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The map label for this gene is rfbU [H]

Identifier: 227080475

GI number: 227080475

Start: 250101

End: 251966

Strand: Reverse

Name: rfbU [H]

Synonym: VCM66_0245

Alternate gene names: 227080475

Gene position: 251966-250101 (Counterclockwise)

Preceding gene: 227080476

Following gene: 227080474

Centisome position: 8.71

GC content: 43.46

Gene sequence:

>1866_bases
ATGTTTACCCCTATTCAACTGCTCTTAAGCGCACAGCGTCATCATAAGCGTATGATAACGTTGCTATATGATGTATTTGC
GCTGATGTTTTCCCTTTATGCTGCCATCGCACTACGCTTAGATACTTTAGCATTCACGTTTAGCTTCCCAGAGTTTCTGA
GCATGATGACAACGGTAGGGGTCACTCTCTACTGCTTTGTCCGACTCGGTATGTATCGCGCAGTGCTACGTTATATGATG
CTGCCTGCGATCGGCTATATCTTTTTGTCTGTCATCATTTCTGCGGTGACGCTAGCACTAAGCGGCTTTTTCTTTCAGGC
CTTTATTCCCCGCAGTGTCCCCTTTATTTATGCAGGCCTAGCAACGCTGACTTTAGGTGGGCCTAGAATTTTTATTCGCA
CCATCTATTACCATTATTACAAGCGTCAGAAACCAAATGTTTTTATCTATGGTGCAGGTTCAACAGGGCGAGATCTGGCC
TATGCTCTCATTCAAGGTGATGAGTACCACCCGGTAATCTTCTTAGATGATGATCCAGCTAAAACAGGACAAATTATATT
TGGGCTGCGAGTGCACCATAGTAGTGATTTTGAATCACTCAGAAATCTTTATGAACCCGTAAAGATGCTGATCGCCATAA
ATAATATCGGTAAAAATACACGTATAAAATTACTTGAACGACTCTCTCATTGGCCCATTGAGATTCAATCTGTCCCCTCA
GTGGAAGATATTGCTGCGGGACGCGCAAAAGCGACTGATGTACAAGATCTTGATATCGCAGATTTGCTTGGCCGCGCACC
GGTTGAACCCGACTTAGCGCTGTTACAAAAAAACATTACTGAAAAATCCGTCATGGTCACTGGAGCGGGAGGTTCGATTG
GCTCAGAGTTATGCAGACAAATCTTGGCTCAAAAACCTAAAACCTTAGTGTTATTTGAGCTCAATGAATATAACTTGTAT
ACCATCGATCAGGAATTACAAGCGATCAAACAGAATTTAAAACTCAACACGAAAATAGTTGCAGCACTTGGCTCAGTACA
ACGTGAAAACCGCGTCAAAAAACTCATGCAAGCTCATCAGGTTGAAACGGTTTACCATGCTGCAGCTTATAAACACGTTC
CTCTCGTTGAAGATAATGTTATCGAAGGCATTCGCAACAATGTCTTTGGAACATTAGCCTGTGCTAATGCAGCTATAGAA
GCTGGCGTAAAAAACTTTACTCTGATTTCAACGGATAAAGCCGTGCGGCCAACGAATATCATGGGAGCAAGCAAGCGGAT
GGCAGAGCTGGTACTACAAGCCCTAGCCGATAAAAACAGTACAACCATTTTTACCATGGTACGTTTCGGGAATGTCTTAG
GCTCTTCAGGTTCGGTCGTTCCTCTATTCAAAAAACAGATCCGCGCAGGTGGGCCAGTAACGGTAACTCACCCTGATATA
ATCCGATATTTCATGCTGATTCCAGAAGCCGCTCAGCTTGTCATTCAAGCTGAGGCGATGAGTCATAATGGACAAGTCTT
TGTCCTCGATATGGGTGAACCAGTAAAAATCGTCGATCTCGCCAAACGTATGATTCATCTTATGGGCATGAAAGAATTCT
GCGATGGAAGAAGTGATGAAGGCGATATCGAGATCAAGTTTACTGGCTTGCGCCCAGGAGAAAAGCTCTATGAAGAGCTA
CTTATCGGAGAGAATGTGGAAGGCACTAGCCATCAAAAGATCATGACAGCATGTGAAGAAAAACTGAGTTGGGATGCCAT
GGAAGACCTACTCACGGAACTTGATGTATGCTGTCATAACTTTGATGTTGAATGTATTAAGCGAATTTTACTTGATGCAC
CTACGGGTTATTCGCCACAAAAATGA

Upstream 100 bases:

>100_bases
CAAACGCTACTCAAGCAAACTACAATTCGGGCGATACTAATATAACGCATAACAATATCAACCCGTTGGGTTGGCTAATT
ATAAGGCGAGCTGCTAAGCA

Downstream 100 bases:

>100_bases
GAATAAAATGAAAGTATTGCATGTATATAGAACCTGTTATCCTGAAACAAAAGGCGGTGTTGAGCAAGTTATTCGATTTA
TAGCATCGGGAACAAAACCG

Product: mannosyl-transferase

Products: UDPglucoseal [C]

Alternate protein names: NA

Number of amino acids: Translated: 621; Mature: 621

Protein sequence:

>621_residues
MFTPIQLLLSAQRHHKRMITLLYDVFALMFSLYAAIALRLDTLAFTFSFPEFLSMMTTVGVTLYCFVRLGMYRAVLRYMM
LPAIGYIFLSVIISAVTLALSGFFFQAFIPRSVPFIYAGLATLTLGGPRIFIRTIYYHYYKRQKPNVFIYGAGSTGRDLA
YALIQGDEYHPVIFLDDDPAKTGQIIFGLRVHHSSDFESLRNLYEPVKMLIAINNIGKNTRIKLLERLSHWPIEIQSVPS
VEDIAAGRAKATDVQDLDIADLLGRAPVEPDLALLQKNITEKSVMVTGAGGSIGSELCRQILAQKPKTLVLFELNEYNLY
TIDQELQAIKQNLKLNTKIVAALGSVQRENRVKKLMQAHQVETVYHAAAYKHVPLVEDNVIEGIRNNVFGTLACANAAIE
AGVKNFTLISTDKAVRPTNIMGASKRMAELVLQALADKNSTTIFTMVRFGNVLGSSGSVVPLFKKQIRAGGPVTVTHPDI
IRYFMLIPEAAQLVIQAEAMSHNGQVFVLDMGEPVKIVDLAKRMIHLMGMKEFCDGRSDEGDIEIKFTGLRPGEKLYEEL
LIGENVEGTSHQKIMTACEEKLSWDAMEDLLTELDVCCHNFDVECIKRILLDAPTGYSPQK

Sequences:

>Translated_621_residues
MFTPIQLLLSAQRHHKRMITLLYDVFALMFSLYAAIALRLDTLAFTFSFPEFLSMMTTVGVTLYCFVRLGMYRAVLRYMM
LPAIGYIFLSVIISAVTLALSGFFFQAFIPRSVPFIYAGLATLTLGGPRIFIRTIYYHYYKRQKPNVFIYGAGSTGRDLA
YALIQGDEYHPVIFLDDDPAKTGQIIFGLRVHHSSDFESLRNLYEPVKMLIAINNIGKNTRIKLLERLSHWPIEIQSVPS
VEDIAAGRAKATDVQDLDIADLLGRAPVEPDLALLQKNITEKSVMVTGAGGSIGSELCRQILAQKPKTLVLFELNEYNLY
TIDQELQAIKQNLKLNTKIVAALGSVQRENRVKKLMQAHQVETVYHAAAYKHVPLVEDNVIEGIRNNVFGTLACANAAIE
AGVKNFTLISTDKAVRPTNIMGASKRMAELVLQALADKNSTTIFTMVRFGNVLGSSGSVVPLFKKQIRAGGPVTVTHPDI
IRYFMLIPEAAQLVIQAEAMSHNGQVFVLDMGEPVKIVDLAKRMIHLMGMKEFCDGRSDEGDIEIKFTGLRPGEKLYEEL
LIGENVEGTSHQKIMTACEEKLSWDAMEDLLTELDVCCHNFDVECIKRILLDAPTGYSPQK
>Mature_621_residues
MFTPIQLLLSAQRHHKRMITLLYDVFALMFSLYAAIALRLDTLAFTFSFPEFLSMMTTVGVTLYCFVRLGMYRAVLRYMM
LPAIGYIFLSVIISAVTLALSGFFFQAFIPRSVPFIYAGLATLTLGGPRIFIRTIYYHYYKRQKPNVFIYGAGSTGRDLA
YALIQGDEYHPVIFLDDDPAKTGQIIFGLRVHHSSDFESLRNLYEPVKMLIAINNIGKNTRIKLLERLSHWPIEIQSVPS
VEDIAAGRAKATDVQDLDIADLLGRAPVEPDLALLQKNITEKSVMVTGAGGSIGSELCRQILAQKPKTLVLFELNEYNLY
TIDQELQAIKQNLKLNTKIVAALGSVQRENRVKKLMQAHQVETVYHAAAYKHVPLVEDNVIEGIRNNVFGTLACANAAIE
AGVKNFTLISTDKAVRPTNIMGASKRMAELVLQALADKNSTTIFTMVRFGNVLGSSGSVVPLFKKQIRAGGPVTVTHPDI
IRYFMLIPEAAQLVIQAEAMSHNGQVFVLDMGEPVKIVDLAKRMIHLMGMKEFCDGRSDEGDIEIKFTGLRPGEKLYEEL
LIGENVEGTSHQKIMTACEEKLSWDAMEDLLTELDVCCHNFDVECIKRILLDAPTGYSPQK

