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The map label for this gene is rfbE [H]

Identifier: 227080463

GI number: 227080463

Start: 236443

End: 237546

Strand: Direct

Name: rfbE [H]

Synonym: VCM66_0232

Alternate gene names: 227080463

Gene position: 236443-237546 (Clockwise)

Preceding gene: 227080462

Following gene: 227080464

Centisome position: 8.17

GC content: 40.04

Gene sequence:

>1104_bases
ATGATTCCTGTATACGAACCAAGTTTGGATGGAAATGAGCGTAAATATCTAAACGATTGCATTGATTCCGGTTGGGTATC
CTCAAGGGGGAAATATATTGATCGCTTCGAAACTGAGTTTGCGGAGTTTTTAAAAGTAAAGCACGCCACAACAGTATCTA
ATGGAACAGTTGCGCTACATTTGGCAATGAGCGCGTTGGGAATAACTCAAGGCGATGAAGTGATTGTACCAACATTCACT
TATGTTGCCTCGGTTAATACCATAGTCCAGTGTGGTGCGTTACCCGTTTTTGCTGAAATCGAAGGTGAGTCTCTACAAGT
GAGCGTAGAGGACGTTAAACGTAAAATAAATAAAAAGACAAAAGCAGTTATGGCCGTTCACATATATGGACAAGCTTGCG
ATATTCAATCTTTAAGAGATCTGTGTGATGAGCACGGCCTCTATTTAATTGAAGACTGTGCAGAAGCAATAGGTACTGCT
GTTAATGGCAAAAAAGTAGGTACATTTGGCGATGTGTCAACGTTTAGTTTCTTTGGAAATAAAACCATCACATCGGGCGA
GGGTGGTATGGTTGTGTCAAACTCAGACATAATTATAGATAAATGTTTACGCTTAAAAAACCAAGGGGTAGTGGCAGGGA
AGCGCTACTGGCATGACCTAGTTGCGTATAACTATAGAATGACGAATCTATGTGCTGCGATTGGTGTTGCTCAGCTTGAA
AGAGTTGATAAAATTATAAAGCGCAAGCGTGATATAGCTGAAATTTATAGGTCTGAACTCGCTGGACTACCAATGCAAGT
GCACAAAGAGTCTAATGGAACTTTCCACTCATATTGGTTGACGTCTATTATATTAGACCAAGAGTTTGAAGTGCACCGCG
ATGGGTTGATGACCTTCTTAGAAAATAATGACATCGAGTCTAGACCATTTTTCTATCCTGCTCACACGCTGCCAATGTAC
GAGCATTTGGCAGAAAAGACAGCTTTTCCGTTATCAAATAGCTACTCTCATCGAGGTATTAATTTACCCAGTTGGCCAGG
GTTATGCGATGATCAAGTAAAAGAAATTTGCAACTGTATTAAAAATTATTTCAACTGTATTTGA

Upstream 100 bases:

>100_bases
TCTGAAATGGTAGCTTCTGATTTAGAACAAGCTCAAAGTCATGCACTATTGAAAAAACATGGCTATAACGTAAATGTATC
TGTAGAGTGAGGTCCTTTAA

Downstream 100 bases:

>100_bases
ATGTCATGACAGAAAAAAAGAAGCTAGTAGTTTGGGTTCCCTTACCTCCTAATTCATCTTGGCGTGGAGAGGGAATTGCT
CAAACAATTGAAAATATTGT

Product: perosamine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MIPVYEPSLDGNERKYLNDCIDSGWVSSRGKYIDRFETEFAEFLKVKHATTVSNGTVALHLAMSALGITQGDEVIVPTFT
YVASVNTIVQCGALPVFAEIEGESLQVSVEDVKRKINKKTKAVMAVHIYGQACDIQSLRDLCDEHGLYLIEDCAEAIGTA
VNGKKVGTFGDVSTFSFFGNKTITSGEGGMVVSNSDIIIDKCLRLKNQGVVAGKRYWHDLVAYNYRMTNLCAAIGVAQLE
RVDKIIKRKRDIAEIYRSELAGLPMQVHKESNGTFHSYWLTSIILDQEFEVHRDGLMTFLENNDIESRPFFYPAHTLPMY
EHLAEKTAFPLSNSYSHRGINLPSWPGLCDDQVKEICNCIKNYFNCI

Sequences:

>Translated_367_residues
MIPVYEPSLDGNERKYLNDCIDSGWVSSRGKYIDRFETEFAEFLKVKHATTVSNGTVALHLAMSALGITQGDEVIVPTFT
YVASVNTIVQCGALPVFAEIEGESLQVSVEDVKRKINKKTKAVMAVHIYGQACDIQSLRDLCDEHGLYLIEDCAEAIGTA
VNGKKVGTFGDVSTFSFFGNKTITSGEGGMVVSNSDIIIDKCLRLKNQGVVAGKRYWHDLVAYNYRMTNLCAAIGVAQLE
RVDKIIKRKRDIAEIYRSELAGLPMQVHKESNGTFHSYWLTSIILDQEFEVHRDGLMTFLENNDIESRPFFYPAHTLPMY
EHLAEKTAFPLSNSYSHRGINLPSWPGLCDDQVKEICNCIKNYFNCI
>Mature_367_residues
MIPVYEPSLDGNERKYLNDCIDSGWVSSRGKYIDRFETEFAEFLKVKHATTVSNGTVALHLAMSALGITQGDEVIVPTFT
YVASVNTIVQCGALPVFAEIEGESLQVSVEDVKRKINKKTKAVMAVHIYGQACDIQSLRDLCDEHGLYLIEDCAEAIGTA
VNGKKVGTFGDVSTFSFFGNKTITSGEGGMVVSNSDIIIDKCLRLKNQGVVAGKRYWHDLVAYNYRMTNLCAAIGVAQLE
RVDKIIKRKRDIAEIYRSELAGLPMQVHKESNGTFHSYWLTSIILDQEFEVHRDGLMTFLENNDIESRPFFYPAHTLPMY
EHLAEKTAFPLSNSYSHRGINLPSWPGLCDDQVKEICNCIKNYFNCI

Specific function: May be involved in the production of the exopolysaccharide (EPS) component of the extracellular matrix during biofilm formation. EPS is responsible for the adhesion of chains of cells into bundles [H]

COG id: COG0399

COG function: function code M; Predicted pyridoxal phosphate-dependent enzyme apparently involved in regulation of cell wall biogenesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the degT/dnrJ/eryC1 family [H]

Homologues:

Organism=Escherichia coli, GI145693159, Length=373, Percent_Identity=29.4906166219839, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI2367285, Length=385, Percent_Identity=26.4935064935065, Blast_Score=145, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000653
- InterPro:   IPR002129
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01041 DegT_DnrJ_EryC1 [H]

EC number: NA

Molecular weight: Translated: 41096; Mature: 41096

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPVYEPSLDGNERKYLNDCIDSGWVSSRGKYIDRFETEFAEFLKVKHATTVSNGTVALH
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCEEEEE
LAMSALGITQGDEVIVPTFTYVASVNTIVQCGALPVFAEIEGESLQVSVEDVKRKINKKT
EHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEHHHHHHHHHHHH
KAVMAVHIYGQACDIQSLRDLCDEHGLYLIEDCAEAIGTAVNGKKVGTFGDVSTFSFFGN
HEEEEEEEECCCCCHHHHHHHHHHCCCEEHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCC
KTITSGEGGMVVSNSDIIIDKCLRLKNQGVVAGKRYWHDLVAYNYRMTNLCAAIGVAQLE
CEEECCCCCEEEECCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVDKIIKRKRDIAEIYRSELAGLPMQVHKESNGTFHSYWLTSIILDQEFEVHRDGLMTFL
HHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHH
ENNDIESRPFFYPAHTLPMYEHLAEKTAFPLSNSYSHRGINLPSWPGLCDDQVKEICNCI
CCCCCCCCCEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
KNYFNCI
HHHHCCC
>Mature Secondary Structure
MIPVYEPSLDGNERKYLNDCIDSGWVSSRGKYIDRFETEFAEFLKVKHATTVSNGTVALH
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCEEEEE
LAMSALGITQGDEVIVPTFTYVASVNTIVQCGALPVFAEIEGESLQVSVEDVKRKINKKT
EHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEHHHHHHHHHHHH
KAVMAVHIYGQACDIQSLRDLCDEHGLYLIEDCAEAIGTAVNGKKVGTFGDVSTFSFFGN
HEEEEEEEECCCCCHHHHHHHHHHCCCEEHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCC
KTITSGEGGMVVSNSDIIIDKCLRLKNQGVVAGKRYWHDLVAYNYRMTNLCAAIGVAQLE
CEEECCCCCEEEECCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVDKIIKRKRDIAEIYRSELAGLPMQVHKESNGTFHSYWLTSIILDQEFEVHRDGLMTFL
HHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHH
ENNDIESRPFFYPAHTLPMYEHLAEKTAFPLSNSYSHRGINLPSWPGLCDDQVKEICNCI
CCCCCCCCCEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
KNYFNCI
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969506; 9384377 [H]