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The map label for this gene is asbF

Identifier: 227815193

GI number: 227815193

Start: 2367287

End: 2368129

Strand: Reverse

Name: asbF

Synonym: BAMEG_2604

Alternate gene names: 227815193

Gene position: 2368129-2367287 (Counterclockwise)

Preceding gene: 227815194

Following gene: 227815192

Centisome position: 45.28

GC content: 33.45

Gene sequence:

>843_bases
ATGAAATATTCACTATGTACCATTTCATTTCGCCATCAATTAATTTCATTTACTGATATTGTTCAATTTGCATATGAAAA
CGGTTTTGAAGGCATTGAATTATGGGGGACTCATGCACAAAATTTGTATATGCAGGAGTATGAAACGACAGAACGAGAAT
TGAATTGTTTAAAGGATAAAACCTTGGAAATTACGATGATAAGTGATTACTTAGATATATCATTATCGGCAGATTTTGAA
AAAACGATAGAGAAATGTGAACAACTTGCAATACTAGCTAATTGGTTTAAAACGAACAAAATTCGTACGTTTGCTGGACA
AAAAGGTAGTGCAGATTTTTCGCAACAAGAGAGACAAGAGTATGTGAATCGTATTCGCATGATTTGTGAATTATTTGCTC
AGCATAATATGTATGTACTTTTAGAAACACACCCAAATACGTTAACGGATACGTTGCCTTCTACTTTGGAATTATTAGGC
GAAGTAGATCATCCGAATTTGAAAATAAATCTTGATTTTCTTCATATATGGGAGTCTGGTGCAGATCCAGTTGACAGCTT
CCAGCAACTAAGACCGTGGATACAACATTACCATTTTAAGAATATATCTTCAGCAGATTATTTACATGTGTTCGAACCGA
ATAATGTATATGCAGCAGCGGGAAATCGTACTGGTATGGTTCCATTATTTGAAGGCATTGTAAATTATGATGAGATTATT
CAGGAAGTGAGAGATACTGATCATTTCGCTTCACTCGAATGGTTTGGACATAATGCGAAAGATATATTAAAAGCAGAAAT
GAAAGTACTAACAAATAGAAATTTAGAAGTAGTAACTTCTTAA

Upstream 100 bases:

>100_bases
TTAGAGAGACAGTTTTTATCATGGAAAGAAATGAAATCAAATGAGTCTGTCTTAGTATTTTAAAAACTTTACTGAATTTA
AGATAAGGGAGTTCAAACCT

Downstream 100 bases:

>100_bases
TATAAAATGATGAAAGAGTCTCTCATTGTAGAGACTCTTTTTTAAAAATATTAATAACCAAAAATTACCATGTATTTAAA
TTAAAATTTAACACAATTTA

Product: petrobactin biosynthesis protein AsbF

Products: NA

Alternate protein names: 3-DHS dehydratase; DHSase; Petrobactin biosynthesis protein AsbF

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MKYSLCTISFRHQLISFTDIVQFAYENGFEGIELWGTHAQNLYMQEYETTERELNCLKDKTLEITMISDYLDISLSADFE
KTIEKCEQLAILANWFKTNKIRTFAGQKGSADFSQQERQEYVNRIRMICELFAQHNMYVLLETHPNTLTDTLPSTLELLG
EVDHPNLKINLDFLHIWESGADPVDSFQQLRPWIQHYHFKNISSADYLHVFEPNNVYAAAGNRTGMVPLFEGIVNYDEII
QEVRDTDHFASLEWFGHNAKDILKAEMKVLTNRNLEVVTS

Sequences:

>Translated_280_residues
MKYSLCTISFRHQLISFTDIVQFAYENGFEGIELWGTHAQNLYMQEYETTERELNCLKDKTLEITMISDYLDISLSADFE
KTIEKCEQLAILANWFKTNKIRTFAGQKGSADFSQQERQEYVNRIRMICELFAQHNMYVLLETHPNTLTDTLPSTLELLG
EVDHPNLKINLDFLHIWESGADPVDSFQQLRPWIQHYHFKNISSADYLHVFEPNNVYAAAGNRTGMVPLFEGIVNYDEII
QEVRDTDHFASLEWFGHNAKDILKAEMKVLTNRNLEVVTS
>Mature_280_residues
MKYSLCTISFRHQLISFTDIVQFAYENGFEGIELWGTHAQNLYMQEYETTERELNCLKDKTLEITMISDYLDISLSADFE
KTIEKCEQLAILANWFKTNKIRTFAGQKGSADFSQQERQEYVNRIRMICELFAQHNMYVLLETHPNTLTDTLPSTLELLG
EVDHPNLKINLDFLHIWESGADPVDSFQQLRPWIQHYHFKNISSADYLHVFEPNNVYAAAGNRTGMVPLFEGIVNYDEII
QEVRDTDHFASLEWFGHNAKDILKAEMKVLTNRNLEVVTS

Specific function: Converts 3-dehydroshikimate to protocatechuate (3,4- dihydroxybenzoate), a biosynthetic unit required for the biosynthesis of petrobactin which is a virulence-associated siderophore produced by B.anthracis

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ASBF_BACAN (Q81RQ4)

Other databases:

- EMBL:   AE016879
- EMBL:   AE017334
- EMBL:   AE017225
- RefSeq:   NP_844392.1
- RefSeq:   YP_018630.1
- RefSeq:   YP_028107.1
- PDB:   3DX5
- PDBsum:   3DX5
- ProteinModelPortal:   Q81RQ4
- EnsemblBacteria:   EBBACT00000008979
- EnsemblBacteria:   EBBACT00000013524
- EnsemblBacteria:   EBBACT00000021258
- GeneID:   1085888
- GeneID:   2815037
- GeneID:   2848581
- GenomeReviews:   AE016879_GR
- GenomeReviews:   AE017225_GR
- GenomeReviews:   AE017334_GR
- KEGG:   ban:BA_1986
- KEGG:   bar:GBAA_1986
- KEGG:   bat:BAS1843
- TIGR:   BA_1986
- TIGR:   GBAA_1986
- GeneTree:   EBGT00050000002043
- HOGENOM:   HBG340798
- OMA:   DGIELWG
- ProtClustDB:   CLSK916442
- BioCyc:   BANT260799:BAS1843-MONOMER
- BioCyc:   BANT261594:GBAA1986-MONOMER
- BioCyc:   MetaCyc:MONOMER-14940
- InterPro:   IPR013022
- InterPro:   IPR012307
- Gene3D:   G3DSA:3.20.20.150

Pfam domain/function: PF01261 AP_endonuc_2; SSF51658 Xyl_isomerase-like_TIM-brl

EC number: =4.2.1.118

Molecular weight: Translated: 32589; Mature: 32589

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: ACT_SITE 144-144 BINDING 70-70 BINDING 102-102 BINDING 142-142 BINDING 172-172 BINDING 175-175 BINDING 217-217 BINDING 253-253

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYSLCTISFRHQLISFTDIVQFAYENGFEGIELWGTHAQNLYMQEYETTERELNCLKDK
CCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCC
TLEITMISDYLDISLSADFEKTIEKCEQLAILANWFKTNKIRTFAGQKGSADFSQQERQE
CEEEEEEECEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHH
YVNRIRMICELFAQHNMYVLLETHPNTLTDTLPSTLELLGEVDHPNLKINLDFLHIWESG
HHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCC
ADPVDSFQQLRPWIQHYHFKNISSADYLHVFEPNNVYAAAGNRTGMVPLFEGIVNYDEII
CCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEECCCCCCCHHHHHHHCCHHHHH
QEVRDTDHFASLEWFGHNAKDILKAEMKVLTNRNLEVVTS
HHHHCCCHHEEHHHCCCCHHHHHHHHHHHHHCCCEEEEEC
>Mature Secondary Structure
MKYSLCTISFRHQLISFTDIVQFAYENGFEGIELWGTHAQNLYMQEYETTERELNCLKDK
CCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCC
TLEITMISDYLDISLSADFEKTIEKCEQLAILANWFKTNKIRTFAGQKGSADFSQQERQE
CEEEEEEECEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHH
YVNRIRMICELFAQHNMYVLLETHPNTLTDTLPSTLELLGEVDHPNLKINLDFLHIWESG
HHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCC
ADPVDSFQQLRPWIQHYHFKNISSADYLHVFEPNNVYAAAGNRTGMVPLFEGIVNYDEII
CCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEECCCCCCCHHHHHHHCCHHHHH
QEVRDTDHFASLEWFGHNAKDILKAEMKVLTNRNLEVVTS
HHHHCCCHHEEHHHCCCCHHHHHHHHHHHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629