| Definition | Clostridium botulinum A2 str. Kyoto chromosome, complete genome. |
|---|---|
| Accession | NC_012563 |
| Length | 4,155,278 |
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The map label for this gene is amiA [C]
Identifier: 226950541
GI number: 226950541
Start: 3543504
End: 3546098
Strand: Reverse
Name: amiA [C]
Synonym: CLM_3517
Alternate gene names: 226950541
Gene position: 3546098-3543504 (Counterclockwise)
Preceding gene: 226950542
Following gene: 226950540
Centisome position: 85.34
GC content: 27.55
Gene sequence:
>2595_bases ATGAAGAATAAAAAATTCTATTTAGTACCTAAGATTATTTCAATATTTTTTATGTTATTTTTATTTTCTATACAAGCACA AGCTGTATCGAATATAAGTATTAATTTAAAAACACCAACAAATCAAAGTATAATAAATAGTAGTGAGTTAAATATTGATG GAGAAATAAAATCTATTTTAAAGTTAAAATCAGTATATTTATATATAGATAATGAAAAAATAGGACAAGCAAATTTAGGT GAATTAGAATTTAAAGAAAATACATATAAAACTAGATTAAACTATACAAAAAATATTTCTTCTTTGAAAAATGGAATACA TAATTTAAGAATTTTAGCTATAGATGAACAGAATAACAAAGAAGAAAAAGAAATTAGTATAAATATACAAGAAAATCAAG AAAAATCAGAAACTGATAAAAATATTATACAGAATAATTTAGTAGATACTAATGCAAAGATGGGTAGTATAGAAAAAGTA TTAACTACTGTATCGAATGAAGCTAAACTAGAAAAAGTAGAAGTTTCATACAATGGTAATGTAATAACTAATGGAGAAAT AGGAGTTGGAAAAAGCTACGTTATAAAGGGATATGGAAATTCTGAAAATGGAGTACTGTATCAATTTTGGGTAAAGGATC TATCTACAAACAGTTGGACAATGATAAGAGATTATGGAGAAACAAATAGTTTTAATTACACACCAACAAAGGCTGGAAAG TATCTGATAGGAATACATGTAAAAGATAAGTATAGTAAAGAAAATTTAGATGATTTTATATATGAGAATTATACAGTAAC TGTAAGTAAAGCTAAATTAGAAAAAGTGGAAGTTTCATACAATGGTAGCGTGATAACTGATGGAGAAATAGGAGTTGGAA AAAGCTACGTTATAAAAGGATATGGAAATTCTGAAAATGGAGTACTGTATCAATTTTGGGTAAAGGATCTATCCACAAAT AGTTGGACAATGATAAGAGATTATGGAGAAACAAATAGTTTTAATTATACACCAGCAAAGGCTGGAAAGTATCTGATAGG AATACATGTAAAGGATAAGTATAGTAAAGAGAATTTAGATGATTTTATATATGAAAATTATACAGTAACTATAAGTAAAG CTAAATTAGAAAAAGTGGAAGTTTCATACGATGGTAATGTAATAACTAATGGAGAAATAGGAGTTGGAAAAAGCTACGTT ATAAAAGGATATGGAAATTCTGAAAATGGAGTATTGTATCAATTTTGGGTAAAGGATTTATCTACAAACAGTTGGACAAT GATAAGAGATTATGGAGAAACAAATAGTTTTAATTATACACCAGCAAAGGATGGAAAGTATTTGATAGGAATACATGTAA AAGATAAGTATAGTAAAGAGAATTTAGATGATTTTATATATGAAAATTATACAGTAACTATAAGTAAAGCTAAATTAGAA AAAGTAGAGATTTCATATAATGGTAATGTGATAACCAATGGAGAAATAGGAGTTGGAAAAAGCTACGTTATAAAAGGATA TGGAAATTCTGCAAATGGAGTATTGTATCAATTTTGGGTAAAAGATCTATCTACAAACAGTTGGACAATGATAAAAGATT ATGGAGAAGAGAATAGTATAAATTGGAAACCTACTGTTGGTGGAAAATATTTAGTAGGGATACATGTAAAGGATAAATAT