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The map label for this gene is pheS

Identifier: 226950561

GI number: 226950561

Start: 3574320

End: 3575339

Strand: Reverse

Name: pheS

Synonym: CLM_3538

Alternate gene names: 226950561

Gene position: 3575339-3574320 (Counterclockwise)

Preceding gene: 226950562

Following gene: 226950560

Centisome position: 86.04

GC content: 30.69

Gene sequence:

>1020_bases
ATGCGACAAAAATTAGAAGAGATTAAAAATAGTGCAATAAATGAATTAAAAACAACTTTAAGTAAAGATCAATTAGAAGC
TATAAGAGTAAAATATTTAGGAAAAAAGGGTGAACTCACACAAATATTAAGAGGTATGGGAGCTTTATCTCAAGAGGAAA
GACCTATTGTAGGTAAAGTTGCTAATGAAGTAAGATCATATATAGAAGAAACTATAAAAGAAGCTTTCTCTGATATAAAA
AATAAAGAAAAATCTATAAGATTAGAAAATGAAACTATAGACATAACTATGCCAGGAAAAAAACAAGCAGTAGGAAAAAG
ACATCCTCTAGATTTAACATTAGAAAGTATGAAAGATATATTTATATCCATGGGATTTACAATTGAGGAAGGTCCAGAAG
TTGAACTTGATAAGTATAATTTTGAAGCATTGAATATACCAAAGAATCATCCAGCAAGAGGAGAACAAGATACTTTTTAT
ATAAATGATAATTTGGTCTTGAGAACTCAAACATCTCCAATACAAATAAGAACTATGGAAAATCAAAAACCTCCTATTAA
GATGATAGCTCCGGGAAAAGTTTATCGTTCTGATTCAGTGGATGCTACTCATTCACCTATATTTTATCAAATGGAAGGGT
TAGTAGTAGATAAGGGAATAACTTTTTCAGATTTAAAAGGTACATTAGAGTTATTTGCAAAAAGAATGTTCGGGGATAAG
GTTAAAACTAAGTTTAGACCACATCATTTCCCATTTACAGAACCTTCAGCAGAAATGGATGCTACATGCTTTGTATGCAA
TGGAGAGGGCTGTAAAGTGTGTAAAGGTAGTGGTTGGATTGAACTTTTAGGTTGTGGAATGGTTCATCCACAAGTTCTTA
GAAACTGTAATATAGATCCAGAAGTTTATAGTGGATTTGCATTTGGATTCGGAGTAGATAGAATGGTTATGATGAAATAT
GGCATAGATGATATAAGGCTTTTATATGAAAGTGATATGAGATTTTTAAATCAATTTTAA

Upstream 100 bases:

>100_bases
TAGGGTGGTACCGCGGATTTAGTTCGTCCCTTTATGGGATGGACTTTTTTATTATAAAAATTTTTACAGCTAATATAAAA
ATGGAAAGGAGTATTGTGTT

Downstream 100 bases:

>100_bases
ATCTATACTTTAATTTTAAAAATTAAATAATTACAACAGGTTACAGGTATAAAGGCGTTATAGACATACACTTCTAGTTT
AGTTGTATAAAATAGAAGTT

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MRQKLEEIKNSAINELKTTLSKDQLEAIRVKYLGKKGELTQILRGMGALSQEERPIVGKVANEVRSYIEETIKEAFSDIK
NKEKSIRLENETIDITMPGKKQAVGKRHPLDLTLESMKDIFISMGFTIEEGPEVELDKYNFEALNIPKNHPARGEQDTFY
INDNLVLRTQTSPIQIRTMENQKPPIKMIAPGKVYRSDSVDATHSPIFYQMEGLVVDKGITFSDLKGTLELFAKRMFGDK
VKTKFRPHHFPFTEPSAEMDATCFVCNGEGCKVCKGSGWIELLGCGMVHPQVLRNCNIDPEVYSGFAFGFGVDRMVMMKY
GIDDIRLLYESDMRFLNQF

Sequences:

>Translated_339_residues
MRQKLEEIKNSAINELKTTLSKDQLEAIRVKYLGKKGELTQILRGMGALSQEERPIVGKVANEVRSYIEETIKEAFSDIK
NKEKSIRLENETIDITMPGKKQAVGKRHPLDLTLESMKDIFISMGFTIEEGPEVELDKYNFEALNIPKNHPARGEQDTFY
INDNLVLRTQTSPIQIRTMENQKPPIKMIAPGKVYRSDSVDATHSPIFYQMEGLVVDKGITFSDLKGTLELFAKRMFGDK
VKTKFRPHHFPFTEPSAEMDATCFVCNGEGCKVCKGSGWIELLGCGMVHPQVLRNCNIDPEVYSGFAFGFGVDRMVMMKY
GIDDIRLLYESDMRFLNQF
>Mature_339_residues
MRQKLEEIKNSAINELKTTLSKDQLEAIRVKYLGKKGELTQILRGMGALSQEERPIVGKVANEVRSYIEETIKEAFSDIK
NKEKSIRLENETIDITMPGKKQAVGKRHPLDLTLESMKDIFISMGFTIEEGPEVELDKYNFEALNIPKNHPARGEQDTFY
INDNLVLRTQTSPIQIRTMENQKPPIKMIAPGKVYRSDSVDATHSPIFYQMEGLVVDKGITFSDLKGTLELFAKRMFGDK
VKTKFRPHHFPFTEPSAEMDATCFVCNGEGCKVCKGSGWIELLGCGMVHPQVLRNCNIDPEVYSGFAFGFGVDRMVMMKY
GIDDIRLLYESDMRFLNQF

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5729820, Length=289, Percent_Identity=29.0657439446367, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI4758340, Length=271, Percent_Identity=30.2583025830258, Blast_Score=117, Evalue=1e-26,
Organism=Escherichia coli, GI1788007, Length=335, Percent_Identity=46.865671641791, Blast_Score=340, Evalue=1e-94,
Organism=Caenorhabditis elegans, GI32566635, Length=256, Percent_Identity=31.640625, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI32563657, Length=273, Percent_Identity=27.8388278388278, Blast_Score=111, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17508957, Length=273, Percent_Identity=27.8388278388278, Blast_Score=111, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6321087, Length=274, Percent_Identity=31.021897810219, Blast_Score=126, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6325304, Length=144, Percent_Identity=32.6388888888889, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI18858079, Length=270, Percent_Identity=30.7407407407407, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI17137424, Length=249, Percent_Identity=27.710843373494, Blast_Score=108, Evalue=7e-24,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_CLOB1 (A7FY80)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001385453.1
- ProteinModelPortal:   A7FY80
- SMR:   A7FY80
- STRING:   A7FY80
- GeneID:   5397608
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_3161
- eggNOG:   COG0016
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- ProtClustDB:   PRK00488
- BioCyc:   CBOT441770:CLB_3161-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 38585; Mature: 38585

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQKLEEIKNSAINELKTTLSKDQLEAIRVKYLGKKGELTQILRGMGALSQEERPIVGKV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCHHHHH
ANEVRSYIEETIKEAFSDIKNKEKSIRLENETIDITMPGKKQAVGKRHPLDLTLESMKDI
HHHHHHHHHHHHHHHHHHHHCCCCCEEEECCEEEEEECCCHHHCCCCCCCCEEHHHHHHH
FISMGFTIEEGPEVELDKYNFEALNIPKNHPARGEQDTFYINDNLVLRTQTSPIQIRTME
HHHCCCEECCCCCEEEECCCCEEECCCCCCCCCCCCCEEEECCCEEEEECCCCEEEEECC
NQKPPIKMIAPGKVYRSDSVDATHSPIFYQMEGLVVDKGITFSDLKGTLELFAKRMFGDK
CCCCCEEEECCCCEECCCCCCCCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHCHH
VKTKFRPHHFPFTEPSAEMDATCFVCNGEGCKVCKGSGWIELLGCGMVHPQVLRNCNIDP
HHHCCCCCCCCCCCCCCCCCCEEEEECCCCCEEECCCCEEEEEECCCCCHHHHHCCCCCH
EVYSGFAFGFGVDRMVMMKYGIDDIRLLYESDMRFLNQF
HHHCCCHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRQKLEEIKNSAINELKTTLSKDQLEAIRVKYLGKKGELTQILRGMGALSQEERPIVGKV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCHHHHH
ANEVRSYIEETIKEAFSDIKNKEKSIRLENETIDITMPGKKQAVGKRHPLDLTLESMKDI
HHHHHHHHHHHHHHHHHHHHCCCCCEEEECCEEEEEECCCHHHCCCCCCCCEEHHHHHHH
FISMGFTIEEGPEVELDKYNFEALNIPKNHPARGEQDTFYINDNLVLRTQTSPIQIRTME
HHHCCCEECCCCCEEEECCCCEEECCCCCCCCCCCCCEEEECCCEEEEECCCCEEEEECC
NQKPPIKMIAPGKVYRSDSVDATHSPIFYQMEGLVVDKGITFSDLKGTLELFAKRMFGDK
CCCCCEEEECCCCEECCCCCCCCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHCHH
VKTKFRPHHFPFTEPSAEMDATCFVCNGEGCKVCKGSGWIELLGCGMVHPQVLRNCNIDP
HHHCCCCCCCCCCCCCCCCCCEEEEECCCCCEEECCCCEEEEEECCCCCHHHHHCCCCCH
EVYSGFAFGFGVDRMVMMKYGIDDIRLLYESDMRFLNQF
HHHCCCHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA