| Definition | Bacillus anthracis str. CDC 684, complete genome. |
|---|---|
| Accession | NC_012581 |
| Length | 5,230,115 |
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The map label for this gene is pulA [H]
Identifier: 227814459
GI number: 227814459
Start: 1694849
End: 1697407
Strand: Direct
Name: pulA [H]
Synonym: BAMEG_1867
Alternate gene names: 227814459
Gene position: 1694849-1697407 (Clockwise)
Preceding gene: 227814457
Following gene: 227814460
Centisome position: 32.41
GC content: 34.47
Gene sequence:
>2559_bases GTGCAAATTACAAAAAGATTAATTAACAAAACAGTTTTATTACTTACTTTAATCGTTATGTTATCATCGATTTTCTCCTT TCAGAGTGTGAAGGCAGTTTCAAATTCGAAAACAACTGAAGTCATCATTCATTACAAAGAGCAGTCTGGAAATACAAAAG ACTGGAACCTTTGGATATGGGGAGAAAATTCAAGTGGAAAATCATATGAATTTACTGGTGAAGATGAATTTGGAAAGTAC GCAAAGATTAATATAGATGGTGATTATAACCGTTTAGGATTTATCATTCGTACAAATGAGTGGGAAAAAGATGGTGGGGA TCGTTGGATTGAAAATATAAAAGACGGTCGTGCTGAAGTATGGATTCTTTCGGGTGATGAAAAAGTGTATAATTCTAAGC CGTCATCGGATCTCTCAATTCAAAAAGCAACTATTGATAGTTTCCATGAAATTACTGTAACGACGAATGTCCCATTTAAT ATTAAGGAAAAGAAAATTGAAATGGAAGGTATTAAAATTAAGAGTATTAGTCCTTATGATATAAACAGTGGAGATATTAC GAATAAGGTTAAAATAATTACGGAACAGAAAATTGATTTAAAACAAACATACAAAGTTAAAATAGAAAACTTAGCTGATA CGAATACTGAAATCGGTAAAGTCATTCGTAGTGAGGAGTTTGACTATTTATTTTATTACGGTGGCAACGATTTAGGTAAT ATATATACACCACAACATACAAAGTTCCGTGTATGGGCTCCTACTGCGAGTGAAGCGAAGTTAGTTACATATAAAAAATG GAACGATAAAATAGGTACTGAAATAAATATGCAACAAGGTGAAAAGGGCACCTGGAAAGCAGAACTTAAAGGGAATCAAA AAGGTCTCTATTATACGTATAAGGTGAAAATTGGTGATAAGTGGACAGAGGCCGTTGATCCGTATGCACGTGCTGCTTCT GTAAATGGAGATAAAGGTGCGGTTGTTGATTTAGAAGAAACAAATCCGAAAAAATGGAACACTAACAAAAAGCCTAAATT GAAAAATCCGGAAGATGCTATTATTTATGAGTTACATGTACGCGATCTTTCTATTCAGCCCGAAAGTGGTATTAAACAGA AAGGGAAGTATTTAGGTGTAACAGAAAAAGGAACAAAAGGGCCTGAAGGTGTGAAAACGGGACTTGATCATATGAAAGAT CTTGGTGTTACACACGTTCAGCTTTTACCAATATTTGATTATGCTTCAGTAAATGAAGAGAAGGTAAACGAACCACAATA TAACTGGGGATATGACCCAAAAAACTTTAACGTTCCAGAGGGGTCCTATTCTACAAATCCATATGAGCCAACTGTTCGAA TAACTGAATTAAAACAAATGATTCAAACACTACATGATAATAATCTTCGAGTGGTGATGGATGTAGTATATAACCATATG TATAACGCTGCTGAATCTAATTTCCATAAGTTGGTTCCAGGTTATTATTATCGTTACAATGAAGATGGAACATTTGCAAA TGGAACTGGAGTAGGAAATGATACGGCTTCAGAAAGAACAATGATGAGGAAATTTATGATAGATTCCGTCACATATTGGG CAAAAGAATACAATTTAGATGGATTCCGTTTTGATTTAATGGGTATTCATGATTATGAAACGATGAATGAAATACGTAAA GCTGTTAATCAAATTGACCCTTCTATTATACTGCATGGAGAAGGTTGGGATTTAAATACACCCCTTGCAGCTGAGTTGAA AGCAAATCAAAAAAATGCGGAGAAAATGAAAGGAATTGCCCATTTTAACGATAATATACGTGATGGATTGAAAGGTAGTG TGTTTGAAGAGAAAGAAAATGGATTTGTAAATGGAAAGGAAAACATGGAAGACCGTATTAAAAAAGGTATTACGGCAGGT ATTGACTATGATACAAATTCGTCTACCTATCAAGACCCGGAACAGGTATTAACTTATGTGGAAGCACATGACAATCATAC ATTATGGGATAAACTTGAGTTAACTAATCCAGGTGACAGTGAAGAAGTGCGAAAACAAATACACAAATTGTCTTCTTCAA TTTTATTAACATCACAAGGAATTCCATTTTTACATGCAGGGCAAGAGTTTATGCGGACGAAATACGGTGATCATAATAGT TACAAATCTCCAGATTCGATTAACCAGATGGATTGGCTGCGCCGTGCAACGTTTAATAATGAAGTAGATTATATGAAGGG TTTAATAGAATTACGCAAAAAGTACCCAGCTTTTCGAATGACATCTGCAGAGCAAATAAAAACACACGTATCATTTATTG ATGCTCCGAAAAATACTGTAACTTATACAATAGAGGGGAATAAACATGAATACTTTACAGTGGCTCACAATGCAAATAGA GAAGCTGTTGAAATAACACTTCCATCTAAAGGGCCTTGGAAAGTACTAGTAGATGGCAAGCAGGCGGGAAGTAAACCACT TTATGTTGTCCATGACAATAAAATTAAAGTTCCTGCATTAAGTTCCATTGTTTTAAAATCAGAAAAACCGATTAAATAA
Upstream 100 bases:
>100_bases ACGGGGGTGAAGCAAAAGAGATTTCTAGATTAATTTAAATTGTGTAATGTAGTACGGGCATAATAAATCGTTCGTACTAC TTAAAGAAAGGGTGTATGTG
Downstream 100 bases:
>100_bases AAGCGAAGAGTGTGGAAATAAATACTGAATTGAGCAAAGTTAATAAGGATTGCAAATGAAAAAAGCGAAGTGAAGATTAT TCTCACTTCGTTTTTAAAGT
Product: putative pullulanase
Products: NA
Alternate protein names: Alpha-dextrin endo-1,6-alpha-glucosidase; Pullulan 6-glucanohydrolase [H]
Number of amino acids: Translated: 852; Mature: 852
Protein sequence:
>852_residues MQITKRLINKTVLLLTLIVMLSSIFSFQSVKAVSNSKTTEVIIHYKEQSGNTKDWNLWIWGENSSGKSYEFTGEDEFGKY AKINIDGDYNRLGFIIRTNEWEKDGGDRWIENIKDGRAEVWILSGDEKVYNSKPSSDLSIQKATIDSFHEITVTTNVPFN IKEKKIEMEGIKIKSISPYDINSGDITNKVKIITEQKIDLKQTYKVKIENLADTNTEIGKVIRSEEFDYLFYYGGNDLGN IYTPQHTKFRVWAPTASEAKLVTYKKWNDKIGTEINMQQGEKGTWKAELKGNQKGLYYTYKVKIGDKWTEAVDPYARAAS VNGDKGAVVDLEETNPKKWNTNKKPKLKNPEDAIIYELHVRDLSIQPESGIKQKGKYLGVTEKGTKGPEGVKTGLDHMKD LGVTHVQLLPIFDYASVNEEKVNEPQYNWGYDPKNFNVPEGSYSTNPYEPTVRITELKQMIQTLHDNNLRVVMDVVYNHM YNAAESNFHKLVPGYYYRYNEDGTFANGTGVGNDTASERTMMRKFMIDSVTYWAKEYNLDGFRFDLMGIHDYETMNEIRK AVNQIDPSIILHGEGWDLNTPLAAELKANQKNAEKMKGIAHFNDNIRDGLKGSVFEEKENGFVNGKENMEDRIKKGITAG IDYDTNSSTYQDPEQVLTYVEAHDNHTLWDKLELTNPGDSEEVRKQIHKLSSSILLTSQGIPFLHAGQEFMRTKYGDHNS YKSPDSINQMDWLRRATFNNEVDYMKGLIELRKKYPAFRMTSAEQIKTHVSFIDAPKNTVTYTIEGNKHEYFTVAHNANR EAVEITLPSKGPWKVLVDGKQAGSKPLYVVHDNKIKVPALSSIVLKSEKPIK