AGTATCGATACATTAGATGATCACATATATGAGAATTATACAATATTGAGTGATAATGCAAGGCTAGAAAAGGTAGAAGT TAAATTAGATGGAAACTTAATAAGTAATAATTTAAATGTAGGAAAGACATATACAATAGAAGGAAGTGCTACATCTAAAA ATGGAGTGCTATATCAATTTTGGGTAAAAGACTTGTCTATAAATAGCTGGGCAATGTTAAGGGATTATGGAGAATCAAAT AATATAACATATACACCAACAAAGAATGGACAATATTTAATAGGGGTACATGTAAAAGATAAGAATAGCAAGGAAAGCTT AGATGATTTTAAATACAATCAATATAGCATTATAGGTTATAATAAAACAGTAATTGTTGATGCAGGACATGGAGGATATG ATAGTGGCGCTGTAGGACCAACCGGTATTAAAGAAAAGGATGTCACTTTAAAAGTAGCTTTAAAGTTAGGAAAAATTTTA GAAAATAGTGGGGTAAATGTTATTTATACTAGAACTAGTGATAATGTATCTTGGCCTTCAAATGAATCACAAGATTTAGC AGCTAGAGTGGCCATTGCAAATAACAATAATACAGATTTATATGTAAGTATACATGCTAATAGTTTTAATGGATCAGCGA ATGGAACAGAAACATATTACTATGATGGGAGTGCAAAAGGAAAAGAAACAGCAGAAAAAGTTCAAAAGGAACTTGTAAAT TCTATTGGCTTATATGATAGAGGCGTTAAAACAGCAGGATATTATGTTTTAAAAAATACTATAAGCCCATCTATATTGGT TGAATTAGGATTCATAGATAATAGAAATGAAGAAATTCTTTTAAATTCTGATTGGTTTCAACAAAAGTGTGCCGAAGCTA TTGCTAAAGGAATATTATCAACTAGCTTTATGTAA
Upstream 100 bases:
>100_bases AAAGTTCAATAAAAATAATAAATATTATTATAATTTTATTTTAAGTATAGAGTTCACGCTAAGTAAATTTATAAATATAA TTATTGAAAGGATTGAAGAG
Downstream 100 bases:
>100_bases CCTAATGAATATAAAAAGTAATAAATCAATAAAATAATTATAAAATTGCATATTAAGCTCTAATTATGCTAGTCTTAAAA TAAATAAAAAACCATTAATT
Product: N-acetylmuramoyl-L-alanine amidase
Products: Hydrolyzed cell wall glycopeptides; N-acetylmuramoyl residues; L-amino acid residues [C]
Alternate protein names: NA
Number of amino acids: Translated: 864; Mature: 864
Protein sequence:
>864_residues MKNKKFYLVPKIISIFFMLFLFSIQAQAVSNISINLKTPTNQSIINSSELNIDGEIKSILKLKSVYLYIDNEKIGQANLG ELEFKENTYKTRLNYTKNISSLKNGIHNLRILAIDEQNNKEEKEISINIQENQEKSETDKNIIQNNLVDTNAKMGSIEKV LTTVSNEAKLEKVEVSYNGNVITNGEIGVGKSYVIKGYGNSENGVLYQFWVKDLSTNSWTMIRDYGETNSFNYTPTKAGK YLIGIHVKDKYSKENLDDFIYENYTVTVSKAKLEKVEVSYNGSVITDGEIGVGKSYVIKGYGNSENGVLYQFWVKDLSTN SWTMIRDYGETNSFNYTPAKAGKYLIGIHVKDKYSKENLDDFIYENYTVTISKAKLEKVEVSYDGNVITNGEIGVGKSYV IKGYGNSENGVLYQFWVKDLSTNSWTMIRDYGETNSFNYTPAKDGKYLIGIHVKDKYSKENLDDFIYENYTVTISKAKLE KVEISYNGNVITNGEIGVGKSYVIKGYGNSANGVLYQFWVKDLSTNSWTMIKDYGEENSINWKPTVGGKYLVGIHVKDKY SIDTLDDHIYENYTILSDNARLEKVEVKLDGNLISNNLNVGKTYTIEGSATSKNGVLYQFWVKDLSINSWAMLRDYGESN NITYTPTKNGQYLIGVHVKDKNSKESLDDFKYNQYSIIGYNKTVIVDAGHGGYDSGAVGPTGIKEKDVTLKVALKLGKIL ENSGVNVIYTRTSDNVSWPSNESQDLAARVAIANNNNTDLYVSIHANSFNGSANGTETYYYDGSAKGKETAEKVQKELVN SIGLYDRGVKTAGYYVLKNTISPSILVELGFIDNRNEEILLNSDWFQQKCAEAIAKGILSTSFM
Sequences:
>Translated_864_residues MKNKKFYLVPKIISIFFMLFLFSIQAQAVSNISINLKTPTNQSIINSSELNIDGEIKSILKLKSVYLYIDNEKIGQANLG ELEFKENTYKTRLNYTKNISSLKNGIHNLRILAIDEQNNKEEKEISINIQENQEKSETDKNIIQNNLVDTNAKMGSIEKV LTTVSNEAKLEKVEVSYNGNVITNGEIGVGKSYVIKGYGNSENGVLYQFWVKDLSTNSWTMIRDYGETNSFNYTPTKAGK YLIGIHVKDKYSKENLDDFIYENYTVTVSKAKLEKVEVSYNGSVITDGEIGVGKSYVIKGYGNSENGVLYQFWVKDLSTN SWTMIRDYGETNSFNYTPAKAGKYLIGIHVKDKYSKENLDDFIYENYTVTISKAKLEKVEVSYDGNVITNGEIGVGKSYV IKGYGNSENGVLYQFWVKDLSTNSWTMIRDYGETNSFNYTPAKDGKYLIGIHVKDKYSKENLDDFIYENYTVTISKAKLE KVEISYNGNVITNGEIGVGKSYVIKGYGNSANGVLYQFWVKDLSTNSWTMIKDYGEENSINWKPTVGGKYLVGIHVKDKY SIDTLDDHIYENYTILSDNARLEKVEVKLDGNLISNNLNVGKTYTIEGSATSKNGVLYQFWVKDLSINSWAMLRDYGESN NITYTPTKNGQYLIGVHVKDKNSKESLDDFKYNQYSIIGYNKTVIVDAGHGGYDSGAVGPTGIKEKDVTLKVALKLGKIL ENSGVNVIYTRTSDNVSWPSNESQDLAARVAIANNNNTDLYVSIHANSFNGSANGTETYYYDGSAKGKETAEKVQKELVN SIGLYDRGVKTAGYYVLKNTISPSILVELGFIDNRNEEILLNSDWFQQKCAEAIAKGILSTSFM >Mature_864_residues MKNKKFYLVPKIISIFFMLFLFSIQAQAVSNISINLKTPTNQSIINSSELNIDGEIKSILKLKSVYLYIDNEKIGQANLG ELEFKENTYKTRLNYTKNISSLKNGIHNLRILAIDEQNNKEEKEISINIQENQEKSETDKNIIQNNLVDTNAKMGSIEKV LTTVSNEAKLEKVEVSYNGNVITNGEIGVGKSYVIKGYGNSENGVLYQFWVKDLSTNSWTMIRDYGETNSFNYTPTKAGK YLIGIHVKDKYSKENLDDFIYENYTVTVSKAKLEKVEVSYNGSVITDGEIGVGKSYVIKGYGNSENGVLYQFWVKDLSTN SWTMIRDYGETNSFNYTPAKAGKYLIGIHVKDKYSKENLDDFIYENYTVTISKAKLEKVEVSYDGNVITNGEIGVGKSYV IKGYGNSENGVLYQFWVKDLSTNSWTMIRDYGETNSFNYTPAKDGKYLIGIHVKDKYSKENLDDFIYENYTVTISKAKLE KVEISYNGNVITNGEIGVGKSYVIKGYGNSANGVLYQFWVKDLSTNSWTMIKDYGEENSINWKPTVGGKYLVGIHVKDKY SIDTLDDHIYENYTILSDNARLEKVEVKLDGNLISNNLNVGKTYTIEGSATSKNGVLYQFWVKDLSINSWAMLRDYGESN NITYTPTKNGQYLIGVHVKDKNSKESLDDFKYNQYSIIGYNKTVIVDAGHGGYDSGAVGPTGIKEKDVTLKVALKLGKIL ENSGVNVIYTRTSDNVSWPSNESQDLAARVAIANNNNTDLYVSIHANSFNGSANGTETYYYDGSAKGKETAEKVQKELVN SIGLYDRGVKTAGYYVLKNTISPSILVELGFIDNRNEEILLNSDWFQQKCAEAIAKGILSTSFM
Specific function: Unknown
COG id: COG0860
COG function: function code M; N-acetylmuramoyl-L-alanine amidase
Gene ontology:
Cell location: Secreted, cell wall, S-layer (Potential) [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 3 SLH (S-layer homology) domains [H]
Homologues:
