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The map label for this gene is mrdA [H]

Identifier: 227814689

GI number: 227814689

Start: 1913225

End: 1914991

Strand: Direct

Name: mrdA [H]

Synonym: BAMEG_2099

Alternate gene names: 227814689

Gene position: 1913225-1914991 (Clockwise)

Preceding gene: 227814684

Following gene: 227814693

Centisome position: 36.58

GC content: 32.65

Gene sequence:

>1767_bases
ATGTTACATACAAAAATAAAAAAATGGGGATTTTTTGCGGTTCTTTCTATTGTAGGTTTGATTTTACTAATTTTATTTAA
AATCAACTTCATTTCAATTACTATCGCAACTTCTTCAGCCGAAAGAGGAAAAATCTTTGATCAAAATGGTGTAATACTAG
CTACAAATAAGAAAGTTAAGTCTCTATATTGTACTTCTAATGATGAAAAGTTATCGAAACAAGATACATCAAACACATTA
GCTTTTTTCGAGCAATTTTCAAGTAAGTTTCAACATGATATTTCTTCAGAGAGCATAAAATCTCAATTACAACAATCTTG
TAAAAAACAGACTATGCATGATATACCGTTATACTCTGATATAGCTGAGAATGAACTTGCATTCATAGACAAAAACAAAC
CGAATAATGTAATAATTAAAGATGAATGGATTCGATATTATCCTAAACATGAAATTGGTTCACAGGTAATTGGGTATGTA
GAAAATAACCTTAATTCAAAGCATATGCCGGTTGGAAAAAGTGGGATTGAGCTGCAATATGAAGATGATTTAAAAGGAAA
ACCGGGTAAAACTCTTATTTTTAAAATGAATAATAAAGAGTTCTTATGGAACATTCAAAAAGTACAAAAAGGTAAGGATA
TCAGGCTATCTTTAGATTCTAAATTGCAACAAAAATCAGAGGAAGCATTACGATCGCAAATTAAGAAATTACCTGATGCT
AAAGCTGGTTATGCTGTAGTGAGTGATGCAAAAACTGGCGCAATTTTAACTATGGCCAACTCAGCAGTTTTTGATCCAAA
TATATTACATACTCATGTATCAACTAAAATAGATAACTTAAAATCACTTTCGCAAAATAAAGCAATTCAAAAATTAAAGT
ATGGTGAATCTTATATAAATATGGCCTCTACTATAAAACCACTTACTATTTTAATTGGATTAAACGAAAAACTTTTTCAA
CCTGAAGATACTTATTTAGATAAAGGTACTTTTGAGTATGATAATCAGAATAACATTACAAATGCACCTGGAACGCCTAC
AGGTGAGATTACACCGAGGCAAGCTATCATTAATTCGTCTAACACATTTATGACAGCAAAAGTAGCTCTTCCATTATTTA
ACCAACATAATGGCAATGTAGAAAAAGTAGCACATATATGGACAGATTATTTAATGCAATTCGGCCTACGTTCTAAAACC
GGGATTGATTTACCCTTTGAAGAAGACGGACAATATGAATTCCATCCTTCCAATAAGTTTGAAAATGGAATCTCCGCTTT
ATTAAATGCTTCGTGGGGCGGAAACGAAGTGCATACTCCTCTTCAACTCGCTCAATATGCAGCAACTTTGGCAAGGAAAG
GTGATAAATATAAACCTCAAATGACAAGTGCTATTATTGGTCAAAATGGTAAGGAAACAAAAAAGTTTAAACCAATTTTA
GAAAGTTCAAATCGTTATCCGACGAAATTTTGGAGTGTCGTGCAAGGTGGAATGAGTCAAAATATAGAGGAGATTAAAAA
GTTGCCCTTTGACGTCGCTGGTAAAACAGGTATTACGGGTTTTCCGGATGAGCAAGCAAGAATGATAAACCATTCTCTAT
TCATTGCATATGCTCCTACCGAAGATCCACAGATTGCCGTTTCTGTCGTTATTCCTGGAAGTAATTCAGAAAAAAACATT
GCTGCTCTCGTTACATCAGAAATTTTAGAGTCCTGGAATACTCTTCAAAAAGAGAATAAAAACAAAAAGGAAGGTTCATT
AAAGTGA

Upstream 100 bases:

>100_bases
CTGTAGTACTTAATAGACAATCTAAATAATAAAGTTGTTCCACATTTACAAAAAAAATAATTAACTATAAGATGAAGTAG
GCTACAAAAGGGAGGATTTT

Downstream 100 bases:

>100_bases
ACCTTCCTTTTTTACCAAATAGAATCTATATTTATTATTTTCCATATACCTTCATTATTCTTTTCAAACATAAATGAGTA
TTTAATGTCAGTTAAAACAT

Product: penicillin-binding protein

Products: NA

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 588; Mature: 588

Protein sequence:

>588_residues
MLHTKIKKWGFFAVLSIVGLILLILFKINFISITIATSSAERGKIFDQNGVILATNKKVKSLYCTSNDEKLSKQDTSNTL
AFFEQFSSKFQHDISSESIKSQLQQSCKKQTMHDIPLYSDIAENELAFIDKNKPNNVIIKDEWIRYYPKHEIGSQVIGYV
ENNLNSKHMPVGKSGIELQYEDDLKGKPGKTLIFKMNNKEFLWNIQKVQKGKDIRLSLDSKLQQKSEEALRSQIKKLPDA
KAGYAVVSDAKTGAILTMANSAVFDPNILHTHVSTKIDNLKSLSQNKAIQKLKYGESYINMASTIKPLTILIGLNEKLFQ
PEDTYLDKGTFEYDNQNNITNAPGTPTGEITPRQAIINSSNTFMTAKVALPLFNQHNGNVEKVAHIWTDYLMQFGLRSKT
GIDLPFEEDGQYEFHPSNKFENGISALLNASWGGNEVHTPLQLAQYAATLARKGDKYKPQMTSAIIGQNGKETKKFKPIL
ESSNRYPTKFWSVVQGGMSQNIEEIKKLPFDVAGKTGITGFPDEQARMINHSLFIAYAPTEDPQIAVSVVIPGSNSEKNI
AALVTSEILESWNTLQKENKNKKEGSLK

Sequences:

>Translated_588_residues
MLHTKIKKWGFFAVLSIVGLILLILFKINFISITIATSSAERGKIFDQNGVILATNKKVKSLYCTSNDEKLSKQDTSNTL
AFFEQFSSKFQHDISSESIKSQLQQSCKKQTMHDIPLYSDIAENELAFIDKNKPNNVIIKDEWIRYYPKHEIGSQVIGYV
ENNLNSKHMPVGKSGIELQYEDDLKGKPGKTLIFKMNNKEFLWNIQKVQKGKDIRLSLDSKLQQKSEEALRSQIKKLPDA
KAGYAVVSDAKTGAILTMANSAVFDPNILHTHVSTKIDNLKSLSQNKAIQKLKYGESYINMASTIKPLTILIGLNEKLFQ
PEDTYLDKGTFEYDNQNNITNAPGTPTGEITPRQAIINSSNTFMTAKVALPLFNQHNGNVEKVAHIWTDYLMQFGLRSKT
GIDLPFEEDGQYEFHPSNKFENGISALLNASWGGNEVHTPLQLAQYAATLARKGDKYKPQMTSAIIGQNGKETKKFKPIL
ESSNRYPTKFWSVVQGGMSQNIEEIKKLPFDVAGKTGITGFPDEQARMINHSLFIAYAPTEDPQIAVSVVIPGSNSEKNI
AALVTSEILESWNTLQKENKNKKEGSLK
>Mature_588_residues
MLHTKIKKWGFFAVLSIVGLILLILFKINFISITIATSSAERGKIFDQNGVILATNKKVKSLYCTSNDEKLSKQDTSNTL
AFFEQFSSKFQHDISSESIKSQLQQSCKKQTMHDIPLYSDIAENELAFIDKNKPNNVIIKDEWIRYYPKHEIGSQVIGYV
ENNLNSKHMPVGKSGIELQYEDDLKGKPGKTLIFKMNNKEFLWNIQKVQKGKDIRLSLDSKLQQKSEEALRSQIKKLPDA
KAGYAVVSDAKTGAILTMANSAVFDPNILHTHVSTKIDNLKSLSQNKAIQKLKYGESYINMASTIKPLTILIGLNEKLFQ
PEDTYLDKGTFEYDNQNNITNAPGTPTGEITPRQAIINSSNTFMTAKVALPLFNQHNGNVEKVAHIWTDYLMQFGLRSKT
GIDLPFEEDGQYEFHPSNKFENGISALLNASWGGNEVHTPLQLAQYAATLARKGDKYKPQMTSAIIGQNGKETKKFKPIL
ESSNRYPTKFWSVVQGGMSQNIEEIKKLPFDVAGKTGITGFPDEQARMINHSLFIAYAPTEDPQIAVSVVIPGSNSEKNI
AALVTSEILESWNTLQKENKNKKEGSLK

Specific function: Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross- linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=624, Percent_Identity=20.8333333333333, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1786272, Length=560, Percent_Identity=23.9285714285714, Blast_Score=91, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460
- InterPro:   IPR017790 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 65872; Mature: 65872

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHTKIKKWGFFAVLSIVGLILLILFKINFISITIATSSAERGKIFDQNGVILATNKKVK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCCCEECCCCEEEEECCCEE
SLYCTSNDEKLSKQDTSNTLAFFEQFSSKFQHDISSESIKSQLQQSCKKQTMHDIPLYSD
EEEECCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHH
IAENELAFIDKNKPNNVIIKDEWIRYYPKHEIGSQVIGYVENNLNSKHMPVGKSGIELQY
HCCCCEEEEECCCCCEEEEECHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEE
EDDLKGKPGKTLIFKMNNKEFLWNIQKVQKGKDIRLSLDSKLQQKSEEALRSQIKKLPDA
CCCCCCCCCCEEEEEECCCCEEEEHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCC
KAGYAVVSDAKTGAILTMANSAVFDPNILHTHVSTKIDNLKSLSQNKAIQKLKYGESYIN
CCCEEEEECCCCCEEEEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
MASTIKPLTILIGLNEKLFQPEDTYLDKGTFEYDNQNNITNAPGTPTGEITPRQAIINSS
HHHCCCEEEEEEECCHHHCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCHHHHCCCC
NTFMTAKVALPLFNQHNGNVEKVAHIWTDYLMQFGLRSKTGIDLPFEEDGQYEFHPSNKF
CCEEEEEEEEEHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCHH
ENGISALLNASWGGNEVHTPLQLAQYAATLARKGDKYKPQMTSAIIGQNGKETKKFKPIL
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCHHHHHHHHH
ESSNRYPTKFWSVVQGGMSQNIEEIKKLPFDVAGKTGITGFPDEQARMINHSLFIAYAPT
HCCCCCCHHHHHHHHCHHHCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCEEEEEECCC
EDPQIAVSVVIPGSNSEKNIAALVTSEILESWNTLQKENKNKKEGSLK
CCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MLHTKIKKWGFFAVLSIVGLILLILFKINFISITIATSSAERGKIFDQNGVILATNKKVK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCCCEECCCCEEEEECCCEE
SLYCTSNDEKLSKQDTSNTLAFFEQFSSKFQHDISSESIKSQLQQSCKKQTMHDIPLYSD
EEEECCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHH
IAENELAFIDKNKPNNVIIKDEWIRYYPKHEIGSQVIGYVENNLNSKHMPVGKSGIELQY
HCCCCEEEEECCCCCEEEEECHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEE
EDDLKGKPGKTLIFKMNNKEFLWNIQKVQKGKDIRLSLDSKLQQKSEEALRSQIKKLPDA
CCCCCCCCCCEEEEEECCCCEEEEHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCC
KAGYAVVSDAKTGAILTMANSAVFDPNILHTHVSTKIDNLKSLSQNKAIQKLKYGESYIN
CCCEEEEECCCCCEEEEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
MASTIKPLTILIGLNEKLFQPEDTYLDKGTFEYDNQNNITNAPGTPTGEITPRQAIINSS
HHHCCCEEEEEEECCHHHCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCHHHHCCCC
NTFMTAKVALPLFNQHNGNVEKVAHIWTDYLMQFGLRSKTGIDLPFEEDGQYEFHPSNKF
CCEEEEEEEEEHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCHH
ENGISALLNASWGGNEVHTPLQLAQYAATLARKGDKYKPQMTSAIIGQNGKETKKFKPIL
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCHHHHHHHHH
ESSNRYPTKFWSVVQGGMSQNIEEIKKLPFDVAGKTGITGFPDEQARMINHSLFIAYAPT
HCCCCCCHHHHHHHHCHHHCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCEEEEEECCC
EDPQIAVSVVIPGSNSEKNIAALVTSEILESWNTLQKENKNKKEGSLK
CCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]