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The map label for this gene is uvrD [H]

Identifier: 227080412

GI number: 227080412

Start: 180197

End: 182368

Strand: Direct

Name: uvrD [H]

Synonym: VCM66_0178

Alternate gene names: 227080412

Gene position: 180197-182368 (Clockwise)

Preceding gene: 227080411

Following gene: 227080417

Centisome position: 6.23

GC content: 52.72

Gene sequence:

>2172_bases
ATGATCGATCCCTCTCTATTGCTCGACGGTCTCAACGATAAACAACGCGAAGCGGTGGCAGCGCCACTGGAAAACTTACT
GATTTTGGCCGGTGCCGGCAGTGGTAAAACCCGCGTACTGGTGCACCGAATTGCGTGGCTGATGTCGGTGGAAGAAGCCT
CACCGTTTTCGGTGATGGCGGTGACCTTTACCAACAAAGCTGCAGCCGAAATGCGTGGCCGAATTGAAGAGCTGATGCAT
GGCACCGCCTCTGGCATGTGGTGTGGGACTTTCCACGGTATTTGCCACCGTATCTTGCGCGCTCACTATCTGGACGCTAA
GTTGCTGGAAGATTTCCAGATTATCGACAGTGACGATCAGCAGCGTCTGCTCAAACGCCTCATCAAAGCGCACAATCTGG
ATGATAAACAGTGGCCGGCACGCCAAGTCGCGTGGTGGATCAACAATCAAAAAGATGAAGGGTTGCGCCCGGCGCATATC
AACGCCTTTGATCCGGTTACTCAAACTTATCTCAAGCTGTACACCGCTTACCAAGAAGCGTGCGATCGCGCCGGCTTGGT
CGATTTCGCCGAGATTTTGCTGCGCGCCCTAGAGCTTCTGCGTGGTAATCAGCACATTCGCGAGCATTATCAGGCGCGCT
TTAAGCACATTTTGGTCGATGAATTCCAAGACACCAACGCGATTCAATACGCGTGGCTGCGCATGATGGCGGGTGCACAG
TCGAACGTGATGATTGTCGGTGATGATGACCAATCGATATACGGCTGGCGTGGCGCGCGAGTGGAAAACATCGAAAAATT
TACCCGCGAATTTCCGAGCGTGAATACCATTCGACTGGAGCAAAACTACCGCTCCACTAAGACCATTTTGGAAGCCTCAA
ATACACTGATCGCCAATAACAGCGAGCGGATGGGCAAGCAGCTATGGACCGAAGGTTTGGTTGGCGAACCCATTTCGGTG
TATTCGGCGTATAACGAGCTCGATGAAGCGCGCTTTGTGGTCAGCAAGATCAAAGGTTGGCAGGAGCAAGGCGGCACACT
GACCGATTGCGCGATCTTGTATCGCAACAACGCCCAGTCACGGGTATTGGAAGAGGCTTTATTGCAAGCGAGCTTGGCCT
ACCGCATTTATGGCGGGATGCGTTTTTTCGAACGCCAAGAGATCAAAGATGCGCTGAGTTATCTGCGTTTGATTAATAAC
CGCAATGACGACACCGCCTTTGAGCGAGTGATTAATACGCCGCCGCGCGGTTTGGGTGATAAAACCCTAGAGACCATTCG
CTTTGCCGCGCGCGATCGTGGCTGCACCTTGTGGGATGCCAGTGTCGGTCTGCTTAATGACCAAGTCTTAACTGGGCGTG
CAGCTAGCGCGCTGAGTCGCTTTGTTGAACTGATCAATGCTTTGGAAGAGGAAGGCATCGACATGCCGCTGCATGTGCTC
ACGGACCACGCGGTAAAAACCTCTGGTCTGCTGGAGATGTATCAGCAAGAAAAAGGCGAAAAATCCAAAGCGCGGATTGA
AAACTTGGAAGAGCTGGTCACGGCGACGCGCCAGTTTGAAAAGCCAGAAGAAGCGCAGGATATGACCATGCTGACCGCGT
TTTTGACCCATGCGGCGCTTGAAGCGGGTGAAGGCCAAGCTGATGAGCATGATGATGCGGTACAGCTGATGACTTTACAC
AGTGCCAAAGGATTAGAGTTCCCGCTGGTGTTTATGGTCGGTGTGGAAGAGGGCATGTTCCCGAGCCAAATGTCTGCCGA
AGAGGCCGGGCGTCTCGAAGAGGAGCGTCGCCTGTGTTACGTCGGTATGACCCGCGCGATGCAAAAGCTCTACATCACCT
ACGCGGAAATGCGCCGCTTGTACGGGCAAGATAAGTATCACAAACCCTCACGCTTTATTCGTGAACTGCCAGAAGGCTGT
TTGGATGAAGTACGCATGAAGGCGCAAGTCAGCCGCCCAACCAGCACAGGACGCTTTAGCCAAACCGTAGTCAAAGAAAG
CTTTAACGAAACCGGTTTTAATTTGGGCTCACGAGTCCGTCATCCGAAATTTGGTGAAGGCACCATCATTAACTTTGAAG
GCAGTGGCCCGCAAAGCCGAGTGCAAGTGGCGTTTAATGGCGAAGGCATTAAGTGGTTAGTGACGGCTTACGCGAGATTA
GAGAAGGTGTAG

