Click here to switch to the map view.

The map label for this gene is plsB [H]

Identifier: 227080329

GI number: 227080329

Start: 90918

End: 93353

Strand: Direct

Name: plsB [H]

Synonym: VCM66_0093

Alternate gene names: 227080329

Gene position: 90918-93353 (Clockwise)

Preceding gene: 227080325

Following gene: 227080333

Centisome position: 3.14

GC content: 51.72

Gene sequence:

>2436_bases
TTGAACTCTATGTCTTCAGGACACCTATTATCCCGTTCACTACTGAAGTTGCCGATGTCGGTGCTGGTGAAAGGGACGGC
GATTCCGTCTAATCCGATCCAAGATCTGGATATCGATACTCATAAACCGGTGATTTACGCCTTACCGTTTCGCTCCAACG
TCGACTTGCTGACGTTGCAAACACATGCCAAAGAAGCTGGGCTGCCTGACCCTTTAGAGCCTTTGATGCTGAATGGCAAA
GCTTTCCAGCGCTATGTGTTTATTGCTTCGCGCCCAACGCTGCTGAGTAGCGATCAACATGTGCCGAGTGACTCTATTGC
GCTGTTTTCTGAGCTGCTGACCGAACATAAGCTCGACAGTGAGTTGGATGTGCAGGTGATTCCAGCAACGGTTTTGTGGG
GACGTAAACCGGGCAAAGAAGGGCAAGAAAGACCCTATTTGCAAGCGTTGAATGGTCCGGAGAAAGCGCTGGCCGTTTTG
GCTTCTGGCCGTGACTGCTTGGTGCGTTTTAGTCCTGTGGTGTCGATGCGTTACATGGCGGACACCCACGGCACCGATGC
CTCGATTGCCCATAAATTGGCTCGTGTAGCACGCATTCACTTCTCTCGGCAAAAACTGGCGGCGTCAGGCCCTAATTTGC
CGCAGCGAGCACAACTGTTTGCCCGCTTGATGAATTCTCCTGCGATTGAAAAAGCGATTGCGGACGAAGCGAAATCCAAA
CAGATCCCACTTGAGAAAGCGCGCAAAGAAGCGCACGACATTCTGGATGAGATCGCGGCGGATTTTTCCTATTCGCTCGT
GAAAAAAGGCGATCGCATTTTAGGTTGGTTGTGGAACCGCATCTACCAAGGTTTGAACATCAATAACGCCGCAACGGTGC
GTCGCTTGGCGCAAGATGGCCATGAGATTGTCTACGTGCCTTGTCACCGTAGCCATATGGATTACTTGCTGCTTTCTTAT
GTGCTTTATCATGAAGGCATGGTGCCTCCGCACATTGCCGCGGGGATTAACTTGAACTTTTTCCCGGCGGGGCCGATTTT
CCGCCGCGGCGGCGCGTTTTTCATTCGTCGTAGCTTTAAAGGTGCGCCACTTTATTCAACGATTTTCCGTGAATACCTCG
CGGAGCTGTTTGCGAAAGGCTATTCGGTGGAATACTTCAGCGAAGGTGGCCGCTCGCGTACAGGTCGCTTGTTGCCAGCG
AAAACGGGCATGTTGGCGATGACCATTCAAGCCATGTTGCGTGGCCTGAATCGTCCCGTCACGTTAGTGCCTGTCTACAT
CGGCTATGAGCATGTGATGGAAGTGGGTACTTACGCCAAAGAGCTACGCGGCAAACGTAAAGAGAAAGAAAATGCCGGTT
TAGTGCTGCGCACGCTGCGTAAGCTGCGCAATTTTGGCCAAGGTTACGTGAACTTCGGTGAGCCTATCCCACTCAACCAG
TTTTTGAATGAAACCGTACCGCAATGGACGCAAGATATTGACCCGATGGGCGAGAGCAAGCCGCAATGGATGACTCCAAC
GGTGAATAAACTCGCGAACCGTATGATGACGCACATCAACGATGCGGCAGCGGTGAATGCGATGACCTTGTGTGCGACGG
CGCTGCTTGCTTCGCGTCAGCGTGCCTTGGCGCGTGACAACCTGATTAAGCAGGTGGATTGTTACTTATCGCTGCTACGC
AATGTGCCGTATTCAGCCACATCAACGCTGCCGAGTGAGAGTGCAGAGAAGCTGGTAGAACATGCGGAGTCGTTGGATAA
GTTTGTGGTGGAAACCGATACCATGGGCGACATTATTTCGCTGGATCGTAACCAATCGATCCTGATGACTTATTATCGCA
ACAACATTATCCACCTGTTGGCTCTGCCATCGCTGATCGCTCAGTTGTTGATTCGTCAGCAAAGCGTATCGCTGGAAAAG
GTGCAAGCGACCGTTGCACAAATCTATCCTTTCCTGAAACAAGAGCTGTTTTTGCGTTTTGAGGCAGAAGAACTCAACGA
TCTGGTACTGCGTTATGTGGCGGAGCTAGCACGCCAAGGTTTGGTCACGGTGGAAGGGAAAACCGTCACCCTGAATCAAG
CGCAAACTCAAGTGCTGATGCTGCTGGGGCGCATTATCTCCGAAACGCTGCAGCGTTATGCGATTGCGCTGAACCTGCTG
GTGAGCTGCCCACATTTGGGTAAAGCTGAGCTGGAAGAGAAGAGTCAGGAAGTGGCGCAGCGTCTTGGCCGTTTGCATGG
CATTAATGCGCCTGAGTTCTTTGATAAAGGGGTATTTGCTTCGCTGTTCGTGACGCTACAAGAGCAGGGATATCTCGATG
ATCAAGGCCGCTGCGTATTAGAAACAGCGAAGCCGCTCTCTCGCCAGCTTTATGCGCTGATCTACCCAGAAGTGCGCATG
ACGATTCAAGAAAGCTTGTGTCAGGTTGACGCGTAA

Upstream 100 bases:

>100_bases
TATTCTTGGCGCGCAAAAAACGATACCGCACGATGGTGAGCTCTCGCGTTGGCCGTGAAAGCAGACGTACTGTCGCGCTT
ATTTCAAGAATCTTGAGGCT

Downstream 100 bases:

>100_bases
CGATTGTGTTCTAGCCAATCGAACCGAATGAAAAAGGCATCCTGCGGATGCCTTTTCTTTTGCCTTAAGCTGGGATTACC
AAACGCTGAGCAGAATGCCT

Product: glycerol-3-phosphate acyltransferase

Products: NA

Alternate protein names: GPAT [H]

Number of amino acids: Translated: 811; Mature: 811

Protein sequence:

>811_residues
MNSMSSGHLLSRSLLKLPMSVLVKGTAIPSNPIQDLDIDTHKPVIYALPFRSNVDLLTLQTHAKEAGLPDPLEPLMLNGK
AFQRYVFIASRPTLLSSDQHVPSDSIALFSELLTEHKLDSELDVQVIPATVLWGRKPGKEGQERPYLQALNGPEKALAVL
ASGRDCLVRFSPVVSMRYMADTHGTDASIAHKLARVARIHFSRQKLAASGPNLPQRAQLFARLMNSPAIEKAIADEAKSK
QIPLEKARKEAHDILDEIAADFSYSLVKKGDRILGWLWNRIYQGLNINNAATVRRLAQDGHEIVYVPCHRSHMDYLLLSY
VLYHEGMVPPHIAAGINLNFFPAGPIFRRGGAFFIRRSFKGAPLYSTIFREYLAELFAKGYSVEYFSEGGRSRTGRLLPA
KTGMLAMTIQAMLRGLNRPVTLVPVYIGYEHVMEVGTYAKELRGKRKEKENAGLVLRTLRKLRNFGQGYVNFGEPIPLNQ
FLNETVPQWTQDIDPMGESKPQWMTPTVNKLANRMMTHINDAAAVNAMTLCATALLASRQRALARDNLIKQVDCYLSLLR
NVPYSATSTLPSESAEKLVEHAESLDKFVVETDTMGDIISLDRNQSILMTYYRNNIIHLLALPSLIAQLLIRQQSVSLEK
VQATVAQIYPFLKQELFLRFEAEELNDLVLRYVAELARQGLVTVEGKTVTLNQAQTQVLMLLGRIISETLQRYAIALNLL
VSCPHLGKAELEEKSQEVAQRLGRLHGINAPEFFDKGVFASLFVTLQEQGYLDDQGRCVLETAKPLSRQLYALIYPEVRM
TIQESLCQVDA

Sequences:

