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The map label for this gene is thiF [H]

Identifier: 227815046

GI number: 227815046

Start: 2245419

End: 2246432

Strand: Reverse

Name: thiF [H]

Synonym: BAMEG_2457

Alternate gene names: 227815046

Gene position: 2246432-2245419 (Counterclockwise)

Preceding gene: 227815047

Following gene: 227815045

Centisome position: 42.95

GC content: 37.87

Gene sequence:

>1014_bases
ATGCAAGAGCGATATTCAAGACAAGTATTGTTTTCTGGAATAGGTGAAATGGGACAAAGGAAAATAAGGGAAAAGCATGT
GCTCTTAATTGGTGCGGGGGCGCTAGGAGCTGCAAATGCAGAAGCGCTCGTTCGGATGGGAATTGGGAAATTGACAATTG
CCGATCGTGATTATGTCGAATGGAGTAATTTACAACGGCAACAGTTATATACAGAAGAAGATGCAAAACAATGTAAACCA
AAAGCAATTGCAGCGGCAGAACATGTAAGAAAGATTAATTCTGAGGTGGAAATTGTACCAGTTGTAACGGATGTCACAAT
GAAAGAAATGGAAGAGTTAACGAAAGAAGTGGATCTCATATTAGATGCGACTGACAACTTTGATACGCGTCTACTTATAA
ATGATATTTCACAAAAAGAAAATATACCTTGGATATACGGTGGATGCATTGGAAGTTACGGTGTAACGTACACAATTCTT
CCAGGAAAAACACCATGTTTTCGCTGCTTAATGGATCATCCGATGGGCGGTGCAACATGTGATACAGCGGGAATTATTCA
GCCAGCTGTACAGATGGTTGTTGCTCACCAAGTGACAGAAGCAATGAAAATATTGGTGGATGACTTCGAGACGCTACGAG
GAACAATGTTATCATTTGATATTTGGAACAATCAATTTCTCTCATTAAAAGTAAATAAACAGAAAAAAAGTACATGTCCA
TCTTGCGGAAATACACGTACGTACCCAAGTTTAACATTTGAATCACAGGTGAAAACGGAGGTGCTATGCGGACGGAATAC
AGTTCAAATCCGTCCAGGAATAAAGAGAGTTCTAAATTTAAACGAAATTAAAAAAAGATTACAAAAAATTGTACATGTCC
AAAAAACGCCGTACTTACTATCATTTCTAATTGATGAATATCGTTTCGTTTTATTTACAGACGGTAGGGCATTTGTTCAT
GGGACAAATGATGTGAAAATAGGAAAACGATTGTACGCAAAATATATAGGATGA

Upstream 100 bases:

>100_bases
GAACAGATCGATATTCAGATGAAAGAACGGCGGAAAGTACAAATAAACGTCAAAAAGTTGAGATGTCTTACATTGGTGGA
TAGCGAAAGGAGGATTCCTT

Downstream 100 bases:

>100_bases
ACAGAAAGAAAGTTTCTCCTTATTAAAACGAGGTGAAAAAGAATGTTAGCAAAACGTATACCAATTCCAGTGGCGGAAGC
AGTTGCACGAGTAATGAAAT

Product: thiamine/molybdopterin biosynthesis MoeB-like protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MQERYSRQVLFSGIGEMGQRKIREKHVLLIGAGALGAANAEALVRMGIGKLTIADRDYVEWSNLQRQQLYTEEDAKQCKP
KAIAAAEHVRKINSEVEIVPVVTDVTMKEMEELTKEVDLILDATDNFDTRLLINDISQKENIPWIYGGCIGSYGVTYTIL
PGKTPCFRCLMDHPMGGATCDTAGIIQPAVQMVVAHQVTEAMKILVDDFETLRGTMLSFDIWNNQFLSLKVNKQKKSTCP
SCGNTRTYPSLTFESQVKTEVLCGRNTVQIRPGIKRVLNLNEIKKRLQKIVHVQKTPYLLSFLIDEYRFVLFTDGRAFVH
GTNDVKIGKRLYAKYIG

Sequences:

>Translated_337_residues
MQERYSRQVLFSGIGEMGQRKIREKHVLLIGAGALGAANAEALVRMGIGKLTIADRDYVEWSNLQRQQLYTEEDAKQCKP
KAIAAAEHVRKINSEVEIVPVVTDVTMKEMEELTKEVDLILDATDNFDTRLLINDISQKENIPWIYGGCIGSYGVTYTIL
PGKTPCFRCLMDHPMGGATCDTAGIIQPAVQMVVAHQVTEAMKILVDDFETLRGTMLSFDIWNNQFLSLKVNKQKKSTCP
SCGNTRTYPSLTFESQVKTEVLCGRNTVQIRPGIKRVLNLNEIKKRLQKIVHVQKTPYLLSFLIDEYRFVLFTDGRAFVH
GTNDVKIGKRLYAKYIG
>Mature_337_residues
MQERYSRQVLFSGIGEMGQRKIREKHVLLIGAGALGAANAEALVRMGIGKLTIADRDYVEWSNLQRQQLYTEEDAKQCKP
KAIAAAEHVRKINSEVEIVPVVTDVTMKEMEELTKEVDLILDATDNFDTRLLINDISQKENIPWIYGGCIGSYGVTYTIL
PGKTPCFRCLMDHPMGGATCDTAGIIQPAVQMVVAHQVTEAMKILVDDFETLRGTMLSFDIWNNQFLSLKVNKQKKSTCP
SCGNTRTYPSLTFESQVKTEVLCGRNTVQIRPGIKRVLNLNEIKKRLQKIVHVQKTPYLLSFLIDEYRFVLFTDGRAFVH
GTNDVKIGKRLYAKYIG

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein ThiS [H]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=267, Percent_Identity=30.3370786516854, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI38045948, Length=170, Percent_Identity=32.3529411764706, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI150417996, Length=183, Percent_Identity=29.5081967213115, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI1787048, Length=242, Percent_Identity=32.6446280991736, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI87082356, Length=249, Percent_Identity=34.9397590361446, Blast_Score=128, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI17540406, Length=307, Percent_Identity=30.9446254071661, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI193203301, Length=162, Percent_Identity=30.2469135802469, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6321903, Length=270, Percent_Identity=26.6666666666667, Blast_Score=107, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6320598, Length=163, Percent_Identity=35.5828220858896, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6325323, Length=155, Percent_Identity=32.9032258064516, Blast_Score=73, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6322639, Length=185, Percent_Identity=28.1081081081081, Blast_Score=67, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6325438, Length=153, Percent_Identity=30.718954248366, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24582879, Length=298, Percent_Identity=26.1744966442953, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI28573937, Length=179, Percent_Identity=27.3743016759777, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24641311, Length=163, Percent_Identity=33.7423312883436, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24660640, Length=152, Percent_Identity=31.5789473684211, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF [H]

EC number: NA

Molecular weight: Translated: 37998; Mature: 37998

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQERYSRQVLFSGIGEMGQRKIREKHVLLIGAGALGAANAEALVRMGIGKLTIADRDYVE
CCCHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCHHHHHHHCCCEEEECCCCCHH
WSNLQRQQLYTEEDAKQCKPKAIAAAEHVRKINSEVEIVPVVTDVTMKEMEELTKEVDLI
HHCCHHHHHHCHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEEECHHHHHHHHHHHHHHEE
LDATDNFDTRLLINDISQKENIPWIYGGCIGSYGVTYTILPGKTPCFRCLMDHPMGGATC
EECCCCCCCEEEEECHHHCCCCCEEEECCCCCCCCEEEEECCCCHHHHHHHHCCCCCCCC
DTAGIIQPAVQMVVAHQVTEAMKILVDDFETLRGTMLSFDIWNNQFLSLKVNKQKKSTCP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEEEEEEECCCCCCCCC
SCGNTRTYPSLTFESQVKTEVLCGRNTVQIRPGIKRVLNLNEIKKRLQKIVHVQKTPYLL
CCCCCCCCCCEEEHHHHHHHHHCCCCEEEECCHHHHHCCHHHHHHHHHHHHHHCCCHHHH
SFLIDEYRFVLFTDGRAFVHGTNDVKIGKRLYAKYIG
HHHHHCCEEEEEECCEEEEECCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MQERYSRQVLFSGIGEMGQRKIREKHVLLIGAGALGAANAEALVRMGIGKLTIADRDYVE
CCCHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCHHHHHHHCCCEEEECCCCCHH
WSNLQRQQLYTEEDAKQCKPKAIAAAEHVRKINSEVEIVPVVTDVTMKEMEELTKEVDLI
HHCCHHHHHHCHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEEECHHHHHHHHHHHHHHEE
LDATDNFDTRLLINDISQKENIPWIYGGCIGSYGVTYTILPGKTPCFRCLMDHPMGGATC
EECCCCCCCEEEEECHHHCCCCCEEEECCCCCCCCEEEEECCCCHHHHHHHHCCCCCCCC
DTAGIIQPAVQMVVAHQVTEAMKILVDDFETLRGTMLSFDIWNNQFLSLKVNKQKKSTCP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEEEEEEECCCCCCCCC
SCGNTRTYPSLTFESQVKTEVLCGRNTVQIRPGIKRVLNLNEIKKRLQKIVHVQKTPYLL
CCCCCCCCCCEEEHHHHHHHHHCCCCEEEECCHHHHHCCHHHHHHHHHHHHHHCCCHHHH
SFLIDEYRFVLFTDGRAFVHGTNDVKIGKRLYAKYIG
HHHHHCCEEEEEECCEEEEECCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]