Sequences:
>Translated_852_residues MQITKRLINKTVLLLTLIVMLSSIFSFQSVKAVSNSKTTEVIIHYKEQSGNTKDWNLWIWGENSSGKSYEFTGEDEFGKY AKINIDGDYNRLGFIIRTNEWEKDGGDRWIENIKDGRAEVWILSGDEKVYNSKPSSDLSIQKATIDSFHEITVTTNVPFN IKEKKIEMEGIKIKSISPYDINSGDITNKVKIITEQKIDLKQTYKVKIENLADTNTEIGKVIRSEEFDYLFYYGGNDLGN IYTPQHTKFRVWAPTASEAKLVTYKKWNDKIGTEINMQQGEKGTWKAELKGNQKGLYYTYKVKIGDKWTEAVDPYARAAS VNGDKGAVVDLEETNPKKWNTNKKPKLKNPEDAIIYELHVRDLSIQPESGIKQKGKYLGVTEKGTKGPEGVKTGLDHMKD LGVTHVQLLPIFDYASVNEEKVNEPQYNWGYDPKNFNVPEGSYSTNPYEPTVRITELKQMIQTLHDNNLRVVMDVVYNHM YNAAESNFHKLVPGYYYRYNEDGTFANGTGVGNDTASERTMMRKFMIDSVTYWAKEYNLDGFRFDLMGIHDYETMNEIRK AVNQIDPSIILHGEGWDLNTPLAAELKANQKNAEKMKGIAHFNDNIRDGLKGSVFEEKENGFVNGKENMEDRIKKGITAG IDYDTNSSTYQDPEQVLTYVEAHDNHTLWDKLELTNPGDSEEVRKQIHKLSSSILLTSQGIPFLHAGQEFMRTKYGDHNS YKSPDSINQMDWLRRATFNNEVDYMKGLIELRKKYPAFRMTSAEQIKTHVSFIDAPKNTVTYTIEGNKHEYFTVAHNANR EAVEITLPSKGPWKVLVDGKQAGSKPLYVVHDNKIKVPALSSIVLKSEKPIK >Mature_852_residues MQITKRLINKTVLLLTLIVMLSSIFSFQSVKAVSNSKTTEVIIHYKEQSGNTKDWNLWIWGENSSGKSYEFTGEDEFGKY AKINIDGDYNRLGFIIRTNEWEKDGGDRWIENIKDGRAEVWILSGDEKVYNSKPSSDLSIQKATIDSFHEITVTTNVPFN IKEKKIEMEGIKIKSISPYDINSGDITNKVKIITEQKIDLKQTYKVKIENLADTNTEIGKVIRSEEFDYLFYYGGNDLGN IYTPQHTKFRVWAPTASEAKLVTYKKWNDKIGTEINMQQGEKGTWKAELKGNQKGLYYTYKVKIGDKWTEAVDPYARAAS VNGDKGAVVDLEETNPKKWNTNKKPKLKNPEDAIIYELHVRDLSIQPESGIKQKGKYLGVTEKGTKGPEGVKTGLDHMKD LGVTHVQLLPIFDYASVNEEKVNEPQYNWGYDPKNFNVPEGSYSTNPYEPTVRITELKQMIQTLHDNNLRVVMDVVYNHM YNAAESNFHKLVPGYYYRYNEDGTFANGTGVGNDTASERTMMRKFMIDSVTYWAKEYNLDGFRFDLMGIHDYETMNEIRK AVNQIDPSIILHGEGWDLNTPLAAELKANQKNAEKMKGIAHFNDNIRDGLKGSVFEEKENGFVNGKENMEDRIKKGITAG IDYDTNSSTYQDPEQVLTYVEAHDNHTLWDKLELTNPGDSEEVRKQIHKLSSSILLTSQGIPFLHAGQEFMRTKYGDHNS YKSPDSINQMDWLRRATFNNEVDYMKGLIELRKKYPAFRMTSAEQIKTHVSFIDAPKNTVTYTIEGNKHEYFTVAHNANR EAVEITLPSKGPWKVLVDGKQAGSKPLYVVHDNKIKVPALSSIVLKSEKPIK
Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]
COG id: COG1523
COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the glycosyl hydrolase 13 family [H]
Homologues:
Organism=Escherichia coli, GI2367229, Length=665, Percent_Identity=22.5563909774436, Blast_Score=120, Evalue=3e-28,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR006047 - InterPro: IPR004193 - InterPro: IPR017853 - InterPro: IPR013781 - InterPro: IPR013783 - InterPro: IPR014756 - InterPro: IPR005323 - InterPro: IPR011840 [H]
Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48; PF03714 PUD [H]
EC number: =3.2.1.41 [H]
Molecular weight: Translated: 97294; Mature: 97294
Theoretical pI: Translated: 6.91; Mature: 6.91
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.0 %Cys (Translated Protein) 2.2 %Met (Translated Protein) 2.2 %Cys+Met (Translated Protein) 0.0 %Cys (Mature Protein) 2.2 %Met (Mature Protein) 2.2 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MQITKRLINKTVLLLTLIVMLSSIFSFQSVKAVSNSKTTEVIIHYKEQSGNTKDWNLWIW CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCEEEEEE GENSSGKSYEFTGEDEFGKYAKINIDGDYNRLGFIIRTNEWEKDGGDRWIENIKDGRAEV ECCCCCCEEEECCCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCHHHHHHHHCCCCEEE WILSGDEKVYNSKPSSDLSIQKATIDSFHEITVTTNVPFNIKEKKIEMEGIKIKSISPYD EEECCCCCCCCCCCCCCCEEEEEECCCEEEEEEEECCCCCCHHCEEEECCEEEECCCCCC INSGDITNKVKIITEQKIDLKQTYKVKIENLADTNTEIGKVIRSEEFDYLFYYGGNDLGN CCCCCCCCEEEEEEECCCCCCEEEEEEEECCCCCCHHHHHHHHCCCCCEEEEECCCCCCC IYTPQHTKFRVWAPTASEAKLVTYKKWNDKIGTEINMQQGEKGTWKAELKGNQKGLYYTY CCCCCCCEEEEECCCCCCCEEEEEEECCCCCCCEEECCCCCCCCEEEEECCCCCCEEEEE KVKIGDKWTEAVDPYARAASVNGDKGAVVDLEETNPKKWNTNKKPKLKNPEDAIIYELHV EEEECCCHHHHCCHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEEE RDLSIQPESGIKQKGKYLGVTEKGTKGPEGVKTGLDHMKDLGVTHVQLLPIFDYASVNEE EEEEECCCCCHHHCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHH KVNEPQYNWGYDPKNFNVPEGSYSTNPYEPTVRITELKQMIQTLHDNNLRVVMDVVYNHM HCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHCCCCCEEHHHHHHHHH YNAAESNFHKLVPGYYYRYNEDGTFANGTGVGNDTASERTMMRKFMIDSVTYWAKEYNLD HHHHHCCHHHCCCCEEEEECCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC GFRFDLMGIHDYETMNEIRKAVNQIDPSIILHGEGWDLNTPLAAELKANQKNAEKMKGIA CEEEEEEECCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHEECCCCCHHHHHHHHH HFNDNIRDGLKGSVFEEKENGFVNGKENMEDRIKKGITAGIDYDTNSSTYQDPEQVLTYV HCCCCHHCCCCCCCEECCCCCCCCCCCCHHHHHHCCCEECCCCCCCCCCCCCHHHHHHHH EAHDNHTLWDKLELTNPGDSEEVRKQIHKLSSSILLTSQGIPFLHAGQEFMRTKYGDHNS HCCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCEEEEECCCCCEECCHHHHHHHCCCCCC YKSPDSINQMDWLRRATFNNEVDYMKGLIELRKKYPAFRMTSAEQIKTHVSFIDAPKNTV CCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHEEECCCCEE TYTIEGNKHEYFTVAHNANREAVEITLPSKGPWKVLVDGKQAGSKPLYVVHDNKIKVPAL EEEEECCCEEEEEEEECCCCEEEEEECCCCCCEEEEEECCCCCCCCEEEEECCEECCCCH SSIVLKSEKPIK HHEEECCCCCCC >Mature Secondary Structure MQITKRLINKTVLLLTLIVMLSSIFSFQSVKAVSNSKTTEVIIHYKEQSGNTKDWNLWIW CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCEEEEEE GENSSGKSYEFTGEDEFGKYAKINIDGDYNRLGFIIRTNEWEKDGGDRWIENIKDGRAEV ECCCCCCEEEECCCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCHHHHHHHHCCCCEEE WILSGDEKVYNSKPSSDLSIQKATIDSFHEITVTTNVPFNIKEKKIEMEGIKIKSISPYD EEECCCCCCCCCCCCCCCEEEEEECCCEEEEEEEECCCCCCHHCEEEECCEEEECCCCCC INSGDITNKVKIITEQKIDLKQTYKVKIENLADTNTEIGKVIRSEEFDYLFYYGGNDLGN CCCCCCCCEEEEEEECCCCCCEEEEEEEECCCCCCHHHHHHHHCCCCCEEEEECCCCCCC IYTPQHTKFRVWAPTASEAKLVTYKKWNDKIGTEINMQQGEKGTWKAELKGNQKGLYYTY CCCCCCCEEEEECCCCCCCEEEEEEECCCCCCCEEECCCCCCCCEEEEECCCCCCEEEEE KVKIGDKWTEAVDPYARAASVNGDKGAVVDLEETNPKKWNTNKKPKLKNPEDAIIYELHV EEEECCCHHHHCCHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEEE RDLSIQPESGIKQKGKYLGVTEKGTKGPEGVKTGLDHMKDLGVTHVQLLPIFDYASVNEE EEEEECCCCCHHHCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHH KVNEPQYNWGYDPKNFNVPEGSYSTNPYEPTVRITELKQMIQTLHDNNLRVVMDVVYNHM HCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHCCCCCEEHHHHHHHHH YNAAESNFHKLVPGYYYRYNEDGTFANGTGVGNDTASERTMMRKFMIDSVTYWAKEYNLD HHHHHCCHHHCCCCEEEEECCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC GFRFDLMGIHDYETMNEIRKAVNQIDPSIILHGEGWDLNTPLAAELKANQKNAEKMKGIA CEEEEEEECCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHEECCCCCHHHHHHHHH HFNDNIRDGLKGSVFEEKENGFVNGKENMEDRIKKGITAGIDYDTNSSTYQDPEQVLTYV HCCCCHHCCCCCCCEECCCCCCCCCCCCHHHHHHCCCEECCCCCCCCCCCCCHHHHHHHH EAHDNHTLWDKLELTNPGDSEEVRKQIHKLSSSILLTSQGIPFLHAGQEFMRTKYGDHNS HCCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCEEEEECCCCCEECCHHHHHHHCCCCCC YKSPDSINQMDWLRRATFNNEVDYMKGLIELRKKYPAFRMTSAEQIKTHVSFIDAPKNTV CCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHEEECCCCEE TYTIEGNKHEYFTVAHNANREAVEITLPSKGPWKVLVDGKQAGSKPLYVVHDNKIKVPAL EEEEECCCEEEEEEEECCCCEEEEEECCCCCCEEEEEECCCCCCCCEEEEECCEECCCCH SSIVLKSEKPIK HHEEECCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 9453151; 10360571 [H]