPVQVNDTLTVKDLFHALMIES
CCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCCCCCEEEEEECCCEEHHHHHHHHHHCC
ANNSAVALAEHMAKTEKDFVQLMNAKAKQLKMSDYAKFANASGLQEPDGSETKMTAADVA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHH
NLAYHLIKDYPEILEVTHLRQSQLAFNNINVISTNDMLNKNNKALYIEGIDGLKTGFTDS
HHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEECCCHHCCCCCEEEEECCCCCCCCCCCC
AGYCFTGTAKQGDTRIITVVMGTKGKTKRFTETNKLMTYAFGLVN
CCEEEECCCCCCCCEEEEEEECCCCCCCCHHHHHHHEEHHEECCC
>Mature Secondary Structure
MKRLKWGRITILLVIILGICWFLFQGTQKSVTNVEGQPLQVAELPKTGLTEKVVPPKYIP
CCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCC
PEVDAKAAMIIDASNGDIIYQHNENEAFAPAAQTEGARIPVQVNDTLTVKDLFHALMIES
CCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCCCCCEEEEEECCCEEHHHHHHHHHHCC
ANNSAVALAEHMAKTEKDFVQLMNAKAKQLKMSDYAKFANASGLQEPDGSETKMTAADVA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHH
NLAYHLIKDYPEILEVTHLRQSQLAFNNINVISTNDMLNKNNKALYIEGIDGLKTGFTDS
HHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEECCCHHCCCCCEEEEECCCCCCCCCCCC
AGYCFTGTAKQGDTRIITVVMGTKGKTKRFTETNKLMTYAFGLVN
CCEEEECCCCCCCCEEEEEEECCCCCCCCHHHHHHHEEHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8370539; 6780559; 111240 [H]