>Translated_811_residues
MNSMSSGHLLSRSLLKLPMSVLVKGTAIPSNPIQDLDIDTHKPVIYALPFRSNVDLLTLQTHAKEAGLPDPLEPLMLNGK
AFQRYVFIASRPTLLSSDQHVPSDSIALFSELLTEHKLDSELDVQVIPATVLWGRKPGKEGQERPYLQALNGPEKALAVL
ASGRDCLVRFSPVVSMRYMADTHGTDASIAHKLARVARIHFSRQKLAASGPNLPQRAQLFARLMNSPAIEKAIADEAKSK
QIPLEKARKEAHDILDEIAADFSYSLVKKGDRILGWLWNRIYQGLNINNAATVRRLAQDGHEIVYVPCHRSHMDYLLLSY
VLYHEGMVPPHIAAGINLNFFPAGPIFRRGGAFFIRRSFKGAPLYSTIFREYLAELFAKGYSVEYFSEGGRSRTGRLLPA
KTGMLAMTIQAMLRGLNRPVTLVPVYIGYEHVMEVGTYAKELRGKRKEKENAGLVLRTLRKLRNFGQGYVNFGEPIPLNQ
FLNETVPQWTQDIDPMGESKPQWMTPTVNKLANRMMTHINDAAAVNAMTLCATALLASRQRALARDNLIKQVDCYLSLLR
NVPYSATSTLPSESAEKLVEHAESLDKFVVETDTMGDIISLDRNQSILMTYYRNNIIHLLALPSLIAQLLIRQQSVSLEK
VQATVAQIYPFLKQELFLRFEAEELNDLVLRYVAELARQGLVTVEGKTVTLNQAQTQVLMLLGRIISETLQRYAIALNLL
VSCPHLGKAELEEKSQEVAQRLGRLHGINAPEFFDKGVFASLFVTLQEQGYLDDQGRCVLETAKPLSRQLYALIYPEVRM
TIQESLCQVDA
>Mature_811_residues
MNSMSSGHLLSRSLLKLPMSVLVKGTAIPSNPIQDLDIDTHKPVIYALPFRSNVDLLTLQTHAKEAGLPDPLEPLMLNGK
AFQRYVFIASRPTLLSSDQHVPSDSIALFSELLTEHKLDSELDVQVIPATVLWGRKPGKEGQERPYLQALNGPEKALAVL
ASGRDCLVRFSPVVSMRYMADTHGTDASIAHKLARVARIHFSRQKLAASGPNLPQRAQLFARLMNSPAIEKAIADEAKSK
QIPLEKARKEAHDILDEIAADFSYSLVKKGDRILGWLWNRIYQGLNINNAATVRRLAQDGHEIVYVPCHRSHMDYLLLSY
VLYHEGMVPPHIAAGINLNFFPAGPIFRRGGAFFIRRSFKGAPLYSTIFREYLAELFAKGYSVEYFSEGGRSRTGRLLPA
KTGMLAMTIQAMLRGLNRPVTLVPVYIGYEHVMEVGTYAKELRGKRKEKENAGLVLRTLRKLRNFGQGYVNFGEPIPLNQ
FLNETVPQWTQDIDPMGESKPQWMTPTVNKLANRMMTHINDAAAVNAMTLCATALLASRQRALARDNLIKQVDCYLSLLR
NVPYSATSTLPSESAEKLVEHAESLDKFVVETDTMGDIISLDRNQSILMTYYRNNIIHLLALPSLIAQLLIRQQSVSLEK
VQATVAQIYPFLKQELFLRFEAEELNDLVLRYVAELARQGLVTVEGKTVTLNQAQTQVLMLLGRIISETLQRYAIALNLL
VSCPHLGKAELEEKSQEVAQRLGRLHGINAPEFFDKGVFASLFVTLQEQGYLDDQGRCVLETAKPLSRQLYALIYPEVRM
TIQESLCQVDA

Specific function: De novo phospholipid biosynthesis; first step. [C]

COG id: COG2937

COG function: function code I; Glycerol-3-phosphate O-acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GPAT/DAPAT family [H]

Homologues:

Organism=Homo sapiens, GI7657134, Length=526, Percent_Identity=25.2851711026616, Blast_Score=153, Evalue=7e-37,
Organism=Homo sapiens, GI190358539, Length=282, Percent_Identity=29.4326241134752, Blast_Score=124, Evalue=4e-28,
Organism=Escherichia coli, GI87082362, Length=804, Percent_Identity=56.9651741293532, Blast_Score=926, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25147672, Length=350, Percent_Identity=28.8571428571429, Blast_Score=152, Evalue=7e-37,
Organism=Caenorhabditis elegans, GI71988723, Length=474, Percent_Identity=25.7383966244726, Blast_Score=135, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI71988728, Length=414, Percent_Identity=26.8115942028986, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI17864692, Length=391, Percent_Identity=30.4347826086957, Blast_Score=161, Evalue=1e-39,
Organism=Drosophila melanogaster, GI21357731, Length=319, Percent_Identity=28.2131661442006, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24650754, Length=319, Percent_Identity=28.2131661442006, Blast_Score=124, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24650752, Length=319, Percent_Identity=28.2131661442006, Blast_Score=123, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002123
- InterPro:   IPR022284 [H]

Pfam domain/function: PF01553 Acyltransferase [H]

EC number: =2.3.1.15 [H]

Molecular weight: Translated: 90826; Mature: 90826

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSMSSGHLLSRSLLKLPMSVLVKGTAIPSNPIQDLDIDTHKPVIYALPFRSNVDLLTLQ
CCCCCCCHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEE
THAKEAGLPDPLEPLMLNGKAFQRYVFIASRPTLLSSDQHVPSDSIALFSELLTEHKLDS
HHHHHCCCCCCCHHHHCCCHHHEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
ELDVQVIPATVLWGRKPGKEGQERPYLQALNGPEKALAVLASGRDCLVRFSPVVSMRYMA
CCCEEEECHHEEECCCCCCCCCCCCHHHHCCCHHHHHHHHHCCCHHEEEECHHHHHHHHH
DTHGTDASIAHKLARVARIHFSRQKLAASGPNLPQRAQLFARLMNSPAIEKAIADEAKSK
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCC
QIPLEKARKEAHDILDEIAADFSYSLVKKGDRILGWLWNRIYQGLNINNAATVRRLAQDG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCC
HEIVYVPCHRSHMDYLLLSYVLYHEGMVPPHIAAGINLNFFPAGPIFRRGGAFFIRRSFK
CEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCEEEECCCCHHHCCCCEEEEECCC
GAPLYSTIFREYLAELFAKGYSVEYFSEGGRSRTGRLLPAKTGMLAMTIQAMLRGLNRPV
CCCHHHHHHHHHHHHHHHCCCCEEHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCE
TLVPVYIGYEHVMEVGTYAKELRGKRKEKENAGLVLRTLRKLRNFGQGYVNFGEPIPLNQ
EEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCHHH
FLNETVPQWTQDIDPMGESKPQWMTPTVNKLANRMMTHINDAAAVNAMTLCATALLASRQ
HHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
RALARDNLIKQVDCYLSLLRNVPYSATSTLPSESAEKLVEHAESLDKFVVETDTMGDIIS
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHC
LDRNQSILMTYYRNNIIHLLALPSLIAQLLIRQQSVSLEKVQATVAQIYPFLKQELFLRF
CCCCCCEEEEEHHCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHC
EAEELNDLVLRYVAELARQGLVTVEGKTVTLNQAQTQVLMLLGRIISETLQRYAIALNLL
CHHHHHHHHHHHHHHHHHCCCEEECCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSCPHLGKAELEEKSQEVAQRLGRLHGINAPEFFDKGVFASLFVTLQEQGYLDDQGRCVL
HCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHCHHHHHHHHHHHHCCCCCCCCCEEE
ETAKPLSRQLYALIYPEVRMTIQESLCQVDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNSMSSGHLLSRSLLKLPMSVLVKGTAIPSNPIQDLDIDTHKPVIYALPFRSNVDLLTLQ
CCCCCCCHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEE
THAKEAGLPDPLEPLMLNGKAFQRYVFIASRPTLLSSDQHVPSDSIALFSELLTEHKLDS
HHHHHCCCCCCCHHHHCCCHHHEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
ELDVQVIPATVLWGRKPGKEGQERPYLQALNGPEKALAVLASGRDCLVRFSPVVSMRYMA
CCCEEEECHHEEECCCCCCCCCCCCHHHHCCCHHHHHHHHHCCCHHEEEECHHHHHHHHH
DTHGTDASIAHKLARVARIHFSRQKLAASGPNLPQRAQLFARLMNSPAIEKAIADEAKSK
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCC
QIPLEKARKEAHDILDEIAADFSYSLVKKGDRILGWLWNRIYQGLNINNAATVRRLAQDG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCC
HEIVYVPCHRSHMDYLLLSYVLYHEGMVPPHIAAGINLNFFPAGPIFRRGGAFFIRRSFK
CEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCEEEECCCCHHHCCCCEEEEECCC
GAPLYSTIFREYLAELFAKGYSVEYFSEGGRSRTGRLLPAKTGMLAMTIQAMLRGLNRPV
CCCHHHHHHHHHHHHHHHCCCCEEHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCE
TLVPVYIGYEHVMEVGTYAKELRGKRKEKENAGLVLRTLRKLRNFGQGYVNFGEPIPLNQ
EEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCHHH
FLNETVPQWTQDIDPMGESKPQWMTPTVNKLANRMMTHINDAAAVNAMTLCATALLASRQ
HHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
RALARDNLIKQVDCYLSLLRNVPYSATSTLPSESAEKLVEHAESLDKFVVETDTMGDIIS
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHC
LDRNQSILMTYYRNNIIHLLALPSLIAQLLIRQQSVSLEKVQATVAQIYPFLKQELFLRF
CCCCCCEEEEEHHCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHC
EAEELNDLVLRYVAELARQGLVTVEGKTVTLNQAQTQVLMLLGRIISETLQRYAIALNLL
CHHHHHHHHHHHHHHHHHCCCEEECCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSCPHLGKAELEEKSQEVAQRLGRLHGINAPEFFDKGVFASLFVTLQEQGYLDDQGRCVL
HCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHCHHHHHHHHHHHHCCCCCCCCCEEE
ETAKPLSRQLYALIYPEVRMTIQESLCQVDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA