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The map label for this gene is rfaD

Identifier: 227080459

GI number: 227080459

Start: 231131

End: 232075

Strand: Reverse

Name: rfaD

Synonym: VCM66_0228

Alternate gene names: 227080459

Gene position: 232075-231131 (Counterclockwise)

Preceding gene: 227080474

Following gene: 227080458

Centisome position: 8.02

GC content: 46.14

Gene sequence:

>945_bases
ATGATTATCGTAACTGGCGGCGCTGGCATGATTGGCAGCAACATTATCAAAGCGCTTAATGAGCGCGGTATCACAGACAT
TCTGGTCGTCGATCATTTGAAAAATGGTCGTAAGTTCAAAAATCTGGTTGACCTACAGATCGCTGACTACATGGATCGAG
ATGACTTCCTAGCTCAGATCATGGCCGGTGATGATTTTGGGTTCATTGACGCCATTTTTCATGAGGGAGCTTGCTCTGCG
ACCACAGAGTGGGATGGCAAATATGTCATGCTCAACAACTATGAATACTCTAAAGAGCTGCTGCACTACTGTTTAGATCG
TGAAATACCCTTCCTGTATGCCTCTTCAGCAGCCACTTATGGTGAAACCGATACCTTTATTGAAGAACCGCAATACGAAG
GTGCACTCAACGTTTACGGCTATTCGAAGCAGCAATTTGATAACTATGTACGCCGCCTATGGCTAGATGCAAAACAACAC
GACGAAACCCTATCGCAAATAACGGGATTTCGTTACTTCAACGTATACGGCCCTCGTGAGCAGCATAAAGGCTCGATGGC
TTCGGTTGCCTTTCATTTGAACAATCAAATGAACGCGGGTGAAAATCCAAAATTGTTTGCTGGCAGCGAGAACTTCAAAC
GTGATTTCGTTTATGTCGGCGATGTCGCTGCGGTCAATTTGTGGTTTTTAGATCATGGCGTATCCGGCATATTTAACTGT
GGTACTGGTAAAGCGGAATCGTTCAACGAAGTGGCGAAAGCGGTGATCGCATTCCATGGTCGCGGCGAAGTCGAAACCAT
TCCATTCCCAGACCATCTTAAAGGTGCTTACCAAGAGTTCACTGAGGCTGACCTTACCAAACTTCGCGCTGCTGGCTGTG
ATGTACAGTTCAAGAGCGTGGCTGAAGGTGTCGCTGAATATATGGCGCTGATTAATCGTAAGTAA

Upstream 100 bases:

>100_bases
CAGGGCGAGCCCTCAAAAGGCGGAGCTTAGATTTTGTGATTACAGTAAGAAAATGACAGGCTATAATGCGTGCAACTGTT
CGCATCTATAGGAATTCATA

Downstream 100 bases:

>100_bases
GGGAAACCCTTAAACCCTCCTAACCTCCCCCTTAAAGGGGGAGGAACAAAGGGATTGCCTCTTTGTAGAGGGGATAGATG
AATTACCTTCCAAAAGAGAA

Product: ADP-L-glycero-D-mannoheptose-6-epimerase

Products: NA

Alternate protein names: ADP-L-glycero-beta-D-manno-heptose-6-epimerase; ADP-glyceromanno-heptose 6-epimerase; ADP-hep 6-epimerase; AGME

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MIIVTGGAGMIGSNIIKALNERGITDILVVDHLKNGRKFKNLVDLQIADYMDRDDFLAQIMAGDDFGFIDAIFHEGACSA
TTEWDGKYVMLNNYEYSKELLHYCLDREIPFLYASSAATYGETDTFIEEPQYEGALNVYGYSKQQFDNYVRRLWLDAKQH
DETLSQITGFRYFNVYGPREQHKGSMASVAFHLNNQMNAGENPKLFAGSENFKRDFVYVGDVAAVNLWFLDHGVSGIFNC
GTGKAESFNEVAKAVIAFHGRGEVETIPFPDHLKGAYQEFTEADLTKLRAAGCDVQFKSVAEGVAEYMALINRK

Sequences:

>Translated_314_residues
MIIVTGGAGMIGSNIIKALNERGITDILVVDHLKNGRKFKNLVDLQIADYMDRDDFLAQIMAGDDFGFIDAIFHEGACSA
TTEWDGKYVMLNNYEYSKELLHYCLDREIPFLYASSAATYGETDTFIEEPQYEGALNVYGYSKQQFDNYVRRLWLDAKQH
DETLSQITGFRYFNVYGPREQHKGSMASVAFHLNNQMNAGENPKLFAGSENFKRDFVYVGDVAAVNLWFLDHGVSGIFNC
GTGKAESFNEVAKAVIAFHGRGEVETIPFPDHLKGAYQEFTEADLTKLRAAGCDVQFKSVAEGVAEYMALINRK
>Mature_314_residues
MIIVTGGAGMIGSNIIKALNERGITDILVVDHLKNGRKFKNLVDLQIADYMDRDDFLAQIMAGDDFGFIDAIFHEGACSA
TTEWDGKYVMLNNYEYSKELLHYCLDREIPFLYASSAATYGETDTFIEEPQYEGALNVYGYSKQQFDNYVRRLWLDAKQH
DETLSQITGFRYFNVYGPREQHKGSMASVAFHLNNQMNAGENPKLFAGSENFKRDFVYVGDVAAVNLWFLDHGVSGIFNC
GTGKAESFNEVAKAVIAFHGRGEVETIPFPDHLKGAYQEFTEADLTKLRAAGCDVQFKSVAEGVAEYMALINRK

Specific function: Catalyzes the interconversion between ADP-D-glycero- beta-D-manno-heptose and ADP-L-glycero-beta-D-manno-heptose via an epimerization at carbon 6 of the heptose

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. HldD subfamily

Homologues:

Organism=Escherichia coli, GI1790049, Length=314, Percent_Identity=74.2038216560509, Blast_Score=476, Evalue=1e-136,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): HLDD_VIBC3 (A5F3Z4)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F3Z4
- SMR:   A5F3Z4
- STRING:   A5F3Z4
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A2620
- eggNOG:   COG0451
- HOGENOM:   HBG755066
- OMA:   GFIGSAM
- ProtClustDB:   PRK11150
- BioCyc:   VCHO345073:VC0395_A2620-MONOMER
- HAMAP:   MF_01601
- InterPro:   IPR001509
- InterPro:   IPR011912
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR10366:SF29
- TIGRFAMs:   TIGR02197

Pfam domain/function: PF01370 Epimerase

EC number: =5.1.3.20

Molecular weight: Translated: 35245; Mature: 35245

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: ACT_SITE 139-139 ACT_SITE 183-183 BINDING 38-38 BINDING 53-53 BINDING 92-92 BINDING 143-143 BINDING 174-174 BINDING 175-175 BINDING 183-183 BINDING 185-185 BINDING 192-192 BINDING 214-214 BINDING 277-277

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIVTGGAGMIGSNIIKALNERGITDILVVDHLKNGRKFKNLVDLQIADYMDRDDFLAQI
CEEEECCCCHHHHHHHHHHHHCCCEEEEEEHHHCCCHHHHHHHHHHHHHHCCHHHHHHHH
MAGDDFGFIDAIFHEGACSATTEWDGKYVMLNNYEYSKELLHYCLDREIPFLYASSAATY
HCCCCCCHHHHHHHCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHCCCCCEEEECCCCCC
GETDTFIEEPQYEGALNVYGYSKQQFDNYVRRLWLDAKQHDETLSQITGFRYFNVYGPRE
CCCCCHHCCCCCCCCEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEECCCHH
QHKGSMASVAFHLNNQMNAGENPKLFAGSENFKRDFVYVGDVAAVNLWFLDHGVSGIFNC
HHCCCCEEEEEEECCCCCCCCCCCEEECCCCCCCCEEEECCEEEEEEEEECCCCCCEEEC
GTGKAESFNEVAKAVIAFHGRGEVETIPFPDHLKGAYQEFTEADLTKLRAAGCDVQFKSV
CCCCCCHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
AEGVAEYMALINRK
HHHHHHHHHHHCCC
>Mature Secondary Structure
MIIVTGGAGMIGSNIIKALNERGITDILVVDHLKNGRKFKNLVDLQIADYMDRDDFLAQI
CEEEECCCCHHHHHHHHHHHHCCCEEEEEEHHHCCCHHHHHHHHHHHHHHCCHHHHHHHH
MAGDDFGFIDAIFHEGACSATTEWDGKYVMLNNYEYSKELLHYCLDREIPFLYASSAATY
HCCCCCCHHHHHHHCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHCCCCCEEEECCCCCC
GETDTFIEEPQYEGALNVYGYSKQQFDNYVRRLWLDAKQHDETLSQITGFRYFNVYGPRE
CCCCCHHCCCCCCCCEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEECCCHH
QHKGSMASVAFHLNNQMNAGENPKLFAGSENFKRDFVYVGDVAAVNLWFLDHGVSGIFNC
HHCCCCEEEEEEECCCCCCCCCCCEEECCCCCCCCEEEECCEEEEEEEEECCCCCCEEEC
GTGKAESFNEVAKAVIAFHGRGEVETIPFPDHLKGAYQEFTEADLTKLRAAGCDVQFKSV
CCCCCCHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
AEGVAEYMALINRK
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA