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The map label for this gene is gppA

Identifier: 227080518

GI number: 227080518

Start: 299569

End: 301062

Strand: Reverse

Name: gppA

Synonym: VCM66_0289

Alternate gene names: 227080518

Gene position: 301062-299569 (Counterclockwise)

Preceding gene: 227080519

Following gene: 227080515

Centisome position: 10.41

GC content: 50.2

Gene sequence:

>1494_bases
ATGAGCCAAGCCGTTTCTTCACCCTTGTATGCTGCCATTGATTTAGGTTCCAACAGTTTTCACATGTTGGTCGTGCGTCA
TATCGATGGCAGTGTGCAGACAATGGCAAAAATCAAACGTAAGGTGCGTCTGGCAGCCGGTCTAGATGAACACAACGCCT
TGAGCTTAGATGCGATGCAAAGAGGGTGGGATTGTCTCAGCCTTTTTGCCGAGCGACTGCAAGATATTCCCGCAGAGAAT
ATCCGAATTGTAGGGACCGCAACCCTGCGTACCGCCACCAATGCCGGTGAATTTATCGCTAAAGCGAACCAGATTTTAGG
CCATCCGATCGACGTTATTTCCGGTGAAGAAGAAGCGGCCACTATCTATAAAGGAGTCGCGCACACTTCGGGAGGTTTAG
GGCGTCGCTTAGTAGTGGATATTGGTGGTGCGAGTACCGAGCTGATTATCGGTGAAGGTTTTGAAGCCAAAGCCCTCACC
AGCTTGAAAATGGGTTGTGTGACTTGGTTAGAGCGCCATTTCAAAGATCGTCAGCTGACGGCCACCAACTTTAATAACGC
CATTTTAGCCGCCAAACAGATGCTGGATCCGATCCTCACTCAATACACTGAGCTTGGCTGGAATGTGTGTGTTGGAGCCA
GTGGTACGGTACAAGCACTGCAAGAGATCATGCTGGCGCAAGGCATGGATGAAGTCATTACGCTGACCAAACTCAAGCGT
TTACAAAAGCAAGCCATGCTGGCCGATCATCTCGAAGAGCTGGATATCGAAGGGTTAACGCTGGAACGTGCTCTGGTTTT
CCCAAGTGGCCTATCGATTCTGATTGCGATTTTTGAATCACTGAACATCGAAGCGATGACGCTAGCGGGCGGTGCTCTGC
GTGAAGGTCTCGTCTATGAGATGGTTCAAGACCTACGCCAAGAAGATATTCGAGCGCGCACCATTCGCTGTGTACAAACC
CGTTATCAGATCGATAGCGCTTATGGTGATCAAGTCGCCACTCTCGCCAGTAAACTGCTCGCGCAATGTGGCGGTGAAGC
GTGGATCAATGAACCACAAGCAGAGATGTTGCTGCGTACGGCAGCTAAGTTGCATGAAATTGGTCTCACCATCGATTTCA
AAAAAGGGGGGGAGCACAGCGCTTACCTACTCCAACATCTTGATCTGCCCGGTTATACCCGCGCCCAAAAACATTATTTA
GGTGAAATCGTACGTCGCTATCGCGAACAGTTAACTTCGTTACCTGAACAATATGCACTGTCAGGCACCAGTGGTAAACG
CGTACTGCGTTTGCTGCGCCTTGCTGTCCTCTTAAGCCATCGACGCAGCCCGGCGTTAGAGCCGATGGTAGAATTGAGCG
CACAAGAGGACAAGCTGACGCTCACGCTTGACGGTGAATGGCTGGCAAAAAATCCACTGACTCGTACAGAGTTAGAGTTA
GAAGCCAACCGTCAAACCGATATTGGCTGGCCTCTGTCGATTGAATGCCACTAA

Upstream 100 bases:

>100_bases
AATACCGCAGGTTCTCGCAATAGCAATAATGGTGGCAATCGCAAACCTCAGCAACGCCGCCCACGCGCACCACGCCCGAA
AAAGGAAGCCTGATTTCGCT

Downstream 100 bases:

