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The map label for this gene is lcfA [H]

Identifier: 227815196

GI number: 227815196

Start: 2369446

End: 2370084

Strand: Reverse

Name: lcfA [H]

Synonym: BAMEG_2607

Alternate gene names: 227815196

Gene position: 2370084-2369446 (Counterclockwise)

Preceding gene: 227815197

Following gene: 227815195

Centisome position: 45.32

GC content: 36.78

Gene sequence:

>639_bases
ATGCTACATATTATGGGGAGTTTTCCGCAAGGAACGTTTCAGTTTCATAAAATTATGACATCCGGAGCACCTTTGCCAGA
AGCATTATTTTATAAACTAAAAGAAACGACAACATATATGATGCAACAATACGGTTGTTCTGAAGCAGGTTGTATTAGTA
TATGTCATGATATGAAAAGCCATTTAGATTTAGGAAACCCGCTACCTCATGCGAGTATAAGTATAGGTTCAGATGAAAAT
GCACCGGAAGAAATCATAGTGAAAATGAATGATAAGGAAATTTTCACGAAAGATTTAGGGTATAAATCTGAGCGTGGGCT
TCATTTTATGGGGCGTATGGACGATGTGATTAACGTTTCAGGATTAAAAGTGTTTCCTATAGAGGTAGAAGAAACGATGC
TTCGATTGGAAGGTGTGCAAGAGGCGATTGTATATCGAGGGAAACATCCTGTCATGGGGGAAATTGTTAAAGCGAAAGTA
ATCTCTCATATTGATCCAGTTCAAATAAGAGAATGGTGTATGCAGCATTTACCATCTTATAAAGTTCCGCATGAGATTGA
AAGTGTAACTGAAATTCCGAAAAATAAAACTGGAAAAGTAAGTAGAAAGTTACTAGAGATGGGAGAGGTTACAACATGA

Upstream 100 bases:

>100_bases
AGGGGGAAGCAAACCTATCATCATTACGAATAAAAACCCTAAATTCGCATTAAATATAGTTCGCAATACAGAAAAACATA
TCGTATATGCAGTACCACTT

Downstream 100 bases:

>100_bases
GACGGGAAGCGTTAAAGAATGCTGTATTAAAAATTATGACAGAAAAAATGGAACTGAAAAATGTAACGCATTTAGAAGAA
ACGATGCGTTTAAATCAAGA

Product: acyl-CoA synthetase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MLHIMGSFPQGTFQFHKIMTSGAPLPEALFYKLKETTTYMMQQYGCSEAGCISICHDMKSHLDLGNPLPHASISIGSDEN
APEEIIVKMNDKEIFTKDLGYKSERGLHFMGRMDDVINVSGLKVFPIEVEETMLRLEGVQEAIVYRGKHPVMGEIVKAKV
ISHIDPVQIREWCMQHLPSYKVPHEIESVTEIPKNKTGKVSRKLLEMGEVTT

Sequences:

>Translated_212_residues
MLHIMGSFPQGTFQFHKIMTSGAPLPEALFYKLKETTTYMMQQYGCSEAGCISICHDMKSHLDLGNPLPHASISIGSDEN
APEEIIVKMNDKEIFTKDLGYKSERGLHFMGRMDDVINVSGLKVFPIEVEETMLRLEGVQEAIVYRGKHPVMGEIVKAKV
ISHIDPVQIREWCMQHLPSYKVPHEIESVTEIPKNKTGKVSRKLLEMGEVTT
>Mature_212_residues
MLHIMGSFPQGTFQFHKIMTSGAPLPEALFYKLKETTTYMMQQYGCSEAGCISICHDMKSHLDLGNPLPHASISIGSDEN
APEEIIVKMNDKEIFTKDLGYKSERGLHFMGRMDDVINVSGLKVFPIEVEETMLRLEGVQEAIVYRGKHPVMGEIVKAKV
ISHIDPVQIREWCMQHLPSYKVPHEIESVTEIPKNKTGKVSRKLLEMGEVTT

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI157311624, Length=183, Percent_Identity=35.5191256830601, Blast_Score=86, Evalue=2e-17,
Organism=Homo sapiens, GI157311622, Length=183, Percent_Identity=35.5191256830601, Blast_Score=86, Evalue=2e-17,
Organism=Homo sapiens, GI58082049, Length=225, Percent_Identity=32, Blast_Score=84, Evalue=7e-17,
Organism=Homo sapiens, GI115511026, Length=126, Percent_Identity=38.8888888888889, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI28416953, Length=135, Percent_Identity=32.5925925925926, Blast_Score=68, Evalue=6e-12,
Organism=Homo sapiens, GI38505220, Length=128, Percent_Identity=34.375, Blast_Score=67, Evalue=9e-12,
Organism=Homo sapiens, GI42544132, Length=143, Percent_Identity=30.7692307692308, Blast_Score=65, Evalue=4e-11,
Organism=Escherichia coli, GI1788107, Length=233, Percent_Identity=27.8969957081545, Blast_Score=67, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI25141359, Length=201, Percent_Identity=30.8457711442786, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6319699, Length=128, Percent_Identity=29.6875, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 23918; Mature: 23918

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
6.1 %Met     (Translated Protein)
8.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
6.1 %Met     (Mature Protein)
8.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHIMGSFPQGTFQFHKIMTSGAPLPEALFYKLKETTTYMMQQYGCSEAGCISICHDMKS
CEEECCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
HLDLGNPLPHASISIGSDENAPEEIIVKMNDKEIFTKDLGYKSERGLHFMGRMDDVINVS
HCCCCCCCCCCEEECCCCCCCCHHEEEEECCHHHHHHHCCCCCCCCEEHHHCCCCCEECC
GLKVFPIEVEETMLRLEGVQEAIVYRGKHPVMGEIVKAKVISHIDPVQIREWCMQHLPSY
CCEEEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCC
KVPHEIESVTEIPKNKTGKVSRKLLEMGEVTT
CCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLHIMGSFPQGTFQFHKIMTSGAPLPEALFYKLKETTTYMMQQYGCSEAGCISICHDMKS
CEEECCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
HLDLGNPLPHASISIGSDENAPEEIIVKMNDKEIFTKDLGYKSERGLHFMGRMDDVINVS
HCCCCCCCCCCEEECCCCCCCCHHEEEEECCHHHHHHHCCCCCCCCEEHHHCCCCCEECC
GLKVFPIEVEETMLRLEGVQEAIVYRGKHPVMGEIVKAKVISHIDPVQIREWCMQHLPSY
CCEEEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCC
KVPHEIESVTEIPKNKTGKVSRKLLEMGEVTT
CCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]