Upstream 100 bases:

>100_bases
TTTTTCCGGTCACCGCTGCGCCCATTCAAGACGTTTGGGTATCTGCGACACTTGGGGTTATAATCGCCGCCATTATGTAT
AAATAACCAGTGGCGAAGTC

Downstream 100 bases:

>100_bases
ATAGAATAGTGCGGCGACGTGGCTCCCCCCTTTTAGGGGGAGGTCGGGAAGAAATTGCCAAATTGCCCCTAGATACAAAG
TAACGATTAACGTTTCTGTT

Product: DNA-dependent helicase II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 723; Mature: 723

Protein sequence:

>723_residues
MIDPSLLLDGLNDKQREAVAAPLENLLILAGAGSGKTRVLVHRIAWLMSVEEASPFSVMAVTFTNKAAAEMRGRIEELMH
GTASGMWCGTFHGICHRILRAHYLDAKLLEDFQIIDSDDQQRLLKRLIKAHNLDDKQWPARQVAWWINNQKDEGLRPAHI
NAFDPVTQTYLKLYTAYQEACDRAGLVDFAEILLRALELLRGNQHIREHYQARFKHILVDEFQDTNAIQYAWLRMMAGAQ
SNVMIVGDDDQSIYGWRGARVENIEKFTREFPSVNTIRLEQNYRSTKTILEASNTLIANNSERMGKQLWTEGLVGEPISV
YSAYNELDEARFVVSKIKGWQEQGGTLTDCAILYRNNAQSRVLEEALLQASLAYRIYGGMRFFERQEIKDALSYLRLINN
RNDDTAFERVINTPPRGLGDKTLETIRFAARDRGCTLWDASVGLLNDQVLTGRAASALSRFVELINALEEEGIDMPLHVL
TDHAVKTSGLLEMYQQEKGEKSKARIENLEELVTATRQFEKPEEAQDMTMLTAFLTHAALEAGEGQADEHDDAVQLMTLH
SAKGLEFPLVFMVGVEEGMFPSQMSAEEAGRLEEERRLCYVGMTRAMQKLYITYAEMRRLYGQDKYHKPSRFIRELPEGC
LDEVRMKAQVSRPTSTGRFSQTVVKESFNETGFNLGSRVRHPKFGEGTIINFEGSGPQSRVQVAFNGEGIKWLVTAYARL
EKV

Sequences:

