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The map label for this gene is asnO3 [H]

Identifier: 227814934

GI number: 227814934

Start: 2137746

End: 2139647

Strand: Reverse

Name: asnO3 [H]

Synonym: BAMEG_2345

Alternate gene names: 227814934

Gene position: 2139647-2137746 (Counterclockwise)

Preceding gene: 227814936

Following gene: 227814933

Centisome position: 40.91

GC content: 36.59

Gene sequence:

>1902_bases
ATGTGTGGTTTTGTAGGATGTTTATGTGAAAACCCTAGAGAGTTTTCAGAAACAGAAAAACATCAATTTGAAAATATGAA
CACGATGATTTTCCACCGTGGTCCAGATGACGAAGGATATTTTCGTGATGAACATGTACAATTTGGCTTCCGCCGTTTAA
GTATCATTGACTTAGAGGCAGGACATCAGCCGCTAACTTATGAAAATGATCGATATGTAATTATTTTTAATGGTGAAATT
TACAACTATGTAGAATTACGTGAAATGTTACTTGAAAAAGGTGCAACGTTTGCAACGCAATCTGATACAGAAGTTATCAT
TGCATTGTATGCACATATGAAAGAAAAATGTGTAGACTACCTTCGTGGTATGTTTGCATTTATGATTTGGGATCGTGAAG
AGAAGAAACTTTTCGGAGCACGTGATCACTTCGGTATTAAACCTTTATACATCGCACAACAAGGTGATACTACATTCTTC
GCATCTGAGAAGAAAAGTATTATGCATGTGATGGAAGATAAAGGCGTTAATCCAACGTCACTACAACATTACTTTACGTA
TCAATATGGTCCAGAGCCAGAAACATTAACAATTGATGTTAATAAAATCGAGCCTGGTCATTATTTCGTAAAAGAAATCG
GTAAAGAGATGGAAATCCATCGCTACTGGAAACCTTATTTCAATGCTTCAAGTGCAACGAAAGAGGAACATATCCAAGCG
ATTCGTGATGTGTTATATGATTCAGTAAAAGTGCATATGCGCAGTGATGTACCAGTAGGTTCATTCTTATCTGGTGGTAT
CGATTCATCTATTATCGCTTCTATTGCAAGAGAAATGAATCCAAATCTTTTAACATTCTCTGTTGGTTTTGAGCAACGTG
GTTTTAGTGAAGTTGATGTTGCGAAAGAAACTGCTGAGAAATTAGGCGTTAAAAACCATAACGTATTCATTTCAGCGAAA
GAGTTTATGGATGAGTTCCCAAAAATCATTTGGCATATGGATGATCCTTTAGCAGATCCGGCAGCTGTACCATTGTACTT
CGTTGCAAAAGAAGCACGTAAACATGTAACAGTTGTTCTTTCAGGTGAAGGTGCAGACGAGCTATTTGGTGGTTATAACA
TTTACCGTGAGCCAAACTCACTGAAAATGTTCTCTTACATTCCTAGCCCAGGTAAGAGCGTTCTAAAAGCATTAAGTGGT
GCTCTTAAAGAAGGCTTTAAAGGAAAGAGCTTCCTAGAGCGTGGATGTACGCCAATTGAAGAGCGTTACTATGGAAACGC
GAAAATCTTCCGTGAAGAAGAGAAAGCTGAATTAATGAAGTATTACAATGAAAGTGTTAACTATATGGATATCACGAAAC
CATTGTATAACGAAATTAAAGATTATGATGATGTAAGTAAAATGCAGTACATTGACATGTTCACATGGTTACGCGGTGAC
ATTTTATTAAAAGCTGATAAAATGACAATGGCGAATTCATTAGAACTTCGTGTACCGTTCTTAGATAAAGAAGTATTCGA
TGTTGCATCTAAAATTCCAACTGAATTTAAGATTGCTAACGGAACTACGAAAGCTATTTTACGTGAAGCAGCACGCGGAA
TCGTTCCAGATCACGTATTAGATCGTAAAAAACTTGGATTCCCAGTACCAATTCGTCACTGGCTAAAAGACGAAATGCAT
GATTGGGCTATTAATATTATAAACGAAAGTAAGACAGAGCATTTAATCGACAAACAGTATGTATTAAACTTACTGGAAGC
ACATTGTGCAGATAAAGGCGATTATAGCCGTAAAATTTGGACTGTACTTGCATTTATGGTATGGCATCAAATTTATGTTG
AGCATAAATACGATACGAATAAGTTCCACGAAGAAACGAAACGTGCGTATAGCTTAGTTTAA