Specific function: Required for the biosynthesis of type 1 capsular polysaccharide [H]

COG id: COG1086

COG function: function code MG; Predicted nucleoside-diphosphate sugar epimerases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR003869 [H]

Pfam domain/function: PF02719 Polysacc_synt_2 [H]

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 69304; Mature: 69304

Theoretical pI: Translated: 7.40; Mature: 7.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTPIQLLLSAQRHHKRMITLLYDVFALMFSLYAAIALRLDTLAFTFSFPEFLSMMTTVG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHH
VTLYCFVRLGMYRAVLRYMMLPAIGYIFLSVIISAVTLALSGFFFQAFIPRSVPFIYAGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
ATLTLGGPRIFIRTIYYHYYKRQKPNVFIYGAGSTGRDLAYALIQGDEYHPVIFLDDDPA
HHHCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHEEEEEECCCCCCEEEECCCCC
KTGQIIFGLRVHHSSDFESLRNLYEPVKMLIAINNIGKNTRIKLLERLSHWPIEIQSVPS
CCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEECCCC
VEDIAAGRAKATDVQDLDIADLLGRAPVEPDLALLQKNITEKSVMVTGAGGSIGSELCRQ
HHHHHCCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHCCCCCEEEEEECCCCHHHHHHHH
ILAQKPKTLVLFELNEYNLYTIDQELQAIKQNLKLNTKIVAALGSVQRENRVKKLMQAHQ
HHHCCCCEEEEEEECCCEEEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VETVYHAAAYKHVPLVEDNVIEGIRNNVFGTLACANAAIEAGVKNFTLISTDKAVRPTNI
HHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCC
MGASKRMAELVLQALADKNSTTIFTMVRFGNVLGSSGSVVPLFKKQIRAGGPVTVTHPDI
CCHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHCCCCCEEHHHHHHHCCCCCEEECCHHH
IRYFMLIPEAAQLVIQAEAMSHNGQVFVLDMGEPVKIVDLAKRMIHLMGMKEFCDGRSDE
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCHHHHCCCCCC
GDIEIKFTGLRPGEKLYEELLIGENVEGTSHQKIMTACEEKLSWDAMEDLLTELDVCCHN
CCEEEEEECCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHCC
FDVECIKRILLDAPTGYSPQK
CCHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MFTPIQLLLSAQRHHKRMITLLYDVFALMFSLYAAIALRLDTLAFTFSFPEFLSMMTTVG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHH
VTLYCFVRLGMYRAVLRYMMLPAIGYIFLSVIISAVTLALSGFFFQAFIPRSVPFIYAGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
ATLTLGGPRIFIRTIYYHYYKRQKPNVFIYGAGSTGRDLAYALIQGDEYHPVIFLDDDPA
HHHCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHEEEEEECCCCCCEEEECCCCC
KTGQIIFGLRVHHSSDFESLRNLYEPVKMLIAINNIGKNTRIKLLERLSHWPIEIQSVPS
CCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEECCCC
VEDIAAGRAKATDVQDLDIADLLGRAPVEPDLALLQKNITEKSVMVTGAGGSIGSELCRQ
HHHHHCCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHCCCCCEEEEEECCCCHHHHHHHH
ILAQKPKTLVLFELNEYNLYTIDQELQAIKQNLKLNTKIVAALGSVQRENRVKKLMQAHQ
HHHCCCCEEEEEEECCCEEEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VETVYHAAAYKHVPLVEDNVIEGIRNNVFGTLACANAAIEAGVKNFTLISTDKAVRPTNI
HHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCC
MGASKRMAELVLQALADKNSTTIFTMVRFGNVLGSSGSVVPLFKKQIRAGGPVTVTHPDI
CCHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHCCCCCEEHHHHHHHCCCCCEEECCHHH
IRYFMLIPEAAQLVIQAEAMSHNGQVFVLDMGEPVKIVDLAKRMIHLMGMKEFCDGRSDE
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCHHHHCCCCCC
GDIEIKFTGLRPGEKLYEELLIGENVEGTSHQKIMTACEEKLSWDAMEDLLTELDVCCHN
CCEEEEEECCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHCC
FDVECIKRILLDAPTGYSPQK
CCHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7961465 [H]