>Translated_723_residues
MIDPSLLLDGLNDKQREAVAAPLENLLILAGAGSGKTRVLVHRIAWLMSVEEASPFSVMAVTFTNKAAAEMRGRIEELMH
GTASGMWCGTFHGICHRILRAHYLDAKLLEDFQIIDSDDQQRLLKRLIKAHNLDDKQWPARQVAWWINNQKDEGLRPAHI
NAFDPVTQTYLKLYTAYQEACDRAGLVDFAEILLRALELLRGNQHIREHYQARFKHILVDEFQDTNAIQYAWLRMMAGAQ
SNVMIVGDDDQSIYGWRGARVENIEKFTREFPSVNTIRLEQNYRSTKTILEASNTLIANNSERMGKQLWTEGLVGEPISV
YSAYNELDEARFVVSKIKGWQEQGGTLTDCAILYRNNAQSRVLEEALLQASLAYRIYGGMRFFERQEIKDALSYLRLINN
RNDDTAFERVINTPPRGLGDKTLETIRFAARDRGCTLWDASVGLLNDQVLTGRAASALSRFVELINALEEEGIDMPLHVL
TDHAVKTSGLLEMYQQEKGEKSKARIENLEELVTATRQFEKPEEAQDMTMLTAFLTHAALEAGEGQADEHDDAVQLMTLH
SAKGLEFPLVFMVGVEEGMFPSQMSAEEAGRLEEERRLCYVGMTRAMQKLYITYAEMRRLYGQDKYHKPSRFIRELPEGC
LDEVRMKAQVSRPTSTGRFSQTVVKESFNETGFNLGSRVRHPKFGEGTIINFEGSGPQSRVQVAFNGEGIKWLVTAYARL
EKV
>Mature_723_residues
MIDPSLLLDGLNDKQREAVAAPLENLLILAGAGSGKTRVLVHRIAWLMSVEEASPFSVMAVTFTNKAAAEMRGRIEELMH
GTASGMWCGTFHGICHRILRAHYLDAKLLEDFQIIDSDDQQRLLKRLIKAHNLDDKQWPARQVAWWINNQKDEGLRPAHI
NAFDPVTQTYLKLYTAYQEACDRAGLVDFAEILLRALELLRGNQHIREHYQARFKHILVDEFQDTNAIQYAWLRMMAGAQ
SNVMIVGDDDQSIYGWRGARVENIEKFTREFPSVNTIRLEQNYRSTKTILEASNTLIANNSERMGKQLWTEGLVGEPISV
YSAYNELDEARFVVSKIKGWQEQGGTLTDCAILYRNNAQSRVLEEALLQASLAYRIYGGMRFFERQEIKDALSYLRLINN
RNDDTAFERVINTPPRGLGDKTLETIRFAARDRGCTLWDASVGLLNDQVLTGRAASALSRFVELINALEEEGIDMPLHVL
TDHAVKTSGLLEMYQQEKGEKSKARIENLEELVTATRQFEKPEEAQDMTMLTAFLTHAALEAGEGQADEHDDAVQLMTLH
SAKGLEFPLVFMVGVEEGMFPSQMSAEEAGRLEEERRLCYVGMTRAMQKLYITYAEMRRLYGQDKYHKPSRFIRELPEGC
LDEVRMKAQVSRPTSTGRFSQTVVKESFNETGFNLGSRVRHPKFGEGTIINFEGSGPQSRVQVAFNGEGIKWLVTAYARL
EKV

Specific function: Has both ATPase and helicase activities. Unwinds DNA duplexes with 3' to 5' polarity with respect to the bound strand and initiates unwinding most effectively when a single-stranded region is present. Involved in the post-incision events of nucleotide exc

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=723, Percent_Identity=71.0926694329184, Blast_Score=1066, Evalue=0.0,
Organism=Escherichia coli, GI48994965, Length=645, Percent_Identity=39.2248062015504, Blast_Score=399, Evalue=1e-112,
Organism=Escherichia coli, GI1787196, Length=338, Percent_Identity=28.1065088757396, Blast_Score=104, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6322369, Length=739, Percent_Identity=28.4167794316644, Blast_Score=197, Evalue=6e-51,
Organism=Saccharomyces cerevisiae, GI6324477, Length=681, Percent_Identity=21.8795888399413, Blast_Score=118, Evalue=3e-27,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005753
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 82024; Mature: 82024

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDPSLLLDGLNDKQREAVAAPLENLLILAGAGSGKTRVLVHRIAWLMSVEEASPFSVMA
CCCHHHHHCCCCHHHHHHHHHCHHCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEE
VTFTNKAAAEMRGRIEELMHGTASGMWCGTFHGICHRILRAHYLDAKLLEDFQIIDSDDQ
EEECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
QRLLKRLIKAHNLDDKQWPARQVAWWINNQKDEGLRPAHINAFDPVTQTYLKLYTAYQEA
HHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
CDRAGLVDFAEILLRALELLRGNQHIREHYQARFKHILVDEFQDTNAIQYAWLRMMAGAQ
HHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCC
SNVMIVGDDDQSIYGWRGARVENIEKFTREFPSVNTIRLEQNYRSTKTILEASNTLIANN
CCEEEEECCCCCEECCCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCEEECC
SERMGKQLWTEGLVGEPISVYSAYNELDEARFVVSKIKGWQEQGGTLTDCAILYRNNAQS
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHCCCCEEHHHEEECCCHHH
RVLEEALLQASLAYRIYGGMRFFERQEIKDALSYLRLINNRNDDTAFERVINTPPRGLGD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCH
KTLETIRFAARDRGCTLWDASVGLLNDQVLTGRAASALSRFVELINALEEEGIDMPLHVL
HHHHHHHHHHCCCCCEEEECCCCHHCCHHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHHH
TDHAVKTSGLLEMYQQEKGEKSKARIENLEELVTATRQFEKPEEAQDMTMLTAFLTHAAL
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
EAGEGQADEHDDAVQLMTLHSAKGLEFPLVFMVGVEEGMFPSQMSAEEAGRLEEERRLCY
HCCCCCCCCCCCHHHHHEECCCCCCCCCEEEEEECCCCCCCCCCCHHHHHCHHHHHHHHH
VGMTRAMQKLYITYAEMRRLYGQDKYHKPSRFIRELPEGCLDEVRMKAQVSRPTSTGRFS
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
QTVVKESFNETGFNLGSRVRHPKFGEGTIINFEGSGPQSRVQVAFNGEGIKWLVTAYARL
HHHHHHHHHHCCCCCHHHCCCCCCCCCCEEEECCCCCCCEEEEEECCCCHHHHHHHHHHH
EKV
HCC
>Mature Secondary Structure
MIDPSLLLDGLNDKQREAVAAPLENLLILAGAGSGKTRVLVHRIAWLMSVEEASPFSVMA
CCCHHHHHCCCCHHHHHHHHHCHHCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEE
VTFTNKAAAEMRGRIEELMHGTASGMWCGTFHGICHRILRAHYLDAKLLEDFQIIDSDDQ
EEECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
QRLLKRLIKAHNLDDKQWPARQVAWWINNQKDEGLRPAHINAFDPVTQTYLKLYTAYQEA
HHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
CDRAGLVDFAEILLRALELLRGNQHIREHYQARFKHILVDEFQDTNAIQYAWLRMMAGAQ
HHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCC
SNVMIVGDDDQSIYGWRGARVENIEKFTREFPSVNTIRLEQNYRSTKTILEASNTLIANN
CCEEEEECCCCCEECCCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCEEECC
SERMGKQLWTEGLVGEPISVYSAYNELDEARFVVSKIKGWQEQGGTLTDCAILYRNNAQS
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHCCCCEEHHHEEECCCHHH
RVLEEALLQASLAYRIYGGMRFFERQEIKDALSYLRLINNRNDDTAFERVINTPPRGLGD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCH
KTLETIRFAARDRGCTLWDASVGLLNDQVLTGRAASALSRFVELINALEEEGIDMPLHVL
HHHHHHHHHHCCCCCEEEECCCCHHCCHHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHHH
TDHAVKTSGLLEMYQQEKGEKSKARIENLEELVTATRQFEKPEEAQDMTMLTAFLTHAAL
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
EAGEGQADEHDDAVQLMTLHSAKGLEFPLVFMVGVEEGMFPSQMSAEEAGRLEEERRLCY
HCCCCCCCCCCCHHHHHEECCCCCCCCCEEEEEECCCCCCCCCCCHHHHHCHHHHHHHHH
VGMTRAMQKLYITYAEMRRLYGQDKYHKPSRFIRELPEGCLDEVRMKAQVSRPTSTGRFS
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
QTVVKESFNETGFNLGSRVRHPKFGEGTIINFEGSGPQSRVQVAFNGEGIKWLVTAYARL
HHHHHHHHHHCCCCCHHHCCCCCCCCCCEEEECCCCCCCEEEEEECCCCHHHHHHHHHHH
EKV
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6379604; 2943729; 1379743; 9278503; 6324092; 2254268; 8419285 [H]