Upstream 100 bases:

>100_bases
TTATAGTTTTTGGTCGCCATATTCAGTTGATTATGCTAGTATAAATAGGGTTGATATAGTAGTATAGAGACAGAATGAAA
ACTGAAAGAGGGTAAGAAGT

Downstream 100 bases:

>100_bases
TTAGAAAAACCTTAAGATCGCATACGGTCTTAAGGTTTTTTTCATAAATAGGACGTATTATTGATACAATAATAGTAAAA
GGATGGACAACATATGATTA

Product: asparagine synthetase, glutamine-hydrolyzing

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 633; Mature: 633

Protein sequence:

>633_residues
MCGFVGCLCENPREFSETEKHQFENMNTMIFHRGPDDEGYFRDEHVQFGFRRLSIIDLEAGHQPLTYENDRYVIIFNGEI
YNYVELREMLLEKGATFATQSDTEVIIALYAHMKEKCVDYLRGMFAFMIWDREEKKLFGARDHFGIKPLYIAQQGDTTFF
ASEKKSIMHVMEDKGVNPTSLQHYFTYQYGPEPETLTIDVNKIEPGHYFVKEIGKEMEIHRYWKPYFNASSATKEEHIQA
IRDVLYDSVKVHMRSDVPVGSFLSGGIDSSIIASIAREMNPNLLTFSVGFEQRGFSEVDVAKETAEKLGVKNHNVFISAK
EFMDEFPKIIWHMDDPLADPAAVPLYFVAKEARKHVTVVLSGEGADELFGGYNIYREPNSLKMFSYIPSPGKSVLKALSG
ALKEGFKGKSFLERGCTPIEERYYGNAKIFREEEKAELMKYYNESVNYMDITKPLYNEIKDYDDVSKMQYIDMFTWLRGD
ILLKADKMTMANSLELRVPFLDKEVFDVASKIPTEFKIANGTTKAILREAARGIVPDHVLDRKKLGFPVPIRHWLKDEMH
DWAINIINESKTEHLIDKQYVLNLLEAHCADKGDYSRKIWTVLAFMVWHQIYVEHKYDTNKFHEETKRAYSLV

Sequences:

>Translated_633_residues
MCGFVGCLCENPREFSETEKHQFENMNTMIFHRGPDDEGYFRDEHVQFGFRRLSIIDLEAGHQPLTYENDRYVIIFNGEI
YNYVELREMLLEKGATFATQSDTEVIIALYAHMKEKCVDYLRGMFAFMIWDREEKKLFGARDHFGIKPLYIAQQGDTTFF
ASEKKSIMHVMEDKGVNPTSLQHYFTYQYGPEPETLTIDVNKIEPGHYFVKEIGKEMEIHRYWKPYFNASSATKEEHIQA
IRDVLYDSVKVHMRSDVPVGSFLSGGIDSSIIASIAREMNPNLLTFSVGFEQRGFSEVDVAKETAEKLGVKNHNVFISAK
EFMDEFPKIIWHMDDPLADPAAVPLYFVAKEARKHVTVVLSGEGADELFGGYNIYREPNSLKMFSYIPSPGKSVLKALSG
ALKEGFKGKSFLERGCTPIEERYYGNAKIFREEEKAELMKYYNESVNYMDITKPLYNEIKDYDDVSKMQYIDMFTWLRGD
ILLKADKMTMANSLELRVPFLDKEVFDVASKIPTEFKIANGTTKAILREAARGIVPDHVLDRKKLGFPVPIRHWLKDEMH
DWAINIINESKTEHLIDKQYVLNLLEAHCADKGDYSRKIWTVLAFMVWHQIYVEHKYDTNKFHEETKRAYSLV
>Mature_633_residues
MCGFVGCLCENPREFSETEKHQFENMNTMIFHRGPDDEGYFRDEHVQFGFRRLSIIDLEAGHQPLTYENDRYVIIFNGEI
YNYVELREMLLEKGATFATQSDTEVIIALYAHMKEKCVDYLRGMFAFMIWDREEKKLFGARDHFGIKPLYIAQQGDTTFF
ASEKKSIMHVMEDKGVNPTSLQHYFTYQYGPEPETLTIDVNKIEPGHYFVKEIGKEMEIHRYWKPYFNASSATKEEHIQA
IRDVLYDSVKVHMRSDVPVGSFLSGGIDSSIIASIAREMNPNLLTFSVGFEQRGFSEVDVAKETAEKLGVKNHNVFISAK
EFMDEFPKIIWHMDDPLADPAAVPLYFVAKEARKHVTVVLSGEGADELFGGYNIYREPNSLKMFSYIPSPGKSVLKALSG
ALKEGFKGKSFLERGCTPIEERYYGNAKIFREEEKAELMKYYNESVNYMDITKPLYNEIKDYDDVSKMQYIDMFTWLRGD
ILLKADKMTMANSLELRVPFLDKEVFDVASKIPTEFKIANGTTKAILREAARGIVPDHVLDRKKLGFPVPIRHWLKDEMH
DWAINIINESKTEHLIDKQYVLNLLEAHCADKGDYSRKIWTVLAFMVWHQIYVEHKYDTNKFHEETKRAYSLV

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=392, Percent_Identity=28.3163265306122, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI168229252, Length=392, Percent_Identity=28.3163265306122, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI168229250, Length=392, Percent_Identity=28.3163265306122, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI296010848, Length=392, Percent_Identity=28.3163265306122, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI296010852, Length=319, Percent_Identity=27.2727272727273, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI296010850, Length=319, Percent_Identity=27.2727272727273, Blast_Score=96, Evalue=1e-19,
Organism=Escherichia coli, GI1786889, Length=403, Percent_Identity=31.2655086848635, Blast_Score=182, Evalue=8e-47,
Organism=Caenorhabditis elegans, GI71993933, Length=389, Percent_Identity=33.1619537275064, Blast_Score=165, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI25147557, Length=389, Percent_Identity=33.1619537275064, Blast_Score=165, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI17560178, Length=377, Percent_Identity=30.2387267904509, Blast_Score=120, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI25147560, Length=332, Percent_Identity=30.7228915662651, Blast_Score=117, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6321563, Length=428, Percent_Identity=28.7383177570093, Blast_Score=158, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6325403, Length=432, Percent_Identity=28.2407407407407, Blast_Score=153, Evalue=6e-38,
Organism=Drosophila melanogaster, GI45553209, Length=404, Percent_Identity=30.1980198019802, Blast_Score=169, Evalue=4e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 73413; Mature: 73413

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGFVGCLCENPREFSETEKHQFENMNTMIFHRGPDDEGYFRDEHVQFGFRRLSIIDLEA
CCCCHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHCCEEEEEEEECC
GHQPLTYENDRYVIIFNGEIYNYVELREMLLEKGATFATQSDTEVIIALYAHMKEKCVDY
CCCCCEECCCEEEEEECCCCHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHHHH
LRGMFAFMIWDREEKKLFGARDHFGIKPLYIAQQGDTTFFASEKKSIMHVMEDKGVNPTS
HHHHHHHHHCCCHHHHHCCCCCCCCCCEEEEEECCCCEEECCHHHHHHHHHHHCCCCCCC
LQHYFTYQYGPEPETLTIDVNKIEPGHYFVKEIGKEMEIHRYWKPYFNASSATKEEHIQA
EEEEEEEEECCCCCEEEEEEEECCCCHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHH
IRDVLYDSVKVHMRSDVPVGSFLSGGIDSSIIASIAREMNPNLLTFSVGFEQRGFSEVDV
HHHHHHHHHHEEEECCCCHHHHHHCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHH
AKETAEKLGVKNHNVFISAKEFMDEFPKIIWHMDDPLADPAAVPLYFVAKEARKHVTVVL
HHHHHHHCCCCCCEEEEEHHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHCCCEEEEEE
SGEGADELFGGYNIYREPNSLKMFSYIPSPGKSVLKALSGALKEGFKGKSFLERGCTPIE
ECCCHHHHHCCCEEEECCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHCCCCHH
ERYYGNAKIFREEEKAELMKYYNESVNYMDITKPLYNEIKDYDDVSKMQYIDMFTWLRGD
HHHCCCHHEECCHHHHHHHHHHHCCCCEEECCHHHHHHHHCCHHHHHHHHHHHHHHHCCC
ILLKADKMTMANSLELRVPFLDKEVFDVASKIPTEFKIANGTTKAILREAARGIVPDHVL
EEEEECCEECCCCCEEEECCCCHHHHHHHHHCCCCEEECCCHHHHHHHHHHHCCCCHHHH
DRKKLGFPVPIRHWLKDEMHDWAINIINESKTEHLIDKQYVLNLLEAHCADKGDYSRKIW
HHHHCCCCCCHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
TVLAFMVWHQIYVEHKYDTNKFHEETKRAYSLV
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MCGFVGCLCENPREFSETEKHQFENMNTMIFHRGPDDEGYFRDEHVQFGFRRLSIIDLEA
CCCCHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHCCEEEEEEEECC
GHQPLTYENDRYVIIFNGEIYNYVELREMLLEKGATFATQSDTEVIIALYAHMKEKCVDY
CCCCCEECCCEEEEEECCCCHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHHHH
LRGMFAFMIWDREEKKLFGARDHFGIKPLYIAQQGDTTFFASEKKSIMHVMEDKGVNPTS
HHHHHHHHHCCCHHHHHCCCCCCCCCCEEEEEECCCCEEECCHHHHHHHHHHHCCCCCCC
LQHYFTYQYGPEPETLTIDVNKIEPGHYFVKEIGKEMEIHRYWKPYFNASSATKEEHIQA
EEEEEEEEECCCCCEEEEEEEECCCCHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHH
IRDVLYDSVKVHMRSDVPVGSFLSGGIDSSIIASIAREMNPNLLTFSVGFEQRGFSEVDV
HHHHHHHHHHEEEECCCCHHHHHHCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHH
AKETAEKLGVKNHNVFISAKEFMDEFPKIIWHMDDPLADPAAVPLYFVAKEARKHVTVVL
HHHHHHHCCCCCCEEEEEHHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHCCCEEEEEE
SGEGADELFGGYNIYREPNSLKMFSYIPSPGKSVLKALSGALKEGFKGKSFLERGCTPIE
ECCCHHHHHCCCEEEECCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHCCCCHH
ERYYGNAKIFREEEKAELMKYYNESVNYMDITKPLYNEIKDYDDVSKMQYIDMFTWLRGD
HHHCCCHHEECCHHHHHHHHHHHCCCCEEECCHHHHHHHHCCHHHHHHHHHHHHHHHCCC
ILLKADKMTMANSLELRVPFLDKEVFDVASKIPTEFKIANGTTKAILREAARGIVPDHVL
EEEEECCEECCCCCEEEECCCCHHHHHHHHHCCCCEEECCCHHHHHHHHHHHCCCCHHHH
DRKKLGFPVPIRHWLKDEMHDWAINIINESKTEHLIDKQYVLNLLEAHCADKGDYSRKIW
HHHHCCCCCCHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
TVLAFMVWHQIYVEHKYDTNKFHEETKRAYSLV
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]