>100_bases
GCACTCATAAGTATTAAGCCCTCATCTGAGGGCTTAATACTTACTGTACCTTCAATATTCCTAAACGACTTGAAGTTGCA
AGTAGGCAGCGTATAGACGG

Product: guanosine pentaphosphate phosphohydrolase

Products: NA

Alternate protein names: Guanosine pentaphosphate phosphohydrolase; pppGpp-5'-phosphohydrolase

Number of amino acids: Translated: 497; Mature: 496

Protein sequence:

>497_residues
MSQAVSSPLYAAIDLGSNSFHMLVVRHIDGSVQTMAKIKRKVRLAAGLDEHNALSLDAMQRGWDCLSLFAERLQDIPAEN
IRIVGTATLRTATNAGEFIAKANQILGHPIDVISGEEEAATIYKGVAHTSGGLGRRLVVDIGGASTELIIGEGFEAKALT
SLKMGCVTWLERHFKDRQLTATNFNNAILAAKQMLDPILTQYTELGWNVCVGASGTVQALQEIMLAQGMDEVITLTKLKR
LQKQAMLADHLEELDIEGLTLERALVFPSGLSILIAIFESLNIEAMTLAGGALREGLVYEMVQDLRQEDIRARTIRCVQT
RYQIDSAYGDQVATLASKLLAQCGGEAWINEPQAEMLLRTAAKLHEIGLTIDFKKGGEHSAYLLQHLDLPGYTRAQKHYL
GEIVRRYREQLTSLPEQYALSGTSGKRVLRLLRLAVLLSHRRSPALEPMVELSAQEDKLTLTLDGEWLAKNPLTRTELEL
EANRQTDIGWPLSIECH

Sequences:

>Translated_497_residues
MSQAVSSPLYAAIDLGSNSFHMLVVRHIDGSVQTMAKIKRKVRLAAGLDEHNALSLDAMQRGWDCLSLFAERLQDIPAEN
IRIVGTATLRTATNAGEFIAKANQILGHPIDVISGEEEAATIYKGVAHTSGGLGRRLVVDIGGASTELIIGEGFEAKALT
SLKMGCVTWLERHFKDRQLTATNFNNAILAAKQMLDPILTQYTELGWNVCVGASGTVQALQEIMLAQGMDEVITLTKLKR
LQKQAMLADHLEELDIEGLTLERALVFPSGLSILIAIFESLNIEAMTLAGGALREGLVYEMVQDLRQEDIRARTIRCVQT
RYQIDSAYGDQVATLASKLLAQCGGEAWINEPQAEMLLRTAAKLHEIGLTIDFKKGGEHSAYLLQHLDLPGYTRAQKHYL
GEIVRRYREQLTSLPEQYALSGTSGKRVLRLLRLAVLLSHRRSPALEPMVELSAQEDKLTLTLDGEWLAKNPLTRTELEL
EANRQTDIGWPLSIECH
>Mature_496_residues
SQAVSSPLYAAIDLGSNSFHMLVVRHIDGSVQTMAKIKRKVRLAAGLDEHNALSLDAMQRGWDCLSLFAERLQDIPAENI
RIVGTATLRTATNAGEFIAKANQILGHPIDVISGEEEAATIYKGVAHTSGGLGRRLVVDIGGASTELIIGEGFEAKALTS
LKMGCVTWLERHFKDRQLTATNFNNAILAAKQMLDPILTQYTELGWNVCVGASGTVQALQEIMLAQGMDEVITLTKLKRL
QKQAMLADHLEELDIEGLTLERALVFPSGLSILIAIFESLNIEAMTLAGGALREGLVYEMVQDLRQEDIRARTIRCVQTR
YQIDSAYGDQVATLASKLLAQCGGEAWINEPQAEMLLRTAAKLHEIGLTIDFKKGGEHSAYLLQHLDLPGYTRAQKHYLG
EIVRRYREQLTSLPEQYALSGTSGKRVLRLLRLAVLLSHRRSPALEPMVELSAQEDKLTLTLDGEWLAKNPLTRTELELE
ANRQTDIGWPLSIECH

Specific function: Conversion of pppGpp to ppGpp. Guanosine pentaphosphate (pppGpp) is a cytoplasmic signaling molecule which together with ppGpp controls the "stringent response", an adaptive process that allows bacteria to respond to amino acid starvation, resulting in th

COG id: COG0248

COG function: function code FP; Exopolyphosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gppA/ppx family. GppA subfamily

Homologues:

Organism=Escherichia coli, GI48994966, Length=491, Percent_Identity=56.8228105906314, Blast_Score=539, Evalue=1e-154,
Organism=Escherichia coli, GI1788848, Length=492, Percent_Identity=40.8536585365854, Blast_Score=361, Evalue=1e-101,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GPPA_VIBC3 (A5F3R3)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F3R3
- SMR:   A5F3R3
- STRING:   A5F3R3
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A2696
- eggNOG:   COG0248
- HOGENOM:   HBG451789
- OMA:   AMQRGWQ
- ProtClustDB:   PRK11031
- BioCyc:   VCHO345073:VC0395_A2696-MONOMER
- HAMAP:   MF_01550
- InterPro:   IPR003695

Pfam domain/function: PF02541 Ppx-GppA

EC number: =3.6.1.40

Molecular weight: Translated: 54827; Mature: 54696

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQAVSSPLYAAIDLGSNSFHMLVVRHIDGSVQTMAKIKRKVRLAAGLDEHNALSLDAMQ
CCCCCCCCCEEEEECCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCEEHHHHH
RGWDCLSLFAERLQDIPAENIRIVGTATLRTATNAGEFIAKANQILGHPIDVISGEEEAA
CCHHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCHHHHHHHHHHHHCCCHHHCCCCHHHH
TIYKGVAHTSGGLGRRLVVDIGGASTELIIGEGFEAKALTSLKMGCVTWLERHFKDRQLT
HHHHHHHHCCCCCCCEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEE
ATNFNNAILAAKQMLDPILTQYTELGWNVCVGASGTVQALQEIMLAQGMDEVITLTKLKR
ECCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHH
LQKQAMLADHLEELDIEGLTLERALVFPSGLSILIAIFESLNIEAMTLAGGALREGLVYE
HHHHHHHHHHHHHCCCCCEEEEHHEECCCHHHHHHHHHHHCCCEEEEECCHHHHHHHHHH
MVQDLRQEDIRARTIRCVQTRYQIDSAYGDQVATLASKLLAQCGGEAWINEPQAEMLLRT
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
AAKLHEIGLTIDFKKGGEHSAYLLQHLDLPGYTRAQKHYLGEIVRRYREQLTSLPEQYAL
HHHHHHHCCEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCC
SGTSGKRVLRLLRLAVLLSHRRSPALEPMVELSAQEDKLTLTLDGEWLAKNPLTRTELEL
CCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCEEEEEECCCCCCCCCCCCCEEEE
EANRQTDIGWPLSIECH
ECCCCCCCCCCEEEEEC
>Mature Secondary Structure 
SQAVSSPLYAAIDLGSNSFHMLVVRHIDGSVQTMAKIKRKVRLAAGLDEHNALSLDAMQ
CCCCCCCCEEEEECCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCEEHHHHH
RGWDCLSLFAERLQDIPAENIRIVGTATLRTATNAGEFIAKANQILGHPIDVISGEEEAA
CCHHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCHHHHHHHHHHHHCCCHHHCCCCHHHH
TIYKGVAHTSGGLGRRLVVDIGGASTELIIGEGFEAKALTSLKMGCVTWLERHFKDRQLT
HHHHHHHHCCCCCCCEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEE
ATNFNNAILAAKQMLDPILTQYTELGWNVCVGASGTVQALQEIMLAQGMDEVITLTKLKR
ECCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHH
LQKQAMLADHLEELDIEGLTLERALVFPSGLSILIAIFESLNIEAMTLAGGALREGLVYE
HHHHHHHHHHHHHCCCCCEEEEHHEECCCHHHHHHHHHHHCCCEEEEECCHHHHHHHHHH
MVQDLRQEDIRARTIRCVQTRYQIDSAYGDQVATLASKLLAQCGGEAWINEPQAEMLLRT
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
AAKLHEIGLTIDFKKGGEHSAYLLQHLDLPGYTRAQKHYLGEIVRRYREQLTSLPEQYAL
HHHHHHHCCEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCC
SGTSGKRVLRLLRLAVLLSHRRSPALEPMVELSAQEDKLTLTLDGEWLAKNPLTRTELEL
CCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCEEEEEECCCCCCCCCCCCCEEEE
EANRQTDIGWPLSIECH
ECCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA