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The map label for this gene is azoR2 [H]

Identifier: 227814944

GI number: 227814944

Start: 2146774

End: 2147409

Strand: Reverse

Name: azoR2 [H]

Synonym: BAMEG_2355

Alternate gene names: 227814944

Gene position: 2147409-2146774 (Counterclockwise)

Preceding gene: 227814945

Following gene: 227814943

Centisome position: 41.06

GC content: 37.26

Gene sequence:

>636_bases
ATGACAAAAGTACTATTCATTACAGCAAATCCAAATTCAGCAGAAGGTTCATTCGGAATGGCAGTAGGGGAAGCTTTCAT
CGAAGCTTATAAAAATGAACATCCACAAGACGAAGTTGTAACAATTGATTTATTCAACACTACAGTACCAGCAATCGATG
CAGATGTATTCGCAGCTTGGGGTAAATTTGCAGCAGGTGAAGGATTTGAAGCTTTAACTGAAGCACAACAACAAAAAGTT
GCAGCAATGAACACAAACTTAGAAACATTTATGCATGCAGATCGTTATGTATTCGTCACTCCAATGTGGAACTTTAGCTA
TCCATCAGTAGTAAAAGCATACTTAGATAACTTAGCAATCGCAGGTAAAACATTCAAATATACTGAAAATGGACCAGTTG
GCTTATTAGAAGGCAAAAAAGCACTTCACATTCAAGCAACAGGTGGCGTTTATTCTGAAGGACCATACGCAGCTGTAGAC
TTTGGCCGCAATCACTTAAAAACAGTATTAGGATTCATCGGTGTAAATGAAACTGAATATATTGCAGTTGAAGGCATGAA
TGCAAATCCTGAAAAAGCACAAGAAATTAAAGAAGCAGCAATTGCTAATGCTCGTGAATTAGCAAAACGTTTCTAA

Upstream 100 bases:

>100_bases
AAACGTAACTTTTTATATTATACTTACATAAGGTAAGTTATTTACTTTCAAATTATAAATCTCAAATTAAAGATAAAATG
TAGAAAAGGGAGAGAATACA

Downstream 100 bases:

>100_bases
TATATCATACTTAAAAATGTCCAAACACTTGCTGTTTGGACATTTTTTCTTCTTTTTCCTTCGTTTGTCTAGTATAATGA
AGGTCGGAATGATAATAGGT

Product: azoreductase

Products: NAD+; NADP+; reduced acceptor

Alternate protein names: Azo-dye reductase 2; FMN-dependent NADH-azo compound oxidoreductase 2 [H]

Number of amino acids: Translated: 211; Mature: 210

Protein sequence:

>211_residues
MTKVLFITANPNSAEGSFGMAVGEAFIEAYKNEHPQDEVVTIDLFNTTVPAIDADVFAAWGKFAAGEGFEALTEAQQQKV
AAMNTNLETFMHADRYVFVTPMWNFSYPSVVKAYLDNLAIAGKTFKYTENGPVGLLEGKKALHIQATGGVYSEGPYAAVD
FGRNHLKTVLGFIGVNETEYIAVEGMNANPEKAQEIKEAAIANARELAKRF

Sequences:

>Translated_211_residues
MTKVLFITANPNSAEGSFGMAVGEAFIEAYKNEHPQDEVVTIDLFNTTVPAIDADVFAAWGKFAAGEGFEALTEAQQQKV
AAMNTNLETFMHADRYVFVTPMWNFSYPSVVKAYLDNLAIAGKTFKYTENGPVGLLEGKKALHIQATGGVYSEGPYAAVD
FGRNHLKTVLGFIGVNETEYIAVEGMNANPEKAQEIKEAAIANARELAKRF
>Mature_210_residues
TKVLFITANPNSAEGSFGMAVGEAFIEAYKNEHPQDEVVTIDLFNTTVPAIDADVFAAWGKFAAGEGFEALTEAQQQKVA
AMNTNLETFMHADRYVFVTPMWNFSYPSVVKAYLDNLAIAGKTFKYTENGPVGLLEGKKALHIQATGGVYSEGPYAAVDF
GRNHLKTVLGFIGVNETEYIAVEGMNANPEKAQEIKEAAIANARELAKRF

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADH, but not NADPH, as an electron donor for its activity [H]

COG id: COG1182

COG function: function code I; Acyl carrier protein phosphodiesterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the azoreductase type 1 family [H]

Homologues:

Organism=Escherichia coli, GI1787680, Length=204, Percent_Identity=34.8039215686275, Blast_Score=114, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003680
- InterPro:   IPR023048 [H]

Pfam domain/function: PF02525 Flavodoxin_2 [H]

EC number: 1.6.99.2

Molecular weight: Translated: 22984; Mature: 22853

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKVLFITANPNSAEGSFGMAVGEAFIEAYKNEHPQDEVVTIDLFNTTVPAIDADVFAAW
CCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHHHHH
GKFAAGEGFEALTEAQQQKVAAMNTNLETFMHADRYVFVTPMWNFSYPSVVKAYLDNLAI
CCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHEE
AGKTFKYTENGPVGLLEGKKALHIQATGGVYSEGPYAAVDFGRNHLKTVLGFIGVNETEY
CCCEEEECCCCCEEEEECCEEEEEEECCCCCCCCCEEEECCCHHHHHHHHHHHCCCCCEE
IAVEGMNANPEKAQEIKEAAIANARELAKRF
EEEECCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TKVLFITANPNSAEGSFGMAVGEAFIEAYKNEHPQDEVVTIDLFNTTVPAIDADVFAAW
CEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHHHHH
GKFAAGEGFEALTEAQQQKVAAMNTNLETFMHADRYVFVTPMWNFSYPSVVKAYLDNLAI
CCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHEE
AGKTFKYTENGPVGLLEGKKALHIQATGGVYSEGPYAAVDFGRNHLKTVLGFIGVNETEY
CCCEEEECCCCCEEEEECCEEEEEEECCCCCCCCCEEEECCCHHHHHHHHHHHCCCCCEE
IAVEGMNANPEKAQEIKEAAIANARELAKRF
EEEECCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; NADPH; H+; acceptor

Specific reaction: NAD(P)H + H+ + acceptor = NAD(P)+ + reduced acceptor

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721630 [H]