Organism=Escherichia coli, GI1788776, Length=228, Percent_Identity=32.4561403508772, Blast_Score=104, Evalue=3e-23, Organism=Escherichia coli, GI87082163, Length=220, Percent_Identity=31.8181818181818, Blast_Score=98, Evalue=2e-21,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002508 - InterPro: IPR001119 [H]
Pfam domain/function: PF01520 Amidase_3; PF00395 SLH [H]
EC number: 3.5.1.28 [C]
Molecular weight: Translated: 97082; Mature: 97082
Theoretical pI: Translated: 6.15; Mature: 6.15
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.1 %Cys (Translated Protein) 1.0 %Met (Translated Protein) 1.2 %Cys+Met (Translated Protein) 0.1 %Cys (Mature Protein) 1.0 %Met (Mature Protein) 1.2 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKNKKFYLVPKIISIFFMLFLFSIQAQAVSNISINLKTPTNQSIINSSELNIDGEIKSIL CCCCEEEEHHHHHHHHHHHHHHHHHHHEECEEEEEEECCCCCCEECCCCCCCCHHHHHHH KLKSVYLYIDNEKIGQANLGELEFKENTYKTRLNYTKNISSLKNGIHNLRILAIDEQNNK EEEEEEEEECCCCCCCCCCCCEEECCCCEEEEEEECCCHHHHHCCCCEEEEEEEECCCCC EEKEISINIQENQEKSETDKNIIQNNLVDTNAKMGSIEKVLTTVSNEAKLEKVEVSYNGN CCEEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEEEECCC VITNGEIGVGKSYVIKGYGNSENGVLYQFWVKDLSTNSWTMIRDYGETNSFNYTPTKAGK EEECCCCCCCCEEEEEECCCCCCCEEEEEEEECCCCCCEEEEEECCCCCCCCCCCCCCCC YLIGIHVKDKYSKENLDDFIYENYTVTVSKAKLEKVEVSYNGSVITDGEIGVGKSYVIKG EEEEEEEECCCCCCCCHHHHHCCEEEEEEECEEEEEEEEECCCEEECCCCCCCCEEEEEE YGNSENGVLYQFWVKDLSTNSWTMIRDYGETNSFNYTPAKAGKYLIGIHVKDKYSKENLD CCCCCCCEEEEEEEECCCCCCEEEEEECCCCCCCCCCHHCCCCEEEEEEEECCCCCCCCH DFIYENYTVTISKAKLEKVEVSYDGNVITNGEIGVGKSYVIKGYGNSENGVLYQFWVKDL HHHHCCEEEEEEECEEEEEEEEECCCEEECCCCCCCCEEEEEECCCCCCCEEEEEEEECC STNSWTMIRDYGETNSFNYTPAKDGKYLIGIHVKDKYSKENLDDFIYENYTVTISKAKLE CCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHCCEEEEEEEEEEE KVEISYNGNVITNGEIGVGKSYVIKGYGNSANGVLYQFWVKDLSTNSWTMIKDYGEENSI EEEEEECCCEEECCCCCCCCEEEEEECCCCCCCEEEEEEEHHCCCCCEEEEEECCCCCCC NWKPTVGGKYLVGIHVKDKYSIDTLDDHIYENYTILSDNARLEKVEVKLDGNLISNNLNV EEEECCCCEEEEEEEEECCCCCCCHHHHHHCCEEEEECCCEEEEEEEEECCEEEECCCCC GKTYTIEGSATSKNGVLYQFWVKDLSINSWAMLRDYGESNNITYTPTKNGQYLIGVHVKD CEEEEEECCCCCCCCEEEEEEEECCCCCCEEEEEECCCCCCEEEEECCCCCEEEEEEEEC KNSKESLDDFKYNQYSIIGYNKTVIVDAGHGGYDSGAVGPTGIKEKDVTLKVALKLGKIL CCCCCCHHHCCCCCEEEEECCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHHH ENSGVNVIYTRTSDNVSWPSNESQDLAARVAIANNNNTDLYVSIHANSFNGSANGTETYY HCCCCEEEEEECCCCCCCCCCCCCCEEEEEEEECCCCCEEEEEEEECCCCCCCCCCEEEE YDGSAKGKETAEKVQKELVNSIGLYDRGVKTAGYYVLKNTISPSILVELGFIDNRNEEIL ECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCEEEEEEEEECCCCCEEE LNSDWFQQKCAEAIAKGILSTSFM ECCHHHHHHHHHHHHHHHHHHCCC >Mature Secondary Structure MKNKKFYLVPKIISIFFMLFLFSIQAQAVSNISINLKTPTNQSIINSSELNIDGEIKSIL CCCCEEEEHHHHHHHHHHHHHHHHHHHEECEEEEEEECCCCCCEECCCCCCCCHHHHHHH KLKSVYLYIDNEKIGQANLGELEFKENTYKTRLNYTKNISSLKNGIHNLRILAIDEQNNK EEEEEEEEECCCCCCCCCCCCEEECCCCEEEEEEECCCHHHHHCCCCEEEEEEEECCCCC EEKEISINIQENQEKSETDKNIIQNNLVDTNAKMGSIEKVLTTVSNEAKLEKVEVSYNGN CCEEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEEEECCC VITNGEIGVGKSYVIKGYGNSENGVLYQFWVKDLSTNSWTMIRDYGETNSFNYTPTKAGK EEECCCCCCCCEEEEEECCCCCCCEEEEEEEECCCCCCEEEEEECCCCCCCCCCCCCCCC YLIGIHVKDKYSKENLDDFIYENYTVTVSKAKLEKVEVSYNGSVITDGEIGVGKSYVIKG EEEEEEEECCCCCCCCHHHHHCCEEEEEEECEEEEEEEEECCCEEECCCCCCCCEEEEEE YGNSENGVLYQFWVKDLSTNSWTMIRDYGETNSFNYTPAKAGKYLIGIHVKDKYSKENLD CCCCCCCEEEEEEEECCCCCCEEEEEECCCCCCCCCCHHCCCCEEEEEEEECCCCCCCCH DFIYENYTVTISKAKLEKVEVSYDGNVITNGEIGVGKSYVIKGYGNSENGVLYQFWVKDL HHHHCCEEEEEEECEEEEEEEEECCCEEECCCCCCCCEEEEEECCCCCCCEEEEEEEECC STNSWTMIRDYGETNSFNYTPAKDGKYLIGIHVKDKYSKENLDDFIYENYTVTISKAKLE CCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHCCEEEEEEEEEEE KVEISYNGNVITNGEIGVGKSYVIKGYGNSANGVLYQFWVKDLSTNSWTMIKDYGEENSI EEEEEECCCEEECCCCCCCCEEEEEECCCCCCCEEEEEEEHHCCCCCEEEEEECCCCCCC NWKPTVGGKYLVGIHVKDKYSIDTLDDHIYENYTILSDNARLEKVEVKLDGNLISNNLNV EEEECCCCEEEEEEEEECCCCCCCHHHHHHCCEEEEECCCEEEEEEEEECCEEEECCCCC GKTYTIEGSATSKNGVLYQFWVKDLSINSWAMLRDYGESNNITYTPTKNGQYLIGVHVKD CEEEEEECCCCCCCCEEEEEEEECCCCCCEEEEEECCCCCCEEEEECCCCCEEEEEEEEC KNSKESLDDFKYNQYSIIGYNKTVIVDAGHGGYDSGAVGPTGIKEKDVTLKVALKLGKIL CCCCCCHHHCCCCCEEEEECCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHHH ENSGVNVIYTRTSDNVSWPSNESQDLAARVAIANNNNTDLYVSIHANSFNGSANGTETYY HCCCCEEEEEECCCCCCCCCCCCCCEEEEEEEECCCCCEEEEEEEECCCCCCCCCCEEEE YDGSAKGKETAEKVQKELVNSIGLYDRGVKTAGYYVLKNTISPSILVELGFIDNRNEEIL ECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCEEEEEEEEECCCCCEEE LNSDWFQQKCAEAIAKGILSTSFM ECCHHHHHHHHHHHHHHHHHHCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: cell wall glycopeptides; D-lactyl-L-Ala [C]
Specific reaction: Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues [C]
General reaction: Carboxylic acid amide hydrolysis [C]
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 10475962; 12004073 [H]